• Korean Journal of Plant Resources
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• v.21 no.5
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• pp.388-394
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• 2008

This study was carried out to develop new cultivars using the $T_5$ generation of transformed rice by PCR analysis with DNA marker in each generation $(from\;T_3\;to\;T_5)$. In the previous study, we successfully developed the transgenic rice plants over-expressing the Arabidopsis $H^+/Ca^{2+}$ antiporter CAX 1 (accession no. U57411) gene. The calcium concentration in brown rice of transgenic plants was higher than that of donor plants, Iipum, and was selected 3 lines out of 25 lines at cultured GMO field. The major agronomic traits such as culm length, panicle length and panicle number of 3 lines at transgenic plants $(T_5)$ were similar to wild type. Also these lines appeared to have disease resistance to rice blast, cold resistance as compared with donor types. The grain shape was similar to donor plant, however, the 1000 grain weight of brown rice was different from transgenic plants. These finding would be used for basic data of new variety registration.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.4
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• pp.465-474
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• 1996

The study was carried out to determine the proper concentration of plant growth regulator, kinetin on alleviating copper toxicity for two rice cultivars of seed germination. The results were as followings : Soaking treatment of kinetin 10$^{-3}$ M increased the germination rate of both cultivars, Ilpumbyeo and Hyangmibyeo 1 by 92% and 88% as compared with copper treatment (60ppm). But the soaking treatment effect of plant growth regulator, kinetin was not recognized at the kinetin 10$^{-4}$ M and 10$^{-5}$ M. Chlorophyll content of both rices was higher than that of Hyangmibyeo 1. Copper content of Ilpumbyeo was higher in leaf than in seed part. At the 3 days after treatment of copper 60ppm, both cultivars of treatment of kinetin 1O-3M showed the somewhat thin bands at the 35 and 40kDa compared with others. A new protein band pattern was only appeared to kinetin 1O-3M at approximately 54.4kDa(M. W) at the 7 days after treatment of copper 60ppm in llpumbyeo cultivar, SOD activity of copper 60ppm treatment increased in 3DAT, but there were no significant differences in 5 and 7DAT of two cultivars. Free proline contents of copper 60ppm treatment in llpumbyeo were remarkably increased about 4.996$\mu$M. In particular, free proline content of kinetin l0$^{-3}$ M in Ilpumbyeo was 5.008$\mu$M in 3DAT. In case of Hwangmibyeo 1, free proline content of copper 60ppm was 5.825$\mu$M compared with an untreated control showing 2.34l$\mu$M. The effects of kinetin treatment were recognized to promote the root growth and germination rate under copper toxicity(60ppm) condition in both cultivars.

• Food Science and Preservation
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• v.22 no.1
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• pp.63-69
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• 2015

The highly producible rice cultivars, hanareum and dasan 2, were prepared and investigated for their physicochemical properties, and the quality characteristics of frozen cookie dough with rice flour according to the thawing time were measured. The water, ash, crude protein, amylose, and damaged starch contents of rice flour, as well as its water holding capacity, particle size, and Hunter color value, were measured. The water, ash, and crude protein contents of the hanareum and dasan 2 rice flours were shown to range from 7.28 to 13.14%, from 0.35 to 0.39, and from 6.05 and 8.68%, respectively. The protein content of the control group was higher than that of hanareum and dasan 2 rice flours. The amylose contents of the hanareum and dasan 2 rice flour were 19.05 and 23.04%, respectively. The damaged starch content and water holding capacity of the control group were lower than those of the hanareum and dasan 2 rice flours. The particle sizes of the samples were $48.54{\sim}50.05{\mu}m$. The lightness values of the hanareum, and dasan 2 rice flour, and of the control were 93.72, 93.51 and 92.63, respectively. The quality characteristics of the gluten-free frozen cookie dough were investigated. The lightness of the cookie made with frozen cookie dough decreased according to the by thawing time, but the diameter of the cookie did not differ significantly. The hardness of the cookie made with rice frozen dasan 2 rice dough was lower than that of the cookie made with frozen hanareum rice dough.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.279-279
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• 2017

In Korea, the single span unheated plastic house cultivated crops from autumn to spring of the following year, removed the plastic film and frame, cultivated rice, set up a plastic house again and cultivated crops. The crops in the greenhouse are utilized mainly for the production of leaf vegetables such as lettuce, leek, and fruit vegetables such as strawberry, watermelon, oriental melon, etc. and raising high income. Because, the production of these crops has characteristics requiring a lot of labor and it is difficult to produce horticultural crops at unheated plastic houses as the rural population ages. Therefore, we conducted a test to develop a crop planting system to cultivate crops in single span unheated plastic houses, although the utilization of labor is less than that of horticultural crops. The prior cropping cultivated three cultivars of sweet potatoes early, the second produced cultivated sweet potatoes, corn and soybeans. In the cultivation of the previous cropping, the sweet potatoes were harvested on the 113th day after planting on March 30th, the yield was 822 kg/10a for Pungwonmi, 1,377 kg/10a for Jinhongmi, 1,483 kg/10a for the Dahomi. Because of differences, the yield of Pungwonmi cultivar was less than that other cultivars and the yield of open field cultivations, we will expect further research. In the cultivation of the succeeding crops sweet potatoes were planted on July 27 and harvested 110 days later and investigated. The product yield of Pungwonmi cultivar was 1,024 kg/10a, and the Jinhongmi, Dahomi cultivars were not at economic level for sale and were necessary to review. In succeeding-crops, corn tested the Ilmichal cultivar, seeded on 27th July, harvested on October 11th. The day of silking was 45 days after sowing, the yield was 1,156 kg/10a, the goods rate was 100% level. The beans in the succeeding cultivation crop were sowed on 27th July, the early maturing of the varieties coming to Hwangeumol and Saeol cultivar, on 17th October, the late maturing soybean Daewonkong cultivar were harvested on October 21st. The yield of early maturing two cultivars was 214 kg/10a, Daewonkong was 257 kg/10a, and 100 seeds weight which were more than the early maturing beans were also heavy. When calculating these incomes price-wise according to the harvest time, we were able to consider the income in the order of corn, sweet potato and soybean from the second term crop. Various studies such as varieties, mulching method, moisture management, control environment management, etc. are considered necessary to develop cropping systems with sweet potato and field crops in future unheated plastic house.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.60 no.3
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• pp.282-292
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• 2015

Seeds of colored rice generally have high anthocyanin and flavonoid content but some cultivars have low seed germination rate. This defect poses unstable seedling establishment in nursery bed. The seed gravity variations of 10 colored rice varieties and its effect on germination, seed soaking duration and their optimum seeding rates for seedling raising in machine transplanting were investigated in the experiment. Based on seed gravity distribution of the 7 black- and 3 red-colored rice varieties at three levels of seed gravity (1.0<, 1.0~1.06, 1.06>), the black colored seeds such as Josaengheugchal and Sinmyungheugchal had high amount of specific gravity of below 1.0 ranging from 86~ 96%, while they had only 3~13% of seeds in specific gravity above 1.06. Sintoheugmi, Heugjinju, Heugnam, Heugkwang had various mass of seeds, showing 29~44% for specific gravity of below 1.0, 24~39% for 1.0~1.06 and 25~45% for above 1.06. On the other hand, the red colored rice such as Hongjinju, Jeogjinju and Geonkanghongmi had high percentage of specific gravity of above 1.06 with 84~86% while they had only 9~12% of specific gravity below 1.0 similar to Ilmibyeo of noncolored rice. The black colored seed generally showed low germination percentage, slow germination speed and long mean germination time, and low water absorption rate as compared with seed of the red colored rice which was similar to those of Ilmibyeo used as a control cultivar. The black colored seeds took 2~4 days longer seed soaking duration than the red colored rice before germination. This was related to high seed amount of specific gravity below 1.0 in black colored rices. The high amount of seed gravity above 1.06 in the colored rice seeds was positively correlated with percentage of germination, germination speed and mean germination time, ripened grain ratio and water absorption. Seed gravity distribution effect were less pronounced between back- and red- colored seeds in seedling emergence. The black colored rice has slightly lower normal seedling emergence rate than the red colored rice and Ilmibyeo in the seedbed soil due to high percentage of ungerminated seed. Normal seedling emergence rate of the black colored rice in the seedbed was 75.2~82.2% for 10-day old seedling and 85.3~ 90.9% for 30-day old seedling which was lower by 4.5~ 8.0% and 0~3.3%, respectively, than the red colored rice. Based on the normal seedling number per tray of Ilmibyeo for seeding rate of 10-day and 30-day old seedlings, the recommended seeding rate of black colored rice is 200~ 220g seeds for the 10-day old seedling and 110~130g for the 30-day old seedling in transplanting rice while the seeding rate of the red colored rice was 220g seeds for the 10-day old seedling and 130g for the 30-day old seedling.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.58 no.4
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• pp.432-438
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• 2013

This study was conducted to compare seed viability among 42 species after ten years of storage in the midterm storage complex ($4^{\circ}C$, 30-40% RH) at the National Agrobiodiversity Center (NAC) Korean genebank maintained by the Rural Development Administration (RDA), Republic of Korea and to suggest the relative seed longevity and suitable monitoring intervals. The germination data from initial tests and after ten years of storage were compared to measure changes in viability during storage. The decline in seed viability varied greatly among seeds from -11.5% for Triticum sp. to 80% for melon. Coriander, crowndaisy, safflower, cosmos, Chinesebellflower, waxgourd, melon, castorbean, Welch-onion, hollyhock, wild barley, and tallfescue showed significant decreases in viability of 34.2%, 73.4%, 36.5%, 30.0%, 40.2%, 71.3%, 80.0%, 65.9%, 45.5%, 51.4%, 53.0%, and 33.5%, respectively. Gardenpea, soybean, perilla, onion, wild rice, Italian-ryegrass, and pepper showed a 15-30% decline in viability, while the viability of morningglory, adzukibean, maize, and Capsicum sp. decreased by 15% to 5%. Chicory, radish, Chinese-cabbage, bottlegourd, watermelon, cucumber, pumpkin, Cucurbita sp., groundnut, kidneybean, clubwheat, sesame, wheat, Triticum sp., rice, barley, orchardgrass, buckwheat, and wild tomato showed changes in viability of <5%. The changes in storage viability also varied within families. The wild types of rice and barley showed rapid viability loss and presented different aspects from cultivars. Since seed viability of species, classified as index 1 or 2, showed germination losses >15% after ten years of storage, a viability test should be conducted with five year intervals, while species with germination loss of <15% (in index 3 or 4) can be retested at ten year intervals.

• Journal of Microbiology and Biotechnology
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• v.25 no.5
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• pp.569-578
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• 2015

Cold water paddy field soils are relatively unproductive, but can be ameliorated by supplementing with inorganic fertilizer from animal waste-based composts. The yield of two rice cultivars was significantly raised by providing either chicken manure or cow dung-based compost. The application of these composts raised the soil pH as well as both the total nitrogen and ammonium nitrogen content, which improved the soil's fertility and raised its nitrification potential. The composts had a measurable effect on the abundance of nitrogencycling-related soil microbes, as measured by estimating the copy number of various bacterial and archaeal genes using quantitative real-time PCR. The abundance of ammonia oxidizing archaea and bacteria was markedly encouraged by the application of chicken manure-based compost. Supplementation with the composts helped promote the availability of soil nitrogen in the cold water paddy field, thereby improving the soil's productivity and increasing the yield of the rice crop.

• Applied Biological Chemistry
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• v.36 no.6
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• pp.525-532
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• 1993

To characterize the prolamines in rice cultivars, the complete coding sequences of 17 prolamine genes from the database were analyzed. According to the phylogenic analysis of the sequences, these genes could be classified into 4 groups, Group I to IV. The multiple alignment of the deduced amino acid sequences revealed that the four groups differ from one another in chain length caused by deletion of short internal amino acids or carboxyl terminal fragments. Each group was also found to have different amino acid composition with 1, 4, 10 and 30% of sulfur containing amino acids (methionine and cysteine) in Group I to IV prolamines, respectively. Also the isoelectric points of these groups showed the different values of 9.2, 8.2, 6.7 and 7.4. Finally, from the analysis of codon usage pattern of prolamine genes, the codon usage for arginine, serine, threonine, isoleucine, asparagine, aspartic acid, glutamic acid and cysteine were higly biased. In the analysis of the codon usage pattern, the relation of the fraction of G/C ending codons to effective codon numbers suggests the different translational efficiency in the expression of the prolamine multigenes.

• Korean journal of applied entomology
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• v.24 no.1 s.62
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• pp.7-10
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• 1985

Transplanting date, planting space and nitrogen level were evaluated for disease development of rice sheath blight using two rice cultivars, Jinheung and Yushin. Sheath blight disease was more severe in early transplanting plot (May 16), narrow planting space($27{\times}12cm$) and high nitrogen level (220kg/ha for Jinheung and 300kg/ha for Yushin) conditions than in May 26 transplanting plot, wide planting space ($27{\times}15cm$) and standard nitrogen level (110kg/ha and l50kg/ha). At the same time, cultural conditions favorable for disease development during growth stage resulted in greater damage at maturing stage.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.35 no.6
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• pp.481-486
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• 1990

A survey was carried out on the cold-induced sterility of paddy rice in 1988 in the alpine area of Kangweon province when cold spell occurred during late July to early August, During this period minimum temperature as low as 8.5$^{\circ}C$ and 5.7$^{\circ}C$ was recorded at Dunnae and Jinbu, respectively. The rice cultivars, which encountered this cold spe]J at meiotic stage of microspore, were damaged by sterility in most alpine areas of higher than 300m in altitude. To secure spilkelet fertility higher than 80% it was estimated that the minimum and average air temperature accumulated during 13 days of meiotic stage should be higher than 2$25^{\circ}C$ and 285$^{\circ}C$, respectively and/or the duration of lower than 17$^{\circ}C$ in minimum temperature should not exceed fivedays during that period.