• Korean Journal of Agricultural Science
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• v.46 no.4
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• pp.827-841
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• 2019

The effect of genetically engineered rice Bt-9 on the diversity and abundance of plant-dwelling insects and spiders was tested under field conditions. Genetically engineered rice Bt-9, expressing mCry1Ac1 from Bacillus thuringiensis, confers resistance to rice leaf roller (Cnaphalocrocis medinalis) and provides tolerance to the herbicide glufosinate (PPT). The study compared Bt-9 and two non-GM reference varieties, Ilmi-byeo and Dongjin-byeo, at LMO isolated fields in Gunwi (Kyungpook National University) and Jeonju (National Institute Agricultural Sciences) in Southern Korea in 2016 - 2017. A total of 40,817 individuals from 62 families and 11 orders were collected from the two living modified organism (LMO) isolated fields. From the three types of rice fields, a total of 13,982, 14,105, and 12,730 individuals from the Bt-9, Ilmi-byeo and Dongjin-byeo were collected, respectively. Throughout the study, the analysis of variance indicated no significant differences (p < 0.05). Multivariate analysis showed that the abundance and diversity of plant dwelling insects were similar. The data on insect species population densities were subjected to principal component analysis (PCA), which did not distinguish among the three varieties, Bt-9 and the non-GM, reference cultivars, during the cultivation years. However, the results of the PCA analysis were completely divided into four groups based on the yearly survey areas. Therefore, there was no evidence for a negative impact of Bt-9 on the above-ground insects and spiders.

• Korean Journal of Food Science and Technology
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• v.28 no.5
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• pp.877-881
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• 1996

The firming rate of cooked rice stored at $4^{\circ},\;20^{\circ}$ and $30^{\circ}C$ were analyzed. The initial and limiting firmness of cooked rice were decreased as the moisture content increased from 57.5 to 69.5%. The firming rate was inversely related to the storage temperature, but was fairly constant at moisture content between $60.5{\sim}65.9%$. The activation energy and $Q_{10}$ value for firming of cooked rice was $-4.07{\times}10^3\;cal/mole$ and 1.26, respectively. No significant difference in firming rate was observed among rice cultivars.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.11
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• pp.6541-6548
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• 2013

Pigmented rice is mainly black, red, and dark purple, and contains a variety of flavones, tannin, polyphenols, sterols, tocopherols, ${\gamma}$-oryzanols, amino acids, and essential oils. The present study evaluated the cytotoxic effects of purple rice extracts (PREs) combined with chemotherapeutic drugs on human cancer cells and mechanisms of cell death. Methanolic (MeOH) and dichloromethane (DCM) extracts of three cultivars of purple rice in Thailand: Doisaket (DSK), Nan and Payao (PYO), were tested and compared with white rice (KK6). Cytotoxicity was determined by 3-(4, 5-dimethyl)-2, 5-diphenyltetrazolium bromide (MTT) assay in human hepatocellular carcinoma HepG2, prostate cancer LNCaP and murine normal fibroblast NIH3T3 cells. MeOH-PYO-PRE was the most cytotoxic and inhibited HepG2 cell growth more than that of LNCaP cells but was not toxic to NIH3T3 cells. When PREs were combined with paclitaxel or vinblastine, they showed additive cytotoxic effects on HepG2 and LNCaP cells, except for MeOH-PYO-PRE which showed synergistic effects on HepG2 cells when combined with vinblastine. MeOH-PYO-PRE plus vinblastine induced HepG2 cell apoptosis with loss of mitochondrial transmembrane potential (MTP) but no ROS production. MeOH-PYO-PRE-treated HepG2 cells underwent apoptosis via caspase-9 and-3 activation. The level of ${\gamma}$-oryzanol was highest in DCM-PYO-PRE (44.17 mg/g) whereas anthocyanin content was high in MeOH-PYO-PRE (5.80 mg/g). In conclusion, methanolic Payao purple rice extract was mostly toxic to human HepG2 cells and synergistically enhanced the cytotoxicity of vinblastine. Human HepG2 cell apoptosis induced by MeOH-PYO-PRE and vinblastine was mediated through a mitochondrial pathway.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.37 no.3
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• pp.299-303
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• 1992

Methods for harvesting and storage of rice were also originally introduced from the ancient China like other practices of agricultural techniques. Thus, optimum time or rice harvesting was adopted by appropriate draining to prevent the losses, and efficient tools were utilized for harvesting and manufacturing. In addition, our unique and original techniques for rice storage were learned and handed down, which prevented winds and rains, and allowed good ventilation. In the 15th and 16th centuries, treatment of smartweed extracts as emphasized to prevent insect damage for stored barley and rice cutting immediately at harvest was encouraged to prevent shattering of rice grains. In the 18th and 19th centuries, means for prevention of damages by water, insects and rats when grains stored in the field were suggested. Also, studies for ecology of various rice cultivars and storage techniques for a long period of time were necessarily emphasized to assume the optimum time of harvest regionally and to cooperatively work timely.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.12a
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• pp.64-64
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• 2020

Bakanae disease, caused by several Fusarium species, imposes serious limitations to the productivity of rice across the globe. The incidence of this disease has been shown to increase, particularly in major rice-growing countries. Thus, the use of high resistant rice cultivars offers a comparative advantage, such as being cost effective, and could be preferred to the use of fungicides. In this research, we used a tropical japonica rice variety, Zenith, a bakanae disease resistant line selected as donor parent. A RIL population (F_8:9) composed of 180 lines generated from a cross between Ilpum and Zenith was used. In primary mapping, a QTL was detected on the short arm of chromosome 1, covering about 3.5 Mb region flanked by RM1331 and RM3530 markers. The resistance QTL, qBK1^Z, explained about 30.93% of the total phenotype variation (PVE, logarith of the odds (LOD) of 13.43). Location of qBK1^Z was further narrowed down to 730 kb through fine mapping using additional RM markers, including those previously reported and developed by Sid markers. Furthermore, there is a growing need to improving resistance to bakanae disease and promoting breeding efficiency using MAS from qBK1^Z region. The new QTL, qBK1^Z, developed by the current study is expected to be used as foundation to promoting breeding efficiency with an enhanced resistance against bakanae disease. Moreover, this study provides useful information for developing resistant rice lines carrying single or multiple major QTLs using gene pyramiding approach and marker-assisted breeding.

• Research in Plant Disease
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• v.25 no.4
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• pp.196-204
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• 2019

Rice panicle blast occurred severely in southern provinces of Korea in 2014. The proportion of panicle blast incidence area to cultivated area of rice were 11.0% and 14.6% in Jeollanam-do and Gyeongsangnam-do, respectively. To identify the causal factors of the outbreak, we investigated weather conditions in August, amount of cultivated area of mainly grown cultivars, and nitrogen contents in plants with different disease incidences in 2014. 'Saenuri,' 'Ilmibyeo,' 'Unkwang,' 'Dongjin 1 ho,' 'Nampyeongbyeo,' and 'Hwangkeumnuri' were mainly grown cultivars. Monthly average of daily air temperature in August 2014 was 3.2℃ and 3.1℃ less than 2018 in Haenam and Miryang, respectively. Rainfall in August 2014 was 70.0% and 42.0% greater than 2018 in Haenam and Miryang, respectively. The numbers of blast warning days in August calculated nationwide using a forecast model for blast infection were higher in 2014 than in 2018, and they were in high level throughout the country in 2014. Nitrogen contents in plant samples from high-incidence plots were significantly higher than those from low-incidence plots. Consequently, excessive use of nitrogen fertilizers was the main factor for the disease outbreak at the level of specific farms, in addition to the collective cultivation of susceptible cultivar, low temperatures and frequent rainfalls in August.

• Korean Journal of Food Science and Technology
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• v.50 no.5
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• pp.492-498
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• 2018

The current study investigated the effect of rice cultivars (Samkwang and Seolgaeng) and milling methods on various characteristics of rice bread. In dry milling, all component characteristics, except the water solubility index, were higher. In terms of the morphology of starch, Samkwang exhibited an angular shape, while Seolgaeng exhibited a round shape. Wet milled flour yielded fine polygonal starch compared to dry milled flour. In regard to the quality characteristics of bread, both wet milled Samkwang and dry milled Seolgaeng showed closely similar specific volumes of 2.90 mL/g and 2.86 mL/g, respectively. Hardness was in the range of 555.50 - 597.29 g for Seolgaeng and 776.78 - 823.06 g for Samkwang. Thus, although the flour characteristics were different, dry milled Seolgaeng, the rice flour of which is economical to produce and has baking characteristics similar to that of wheat flour, appears to be preferable.

• Journal of Wetlands Research
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• v.16 no.4
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• pp.363-370
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• 2014

We grew seedlings of Saechucheongbyeo and Odaebyeo of rice cultivars that are cultivated dominantly in the northern and middle regions of Korea under control(ambient condition), ambient $CO_2$ concentration+elevated temperature, and elevated $CO_2$ concentration+elevated temperature in order to study how growth responses and crop yield of major rice of Korea change as the global warming proceeds and compared the results. Aboveground biomass, belowground biomass, total biomass, and panicles weight per individual and ripended grain rate of cv. Saechucheongbyeo were the highest under control, but those of cv. Odaebyeo were the highest under elevated $CO_2$ concentration+elevated temperature. There was no difference in the number of panicles per individual of cv. Saechucheongbyeo and cv. Odaebyeo in these experiments. There was no difference in the number of grains per panicle of cv. Saechucheongbyeo among three environmental gradients, but that of cv. Odaebyeo was the highest under elevated $CO_2$ concentration+elevated temperature. Weight of a grain of cv. Saechucheongbyeo was highest under elevated $CO_2$ concentration+elevated temperature, but that of cv. Odaebyeo was the higher under ambient $CO_2$ concentration+elevated temperature and elevated $CO_2$ concentration+elevated temperature. Thus, if global warming continues in Korea, selection of rice cultivation varieties must be chosen carefully for commendation.

• Applied Biological Chemistry
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• v.25 no.3
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• pp.135-141
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• 1982

Two rice cultivars, Jinju and Iri 348, were used to compare the changes in the contents of some organic metabolites and ions and in some enzyme levels under water-and salt-stressed conditions. The water loss and proline accumulation under water and salt stresses were accelerated more in the salt-sensitive cultivar Iri 348 than in the salt-tolerant Jinju. The contents of crude protein, total free amino acids, proline and polyphenols increased under water-or salt-stressed rice, but that of reducing sugar increased under water stress only. The water-and salt-stresses induced the high ratio of low molecular organic solutes to crude protein in Jinju but not in Iri 348. The ratio of total free amino acids to crude protein increased under the stressed conditions was likely due to high protease activity. The contents of $Na^+$ and $Cl^-$ were higher in Iri 348 than in Jinju. Iri 348 had higher values of $Na^+/Ca^{2+}$ and monovalent/divalent of cations, but lower of $K^+/Na^+$ than Jinju Rice. The further studies should emphasize to set the correlations between these ratios and tolerance to water and salt stresses among rice cultivars.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.53 no.4
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• pp.440-446
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• 2008

Successful germination and establishment of seedlings in flooded paddy are critical in direct seeding cultivation of rice. In this study, we examined the relationship between antioxidant enzymes and alcohol dehydrogenase (ADH) activities and coleoptile elongation under submergence of deep water with two rice cultivars, Iksan429 and Woodrose, which show characteristic coleoptile elongation under hypoxic condition. The growth of shoot under submerged in water was faster than the root. The survival duration was longer in Iksan429 than in Woodrose under submerged in water. The alcohol dehydrogenase (ADH) activities were significantly increased under hypoxia compared to in aerated condition. The ADH activity was increased in Iksan429 more than in Woodrose under hypoxia. The superoxide dismutase (SOD) activity in Iksan429 was gradually increased up to 5 days after treatment (DAT) then decreased slowly till 14 DAT under water, whereas in Woodrose it was dramatically decreased after 5 DAT. The peroxidase (POX) activity in Iksan429 was significantly increased until 7 DAT under hypoxia, whereas it was not significantly different in Woodrose during hypoxic treatment. However, in non-treated condition, POX activity in Woodrose was increased more than Iksan429. The changes of catalase (CAT) activities showed no differences in both cultivars. We suggest that the overexpression of ADH, SOD and POX activities is responsible for the hypoxic tolerance and plays an important role in the surviving of rice seedling.