• The Korean Journal of Pesticide Science
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• 제14권2호
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• pp.95-103
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• 2010

Fomesafen is a selective herbicide, and used to control annual and perennial broad-leaf grass on soybean and fruit fields in USA and China, but not introduced in Korea yet. So, MRL (Maximum Residue Level), and analytical method of fomesafen were not establishment in Korea. Therefore, this experiment was conducted to establish a determination method for fomesafen residue in crops using HPLC-UVD/MS. Fomesafen residue was extracted with acetone from representative samples of five raw products which comprised hulled rice, soybean, apple, green pepper, and Chinese cabbage. The extract was diluted with saline water, and dichloromethane partition was followed to recover fomesafen from the aqueous phase. Florisil column chromatography was additionally employed for final clean up of the extract. The fomesafen was quantitated by HPLC with UVD, using a Shiseido CAPCELL-PAK UG C18 column. The crops were fortified with fomesafen at 3 levels per crop. Mean recovery ratio were ranged from 87.5% for a 0.4 ppm in hulled rice to 102.5% for a 0.4 ppm in apple. The coefficients of variation were ranged from 0.6% for a 2.0 ppm in hulled rice to 7.7% for a 0.04 ppm in green pepper. Quantitative limit of fomesafen was 0.04 mg/kg in representative 5 crop samples. A LC/MS with selected-ion monitoring was also provided to confirm the suspected residue. Therefore, this analytical method was reproducible and sensitive enough to determine the residue of fomesafen in agricultural commodities.

• Journal of Mushroom
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• 제12권2호
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• pp.117-121
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• 2014

The experiments were conducted to provide information on the chemical concentrations and cultural characteristics in the periods of hyphal incubation, primordial formation, and fruiting bodies yield of winter mushroom, Flammulina velutipes at the mixture ratio of the raw materials. Substrates were analyzed for pH, total carbon (T-C), total nitrogen (T-N), and C/N ratio. In case of Flammulina velutipes, yield of fruiting bodies were 190.5 g/850 ml in the substrates, [Corn-cob + Rice bran + Soybean curd residue (75:20:5)], which was increased 20.4% more than the control, [Douglas fir sawdust + Rice bran (75:25)]. But the periods of hyphal incubation took 28 days, which was 7 days longer than the control. Also, in the substrates [Douglas fir sawdust + Rice bran + Soybean curd residue (75:20:5)], the yields was 172.7 g/850 ml and the periods of hyphal incubation was 21 days.

• Korean Journal of Food Science and Technology
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• 제13권1호
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• pp.20-24
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• 1981

Residues of neoasozine in rice grain were determined by neutron activation and colorimetric techniques. Twice application of the chemical before flowering did not lead to any increased residue level while 4-times application resulted in significant increase in the residue level up to 0.54-0.75 mg $As_2O_3/kg$. The partition ratio of arsenic residues into polished rice grain and bran was 73 : 27 in 100% polishing while most of the residues in the bran was transferred to oil cake fraction during solvent extraction, reaching up to 2.9 mg $As_2O_3/kg$. The neutron activation technique was advantageous because of its high sensitivity and the smaller sample amounts required for analysis.

• Korean Journal of Food Science and Technology
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• 제19권6호
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• pp.475-479
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• 1987

This survey was conducted to Investigate the NaCl contents of meals taken from common restaurants in Seoul area and further to estimate the total dietary intake of salt from the meals. The samples included Seoleung-tang (beef and rice soup)/Galbitang (beef-rib soup), Yeukkye-jang (spicy beef soup), Doenjangchigye-bab (boiled rice with soy paste stew), Bibim-bab (boiled rice with assorted mixtures) and Bibim-naengmyon (buck wheat vermicelli with assorted mixtures). The average content of NaCl for each sample was determined by saltmeter and its daily intake level was estimated. Doenjangchigye-bab was shown the highest concentration of NaCl Doen-jangchigye-bab was separated into drained residue and fluid, and the average NaCl content in the fluid was higher than that in the residue. In comparision of northern and southern region of Seoul divided by Han river, there was no significant difference in the content of NaCl. The average content of NaCl per meal was 10.6g, and from these data, the daily dietary intake of salt for adults was estimated to be 28.5g.

• KOREAN JOURNAL OF CROP SCIENCE
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• 제47권2호
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• pp.132-136
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• 2002

The rice straw managements are essential for maintaining soil fertility as well as reducing chemical fertilizer application in paddy field. A field experiment was conducted on moderately well draining alluvial paddy soil to investigate the decomposition pattern of rice straw. The mesh bags containing the rice straw harvested in the previous year were placed at soil surface and buried into around 10cm depth and recovered periodically for determining the straw decomposition. Pot experiments were conducted to investigate the fates of N released from $^{15}$ N-labeled rice straw under different levels of N fertilizer application. The overall decomposition patterns of rice straw were similar for the two incorporation depths in transplanted paddy field. The straw incorporated at transplanting date showed weight loss of about 50%, 70% and 90% after 2 months, 5 months, and 2 years, respectively. The decompositions of straw cell wall components showed somewhat different pattern. The decompositions of cellulose and silica were similar to that of dry weight while the decomposition of lignin was slower than that of cellulose and silica. N was released from rice straw 42% and 65 % of the initial N after one month and after five months, respectively. P release was faster than N release. Recoveries of rice straw-$^{15}$ N by rice plants were 10.2, 13.4 and 14.9% in 0, 120 and 240 mg N pot$^{-1}$ , respectively. Soil recoveries of rice straw $^{15}$ N were 17.3, 20.6 and 18.9% in 0, 120 and 240mg N pot$^{-1}$ , respectively.

• The Korean Journal of Food And Nutrition
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• 제10권2호
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• pp.180-185
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• 1997

Sikye was produced from glutinous rice. The glutinous rice Sikhye was found to contain 7.3% of limit dextrin, 10.1% of maltose, 1.3% of maltotriose and 1.75% of rice residue. Limit dextrin in glutinous rice Sikhye was purified by ethanol fractionation followed by gel chromatography on Biogel P-2. The purified limit dextrin showed both signal of $\alpha$-1,4- and $\alpha$-1,6-glucosidic linkage with its estimation ratio of 5:1 by 1H-NMR analysis. Limit dextrin was digested with enzymes(30units/ml) of $\alpha$-amylase, $\alpha$-glucosidase and glucoamylase from Aspergillus awamori, sweet potato $\beta$-amylase and human salivary $\alpha$-amylase at 37$^{\circ}C$ for 1 hour, respectively. Hydrolysis rates of these amylases on it were similar that of rice Sikhye. $\alpha$-Glucosidase plus human salivary $\alpha$-amylase hydrolyzed it to 18%. The results suggest that glutinous rice is more effective to produce high level of branched maltooligosaccharide compared with rice as raw material for Sikye making.

• Applied Biological Chemistry
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• 제32권2호
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• pp.98-103
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• 1989

For the effective use of soymilk residue (SR), by-product of soymilk production, rice Doenjang was manufactured. As the results, the contents of reducing sugar were increased till $40{\sim}50days$ in all samples and decreased thereafter during aging of the mash. The contents of amino nitrogen were prominently increased till 50days, but increasing ratio was slightly slowdowned thereafter. The higher mixing ratio of SR, the less were the contents of reducing sugar and amino nitrogen. The contents of total acid were also prominently increased till 30days, but were slowdowned thereafter and the differences among all treatments were small. As the mixing ratio of SR was increased, the lightness and the yellowness were increased and the redness was decreased in 80days aged samples. In sensory evaluation of 90days aged samples, control was the best in soy sprout Doenjang soup. As the mixing ratio of SR was increased, the taste was dropped slightly but no significant differences were obtained between control and sample A (rice koji 100, soybean 75, SR 25), and among A,B(rice koji 100, soybean 50, SR 50). C(rice koji 100, soybean 25, SR 75) and D(rice koji 100, soybean 0, SR100). In the original taste of Doenjang, the control was the best and as the mixing ratio of SR was increased, the point was dropped but there were no significant differences between control and A, and among A, B and C, and among B, C and D.

• BMB Reports
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• 제38권5호
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• pp.595-601
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• 2005

In this study, we have isolated a rice (Oryza sativa L.) glutamate decarboxylase (RicGAD) clone from a root cDNA library, using a partial Arabidopsis thaliana GAD gene as a probe. The rice root cDNA library was constructed with mRNA, which had been derived from the roots of rice seedlings subjected to phosphorus deprivation. Nucleotide sequence analysis indicated that the RicGAD clone was 1,712 bp long, and harbors a complete open reading frame of 505 amino acids. The 505 amino acid sequence deduced from this RicGAD clone exhibited 67.7% and 61.9% identity with OsGAD1 (AB056060) and OsGAD2 (AB056061) in the database, respectively. The 505 amino acid sequence also exhibited 62.9, 64.1, and 64.2% identity to Arabidopsis GAD (U9937), Nicotiana tabacum GAD (AF020425), and Petunia hybrida GAD (L16797), respectively. The RicGAD was found to possess a highly conserved tryptophan residue, but lacks the lysine cluster at the C-proximal position, as well as other stretches of positively charged residues. The GAD sequence was expressed heterologously using the high copy number plasmid, pVUCH. Our activation analysis revealed that the maximal activation of the RicGAD occurred in the presence of both $Ca^{2+}$ and calmodulin. The GAD-encoded 56~58 kDa protein was identified via Western blot analysis, using an anti-GAD monoclonal antibody. The results of our RT-PCR analyses revealed that RicGAD is expressed predominantly in rice roots obtained from rice seedlings grown under phosphorus deprivation conditions, and in non-germinated brown rice, which is known to have a limited phosphorus bioavailability. These results indicate that RicGAD is a $Ca^{2+}$/calmodulin-dependent enzyme, and that RicGAD is expressed primarily under phosphate deprivation conditions.

• Korean Journal of Food Science and Technology
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• 제18권4호
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• pp.264-269
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• 1986

This study was undertaken to investigate the removal efficiency of heavy metals (Cd, Pb) in rice and wheat flour by cooking and processing. After four times washing of rice grains with water, the removal ratios of cadmium and lead from artificially-contaminated samples were 39.271 and 34.9%, respectively. In cookingof rice grains washed three-times with water, the residue levels of cadmium and lead decreased to 85.7%, 83.0% in electric rice cooker and 74.51%, 72.6% in pressure rice cooker, respectively. No significant difference was observed in the removal of the metals between naturally-contaminated and artificially contaminated rice samples, and between the electric and pressure rice cookers. In baking of artificially-contaminated wheat flour into cookie and loaf bread, the removal ratios of cadmium and lead were negligible as expected. When knife-cut noodle was made from artificially-contaminated wheat flour and boiled in water, 75% of the metals were found in the noodle solids and only 25% of them were removed into the broth.

• Korean journal of applied entomology
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• 제40권1호
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• pp.83-88
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• 2001

Residual effect of imidacloprid on brown planthopper (BPH) and residues on rice plant were investigated in pot and field tests. Residual effect (showing 100% mortality) of imidacloprid of granule formulation on the BPH lasted for 40 days at a recommended dose (0.3 kg a.i./ha) and for 30 and 20 days at a half and a quarter of the recommended dose, respectively. In case of liquid formulation of imidacloprid, the residual effect lasted for 40 days at both recommended dose (0.032 kg a.i.1ha) and a half of it, and lasted for 30 days at a quarter of it. The next generation formation of BPH was throughly depressed by the imidacloprid application at the former generation. This result supports the residual effect of imidacloprid on BPH. Seed dressing (3 ghg) showed insecticidal activity (96.7% mortality) for 55 days after treatment. When granule formulation of imidacloprid was applied to soil at a recommended dose , imidacloprid residue in leaves of rice plant increased up to 0.46 ppd for 6 days and then decreased to 0.09 ppm on 40 days after application. Residue of imidacloprid in leaves of rice plant increased up to about 0.23 ppm and about 0.2 ppm for 6 days when applied at a half and a quarter of the recommended dose, respectively and after that they decreased.