• 식물조직배양학회지
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• 제28권2호
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• pp.97-101
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• 2001

동화산물의 분배 효율 개선을 통한 생산성 향상 가능성을 조사하고자 감자의 sucrose 수송자 유전자를 벼에 형질전환 하였다. 동진벼로부터 유도된 callus를 감자의 sucrose 수송자 유전자가 도입된 A. tumefaciens LBA 4404와 공동배양한 후, 선발배지에서 증식된 callus를 250 mg/L carbenicillin, 2 mg/L kinetin, 0.1 mg/L NAA가 첨가된 MS 배지에 옮겨 약2주 후부터 소식물체를 얻었다. Carbenicillin 250 mg/L 첨가된 MS 기본 배지에서 소식물체의 발근을 유도하여 재분화 식물체를 얻었다. 선발된 callus는 약 150%의 높은 식물체 재분화율을 보였다. 재분화 식물체에 대한 PCR 분석을 수행하여 감자의 sucrose수송자 유전자가 삽입된 형질전환 벼 식물체를 선발하였다. 선발된 형질전환 식물체에 대한 Southern blot 분석을 통하여 외래유전자인 감질 sucrose 수송자 유전자가 벼 genome 내에 안정적으로 도입되었음을 확인하였다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.107-107
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• 2022

The increase in atmospheric temperature due to climate change prolongs the period of exposure to high-temperature environments during rice cultivation. In particular, high-temperature during early seed development greatly affects on the productivity and quality of rice. The high temperature at this time not only affects the transport and distribution of assimilates from leaves to seeds and the accumulation of starch in the seeds, but also affects the leaves, which are the production organs of assimilates, and increases the consumption of assimilation products due to an increase in respiration. Therefore, in this study, rice was grown in temperature gradient chambers(TGC) to analyze the effects of high temperature on physiological responses, assimilate production, and changes in gene expression in rice leaves. Analysis of chlorophyll and sugar contents and RNA-seq experiments were performed using flag leaves collected under normal and elevated temperature conditions, respectively, during the early seed development stage, and then these results were comprehensively discussed.

• 한국축산시설환경학회지
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• 제13권2호
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• pp.139-146
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• 2007

본 연구는 가축액상분뇨의 비료성분 불균형을 화학비료로 조정하여 작물양분의 최적화에 의한 벼의 생육과 품질향상을 검토하기 위하여 수행되었다. 양돈분뇨발효액비와 화학비료를 혼합하여 조제한 맞춤비료(LCSC)의 시용이 벼의 생육특성, 수량, 수량구성요소, 쌀 품질에 미치는 영향을 조사하였다. 처리구는 맞춤비료(LCSC) 기비 50% + 화학비료 50% 추비구, LCSC 기비 75% + 화학비료 25% 시용구, 100% LCSC 시용구, 100% 양돈 분뇨발효액비 시용구(LM)를 두었다. 또한 대조구로 화학비료 시용구를 두었다. 1. 초장은 생육초기에 화학비료 시용구와 LCSC와 화학비료 혼합 시용구에서 가장 큰 것으로 나타났다. 생육후기의 초장은 LM 시용구가 가장 적었다. 분얼수의 경우도 화학비료 시용구와 LCSC 시용구가 LM 시용구 보다 더 많았다. 엽록소 함량은 생육초기 화학비료 및 LCSC 시용구에서 LM 시용구 보다 높았으나 생육후기에는 LM 처리구와 LCSC 100% 처리구 사이에 유의한 차이가 없었다. 2. 수량구성요소에 있어서 주당수수는 화학비료구와 LCSC 기비 + 화학비료 추비 시용구에서 가장 많은 것으로 나타났다. 수당립수는 화학비료 시용구에서 가장 많았으나 처리간에 유의한 차이를 나타내지 않았다. 천립중과 등숙률은 처리구 사이에 유의한 차이를 나타내지 않았다. 3. 벼의 정조수량은 LCSC + 화학비료 혼합 시용구에서 681 kg/10a로서 가장 높았다. LCSC 100% 기비시용구의 정조수량은 화학비료 대비 9% 감수되었으며, LM 시용구는 화학비료 시용구 대비 7% 감수되었다. 4. 현미의 미질은 LM 시용구와 LCSC 100% 기비 시용구에서 화학비료 시용구에 비하여 유의성 있게 높게 나타났다. 현미의 단백질 함량은 화학비료 시용구에서 LM 시용구와 LCSC 100% 기비 시용구보다 유의성 있게 높았다. 따라서 쌀 품질향상에 적절한 양의 LCSC와 화학비료 혼합시용이 효과가 있는 것으로 나타났다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.311-311
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• 2017

The relationships between rice grain filling and air temperature of maturing period in paddy field were analyzed to evaluate the effects of climatic change on rice productivity in Korea. Data of air temperature of 31, 41 and 51 days after heading(DAH) for 11 years from 2002 to 2016 were collected and analyzed to determine the effects on rice yield and yield component related traits of Chucheogbyeo, popular cultivar in Gyeonggi province in Korea. As the results, ripening ratio was closely correlated with the mean of daily maximum temperature (DMAT $r=0.71^*$), the mean of daily temperature difference (DTD, $r=0.67^*$) of 41 DAH and DTD ($0.65^*$) of 51 DAH. Weight of 1,000 paddy rice grains was closely correlated with accumulated sunshine hours (ASH) of 31 ($r=0.84^{**}$), 41 ($r=0.75^{**}$), 51 ($r=0.72^*$) DAH. Brown rice grain weight recovery ratio was closely correlated with DTD ($r=0.76^{**}$) and ASH ($r=0.84^{**}$) of 31 DAH, DMAT ($r=0.75^{**}$, $r=0.79^{**}$), DTD ($r=0.79^{**}$, $r=0.77^{**}$) and ASH ($r=0.81^{**}$, $r=0.79^{**}$) of 41 and 51 DAH. Paddy rice yield was closely correlated with MDT ($r=-0.63^*$) of 31 DAH, mean of daily minimum temperature (DMIT, $r=-0.83^{**}$, $r=-0.70^*$), DTD ($r=0.71^*$, $r=0.62^*$) of 31 and 41 DAH. Brown rice yield was correlated closely with DMIT ($r=-0.86^{**}$, $r=-0.73^*$) and DTD ($r=0.80^{**}$, $r=0.72^*$) of 31, 41 DAH, and DTD ($r=0.69^*$) of 51 DAH. Milled rice yield was correlated closely with DMIT ($r=-86^{**}$, $r=-0.73^*$), DTD ($r=0.79^{**}$, $r=0.71^*$) of 31, 41 DAH, and DTD ($r=0.68^*$) of 51 DAH.

• 한국식품영양학회지
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• 제27권3호
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• pp.414-420
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• 2014

This study was performed to increase the yield and to reduce the processing times for the preparation to improve the productivity and quality of rice jochung, a traditional food in Korea. In order to evaluate the quality characteristics and yield of jochung, the viscosity, color value, mineral contents and the sensory evaluation were measured. Jochung is prepared from steamed rice (STR), wet-milled rice flour (WRF) and dry-milled rice flour (DRF) by processing methods of rice and reacting times (6 hours or 13 hours) of liquefaction and saccharification. There is commonly added liquefying enzyme for rice liquefaction (0.4%/10 kg rice, at $85{\sim}90^{\circ}C$ for 1 hour or 4 hours) and saccharogenic enzyme with malt (2.5% or 4.5%/10 kg rice, at $56{\sim}60^{\circ}C$ for 5 hours or 9 hours). The inner structural properties of WRF showed the more distinct shape regular structure of uncombined starch particles but the DRF closely maintained particles of rice flour observed by SEM. If processing times for liquefation and saccharification were reduced from 13 hours to 6 hours, the yield of jochungs prepared with WRF increased 8%, the DRF 7%, and the STR 3% respectively and the sensory evaluation as well as color values and overall desirability received high scores. The viscosity, color a and b values of jochung processed with WRF for 6 hours were lower than that processed for 13 hours. The viscosity and color a, b value and Ca content were decreased in the jochung processed with WRF or DRF for 6 hours, but Mg, P and K were increased than that of STR. Jochung processed by 0.4% liquefying enzyme and 2.5% malt with WRF for 6 hours will increase the yield, save manufacturing times and costs and will thereby enable cost-effective techniques.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.57-57
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• 2022

In many areas of the world, salt damage and drought have had a negative impact on human survival due to a decrease in agricultural productivity. For instance, about 50% of agricultural land will be affected by salt damage by 2050. Biostimulants such as plant extracts can not only increase the nutrient utilization efficiency of plants, but also promote plant growth and increase resistance to abiotic or biotic stress. Therefore, the objective of this study was to determine how selected plant extracts might reduce levels of stress caused by drought and salt and to better understand the physiological response mechanisms of rice plants. In this study, we used Soybean leaves, Soybean stems and Allium tuberosum, Allium cepa, Hizikia fusiforme, and Gracilaria verrucosa extracts were used. These extracts had been used in previous studies and were found to be effective. The materials were dried in a dry oven at 50℃ for 5 days and ground using a blender. Each 50 g of materials was put in 1 L of distilled water, stirred for 24 hours, filtered using 4 layers of mirocloth, and then concentrated using a concentrator. Rice (cv. Hopumbyeo) seeds were immersed and germinated, and then sown in seedbeds filled with commercial soil. In drought experiments, three rice seedlings at 1 week after seeding was transplanted into 100 ml cups filled with commercial soils and grown until the 4-leaf stage. For this experiment, the soil weight in a cup was equalized, and water was allowed to become 100% saturated and then drained for 24 hours. Thereafter, plant extracts at 3% concentrations were applied to the soils. For NaCl treatments, rice plants at 17 days after seeding were treated with either 100 mM NaCl or plant extracts at 1%+ 100 mM NaCl combinations in the growth chamber. Leaf injury, relative water content, photosynthetic efficiency, and chlorophyll contents were measured at 3, 5, and 6 days after treatments. Shoot fresh weight of rice under drought conditions increased 28-37% in response to treatments of Soybean leaf, Soybean stem, Allium tuberosum, Allium cepa, Hizikia fusiforme, and Gracilaria verrucosa extracts at 3% when compared with control plants. Shoot fresh weight of rice subjected to 100 mM NaCl treatments also increased by 6-24% in response to Soybean leaf, Soybean stem, Allium tuberosum, Allium cepa, Hizikia fusiforme, and Gracilaria verrucosa extracts at 3% when compared with control plants. Compared to the control, rice plants treated with these six extracts and subjected to drought conditions had significantly higher relative water content, Fv/Fm, total chlorophyll and total carotenoids than control plants. With the exception of relative water contents, rice plants treated with the six extracts and subjected to salt stress (100 mM NaCl treatments) had significantly higher Fv/Fm, total chlorophyll and total carotenoids than control plants. However, the type of extract used did not produce significant difference in these parameters. Thus, all the plant extracts used in this study could mitigate drought and NaCl stresses and could also contribute substantially to sustainable crop production.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.361-361
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• 2017

The NPK are known as macro elements that affect crop growth and yield. In 1989, Cambodia Agricultural Research and Development Institute (CARDI) gave a recommendation rate of fertilizer on rice production based on soil types. This recommended rate of NPK seems however relatively low as compared to farmers' practices nowadays and the amount in the neighboring countries. The CARDI recommended rate for Prateah Lang soil type is 50kg N, $25kg\;P_2O_5$, $25kg\;K_2O\;ha^{-1}$ while recent farmers' practice rates are 55 - 64kg N, 24 - 46kg $P_2O_5$, $30kg\;K_2O\;ha^{-1}$. However, the overuse of chemical fertilizer will lead to un-preferable plant growth, insect pest, disease and economic yield. Thus, we examined the effect of different NPK application rates on the growth and yield components in Prateah Lang soil type in Takeo province to investigate appropriate rates for improving rice productivity with economic efficiency. This study was conducted from July to November during wet season in 2013. A multi-locational trial with 6 treatments (T0 - T5) of NPK rates in 5 locations (trial 1 - 5) with 3 replications was conducted. The different combinations of NPK application were employed from 0, 50, 60, 80, 100, $120kg\;N\;ha^{-1}$, 0, 25, 30 45, $60kg\;P_2O_5\;ha^{-1}$ and 0, 15, 25, 30, $45kg\;K_2O\;ha^{-1}$. Urea, DAP and KCl were used for fertilization. Split application was employed [basal: 20% of N, 100% of P and K, top dressing-1st: 40% of N (30DAT), 2nd: 40% of N (PI stage)]. Three-week-old seedlings of var. Phka Rumdoul were transplanted with 2 - 3 seedlings $hill^{-1}$ with $20cm{\times}20cm$ spacing. Plant length, tiller number at the maximum tillering stage and yield components were measured. The different rates of NPK application affected some yield components. The panicle number per hill was the most important key component followed by the spikelet number per panicle. However, the other parameters such as the filled grain percentage and 1000 grains weight had small effect or weak relation with the yield. Although the panicle number per hill had a significantly positive correlation with the stem number per hill, it was not correlated with the percentage of productive culms. The variation in the grain yield among the 5 trials was small and the difference was not significant. Although the yield tended to be higher at higher N and P application, there was no significant difference above 60kg N and $30kg\;P_2O_5$. The yield was the highest at 15, 30 and $45kg\;K_2O$ followed by $25kg\;K_2O$. The relationships between N, P and the stem number per hill were significantly linear positive, though it was not linear between K and the stem number. From these results, to increase rice productivity in the target area, farmers' effort to increase N and P input rather than CARDI recommendation up to 60kg N and $30kg\;P_2O_5$ will be sufficient considering economic efficiency. Besides, the amount of K application should be reconsidered.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.363-363
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• 2017

The NPK are known as macro elements that affect crop growth and yield. In 1989, Cambodia Agricultural Research and Development Institute (CARDI) gave a recommendation rate of fertilizer on rice production based on soil types. This recommended rate of NPK seems however relatively low as compared to farmers' practices nowadays and the amount in the neighboring countries. The CARDI recommended rate for Prateah Lang soil type is 50kg N, 25kg P2O5, 25kg K2O ha-1 while recent farmers' practice rates are 55 - 64kg N, 24 - 46kg P2O5, 30kg K2O ha-1. However, the overuse of chemical fertilizer will lead to un-preferable plant growth, insect pest, disease and economic yield. Thus, we examined the effect of different NPK application rates on the growth and yield components in Prateah Lang soil type in Takeo province to investigate appropriate rates for improving rice productivity with economic efficiency. This study was conducted from July to November during wet season in 2013. A multi-locational trial with 6 treatments (T0 - T5) of NPK rates in 5 locations (trial 1 - 5) with 3 replications was conducted. The different combinations of NPK application were employed from 0, 50, 60, 80, 100, 120kg N ha-1, 0, 25, 30 45, 60kg P2O5 ha-1 and 0, 15, 25, 30, 45kg K2O ha-1. Urea, DAP and KCl were used for fertilization. Split application was employed [basal: 20% of N, 100% of P and K, top dressing-1st: 40% of N (30DAT), 2nd: 40% of N (PI stage)]. Three-week-old seedlings of var. Phka Rumdoul were transplanted with 2 - 3 seedlings hill-1 with $20cm{\times}20cm$ spacing. Plant length, tiller number at the maximum tillering stage and yield components were measured. The different rates of NPK application affected some yield components. The panicle number per hill was the most important key component followed by the spikelet number per panicle. However, the other parameters such as the filled grain percentage and 1000 grains weight had small effect or weak relation with the yield. Although the panicle number per hill had a significantly positive correlation with the stem number per hill, it was not correlated with the percentage of productive culms. The variation in the grain yield among the 5 trials was small and the difference was not significant. Although the yield tended to be higher at higher N and P application, there was no significant difference above 60kg N and 30kg P2O5. The yield was the highest at 15, 30 and 45kg K2O followed by 25kg K2O. The relationships between N, P and the stem number per hill were significantly linear positive, though it was not linear between K and the stem number. From these results, to increase rice productivity in the target area, farmers' effort to increase N and P input rather than CARDI recommendation up to 60kg N and 30kg P2O5 will be sufficient considering economic efficiency. Besides, the amount of K application should be reconsidered.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 1999년도 Proceedings of International Symposium on Remote Sensing
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• pp.107-114
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• 1999

The large-scale distribution of crops Is usually determined by climate. We present the results of a climate-crop prediction based on spatial bio-physical process model approach, implemented in a GIS (Geographic Information System) environment using several regional and global agriculture-environmental databases. The model utilizes daily climate data like temperature, rainfall, solar radiation being generated stocastically by in-built model weather generator to determine the daily biomass and finally the crop yield. Crops are characterized by their specific growing period requirements, photosynthesis, respiration properties and harvesting index properties. Temperature and radiation during the growing period controls the development of each crop. The model simulates geographic/spatial distribution of climate by which a crop-growing belt can also be determined. The model takes both irrigated and non-irrigated area crop productivity into account and the potential increase in productivity by the technical means like mechanization is not considered. All the management input given at the base year 1995 was kept same for the next twenty-year changes until 2015. The simulated distributions of crops under current climatic conditions coincide largely with the current agricultural or specific crop growing regions. Simulation with assumed weather generated derived climate change scenario illustrate changes in the agricultural potential. There are large regional differences in the response across the country. The north-south and east-west regions responded differently with projected climate changes with increased and decreased productivity depending upon the crops and scenarios separately. When water was limiting or facilitating as non-irrigated and irrigated area crop-production effects of temperature rise and higher $CO_2$ levels were different depending on the crops and accordingly their production. Rise in temperature led to yield reduction in case of maize and rice whereas a gain was observed for wheat crop, doubled $CO_2$ concentration enhanced yield for all crops and their several combinations behaved differently with increase or decrease in yields. Finally, with this spatial modeling approach we succeeded in quantifying the crop productivity which may bring regional disparities under the different climatic scenarios where one region may become better off and the other may go worse off.

• 한국미생물·생명공학회지
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• 제43권3호
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• pp.204-211
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• 2015

새만금 갯벌로부터 xylan과 locust bean gum (LBG)을 분해하는 미생물을 분리하여 그 생화학적 특성과 16S rRNA 서열을 조사한 결과 Bacillus subtilis로 확인되었다. 분리균 YB-30은 konjac이나 LBG가 존재한 상태에서 배양할 경우 mannanase의 생산성이 급격하게 증가되며, 밀기울이 존재하에서는 xylanase의 생산성이 증가되였다. Konjac (3.5%)과 밀기울(1%)이 동시에 첨가된 배지에서 균의 성장이 정지기에 진입하였을 때 mannanase와 xylanase의 생산성이 최고 수준에 도달하였다. 배양상등액의 mannanase와 xylanase는 60℃와 pH 6.0, 55℃와 pH 5.5에서 각각 최대 활성을 보였다. 고온에서는 두 효소 모두 안정하지는 않았으며 xylanase가 mannanase보다 안정성이 낮았다. 밀기울은 쌀기울보다 많은 양의 arabinoxylan을 함유하고 있으므로 배양상등액으로 분해하였을 때 밀기울로부터 생성되는 환원당의 양이 더 많은 것으로 확인되었으며, 이로 보아 B. subtilis YB-30에 의해 생산되는 xylanase와 mannanase는 사료첨가용 효소로 활용 가능성이 높다고 여겨진다.