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Diversity of Lactic Acid Bacteria (LAB) in Makgeolli and Their Production of γ-Aminobutyric Acid (막걸리에서 분리한 Lactic Acid Bacteria (LAB)의 다양성 분석과 γ-aminobutyric acid 생산능 연구)

  • Lee, Hye-Lim;Kang, Ki-Won;Seo, Dong-Ho;Jung, Jong-Hyun;Jung, Dong-Hyun;Kim, Gye-Won;Park, Sun-Young;Shin, Woo-Chang;Shim, Hyoung-Seok;Park, Cheon-Seok
    • Korean Journal of Food Science and Technology
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    • v.47 no.2
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    • pp.204-210
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    • 2015
  • Makgeolli is made from rice or flour, yeast, and nuruk, a fermentation starter. The flavor of makgeolli is affected by sugars, amino acids, organic acids and volatile flavor compounds produced by various microorganisms. In this study, lactic acid bacteria (LAB) were isolated from unsterilized makgeolli samples collected from several provinces in Korea, and then later identified. Under anaerobic conditions, LAB density ranged from $5.0{\times}10^6$ to $1.5{\times}10^8CFU/mL$; yeast density ranged from $2.5{\times}10^7$ to $1.5{\times}10^8CFU/mL$. Of the LAB isolated from makgeolli, 1,126 were analyzed using restriction fragment length polymorphism (PCR-RFLP) analysis of 16S rRNA, which allowed for classification into five groups. Of the 1,126 LABs tested, 130 produced ${\gamma}$-aminobutyric acid (GABA).

Microbial Contamination Analysis for Drinking Water, Foodstuff, and Cooked Food for Foodservice Operation (단체급식소의 음용수, 식재료와 조리식품의 세균 오염분석)

  • Cho, Sun-Kyung;Park, Jong-Hyun
    • Korean Journal of Food Science and Technology
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    • v.44 no.4
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    • pp.478-483
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    • 2012
  • In order to evaluate the microbial safety of the foodstuffs and manus in regards to foodservice operations, the total of 1,051 samples of drinking waters, vegetables, and cooked foods were collected. Total viable count (TVC), coliform group, and pathogens were analyzed. Twenty five percent samples of 441 drinking waters showed high contamination of above 2 log CFU/g TVC. Twenty percent of the waters were contaminated by above one log CFU/g coliform. Forty percent of non-heated food, Moochim, were contaminated by above 5 log CFU/g TVC and 26% of Moochim were contaminated by above 2 log CFU/g coliform. Bacillus cereus and Eschericia coli were detected on boiled rice and kimchi, and B. cereus was detected with a high ratio. Therefore, the foodstuffs and manus regarding foodservice operations appear to be generally safe. However, efficient microbial control for drinking water and Moochim-style food is necessary and in particular, careful management to reduce microbial contamination should be applied to drinking water.

Evaluation of in-vitro Anti-thrombosis and Anti-oxidation Activity of Lees of Takju (Wookukseng) (우국생 탁주 주박의 항혈전 및 항산화 활성)

  • Kim, Mi-Sun;Lee, Ye-Seul;Kim, Jong Sik;Shin, Woo-Chang;Sohn, Ho-Yong
    • Journal of Life Science
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    • v.25 no.4
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    • pp.425-432
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    • 2015
  • This study was performed to develop high-value-added biomaterials for health and beauty products. Extracts of ethanol and hot water and their subsequent organic solvent fractions were prepared from Lees of Wookukseng (LW), a commercialized Korean traditional rice wine. We investigated their activities on blood coagulation, platelet aggregation, hemolysis against human red blood cells (hRBCs), and anti-oxidation. The water content, pH and brix of the LW were 80.3%, 3.94 and 13.0°, respectively. The yield of ethanol extraction (6.62%) was 3.15 times higher than that of hot-water extraction (2.1%), and the ethyacetate fraction (EAF) of ethanol extract showed the highest content of total polyphenol (128 mg/g) among the various fractions. In anticoagulation activity assay, the EAF of ethanol extract showed a 15-fold extension in TT, PT, and aPTT, indicating that the EAFs contain various inhibitory substances against thrombin, prothrombin and coagulation factors. In anti-platelet aggregation activity assay, the butanol fraction and water residue of ethanol extract showed significant inhibition activity. The activities were comparable to aspirin, a commercial anti-thrombosis agent. The above extracts and fractions did not show hemolysis activity against hRBC up to 5 mg/ml, and had radical scavenging activity against DPPH anion, ABTS cation and nitrite. Our results suggest that the active fractions prepared from LW, which has no specific usage until now, have a high potential as novel resources for anti-thrombosis agents.

Gibberellin A4 Producted by Fusarium solani Isolated from the Roots of Suaeda japonica Makino (칠면초의 뿌리로부터 분리된 Fusarium solani에 의해 생산된 지베렐린 A4)

  • Seo, Yeonggyo;You, Young-Hyun;Yoon, Hyeokjun;Kang, Sang-Mo;Kim, Hyun;Kim, Miae;Kim, Changmu;Lee, In-Jung;Kim, Jong-Guk
    • Journal of Life Science
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    • v.22 no.12
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    • pp.1718-1723
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    • 2012
  • Ten endophytic fungi with different colony morphologies were isolated from the roots of Suaeda japonica Makino growing naturally in Suncheon Bay. Plant growth promotion was verified by treating waito-c rice seedlings with culture filtrates from the endophytic fungi. The bioassays showed that the Sj/7/4 fungal strain induced effective growth promotion in the seedlings. The gibberellins (GA) produced by fungal strain Sj/7/4 were analyzed by gas chromatography /mass spectroscopy with selected ion monitoring (GC/MS SIM). The culture filtrate of Sj/7/4 fungal strain was confirmed to contain $GA_4$ through quantitative analysis. The Sj/7/4 fungal strain was identified to determine the internal transcribed spacer (ITS) regions with universal primers ITS-1 and ITS-4 by using polymerase chain reactions (PCR). Molecular analysis of the Sj/7/4 fungal strain showed high similarity to Fusarium solani. The Sj/7/4 fungal strain isolated from the root of S. japonica was therefore designated as F. solani Sj/7/4.

A Study on the Damyang Area Restaurants in Bamboo food village (향토음식 특화 거리의 관광상품화와 활성화 방안 연구 - 담양 죽순 푸드빌리지를 중심으로 -)

  • Kim, Su In;Park, Yeon Jin;Kim, So Young;Chang, Hea Jin
    • Journal of the Korean Society of Food Culture
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    • v.28 no.4
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    • pp.348-355
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    • 2013
  • This study intends to provide preliminary data for improving dining experience in the restaurants of Bamboo food village and help draw up guidelines for the improvement of these dining venues by surveying customer perception and satisfaction in 15-restaurants of the food village. The restaurants were surveyed mainly for satisfaction of the menu, especially, on the signature dishes of Damyang, "ddeokgalbi" (grilled short rib balls) and "daetongbab"-the grilled short rib balls and bamboo rice. The two dishes were more liked by people in the the 20- to 29-year age group with a score of 3.92 and 4.11, respectively. Although the 30-49 age group showed the highest satisfaction score on the fixed price menu, there was no statistically significant difference. The age group of 20-29 also showed the highest satisfaction on plating and table setting with a score of 4.09 and 4.04, respectively, and there was significant difference among age groups in this regard (p<0.05). All the age groups surveyed answered "time-honored taste" should be captured when working on menus, which suggests it should be the first choice for the restaurants in the food village when they develop their menus. When it comes to the restaurant environment, satisfaction on sanitary conditions was significantly different among the groups with a score of 4.21 given by 30-49 age group and 3.88 by the 50 and over group (p<0.05). In the category of service satisfaction, the two aforementioned age groups again showed significant difference in catering to customer needs with a score of 3.99 and 3.63, respectively (p<0.05), whereas welcoming customers and serving food was scored without statistical difference by age. Being asked what needs to be done to strengthen competitive advantage of the restaurants, all the age groups answered "taste" would matter the most while the 20 to 29 and 30 to 49 age groups picked "hygiene" and the 50 and over selected "table setting and ambience" next, which was statistically different with a p value of <0.05. Regarding the competitive advantage of the Korean restaurants in Damyang Bamboo food village, the first two younger groups (20 to 29 and 30 to 49) chose "table setting and ambience" and the eldest (50 and over) age group answered "location wise advantage," indicating significant difference by age. More than 80 percent of the people surveyed were willing to revisit the venues, which suggests improving restaurant environment in Bamboo food village and offering customers a better experience are very likely to build a image of culinary tourism for Damyang.

Different Photosynthetic Responses of Black Cherry (Prunus serotina) with Different Sensitivities to Ambient Ozone Concentrations under Natural Conditions (자연상태에서 대기 중 오존 농도에 상이한 민감성을 가진 Black Cherry(Prunus serotina)의 상이한 광합성 반응)

  • Yun, Myoung-Hui;Chevone, Boris I.
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.10 no.4
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    • pp.132-140
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    • 2008
  • Two different sensitivity classes of black cherry (Prunus serotina) under the natural growing environmental conditions were assessed adjacent to Air Monitoring Station located at Horton research center in Giles County, Virginia, USA. Ambient ozone concentrations, leaf gas exchange, and visible foliar injury were measured on-site during the growing seasons of 2000, 2001, and 2002. Ambient ozone exposures were sufficient to induce typical foliar visible injury corresponding with the reduction in photosynthetic activities only in sensitive black cherry. There were positive correlations between increasing cumulative ozone concentration and percent reduction in maximum net photosynthetic rates ($Pn_{MAX}$) under saturating light conditions and in quantum yield for carbon reduction (${\Phi}CO_2$) of sensitive black cherry compared to tolerant black cherry. There was a negative correlation between chlorophyll content and percent leaf injury in sensitive black cherry. Furthermore, $Pn_{MAX}$ was inversely related to percent leaf injury.

Comparative Study on Maternal Health Status With Island and Land Women in A Middle City Area (중도시내(中都市內) 도서(島嶼)와 육지거주부인(陸地居住婦人)의 모성보건실태비교(母性保健實態比較))

  • Kim, Kyu-Chul
    • Journal of Preventive Medicine and Public Health
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    • v.9 no.1
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    • pp.65-75
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    • 1976
  • To compare the maternal health status between women in island and land, the study was conducted to adjacent land (kukdong) and island (kyonghodong) areas in Yosoo city during April 1976. The results obtained from 174 interviewee in island and 192 in land areas were the same as follow, 1. General characteristics of both area. Age: Land, group 20-29 year of age was the largest. Island, 30-39 year of age was the largest. Education; Low educated group, less than primary grade (land 88.6%, island 93.1%), was predominant in both area. Occupation of herself; agriculture and labor was the main occupation (land 50%, island 82.2%) in both area. Occupation of husband: labor and fishery was general (81.2%) in land, and agriculture and fishery was general (81.6%) in island. Duration of residence; Land, 25.0% being resided here more than 10 years. Island, 64.3% being resided here more than 10 years. 2. Marriage, Pregnancy, and Present children. Average age at marriage; Land, 20.7 years. Island, 20.9 years. Average frequency of pregnancy; Land, 4.4 times. Island, 4.3 times. Wastage of pregnancies: Land, 236 per 1000 pregnancies. Island, 151 per 1000 pregnancies. Wastage occupied by induced abortion; land, 73.5%. island, 60.5%. Number and sex of present children; Land, 3.3 per family, sex ratio 52.4 to 47.6. Island, 3.6 per family, sex ratio 53.3 to 46.7. 3. Prenatal and postnatal care. Prenatal consutation: Land, 16.1% received by doctor or midwife. Island 9.2% received by doctor or midwife. Complications during last pregnancy; Land, 46.6% complained. Island, 51.1% complained. Return to work within 1 week after delivery; Land, 40.6%. Island, 50.6%. 4. Delivery environment Home delivery; Land, 97.4% Island, 97.3%. Delivery attdended by non medical professions at home; Land, 80.2% Island, 93.7%. Solo home delivery; Land, 13.0%, Island, 12.9%. Delivery attended by layman without taking any disinfective preparations; Land, 48.1%, Island, 49.1%. Material mainly used to cut umbilical cord at home; Land, scissors (97.4%). Island, scissora (98.8%). Cord cutting material used without giving any disinfective preparations; Land, 79.9%, Island, 84.0%. Delivery sheets used at home; Cement bag paper (land 50.0%, island, 31.3%). Vinyl sheets (land,17.5%, island, 27.6%). News paper (land, 3.2%. island, 11.7%). No sheets (land 19.5%. island, 12.9%). etc. Maternal Meal; Seaweed soup with rice was the most general in both area. (land 95.3%, island, 91.4%).

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SEED DORMANCY AND GERMINATION BEHAVIOUR OF ECHINOCHLOA COLONA (Echinochloa colona 종자(種子)의 휴면(休眠) 및 발아특성(發芽特性))

  • Chun, J.C.;Moody, K.
    • Korean Journal of Weed Science
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    • v.7 no.1
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    • pp.12-18
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    • 1987
  • Seed dormancy and germination responses to light and gases were determined for Echinochloa colona (L.) Link. E. colons seeds did not require a period of after-ripening for breaking dormancy. Water movement occurred readily across the seed coat. Repeated cycles of hydration and dehydration reduced viability and thence germination. Water imbibition for 24 h increased seed moisture by 21%; seeds returned to their original weight after drying at room temperature for 13 h. Removal of seed-coats increased germination in the dark. Light stimulated germination. Germination at a daylight intensity of 51.9 $Wm^{-2}$ or less was significantly reduced. Germination of seeds which were exposed to light for 1 h each day was significantly less than that of seeds exposed for longer than 2 h a day. Seeds subjected to blue light had delayed and decreased germination compared to seeds exposed to red light. Ethylene or carbon dioxide exogenenously added in the presence of light stimulated germination. The addition of the two gases together had a synergistic effect. In the dark, however, the two gases did not increase germination.

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Selective Action of Root-Treated Oxyfluorfen and Chlomethoxynil (근부처리(根部處理) Oxyfluorfen과 Chlomethoxynil의 선택작용성(選擇作用性))

  • Lee, Jeung-Joo
    • Korean Journal of Weed Science
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    • v.14 no.2
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    • pp.107-111
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    • 1994
  • Selective action of root-treated oxyfluorfen [(2-chloro-4-thrifluoromethylphenyl)-3'-ethoxy-4'-nitrophenyl ether] and chlomethoxynil [2, 4-dichlorophenyl-3'-methoxy-4'-nitrophenyl ether] were investigated. Oxyfluorfen showed greater activity to all plant species than chlomethoxynil. $^{14}C$-oxyfluorfen was little metabolized in roots of the plant species and more slowly absorbed than $^{14}C$-chlomethoxynil. These results suggest that herbicidal activity of oxyfluorfen at the site of action is higher than chlomethoxynil. In the tested plants, rice, barnyardgrass, sorghum, and corn were absorbed less of the oxyfluorfen and chlomethoxynil than the broad leaf plant species. However, no clear relationship was observed between a degree of tolerance and absorption and metabolism of both herbicides by the plant species.

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Studies on the Mycelial Cultivation and the Rhizomorph Production of Armillaria mellea (Armillaria mellea의 균사배양 및 균사속 생산에 관한 연구)

  • Hong, Jai-Sik;Kim, Myung-Kon;So, Gyu-Ho;Kim, Young-Hoi
    • The Korean Journal of Mycology
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    • v.18 no.3
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    • pp.149-157
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    • 1990
  • Five strains of Armillaria mellea were collected from the forest of Chonbuk province and isolated from the tissue of fruit bodies. Nutritional and environmental characteristics of mycelial cultivation and rhizomorph production of Armillaria mellea isolated were determined in sawdust media, woody inocula and soils. The sawdust media of Styphnolobium japonicum, Culhamia simplex, Populus monilifera and Populua davidiana were proper for mycelial growth. The ranges of optimum pH, temperature and moisture content for mycelial growth were in the range of $4.5{\sim}5.0$, ${\sim}25^{\circ}C$ and $65{\sim}70%$, respectively. Among the various additives and inorganic salts added, 10% rice bran and 3% $CaCO_3$ were effective to mycelial growth. The woody inocula of Styphnolobium japonicum, Culhamia simplex, Quercus acutissima and Quercus veriabilis were proper for rhizomorph production. The ranges of optimum pH, moisture content and temperature for rhizomorph production were in the range of $4.5{\sim}4.9,$ $45{\sim}55%\;(w/w)$ and $20{\sim}24^{\circ}C$, respectively. Distribution of rhizomorphs in soil was varied with depth, but the main concentration occurred in the range of $7.5{\sim}12.5\;cm$. They were rarely found below 25.0 cm.

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