• Asian-Australasian Journal of Animal Sciences
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• 제23권3호
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• pp.292-301
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• 2010

Follicle-stimulating hormone (FSH) is a pituitary glycoprotein hormone that is encoded by separate alpha- and betasubunit genes. It plays a key role in stimulating and regulating ovarian follicular development and egg production in chicken. FSH signal transduction is mediated by the FSH receptor (FSHR) that exclusively interacts with the beta-subunit of FSH, but characterization of prokaryotic expression of the FSHb gene and its effect on the expression of the FSHR gene in local chickens have received very little attention. In the current study, the cDNA fragment of the FSHb gene from Dagu chicken was amplified using reverse transcription polymerase chain reaction (RT-PCR), and inserted into the pET-28a (+) vector to construct the pET-28a-FSHb plasmid. After expression of the plasmid in E. coli BL21 (DE3) under inducing conditions, the recombination protein, $FSH{\beta}$ subunit, was purified and injected into the experimental hens and the effect on the mRNA expression levels of the FSHR gene was investigated. Sequence comparison showed that the coding region of the FSHb gene in the local chicken shared 99%-100% homology to published nucleotides in chickens; only one synonymous nucleotide substitution was detected in the region. The encoded amino acids were completely identical with the reported sequence, which confirmed that the sequences of the chicken FSHb gene and the peptides of the $FSH{\beta}$ subunit are highly conserved. This may be due to the critical role of the normal function of the FSHb gene in hormonal specificity and regulation of reproduction. The results of gene expression revealed that a recombinant protein with a molecular weight of about 19 kDa was efficiently expressed and it was identified by Western blotting analysis. After administration of the purified $FSH{\beta}$ protein, significantly higher expression levels were demonstrated in uterus, ovary and oviduct samples (p<0.05). These observations suggested that the expressed $FSH{\beta}$ protein possesses biological activity, and has a potential role in regulation of reproductive physiology in chickens.

• 대한수의학회지
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• 제40권3호
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• pp.551-561
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• 2000

Swine respiratory diseases have induced severe economic losses in swine industry worldwide. Several methods have been developed and applied to prevent and control the disease. However, those are still problematic in swine industry. Recently, the use of egg yolk antibodies with several advantages was introduced and applied to control diseases in animal as well as human. As the first step of the use of egg yolk antibodies in the control of the swine respiratory diseases, we investigated the immunogens of the causative agensts of the diseases and immune response in egg yolk of hens immunized with them. Bacterial antigens prepared from Bordetella bronchiseptica, Pasteurella multocida 3A and 4D, and Actinobacillus pleuropneumaniae serotype 2 and 5 were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Western blot and toxicity test in mice. The antigens were injected into laying hens in order to produce antibodies against them in egg yolk. After chickens were immunized three times in 2 weeks interval, the profile of antibody production was examined by ELISA. The production of antibody in egg yolk was started in 2 weeks after the first injection, reached peak in 6-8 weeks and maintained until 12 weeks. Of two adjuvants used in this study, ISA70 was more effective than aluminum hydroxide gel in enhancing immunogenecity, laying rates and safety in hens. These results suggested that egg yolk antibodies could be a good source for production of antibodies specific to pathogenic bacteria inducing respiratory diseases of swine.

• Journal of Nutrition and Health
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• 제42권3호
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• pp.291-299
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• 2009

This study was carried out to examine the proportion of fatty acids and contents of trans fatty acid in commercial processed foods in Jeon-Buk area. Contents of trans fatty acid in bakery products ranged from 0.2% to 1.0% per 100 g food. Especially garlic bread and pastry contained the highest amount. Popcorns contained trans fatty acid from 0.0% to 13.4%. Among them, instant popcorns for microwave contained the highest amount, while trans fatty acid was hardly detected in popcorns for theater. And trans fatty acid detected in popcorns was mostly in the form of 18 : 1 trans. The content of trans fatty acid in pizza was nearly 0.2%. Amounts of saturated fatty acid and unsaturated fatty acid were similar between each type of pizza and frozen pizza. Fritters such as fried chickens and vegetables contained trans fatty acid less than 0.2% per 100 g food.

• 한국가금학회지
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• 제50권4호
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• pp.231-240
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• 2023

전염성 F낭병은 닭에서 F낭의 위축 및 염증이 나타나고, 이로 인해 면역억제를 특징으로 하며, 급성, 높은 점염성을 가지는 질병이다. VP2 유전자의 염기서열 분석을 통해 유전형이 분류되고, 유전형에 따라 항원성 및 병원성에 큰 차이를 나타내고 있다. 최근 중국에서 발생한 항원 변이주의 경우, 기존의 미국형 항원 변이주와는 다른 분자 특성을 보이는 것으로 나타났다. 최근 국내에서도 이러한 중국형 항원 변이주가 발생하고 있고, 따라서 본 연구에서는 국내 양계농장에서 분리된 항원 변이주의 유전체 분석을 통한 분자적 특성을 확인하였다. 국내 육계 및 토종닭으로부터 4개의 항원 변이주를 RT-PCR을 통해 확인하였고, chorioallantoic membrane 접종을 통해 분리하였다. VP2 유전자의 hypervariable region의 분석 결과, 4개의 항원 변이주 중 1개는 미국형 항원 변이주로 확인되었고, 이는 2009년 국내에서 보고된 09D243 균주와 유사한 것으로 나타났으며, 3개의 중국형 항원 변이주는 최근 국내 및 중국의 유행주와 유사한 것으로 확인되었다. 우리의 결과에서 국내 양계농장에서 중국형 및 미국형 항원 변이주가 퍼져 있음을 확인하였고, 또한 백신을 접종한 농장에서의 발생도 이뤄지고 있어 항원 변이주에 대한 상용화 백신의 보호 효능에 대한 연구도 필요합니다.