• Title/Summary/Keyword: Rhodosporidium

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Characteristics of Unrecorded Yeasts, Rhodosporidium fluviale, Rhodosporidium paludigenum, Candida sp. 80-J-3 and Kluyveromyces thermotolerans Isolated from Wild Flowers in Korea (국내 야생화들로부터 국내 미기록 효모, Rhodosporidium fluviale, Rhodosporidium paludigenum과 Candida sp. 80-J-3, Kluyveromyces thermotolerans의 특성)

  • Hyun, Se-Hee;Lee, Hyang Burm;Lee, Jong-Soo
    • The Korean Journal of Mycology
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    • v.41 no.3
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    • pp.181-184
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    • 2013
  • Rhodosporidium fluviale 201-C-1 and Kluyveromyces thermotolerans 150-JE-2(1) were isolated from wild flowers in Geumsan-gun, Chungcheongnam-do and Wando-gun, Jeollanam-do, respectively. Rhodosporidium paludigenum 86-J-1 and Candida sp. 80-J-3 were also isolated from flowers in Jeju island, Korea. Characteristics for these yeasts were not reported before. Therefore, morphological and physiological characteristics for these yeasts were investigated in this study. R. fluviale 201-C-1 was halophilic asporogenous yeast and formed pseudomycelium. It also grew at $30^{\circ}C$ and pH 4.0-6.0. K. thermotolerans 150-JE-2(1) was sugar-tolerant and halotolerant asporogenous yeast. It formed pseudomycelium and showed positive urease activity. Candida sp. 80-J-3 and R. paludigenum 86-J-1 were asporogenous yeasts, and grew well in yeast extract-peptone dextrose(YPD) medium, potato-dextrose medium as well as vitamin-free medium and YPD medium containing 5% NaCl.

Purification of Internal Invertase in Rhodosporidium toruloides Mating Type A Cells (Rhodosporidium toruloides의 접합형 A 세포내 Invertase의 정제)

  • Jeong, Yong-Kee;Ryu, Beung-Ho
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.21 no.6
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    • pp.725-730
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    • 1992
  • The internal invertase was purified from cell free extract of Rhodosporidium toruloides IFO 0559-M-919 by acid precipitation, ion-exchange chromatography and gel filtration to the unique enzyme protein on disc electrophoresis. We have found out that molecular weight of purified internal invertase was 90,000 by gel filtration and the purified enzyme was protein with 4 homogeneous subunits appearing as single band of 22,000daltons on SDS-polyacrylamide gel electrophoresis.

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Mating-type-specific inhibition of phosphorylation by sexual pheromone (Rh. A) on heterobasidiomycetous yeast Rhodosporidium toruloides. (이담자효모 Rhodosporidium toruloides의 성pheromone(Rh.A)에 의한 성접합형 특이적 인산화 저해 반응)

  • 정영기
    • Journal of Life Science
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    • v.7 no.4
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    • pp.322-328
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    • 1997
  • Two phosphorylated proteins having molecular weights of 57kD and 72kD were detected from the slubilized membrane protein fraction of mating type a cells of Rhodosporidium toruloides which belongs to heterobasidiomycetous yeast. The phosphorylation of the protein was inhibited by a sexual pheromone, Rhodotorucine A (Rh. A), which is secreted from mating type a cells. On the other hand, counterpart mating type A cells and M-39 strain which is a styerile mutant derived from a cells, had also the same two phosphorylated proteins, However, the phosphorylation of the protein from A cells, and M-39 strain were not inhibited by the Rh. A. It suggests that inhibition of the phosphorylation reaction by the Rh. A in mating type a cells is a mating-type-specific reaction that relate to transduction mechanism of sexual pheromone signaling.

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Ubiquinone compounds of the strains in Leucosporidium scottii and its related taxa (담자균 효모(酵母) Leucosporidium scottii와 관련 분류군균주(分類群菌株)의 ubiquinone 물질)

  • Joo, Woo-Hong
    • The Korean Journal of Mycology
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    • v.19 no.4
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    • pp.258-265
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    • 1991
  • Ubiquinone compounds of strains in Leucosporidium scottii, Leucosporidium fellii, Leucosporidium lari-marini, and Rhodosporidium fluviale were analyzed by the high performance liquid chromatography. The Q-9 or Q-l0 compounds, independent on mating or self-sporulating type, were determined in the analyses of Leucosporidium scottii strains. Particularly, there is heterogenous in the quinone compounds in the same strain of L. scottii. These results showed reassessment of the quinone compounds as a taxonomic criterion; L. fellii had the Q-9 compound, L. lari-marini the Q-8 compound, and R. fluviale the Q-10 compound, Taxonomic position of L. lari-marini should be elucidated on the basis of analysis of Q-compound with other aspects.

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Production of Chiral (S)-styrene Oxide by Rhodosporidium sp. SJ-4 which has an Epoxide Hydrolase Activity (에폭사이드 가수분해효소를 갖는 Rhodosporidium sp. SJ-4를 이용한 광학활성 (S)-styrene Oxide의 생산)

  • Yoo, Seung-Shick;Lee, Eun-Yeol;Kim, Hee-Sook;Kim, Jung-Sun;Oh, You-Kwan;Park, Sung-Hoon
    • Journal of Institute of Control, Robotics and Systems
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    • v.11 no.10
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    • pp.857-863
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    • 2005
  • A yeast strain utilizing styrene epoxide as a sole carbon and energy source was isolated from soil samples for the production of enantiopure of styrene epoxide by kinetic resolution. The strain, identified as a Rhodosporidium toruloides SJ-4, expressed an epoxide hydrolase which preferentially converted (R)-styrene epoxide into the corresponding diol. A maximum activity of 135 U/L was observed when biomass (dry cell mass) reached 6.7 g/L at 21 h of batch culture. Under the partially optimized reaction conditions ($35^{\circ}C$ and pH 8.0), the optically pure (S)-styrene epoxide was obtained with the yield of 21% when the initial substrate concentration was 100 mM. The reaction was completed at 9 h.

Production of Chiral Phenyl Oxirane by Rhodosporidium toruloides in Hollow-fiber Reactor (Rhodosporidium toruloides를 이용한 Hollow-fiber 반응기에서의 광학활성 Phenyl Oxirane 생산)

  • 김희숙;박성훈;이은열
    • Journal of Life Science
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    • v.13 no.6
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    • pp.788-793
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    • 2003
  • Production of chiral phenyl oxirane by Rhodosporidium toruloides SJ-4 was investigated. Racemic phenyl oxirane was kinetically resolved by enantioselective hydrolysis reaction by epoxide hydrolase of R. toruloides in two-phase hollow-fiber reactor system. Dodecane with high concentration of the racemic substrate passed through the lumen side and cell suspension was recirculated through the shell side of the hollow fiber reactor For the removal of phenyl-1, 2-ethandiol to reduce the product inhibition to biocatalysts, another hollow-fiber reactor was employed to extract the diol. Racemic phenyl oxirane up to 300 mM was enantioselectively resolved with high enantiopurity (>99% ee) in hollow-fiber reactor system.

Oleaginous Yeast Rhodosporidium toruloides as a Tool for Rapid Evaluation of Anti-Obesity Candidates: Inhibitory Effect of Persimmon Leaf Fermentate on Lipid Accumulation

  • Lee, Nam Keun
    • Journal of Microbiology and Biotechnology
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    • v.27 no.10
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    • pp.1773-1777
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    • 2017
  • The aim of this study was to examine the efficiency of Rhodosporidium toruloides as a new tool to evaluate the triglyceride (TG) reduction effects of anti-obesity candidate materials. Unfermented and fermented persimmon leaf hot water extracts (UFPLE and FPLE) were used as anti-obesity agents. The content of TG in R. toruloides treated with FPLE was less than those with UFPLE by about 11% (p < 0.05) relative to the control (R. toruloides incubated in YPD medium without the agents). Fat reduction in 3T3-L1 cells achieved by FPLE was about 13% higher than that achieved by UFPLE.

이담자균 효모 Rhodosporidium toruloides에서 Rhodotorucine A에 의한 막단백질 인산화의 저해와 Trigger Peptidase의 관련성

  • 정영기;이태호;류병호
    • Microbiology and Biotechnology Letters
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    • v.24 no.6
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    • pp.641-646
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    • 1996
  • [$\gamma$-$^{32}$P]ATP was used to test phosphorylation of membrane proteins of mating type a cells of heterobasidiomycetous yeast Rhodosporidium toruloides separated by non-denaturing electrophoresis. The phosphoprotein was observed in the membrane proteins. The phosphorylation was inhibited by the pheromone rhodotorucine A (Rh. A) secreted by mating type A of the yeast. Rh. A didn't inhibit the phosphorylation in the presence of a trigger peptidase (TPase) inhibitor, antipain. Partially digested Rh. A by trypsin maintained the phosphorylation inhibitory activity. These results show that TPase activity plays an important role in the transduction of pheromone signal in the yeast.

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Enantioselective Hydrolysis of Racemic Styrene Oxide by Epoxide Hydrolase of Rhodosporidium kratochvilovae SYU-08

  • Lee, Ji-Won;Lee, Eun-Jung;Yoo, Seung-Sik;Park, Sung-Hoon;Kim, Hee-Sook;Lee, Eun-Yeol
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.8 no.5
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    • pp.306-308
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    • 2003
  • Enantioselective hydrolysis for the production of chiral styrene oxide was investigated using the epoxide hydrolase activity of a newly isolated Rhodosporidium kratochvilovae SYU-08. The effects of reaction parameters - buffer type, pH, temperature, initial substrate concentrations, phenyl-1,2-ethanediol concentrations on hydrolysis rate, and enantioselectivity - were analyzed. Optically active (S)-styrene oxide with an enantiomeric excess higher than 99 % was obtained from its racemate. with a yield of 38 % (theoretically 50% maximum yield) from an initial concentration of 80 mM.

Effect of Sexual Pheromone on Phosphoryation of Membrane Protein in Heterobasidiomycetous Yeast Rhodosporidium toruloides (이담자효모 Rhodosporidium toruloides의 막단백질 인산화와 성 Pheromone, Rhodotorucine A의 작용)

  • 정영기
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.26 no.6
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    • pp.1246-1251
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    • 1997
  • When the membrane protein fraction of mating type a cells of heterobasidiomycetous yeast R. toruloides was phosphorylated in vitro, two phosphorylated proteins of 72Kd and 57Kd were detected on SDS-polyacryamide gel. The phosphorylation reaction was inhibited by rhodotorucine A(Rh. A) which is a sexual pheromone secreted by mating type A cells. The inhibition of phosphorylation by Rh. A was dependent on $Ca^{2+}$, and independent on $Mg^{2+}$ or calmodulin. When adding trigger peptidase(TPase) inhibitor, antipain, no inhibition of phosphory was observed. Also, by adding the trysin-digested product of Rh. A, the phosphorylation was inhibited as the action of Rh. A. From these results, it is expected that the inhibition of membrane protein phosphorylation should be caused by the digested product of Rh. A with TPase.

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