• Title/Summary/Keyword: Rhodospirillum rubrum N-1

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Deodorization of Swine Wastewater by Rhodospirillum rubrum N-1 (Rhodospirillum rubrum N-1을 이용한 양돈폐수의 악취제거)

  • Choi, Kyung-Min;Kim, Jong-Seung
    • Journal of the Korea Organic Resources Recycling Association
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    • v.6 no.1
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    • pp.13-20
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    • 1998
  • Rhodospirillum rubrum N-1 was inoculated to manipulated swine wastewater of 20,000 mg/L as Biochemical Oxygen Demand (BOD) to study the effect of aeration on swine wastewater deodorization. Biological and physico-chemical parameters were determined at 1 day interval for 9 days. Removals of BOD, volatile fatty acids (VFAs), and phosphate were 54.6%, 87.0%, and 54.5%, respectively. No significant changes were observed in the concentrations of total nitrogen, total phosphorus, nitrate, nitrite, hydrogen sulfide, and mercaptane.

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양돈 폐수 처리를 위한 Rhodospirillum rubrum N-1의 분리 및 특성 연구

  • Choi, Kyung-Min;Yang, Jae-Kyung;Park, Eung-Roh;Bang, Keuk-Soo;Lee, Sung-Taik
    • Microbiology and Biotechnology Letters
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    • v.25 no.3
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    • pp.322-327
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    • 1997
  • Photosynthetic bacterium strain N-1 was isolated from the eutrophic wet soil and identified as Rhodospirillum rubrum. The optimum conditions for the cultivation of R. rubrum N-1 were estimated as 0.2% (w/v) of sodium acetate, 0.2% (w/v) of sodium propionate, 0.2% (w/v) of sodium butyrate in the Lasscelles basal medium at 30$circ$C, pH 7.0 under 4000 lux of the illumination. The removal efficiencies of total organic acid and chemical oxygen demand (COD$_{Cr}$) in swine wastewater were 80% and 87%, respectively, when 10% (v/v) of R. rubrum N-1 was inoculated.

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Study of metabolite production conditions by using the resting cells of Rhodospirillum rubrum N-1 (Rhodospirillum rubrum N-1의 휴지균체를 이용한 균체 대사산물의 생산 조건 연구)

  • 최경민;양재경
    • Journal of environmental and Sanitary engineering
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    • v.14 no.3
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    • pp.107-115
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    • 1999
  • The effectiveness of resting cells of a photosynthetic bacterium, Rhodospirillum rubrum N-1, was investigated on the production of extracellular ${\delta}-aminolevulinic$ acid(ALA). The ALA generating system required 3hr-incubation in the presence of 10mg of resting cells per ml to obtain the maximal yield of extracellular ALA. and also, under this condition the effect of ALA inducers, i.e., 30mM levulinic acid (LA) and L-glutamic acid($C_5$ pathway precursor) was relatively higher than that of produced extracellular ALA($83{\mu}M$). The volume of system and proper cell density appeared to be important factors for the effective production of extracellular ALA.

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Production of Photodynamic Herbicide by Photosynthetic Bacteria (광합성균주에 의한 제초활성 물질의 생산)

  • Choi, Kyung-Min;Lee, Sung-Taik
    • Journal of the Korea Organic Resources Recycling Association
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    • v.5 no.1
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    • pp.25-32
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    • 1997
  • The effect of levulinic acid (LA) and biosynthetic precursors of ${\delta}$-aminolevulinic acid (ALA) on the production of extracellular ALA was examined for the cells of soil derived Rhodospirillum rubrum N-1 belonged to the genus Rhodospirillaceae. The extracellular yield of ALA was increased to 23 fold (45 mg/l) from the basal condition (Lascelles' medium without L-glutamate) by successive addition of LA at initial (10 mM) and mid-log stage (30 mM) of cell cultivation. In addition to initial/mid-log mutual supplementations of LA (10 mM/30 mM) and glutamate (30/30 mM), respectively, by means of alternative feeding 10 mM $C_4$-precursors at mid-log phase of culture the extracellular ALA content was reached to 75 mg/l (40 fold).

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A Fundamental Study on Utilization of Photosynthetic Bacteria Metabolites (광합성세균 균체대사산물의 자원화에 대한 기초적 연구)

  • Choi, Kyung-Min;Yang, Jae-Kyung;Park, Eung-Roh;Bae, Jin-Woo;Seo, Yong-Ki;Lee, Sung-Taik
    • Journal of the Korea Organic Resources Recycling Association
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    • v.5 no.1
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    • pp.63-69
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    • 1997
  • The role of L-glutamic acid, a precursor of $C_5$ ALA biosynthetic pathway, on the production of 5-aminolevulinic acid (ALA) has been described in cells of Rhodospirillum rubrum N-1. To the Lascelles basal medium the addition of both 30 mM L-glutamicacid and 20 mM levulinic acid (LA) provided to increase the extracellular ALA yield up to 40 fold (76 mg/l). By the addition of both 60 mM glycine and succinic acid, precursorsof $C_4$ ALA biosynthetic pathway, at middle log phase of cell growth ALA yield was increased 27 fold (52 mg/l) although the celt growth was inhibited to a certain extent.

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