• The Korea Journal of Herbology
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• v.33 no.6
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• pp.35-42
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• 2018

Objectives : The aim of this study was to investigate whether the extract of Coptidis Rhizoma inhibits inflammation in chronic cold stress (CCS)-exposed mice or not. Methods : Coptidis Rhizoma extract (CRE) was made by reflux with distilled water. Male ICR mice (7 weeks old) were divided randomly into 5 groups: (1) control, (2) CCS, (3) CCS+CRE 100 mg/kg, (4) CCS+CRE 300 mg/kg, (5) CCS+CRE 1,000 mg/kg groups. Mice were orally administered once a day for 14 days starting from 1 day before CCS. Group (2)-(5) were exposed to CCS conditions that maintained at $4^{\circ}C$ for 2 h once a day for 14 days. The levels of serum cortisol and hypothalamic prostaglandin E1 (PGE1) and PGE2 were measured by enzyme-linked immunosorbent assay kit. The expression levels of several pro-inflammatory factors like heat shock protein 70 (HSP70), c-fos, and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) were measured by western blot analysis in mouse hypothalamus. Results : Oral administration of CRE 1,000 mg/kg significantly suppressed the increase of serum cortisol levels in mice exposed to CCS. CCS-exposed mice had significantly increased the expression of HSP70, c-fos, and NF-kB in hypothalamus, while CRE treatment significantly attenuated the elevation of these pro-inflammatory factors. The ratio of PGE2/PGE1 was also higher in CCS-exposed mice than control group. CRE treatment significantly reduced the increase of PGE2/PGE1 ratio induced by CCS. Conclusion : These findings suggest that Coptidis Rhizoma may work as a potential agent to modulate inflammatory responses under the condition of cold adaptation formed by CCS.

• Journal of Pharmacopuncture
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• v.11 no.1
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• pp.99-107
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• 2008

Objectives : This experimental study was performed to investigate the effect of herbal eye drops(Sean-tang, Jinpi-san, Tangpo-san, Copitdis Rhizoma) on Staphylococcus aureus keratitis. Methods : After administering various herbal eye drops on Staphylococcus aureus, I measured MIC and the size of inhibition zone. MIC was measured by dropping from 20 to $50{\mu}{\ell}$ according to density. Anti-bacterial potency was measured by the size of inhibition zone with change of volume under 2 days culture condition. Also continuous antibacterial potency of herbal eye drops was measured by the size of inhibition zone according to 2 days and 6 days culture each under the $50{\mu}{\ell}$condition. Results : 1. MIC on Staphylococcus aureus in Sean-tang, Jinpi-san and Tangpo-san was 100%, $20{\mu}{\ell}$. 2. MIC on Staphylococcus aureus in Coptidis rhizoma was 10%, $30{\mu}{\ell}$. 3. MIC on Staphylococcus aureus in Cravit was 0.1%, $50{\mu}{\ell}$. 4. Under the 2 days culture condition, the size of inhibition zone on Staphylococcus aureus by volume for Sean-tang was 12.0mm in $50{\mu}{\ell}$, Jinpi-san was 19.0mm in $50{\mu}{\ell}$, Tangpo-san was 15.0mm in $50{\mu}{\ell}$, Coptidis rhizoma was 32.7 mm in $50{\mu}{\ell}$ and Cravit was 31mm in $50{\mu}{\ell}$, Coptidis rhizoma showed the highest anti-bacterial potency. 5. Under the $50{\mu}{\ell}$ condition, the size of inhibition zone on Staphylococcus aureus by 2 and 6 culture days for Sean-tang was 12.0mm, Jinpi-san was 19.0mm, Tangpo-san was 15.0mm, Coptidis rhizoma was 32.7mm and Cravit was 31.0 mm, which showed sameness anti-bacterial potency in 2 days and 6 days. Conclusions : The present author think that the herbal eye drops can be used to cure Staphylococcus aureus keratitis and if further study is performed, the use of the herbal eye drops will be valuable and beneficial in the clinical medicines.

• The Journal of Korean Medicine
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• v.32 no.6
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• pp.1-9
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• 2011

Objectives: We performed this study to compare the antidiabetic effects of Coptidis Rhizoma (CR) and its major component berberine with gliclazide. Materials and Methods: Diabetic rats induced by injection of streptozotocin (STZ) 55mg/kg were treated with CR 100, 200, 400mg/kg and berberine 100mg/kg. After rats were treated for 5 days, serum glucose, total cholesterol, triglyceride, BUN, creatinine and antioxidant levels were determined. Results: The cytotoxic effects of CR (0.1, 0.01, and 0.001mg/mL), berberine and gliclazide ($0.1{\mu}M$, $1{\mu}M$, and $10{\mu}M$) were tested in rat insulinoma (RIN) cells induced with 5mM STZ. The levels of fasting blood glucose, total cholesterol, triglyceride, BUN and creatinine of CR and berberine treated groups were reduced as much as that of gliclazide group in comparison to control groups, whereas total antioxidant levels increased. In vitro experiments showed that CR and berberine have a cytoprotective effect on RIN cells.

• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.196-200
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• 2002

Compositions of four kinds of products inhibit aging and protect many kinds of disease through eliminating. These compositions inhibit growth of harmful bacteria. such as Bacteroides fragilis. Clostridium perfringens. Staphylococcus aureus, Listeria monocytogenes. Vibrio parahaemolyticus etc. Bacteroides fragilis was controlled by mixing SCUTELLARIA BAICALENSIS GEORGE+CRATAEGI FRUCTUS+PAEONIA JAPONICA+SCHIZANDRAE FRUCTUS. SCUTELLARIA BAICALENSIS GEORGE+CRATAEGI FRUCTUS+PAEONIA JAPONICA+CORNI FRUCTUS. etc. and Clostridium perfringens was SCUTELLARIA BAICALENSIS GEORGE+PHELLODENDRI CORTEX+EPIMEDII HERBA+ASTRAGALI RADIX. SCUTELLARIA BAICALENSIS GEORGE+EPIMEDII HERBA +ASTRAGALI RADIX+GLYCYRRHIZAE RADIX.ste. Listeria monocytogenes was NELUMBO NUCLFERA GAERTNER+SCUTELLARIA BAICALENSIS GEORGE+COPTIDIS RHIZOMA+PAEONIA JAPONICA, NELUMBO NUCLFERA GAERTNER+SCUTELLARIA BAICALENSIS GEORGE+COPTIDIS RHIZOMAtSOPHORAE FLOS. etc. and Vibrio parahaemolyticus was ACANTHODANACIS CORTEX +ASTRAGALI RADIX+PHELLODENDRI CORTEX+COPTIDIS RHIZOMA ACANTHODANACIS CORTEX +ASTRAGALI RADIX + PHELLODENDRI CORTEX+PAEONIA JAPONICA. We expected it as a functional food economical and easy type of taking.

• Journal of Microbiology and Biotechnology
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• v.11 no.2
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• pp.350-353
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• 2001

Porcine pneumonia is caused by Pasturella multiocida and Actinobacillus pleuropneumoniae. To identify a potent drug for antipneumonia therapy, several herbal compounds showing antibacterial effects were screened, and it was found that a methanol extract of Coptidis rhizoma root stem exhibited activity against both pneumonia-causing bacteria. Using an activity-guided fragmentation procedure, an isoquinoline alkaloid was isolated which would be responsible for the antibacterial activities against P. multocida and A. pleuropneumoniae.

• Journal of Pharmaceutical Investigation
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• v.22 no.1
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• pp.55-62
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• 1992

In order to measure the bioavailability of Coptidis Rhizoma preparations, berberine was extracted by the mixture (1:1 v/v) of methanol and water from Coptidis Rhizoma and formulated into pills with some excipients. Disintegration time, dissolution rate, intestinal absorption rate, antibacterial effect, and serum concentration were measured. All pills were disintegrated about 60 minutes in gastric and intestinal juices and disintegration time did not change upon storage at $40^{\circ}C$ for 30 days. The pills manufactured using the extract which was extracted with the solvent containing dilute sulfuric acid showed faster dissolution and absorption from the small intestine than the other samples. It also showed not only the largest area under the serum concentration time curve and relative bioavailability, but also more potent antibacterial effect against gram-positive and gram-negative bacterias than the other samples. The correlation of the dissolution rate with absorption rate constant or $t_{max}$ showed a significant linear relationship.

• The Journal of Pediatrics of Korean Medicine
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• v.28 no.2
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• pp.88-102
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• 2014

Objectives This study is to know the current usage of Ha-Taedok Method in Korean pediatrician. Methods 69 questionnaires were statistically analyzed by using PASW Statistics 18. Results 55 Korean Medicine doctors knew Ha-Taedok Method, but only 2 doctors were using Ha-Taedok Method in their practice. The doctors tend not to use Ha-Taedok Method because it was hard to get permission from the patient's caregiver. 11 Korean Medicine doctors used Ha-Taedok Method to their own children though. The pediatricians felt satisfied from the effect of Ha-Taedok Method especially because it can prevent from disease. They reported no side effect and adverse effect. Coptidis Rhizoma is a preferred herb to use along with Ha-Taedok Method. For a better result from Ha-Taedok Method, it is recommended to start within 3 days old of patient. Conclusions Ha-Taedok Method may be effective as preventive method.

• Proceedings of the PSK Conference
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• 2001.10a
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• pp.300.1-300.1
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• 2001

• Journal of Technologic Dentistry
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• v.23 no.1
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• pp.75-83
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• 2001

This study was conducted to find antibacterial agent against growth of dental caries bacteria. St. mutans KDJ-50 from various medicinal plants of which safety was indetified. Medicinal plants used in this study was dried and grinded after purchased at Daegu yakryung sijang and Kyungsan jungang sijang. Medicinal plants extracted with 80% ethanol at boiling point for 3 hrs was used as antibacterial agent after freeze dried. This study was conducted to find antibacterial agent against growth of dental caries bacteria. St. mutans KDJ-50 from 32 medicinal plants of which safety was indetified. The result of using paper disc method. Caryophylli Flos. Coptidis Rhizoma. Phellodendri Cortex. Schizamdrae Fructs. Myristicae Semen, Crataegi Fructus and Acori Graminei Rhizoma was selected as antibacterial agent. The result of viable cell counting method. the antibacterial activity of Caryophylli Flos was highest among tested 7 medicinal plants followed by Phellodendri Cortex and Coptidis Rhizoma. The extinction effect of the St. mutans by Caryophylli Flos was shown with the addition of 0.5 (w/v) in the medium. The high antibacterial activity was acquired at high extraction temperature and long extraction temperature. The antibacterial activity of Caryophylli Flos was not effected by the concentration of ethanol.

• Herbal Formula Science
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• v.21 no.2
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• pp.81-89
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• 2013

Objectives : The purpose of this study is to observe the effect of Coptidis Rhizoma (CCE-extract of C. chinensis Rhizome) in induction of immune protein on mouse macrophages. Methods : To analyze cytokines interleukin(IL)-$1{\alpha}$, IL-3, IL-9, IL-12p40, IL-13, IL-17, Monocyte Chemoattractant Protein(MCP)-1 induced by macrophages, mouse macrophages were incubated with CCE and was measured. Results : IL-$1{\alpha}$ measurement, CCE showed significant inhibition only at concentration level of 200 ${\mu}g/mL$. IL-3, MCP-1 measurement, CCE showed significant inhibition only at concentration level of 100, 200 ${\mu}g/mL$. IL-9 measurement, CCE showed significant inhibition only at concentration level of 50 ${\mu}g/mL$. IL-13 measurement, CCE showed significant inhibition only at concentration level of 50, 100, 200 ${\mu}g/mL$. The IL-12p40, IL-17 levels indicated no changes at 25, 50, 100, 200 ${\mu}g/mL$ on mouse macrophages. Conclusions : CCE did not significantly increased inflammatory cytokines IL-$1{\alpha}$, IL-3, IL-9, IL-12p40, IL-13, IL-17, Monocyte Chemoattractant Protein(MCP)-1 on mouse macrophages. It was verified CCE does not trigger cytokine related hypersensitivity reaction of organism or exacerbation of acute/chronic inflammatory disease.