• 대한본초학회지
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• 제21권4호
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• pp.171-176
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• 2006

Objectives : A morphological classification among Rheum palmatum Linne, Rheum undulatum Linne and Rumex crispus was made through microscopic observation. Methods : The slice of the tested material made by paraffin section technique was colored with Safranine Malachite Green contrast methods, and then observed and photographed by olymphus-BHT. Results: 1. Stellate spots were found on the intersection of Rheum palmatum, composed of heteromorphy vascular bundles. 2. Stellate sponts were not found in Rheum undulatum and Rumex crispus, because they do not have heteromorphy vascular bundles. Conclusion : Rheum palmatum can be distinguished with Rheum undulatum and Rumex crispus, based on the presence of Stellate spots on its intersection.

• 대한약리학회지
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• 제8권1호
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• pp.77-87
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• 1972

Korean rheum (Rheum undulatum Linne) as one of the botanical crude drugs which belong to polygonaceae family has been extensively applied in Chinese medicine during the last decades. It has been particularly used in cathartic and gastric tonic among the folk remedies. There are, however, a few reports with regard to the pharmacological effects on the motility of intestines of several animals. The authors hereby paid attention to this point of view and made experiment to examine the relationship between the alcohol extract of Korean rhei rhizoma and the motility of the isolated rabbit intestine, making the use of several drugs related to the motility of intestine such as acetylcholine, pilocarpine, atropine, papaverine, serotonin, and barium chloride. The movement of the isolated rabbit intestine in Tyrode's solution was recorded with the electric kymograph according to the Magnus method. The results of the experiment are as follows. 1. The motility of the isolated rabbit intestine represents the tendency of gradual dilatation in proportion to the concentration of R-A $10^{-4}$, $5{\times}10^{-4}$, and $10^{-3}$. 2. R-A $5{\times}10^{-4}$ does not seem to have the significant effect with acetylcholine, pilocarpine and atropine on motility of the isolated rabbit intestine strip. 3. R-A $5{\times}10^{-4}$ significantly blocks the contractile effect caused by serotonin $10^{-6}$ on motility of the isolated rabbit intestine strip. 4. R-A $5{\times}10^{-4}$ significantly blocks the contractile effect caused by barium chloride $10^{-4}$ on motility of the isolated rabbit intestine strip. 5. R-A $5{\times}10^{-4}$ significantly synergizes the dilatative effect caused by papaverine $10^{-6}$.

• 대한한의학방제학회지
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• 제24권3호
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• pp.163-174
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• 2016

Objectives : Rheum undulatum Linne and Glycyrriza uralensis Fischer are widely used herbal medicine. In this study, anti-oxidant and liver protective effects of R. undunlatum extract (RUE) and G. uralensis extract (GUE) were investigated in HepG2 cells, respectively. Oxidative stress and liver fibrosis were induced by arachidonic acid (AA) and iron, and CCl₄.Methods : MTT assay was assessed for cell viability, and immunoblotting analysis was performed to detect expression of apoptosis related proteins. In addition, reactive oxygen species (ROS) and mitochondrial dysfunction were measured. In vivo, BALB/c mouse were orally administrated with the aqueous extract of 10 mg/kg RUE and 100 mg/kg GUE for 3 days and then, injected with CCl₄ 0.5 ml/kg body weight to induce acute liver damage. Serum ALT level was measured, and histological change was observed in Harris's hematoxylin and eosin stainResults : RUE and GUE pre-treatment increased relative cell viability in concentration dependent manner and altered the expression levels of apoptosis-related proteins such as procaspase 3, PARP and Bcl-xL. RUE and GUE also inhibited the mitochondrial dysfunction and excessive reactive oxygen species (ROS) production induced by AA and iron. In addition, RUE and GUE activated liver kinase B1 (LKB1), by increasing phosphorylation. Moreover, RUE and GUE treatment decreased liver injuries induced by CCl₄, as evidenced by decreases in histological liver damage as well as serum alanine amino transferase (ALT) level.Conclusions : These data suggest that RUE and GUE has anti-oxidant and liver protective effects against AA and iron-induced oxidative stress and CCl₄-induced liver injury.

• 대한한의학원전학회지
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• 제25권1호
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• pp.117-139
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• 2012

Objective : In this study, I would like to emphasize the importance of context in studying quoted verses in "Donguisusebowon". Method : First, methodology applied in researches on "Donguisusebowon" quotations were organized. Next, in order to examine the effects of the aforementioned methodology, several quoted verses were approached in such method. Result : First, the verse, 'In cases of Dry-Feces(大便秘燥), Rheum undulatum Linne(大黃) could be used in the medicinal formulas and treatment, but not Croton tiglium Linne(巴豆)'. In the texts of Shanghanlun(傷寒論), Zhang, Zhongjing(張仲景) prescribed Sammulbaeksan(三物白散) in treating 'Gyeolhyung due to Excessive cold'(寒實結胸), which includes Croton tiglium Linne(巴豆). From this, we can see that Lee, jema's statement in "Donguisusebowon" is false. Conclusion : Lee, jema's mistake was that he accepted Shanghanlun(傷寒論) as quoted in "Donguibogam", instead of the original text, accidently quoting Heo, jun's source of reference.