• 환경생물
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• 제21권3호
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• pp.271-275
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• 2003

유기농업을 수행해온 배추와 무의 근권토양과 관행농법의 근권토양 및 비경작지 토양으로부터 토양시료를 채취하여 선충의 밀도를 조사하고, 토양내 유익선충인 곤충병원성 선충의 분포상황을 검토하였다. 경작지 토양 내 선충 분포율이 비경작지에 비하여 높았고, 유기농업 토양이 관행농업 토양보다 선충의 밀도가 높게 나타났다. 선충의 정량적 평가법으로 직접계수법과 MPN 법을 비교 조사한 결과 MPN법이 신속하고 정확한 방법임이 확인되었다. 각 토양으로부터 분리된 곤충병원성 선충은 Rhabditida목 선충과 Diplogasterida목 선충으로 분류되었다. 이들 선충 중 Diplogasterida목 선충이 50％ 이상을 차지하여 높은 점유율을 나타내었다. 누에를 기주체로 사용한 살충력 검정에 있어 24∼36시간 이내에 90％이상의 기주 치사율을 보여 농작물 해충방제를 위한 생물농약으로의 활용 가능성을 나타내었다.

• Journal of Species Research
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• 제8권3호
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• pp.259-267
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• 2019

Five free-living nematode species belonging to the order of Rhabditida are described for the first time in Korea. Two unrecorded species of Rhabditidae, Cruznema tripartitum (Linston, 1906) Zullini, 1982, and Poikilolaimus oxycerca de Man, 1895, and one species of Peloderidae, Pelodera strongyloides Schneider, 1860, were collected in Korea. C. tripartitum has nine pairs of genital papillae arranged in 2+2+1+4 differently from similar species of C. scarabaeum which has ten genital papillae arranged in 2+1+4+3. Poikilolaimus oxycerca has shorter tail (shorter than anal body diameter) compared to similar species of P. regenfussi which has longer tail(longer than anal body diameter). Pelodera strongyloides has shorter tail(1-1.5 times anal body diameters long) than P. punctata (4 anal body diameters long). Two unrecorded species of Panagrolaimidae, Panagrolaimus apicatus Schuurmans Stekhoven & Teunissen, 1938 and Panagrolaimus rigidus (Schneider, 1866) Thorne, 1937 were collected in Korea. P. apicatus has rounded and lower lip compared to similar species, P. margaretae Massey, 1964 which has finely pointed lip. P. rigidus has slender tail and lips rounded and flat barely separated when compared to similar species, P. subelongatus(Cobb, 1914) Thorne, 1937 which has plumper tail and lips are well separated.

• 환경생물
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• 제17권3호
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• pp.321-330
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• 1999

우리나라의 다양한 지역에서 100개의 토양과 곤충사체에서 누에 trap을 이용하여 곤충병원성 선충을 분리하였으며, 위생해충인 털검정파리유충(Calliphora vomitoria)과 농업해충인 멸강나방유충(Pseufaletia separata), 북방풀노린재 (Palomena angulosa) 그리고 왕풍뎅이유충(Melolontha incana) 에 대하여 20∼80%의 살충력을 나타내며, 누에(Bombyx mori mori)유충과 번데기에 대한 살충력은 100%인 선충 30계통을 선발하였다. 선발한 선충 30계통은 형태적 특징에 따라 Rhabditidae, Heteror-habditidae, Diplogasteridae, Steinernematidae, Tylenchidae의 5개 분류군으로 나뉘어졌다. 선충의 동정법 개선 및 분자생물학적 분류기준의 검토를 위하여 random primer 130개 중에서 20개의 primer를 선발하고, genomic DNA의 RAPD-RCR을 수행한 결과 유사도 0.853에서 형태적 분류기준과 일치하였다. 유사도계수를 이용한 5개 분류군에 속하는 선충 계통간의 유전적 근연관계는 Rhabditida에서 Steiner-nematidae는 경우 동일 목의 Heterorhabditidae및 Rhabditidae와는 유사도 0.819로서 거리가 멀고, 다른 목인 Tylenchidae와는 0.827로서 보다 더 근연이었다. 또한 Rhabditidae분류군은 유사도 0.863에서 다시 2개의 분류군으로 나누어졌으나 형태적 차이는 명확하지 않았다. 형태적 분류를 참조한 genomic DNA의 RAPD결과 유사도의 적절한 응용은 생물적 방제를 위한 곤충병원성 선충의 선발 및 동정에 있어서 형태적 단순성에 기인하는 자연분류의 한계를 보완 또는 극복할 수 있는 분자생물학적 동정법으로서 유용할 것이다. 또한 RAPD에 의한 동정은 생태환경에서 다수의 개체를 확보하기 쉬운 dauer larvae 또는 infective juvenile 등 유충기의 선충을 이용할 수 있다. 그러므로 노력이 소요되는 성충의 채집과 표본제작 등이 생략되므로 선충의 동정에 매우 안정적이며 효율적인 방법으로 판단되었다.

• Journal of Species Research
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• 제6권2호
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• pp.177-180
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• 2017

Diploscapter coronatus (Cobb, 1893) Cobb, 1913 was first reported on oriental melon (Cucumis melo vars. makuwa Makino) roots from plastic film houses, Gyeongsangbuk-do, Andong, Korea (latitude $N36^{\circ}33^{\prime}$. longitude $E128^{\circ}29^{\prime}$). Diploscapter coronatus is most similar to D. pachys. Species in the genus Diploscapter have a visibly annulated cuticle. The stoma of D. coronatus is 1.5 times longer than the lip region width. The pharyngeal corpus is clearly separated from the isthmus and the vulva is situated around the mid-body. However, it differed by higher ratio of "a", filiform tail and had relatively blunt labial hooks. The Korean population is well matched and within the range of D. coronatus as described from Iran and Ethiopia in de Man's of L, b, c, c', and V. Female length, however, varied between populations: the Ethiopia population female length is longer than in the Korea population (396.4 vs 427.0) while females in the Iran population are smaller than in the Korea population (396.4 vs 350.0). Nematode size may vary due to environmental conditions such as food sources. The position of excretory pores in the Korean population were shorter 53.9-72.5 than in other populations (67-82 and 70-89). Males were uncommon. Males in the Korean population are smaller than females but larger(356.0) than males in the Indian population (306.0).

• Animal Systematics, Evolution and Diversity
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• 제33권4호
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• pp.256-261
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• 2017

Eucephalobus oxyuroides (de Man, 1876) Steiner, 1936 belonging to the family Cephalobidae Filpijev, 1934 (Cephalobomorpha) is newly reported from South Korea. This species is distinguished from other Eucephalobus species by its pointed tail terminus, high lips and relatively longer female tail and body. From many previous studies, intraspecific variation in this species has been reported for body length, absence/presence of probolae, and number/position of papillae in the posterior region of male, posing an obstacle for identification and phylogenetic analysis. In this study, details of the morphological characters and morphometric of E. oxyuroides Korean population are described and illustrated based on optical microscopy.

• Animal Systematics, Evolution and Diversity
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• 제32권4호
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• pp.258-265
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• 2016

Acrobeloides nanus (de Man, 1880) Anderson, 1968 belonging to the family Cephalobidae Filpijev, 1934 (Cephalobomorpha) is newly reported from South Korea. This species is distinguished from other Acrobeloides species by its low and blunt labial probolae, five lateral incisures with middle incisure extending to the tail tip, and bluntly rounded tail. In this study, details of morphological characters of A. nanus is described and illustrated based on optical and scanning electron microscopy. In addition, molecular sequence data of the D2-D3 region of 28S rDNA, 18S rDNA and mitochondria DNA cox1 region from this species are provided as DNA barcode sequences.

• Animal Systematics, Evolution and Diversity
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• 제37권4호
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• pp.345-348
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• 2021

The genus Eucephalobus Steiner, 1936, belonging to the family Cephalobidae Filipjev, 1934 (Cephalobomorpha), represents a group of bacterial feeding nematodes. Although this group is cosmopolitan and species-rich, only two species have been reported from South Korea. Eucephalobus iaculocaudatus Bostrom and Holovachov, 2011 was collected from natural forest in Chungcheongbuk-do, South Korea. This species shows typical morphological characters of the genus Eucephalobus, including a bicornuate labial probolae, cephalic probolae absent and three lateral incisures. However, E. iaculocaudatus is distinguished from other Eucephalobus species by conoid tail with a harpoon-shape mucro in female and ragged mucro in male. In this study, detailed morphological characters and morphometrics of E. iaculocaudatus are described and illustrated based on optical microscopy.

• Animal Systematics, Evolution and Diversity
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• 제35권3호
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• pp.95-104
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• 2019

The family Mesorhabditidae $Andr{\acute{a}}ssy$, 1976 is the most abundant group in Rhabditoidea and occurs all over the world. Over 85 valid species have been reported from Mesorhabditidae; however, only 2 species have been recorded in South Korea. In this study, Bursilla vernalis $Andr{\acute{a}}ssy$, 1982, Mesorhabditis inarimensis (Meyl, 1953) Dougherty, 1955, and M. minuta $Bostr{\ddot{o}}m$, 1991 belonging to the family Mesorhabditidae (Rhabditomorpha), are newly reported from South Korea. Species in this family are distinguished from other rhabditomorphs by a posteriorly located vulva and an unpaired prevulval female gonad. Details of the morphological characters and morphometrics of B. vernalis, M. inarimensis, and M. minuta are described and illustrated based on optical and/or scanning electron microscopy.

• Animal Systematics, Evolution and Diversity
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• 제35권2호
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• pp.41-48
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• 2019

Pseudacrobeles(Pseudacrobeles) variabilis(Steiner, 1936) Steiner, 1938 and P. (Bunobus) pseudolatus($Hern{\acute{a}}ndez$, 1990) De Ley, Siddiqi and $Bostr{\ddot{o}}m$, 1993 belonging to the family Cephalobidae Filipjev, 1934 are newly reported from South Korea. Pseudacrobeles (Pseudacrobeles) variabilis is distinguished from its congeners by having distinctly setiform cephalic probolae, three rounded or knob-shaped labial probolae and longer female tail. Pseudacrobeles (Bunobus) pseudolatus is distinguished from its congeners by having visibly lateral lips, a short post-uterine sac, elongated conoid tail in the female, and acute mucro on the tails of both sexes. In this study, details of the morphological characters and morphometrics of Korean populations of P. (P.) variabilis and P. (B.) pseudolatus are described and illustrated based on optical microscopy.

• Journal of Species Research
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• 제10권4호
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• pp.405-411
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• 2021

The genus Acrobeloides(Cobb, 1924) Thorne, 1937 are bacterial feeders and are one of the most abundant and widely distributed nematode groups in various terrestrial environments. Based on morphological and morphometric analyses, we found two Acrobeloides species reported in Korea for the first time: A. bodenheimeri (Steiner, 1936) Thorne, 1937 and A. tricornis (Throne, 1925) Thorne, 1937. These species exhibit morphological characters concordant with typical features of the genus Acrobeloides, such as a fusiform pharyngeal corpus with swollen metacorpus and lateral incisures extending to the tail terminus. However, A. bodenheimeri is distinguished from other acrobeloids by having its low and rounded labial probolae, distinct post-uterine sac and five lateral incisures. Acrobeloides tricornis is distinguished from its congeners by the following characteristics: its high labial probolae with acuate termini, inconspicuous post-uterine sac and five lateral incisures. Morphological characters and their measurements, and illustrations of A. bodenheimeri and A. tricornis are described in this study.