• Journal of Ginseng Research
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• v.39 no.4
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• pp.384-391
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• 2015

It has been reported that Korean Red Ginseng has been manufactured for 1,123 y as described in the GoRyeoDoGyeong record. The Korean Red Ginseng manufactured by the traditional preparation method has its own chemical component characteristics. The ginsenoside content of the red ginseng is shown as Rg1: 3.3 mg/g, Re: 2.0 mg/g, Rb1: 5.8 mg/g, Rc:1.7 mg/g, Rb2: 2.3 mg/g, and Rd: 0.4 mg/g, respectively. It is known that Korean ginseng generally consists of the main root and the lateral or fine roots at a ratio of about 75:25. Therefore, the red ginseng extract is prepared by using this same ratio of the main root and lateral or fine roots and processed by the historical traditional medicine prescription. The red ginseng extract is prepared through a water extraction ($90^{\circ}C$ for 14-16 h) and concentration process (until its final concentration is 70-73 Brix at $50-60^{\circ}C$). The ginsenoside contents of the red ginseng extract are shown as Rg1: 1.3 mg/g, Re: 1.3 mg/g, Rb1: 6.4 mg/g, Rc:2.5 mg/g, Rb2: 2.3 mg/g, and Rd: 0.9 mg/g, respectively. Arginine-fructose-glucose (AFG) is a specific amino-sugar that can be produced by chemical reaction of the process when the fresh ginseng is converted to red ginseng. The content of AFG is 1.0-1.5% in red ginseng. Acidic polysaccharide, which has been known as an immune activator, is at levels of 4.5-7.5% in red ginseng. Therefore, we recommended that the chemical profiles of Korean Red Ginseng made through the defined traditional method should be well preserved and it has had its own chemical characteristics since its traditional development.

• Journal of Life Science
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• v.17 no.10
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• pp.1426-1433
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• 2007

To investigate the antibiotic resistant patterns of the bacteria producing ESBL, we isolated one organism of Klebsiella pneumoniae from a clinical laboratory in Busan. The organism that produces ESBL gene was detected by double disk synergy test and the presence of three ESBL genes (TEM-1, SHV-12, CTX-M-15) was confirmed by polymerase chain reaction and DNA sequencing analysis. To analyse the characteristics of three ESBL genes, we performed transconjugation, transformation and cloning experiment with the organism. The MIC of Klebsiella pneumoniae was revealed that ceftazidime, cefotaxime and ceftriaxone were $256\;{\mu}g/ml,\;128\;{\mu}g/ml\;and\;128\;{\mu}g/ml$ respectively. The MIC of conjugant (E. coli $RG176^{Na(r)}$) af was revealed that ceftazidime, cefotaxime and ceftriaxone were $256\;{\mu}g/ml,\;64\;{\mu}g/ml\;and\;128\;{\mu}g/ml$ respectively. The MIC of transformant (E. cofi $DH5{\alpha}$) was revealed that ceftazidime, cefotaxime and ceftriaxone were $128\;{\mu}g/ml,\;32\;{\mu}g/ml,\;and\;32\;{\mu}g/ml$ respectively, The MIC of cloned organism of SHV-12 gene (E. coli $DH5{\alpha}$) was revealed that ceftazidime, cefotaxime and ceftriaxone were $128\;{\mu}g/ml,\;8\;{\mu}g/ml,\;and\;32\;{\mu}g/ml$ respectively. The results indicated that MIC of conjugant was higher than MIC of transformant and also SHV-12 gene were not resistant against cefotaxime antibiotic.

• Journal of the Korea institute for structural maintenance and inspection
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• v.24 no.5
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• pp.61-68
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• 2020

In this study, the international standard ISO TS 16774 Part 3 Test Method for Water (wash out) Resistance and KS F 4935 ｢Sealant Injection type for water leakage maintenance of adhesive flexible rubber asphalt series｣, which are standardized as a quality control method of injection type repair materials used for water leakage cracks in underground concrete structures, are currently used in Korea. As a result, considering the performance criteria of "mass change rate -0.1%" stipulated in KS F 4935, the remaining 13 types repair materials, excluding RG-2 of synthetic rubber and UG-1 of urethane, need to be reviewed for stabilization of the loss resistance due to the flow of ground water. The results of this study are determined to be available as a basic indicator for the selection of repair materials used for cracks in concrete structures. In addition, it is expected that the results of this study can be utilized as reference data that can be reflected in the improvement of the quality of repair materials that will be researched and developed later.

• Korean Journal of Food Science and Technology
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• v.50 no.4
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• pp.383-390
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• 2018

Most neurodegenerative diseases are known to be influenced by oxidative stress. We investigated the anti-oxidative activity of the concentrate of fermented wild ginseng root culture (HLJG0701) containing ginsenosides Rg5 and Rk1. HLJG0701 showed effective DPPH and ABTS radical scavenging ability ($IC_{50}$: 16- and 4-fold dilution, respectively) and was inhibited dose-dependently by the $FeSO_4$-induced lipid peroxidation group (8- and 4-fold dilution: 2.3 and 1.5 nM, respectively). In MTT and LDH assays, 8-, 16-, 32- and 64-fold diluted HLJG0701 significantly increased cell viability by 70, 53, 35, and 26%, respectively. LDH released by HLJG0701 was reduced 1.3-fold with 8-fold diluted HLJG0701 compared to the $H_2O_2$-treated control. In addition, the inhibitory effect of HLJG0701 on oxidative stress in PC12 cells was confirmed by DCF-DA analysis (16-, 4-fold diluted HLJG0701: 50 and 68% ROS inhibition, respectively), TBARS (16- and 4-fold diluted HLJG0701: 50.7 and 46.5% inhibition, respectively), GPx (16- and 4-fold diluted HLJG0701: 133.3 and 227.3% release, respectively), and SOD analysis (16- and 4-fold diluted HLJG0701: 118.2 and 218.2% release, respectively). These results suggested that HLJG0701 protects neuronal cells by its anti-oxidative effects and hence can be a potential preventive material against neurodegenerative diseases.

• Applied Biological Chemistry
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• v.20 no.2
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• pp.188-204
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• 1977

The studies on the saponins of Korean ginseng, Panax ginseng C.A. Meyer, were performed according to the cultivating locations, sampling seasons, plant parts, and growing stages. The changes in saponin content in the course of manufacturing Red ginseng and Ginseng extract were observed. In this paper, a new method for the determination of the total and the individual saponin glucosides was proposed and applied to the samples under study. The method employing Digital Densitorol DMU-33C (Toyo electric Co., Japan) followed the separation of the saponins by means of a preparative thin layer chromatography. The saponin contents and their fractional distribution were summarized as follows: 1. The average concentrations(% plant dry weight) of semi-purified saponins in the roots of Korean ginseng planted in the various locations were 5.0%(Keumsan), 6.0% (Kimpo), and 5.4% (Pocheon), respectively. 2. There were 3.3% saponins in White ginseng(Rhizome) and 12.7% saponins in Ginseng tail (Fibrous root). 3. Regarding the year of growth, the contents of saponins were 90.3mg (2-year-old ginseng), 254.4mg (3-year-old ginseng), 404.2mg (4-year-old ginseng). 999.6mg (5-year-old ginseng), and 1377.1mg (6-year-old ginseng) respectively, and the saponin factions containing panaxatriol as an aglycone increased. 4. Thin layer chromatography revealed that Red ginseng yielded many saponins which Shibata et al. designated as $ginsenoside-Rb_1$ (22.1%), $-Rb_2(15.4%)$, -Rc(12.6%), -Re (15.7%), and $-Rg_1$, (9.3%). 5. 29.9% of crude saponins were isolated from ethanolic extract of Panax ginseng fibrous root and their extraction yield was 94.2% of fibrous root saponin.

• Food Science and Preservation
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• v.6 no.3
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• pp.324-327
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• 1999

This study was carried out to establish the extraction method of red ginseng extract without saponin decomposition. Red ginseng was extracted with impulse vacuum system and multi-stage extraction method. Crude saponin content of red ginseng extract (RGE) from impulse vacuum system was 5.4-5.9%, while that of RGE from multi-stage extraction method was 8.2-8.3%. However, HPLC Patterns indicated that saponins of RGE from impulse vacuum system were hardly decomposed, while those of RGE from multi-stage extraction method were decomposed, especially in ginsenoside -Rgl and -Re saponin. Also, the yields of red ginseng by impulse vacuum system were 15 to 20 times higher than that of multi-stage extraction method.

• Journal of Ginseng Research
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• v.37 no.3
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• pp.349-354
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• 2013

Dried ginseng (DG) is in fact the representing ginseng product in the worldwide market. Although it is made in various packages depending on the processing method, size and age of DG, basic scientific data reporting the chemical components are limited. In this study, 4-year-old curved ginseng (CG), one of the domestic DG products, was selected for further investigation. Eighty-six samples of 30 and 50 piece-grade CG, which are the most widely distributed in the market, were collected for 5 yr. Their major components, such as moisture, total sugar, acidic polysaccharides, total phenolic compounds, and saponins, were analyzed to figure out the standard quality characteristics. The moisture content of all CG samples was less than 15%. The total water-soluble sugar contents were 22.9% to 47.8% and 23.2% to 49.5% in the 30 and 50 piece-grade CG, respectively. The acidic polysaccharide contents were 3.6% to 6.7% and 2.9% to 6.9% in the 30 and 50 piece-grade CG, respectively. The total phenolic compound content was 0.4% to 0.5% in CG, regardless of the piece-grade. The crude saponin content, which represents the active component of ginseng, was over 2% in all samples. In 30 piece-grade CG samples, the contents of major ginsenosides, Rb1, Rf, and Rg1, were 2.2 to 4.7 mg/g, 0.4 to 1.3 mg/g, and 1.6 to 4.0 mg/g, respectively. The ginsenoside contents in 50 piece-grade CG samples were 2.1 to 3.9 mg/g (Rb1), 0.5 to 1.2 mg/g (Rf), and 1.3 to 3.4 mg/g (Rg1). Overall, since there were relatively high standard deviation and coefficient of variation in all the chemical component contents that were assessed, we found some difficulties in showing the CG standard chemical component characteristics by average, standard deviation, and other statistical analysis factors.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.11 no.9
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• pp.3325-3329
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• 2010

This study evaluated anti-wrinkle effect using toner and lotion containing ginsenoside Rg3 enriched black ginseng in oriental medicine like Liriope platy phyllla, Inonotus obliquus, Glycyrrhiza glabra and nourishing of the blood and energy(Atractylodes japonica, Poria cocos Wolf, Angelica gigas, Rehmanniae Radix Preparata, Cnidii Rhizoma, Paeonia lactiflora). 6 female adults on average at the age of 42.5 applied lotion after toner under the normal conditions of use on the face including crow's feed area, twice a day (in the morning and evening) for 4weeks. After 4 weeks, the replica of eye wrinkle was made and analyzed the image. The result was decreased in R1($3.15{\pm}0.35$ to $2.28{\pm}0.22$), R2($2.99{\pm}0.37$ to $2.13{\pm}0.21$), R3($2.09{\pm}0.31$ to $1.56{\pm}0.18$), R4($0.37{\pm}0.05$ to $0.29{\pm}0.03$), and R5($0.89{\pm}0.1$ to $0.75{\pm}0.06$)(P<0.05). Therefore, oriental medicine cosmetics containing black ginseng may play a role anti-wrinkle.

• Journal of Cadastre & Land InformatiX
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• v.47 no.1
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• pp.15-26
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• 2017

The decrease of green space according to the urbanization has caused many environmental problems as the destruction of habitat, air pollution, heat island effect. With interest growing in natural view recently, proper management of evergreen tree which is lived even the winter season has been on the rise importantly. This study analyzed the distribution area of evergreen tree using vegetation index based on unmanned aerial vehicle (UAV). Firstly, RGB and NIR+RG camera were loaded in fixed-wing UAV and image mosaic was achieved using GCPs based on Pix4d SW. And normalized differences vegetation index (NDVI) and soil adjusted vegetation index (SAVI) was calculated by band math function from acquired ortho mosaic image. validation points were applied to evaluate accuracy of the distribution of evergreen tree for each range value and analysis showed that kappa coefficient marked the highest as 0.822 and 0.816 respectively in "NDVI > 0.5" and "SAVI > 0.7". The area of evergreen tree in "NDVI > 0.5" and "SAVI > 0.7" was $11,824m^2$ and $15,648m^2$ respectively, that was ratio of 4.8% and 6.3% compared to total area. It was judged that UAV could supply the latest and high resolution information to vegetation works as urban environment, air pollution, climate change, and heat island effect.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.55 no.4
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• pp.299-305
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• 2010

In this study, the contents of ginsenoside were compared according to the red ginseng extract times to provide basic information for developing nutraceutical foods using red ginseng. The highest total ginsenoside contents of the main, lateral, and fine root extracts were 23.04, 65.68, and 295.92 mg/100 mL when extracted at $75^{\circ}C$ for 21, 18, and 12 hours, respectively. The total ginsenoside content showed a tendency to decrease as the extraction times were increased. The highest Rb1 and Rg1 contents of the main, lateral, and fine root extracts were 5.76, 28.39, and 117.83 mg/100 mL when extracted at $75^{\circ}C$ for 18, 15, and, 12 hours, respectively, and their highest Rb2 and Re contents were 5.76, 28.39, and 117.83 when extracted under the same conditions. The prosapogenin content of the red ginseng extract increased along with the extraction time. The highest total ginsenoside extraction ratios of the main, lateral, and fine root extracts of the red ginseng at $75^{\circ}C$ were 21.3, 21.1, and 67.1%, respectively.