• Journal of Ginseng Research
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• 제23권1호
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• pp.55-59
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• 1999

For the first time we have developed a simple method for conjugation between ginsenoside Rf (Rf) and bovine serum albumin (BSA) or ovalbumin (OVA) by a periodate oxidation method. The Rf-BSA conjugate is confirmed by ultraviolate (UV) scanning, thin layer chromatography (TLC), and SDS-gel electrophoresis. In UV scanning Rf showed three small peaks approximately at 230, 265 and 280 nm. BSA showed a peak at 280 nm. The Rf-BSA conjugate showed right shifted three peaks that BSA alone did not show. In TLC analysis, the Rf-BSA conjugate did not show mobility in silica gel but showed a slight stream of trace. In SDS-gel electrophoresis, Rf-BSA conjugate show a slight less mobility than BSA alone. Rf-OVA conjugate also showed similar patterns with Rf-BSA conjugate. These results demonstrated that periodate oxidation method could be used to produce a stable Rf-BSA or Rf-OV A conjugate and also could be applied for other ginsenoside(s).

• 대한수의학회지
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• 제26권2호
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• pp.225-228
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• 1986

Progesterone immunogen has been synthesized and its melting point, Rf-value, UV and IR spectrum have been measured to develope the essential step in antisera production against low molecular weight substance. Mixed anhydride reaction was used to conjugate $11{\alpha}$-hydroxy-progesterone with succinic anhydride. Melting point of one intermediate compound was $156^{\circ}C$, and Rf-value was 0.41 in benzene : acetone : methanol (5 : 5 : 2). Maximum absorbance was measured at 242nm and ${\varepsilon}$ was $1.641{\times}10^4cm^2/mole$. Loss of hydroxy group was observed at 3450nm, and carbonyl group was appeared at 1160nm, 1250nm and 2960nm. These results indicated that the intermediate compound was progesterone hemisuccinate. Maximum absorbance of progesterone bovine-serum albumin(BSA) conjugate was observed at 250nm. Molar ratio of progesterone to BSA was average 15.4 on UV spectrum.