• 멤브레인
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• 제27권3호
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• pp.205-215
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• 2017

고분자 분리막은 가격이 저렴하고, 쉽게 제조가 가능하며, 투과도와 선택도가 우수하여 수처리 분야뿐만 아니라 기체분리에서도 중요한 역할을 한다. 하지만, 고분자 분리막은 일반적으로 투과도와 선택도의 역상관 관계를 나타내는 단점이 있다; 즉, 투과도가 높으면 선택도가 낮고, 선택도가 높으면 투과도가 높다. 이러한 단점을 극복하기 위한 방안 중의 하나가 촉진수송이다. 지난 수십 년간 촉진수송 이론은 촉진수송 분리막 제조에 있어 매우 중요하고 다양한 모델을 제시하는 데에 핵심적인 역할을 하였다. 한편, 촉진수송에서 주된 역할을 하는 운반체의 특성, 매질의 유동성 및 고분자 복합체의 물리화학적 성질 등을 이해하는 것은 중요하다. 운반체의 유동성에 따라 촉진수송 분리막의 종류를 3가지로 나눌 수 있다; 즉, 이동상 운반체 분리막, 준이동상 운반체 분리막, 고정상 운반체 분리막. 또한 촉진 운반체가 특정물질과 상호작용하는 데에는 4가지 종류의 가역반응으로 나눌 수 있다; 즉, 수소원자 전달 반응, 친핵성 첨가반응, 파이-착체 반응, 그리고 전기화학 반응. 이러한 촉진수송 분리막은 이산화탄소, 산소, 올레핀(프로필렌, 에틸렌)의 투과도를 선택적으로 향상시키는 역할을 한다. 이와 같이 본 총설에서는 다양한 촉진수송 분리막에 관련된 주요 연구내용과 이러한 연구를 수행하는 대표적인 전략들을 소개하고자 한다.

• Korean Chemical Engineering Research
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• 제60권2호
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• pp.217-222
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• 2022

고분자 전해질 연료전지(PEMFC) 고분자 막의 전기화학적 내구성을 가속적으로 평가하는 개회로 전위 유지(OCV holding) 과정에서 OCV 변화 거동을 해석하는 것은 매우 중요하다. 본 연구에서는 내구성이 각기 다른 세 종류의 MEA(membrane electrode assembly)의 실험데이터를 이용한 실험식을 만들어 비교 및 검토하였다. 막 내부에 라디칼 제거제가 없는 강화막 MEA의 내구 평가시간은 383 h, 막 내부에 라디칼 제거제가 있는 강화막 MEA의 내구 평가시간은 각각 1,000, 1,650 h이었다. 고분자 막의 열화는 활성화에 의해 회복이 가능한 가역적 열화와 회복이 되지 않은 비가역적 열화로 구분했다. 고분자 막의 비가역적 열화는 수소투과도 증가로 나타나는데 수소투과도 변화가 세 MEA 모두 비가역적 열화 상수 c와 유사한 형태를 보였다. 회복이 되지 않은 비가역적 열화가 시작되는 것은 수소투과도 증가로 나타나고, 수소투과도 증가로 인해 OCV가 회복되지 않아서 OCV 회복선의 기울기가 감소하고 이를 실험식의 상수 c 값의 증가로 확인할 수 있었다.

• 한국소음진동공학회논문집
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• 제21권12호
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• pp.1132-1137
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• 2011

MRE(magneto-rheological elastomers) is a material which shows reversible and various modulus in magnetic field. Comparing to conventional rubber vibration isolator, MREs are able to absorb vibration of broader frequency range. These characteristic phenomena result from the orientation of magnetic particles named carbonyl iron powder(CIP) in rubber matrix. In this paper, simulation on variation rate of shear modulus for anisotropic MRE due to volume fraction of CIP and an effective permeability model was applied to predict the field-induced shear modulus of MREs. Also, the variation rate of shear modulus for anisotropic MRE was derived using magneto-mechanical theory. Based on Maxwell-Garnett mixing rule, the increment of shear modulus was calculated to evaluate the shear modulus of MREs with column structure of CIP due to induced current. The simulation results on variation rate of shear modulus can be applied to the variable mechanical system of MRE such as tunable vibration absorber, stiffness variable bush and mount.

• International Journal of Oral Biology
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• 제37권1호
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• pp.17-23
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• 2012

Recent studies indicate that reactive oxygen species (ROS) are critically involved in persistent pain primarily through spinal mechanisms, and that mitochondria are the main source of ROS in the spinal dorsal horn. To investigate whether mitochondrial ROS can induce changes in membrane excitability on spinal substantia gelatonosa (SG) neurons, we examined the effects of mitochondrial electron transport complex (ETC) substrates and inhibitors on the membrane potential of SG neurons in spinal slices. Application of ETC inhibitors, rotenone or antimycin A, resulted in a slowly developing and slight membrane depolarization in SG neurons. Also, application of both malate, a complex I substrate, and succinate, a complex II substrate, caused reversible membrane depolarization and enhanced firing activity. Changes in membrane potential after malate exposure were more prominent than succinate exposure. When slices were pretreated with ROS scavengers such as phenyl-N-tert-buthylnitrone (PBN), catalase and 4- hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPOL), malate-induced depolarization was significantly decreased. Intracellular calcium above $100{\mu}M$ increased malateinduced depolarization, witch was suppressed by cyclosporin A, a mitochondrial permeability transition (MPT) inhibitor. These results suggest that enhanced production of spinal mitochondrial ROS can induce nociception through central sensitization.

• Advances in Traditional Medicine
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• 제5권3호
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• pp.240-245
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• 2005

The effect of aqueous and alcoholic extract of seeds of Nigella sativa (N. sativa) was studied on the spontaneous movements of the whole worm (w.w) preparation and nerve muscle (n.m) complex of Setaria cervi (S. cervi). Both the extracts caused inhibition of spontaneous movements of the w.w and n.m complex characterized by initial stimulation followed by irreversible paralysis, with the exception that aqueous extract produced a partially reversible paralysis of w.w preparation. Lesser concentration of both the extracts was required to inhibit the movements of n.m complex than the w.w. Suggesting a cuticular permeability barrier. The lethal concentration $50\;(LC_{50})$ and lethal concentration $90\;(LC_{90})$ were 30 and 55 ng/ml for aqueous and 45 and 60 ng/ml for alcoholic extracts respectively.

• Advances in Traditional Medicine
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• 제4권1호
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• pp.53-59
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• 2004

The effect of aqueous and alcoholic extract of the leaves of Ocimum. sanctum was studied on the spontaneous movements of the whole worm (w.w) preparation and nerve muscle (n.m.) complex of Setaria cervi (S. cervi) and on the survival of microfilariae (m.f.) in vitro. Both the extracts caused inhibition of the spontaneous motility of the w.w. and n.m. complex of S. cervi characterized by initial stimulation followed by reversible paralysis, aqueous extract at a higher concentration showed immediate effect and irreversible paralysis. The concentration required to inhibit the movements of n.m. complex was $1/4^{th}$ for aqueous and $1/3^{rd}$ for alcoholic extract compared to that for the w.w., suggesting a cuticular permeability barrier. On the m.f. the lethal concentration (LC 50 and LC 90) were 35 and 50 ng/ml for aqueous whereas, 60 and 85 ng/ml for alcoholic extracts respectively.

• Journal of Pharmaceutical Investigation
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• 제37권4호
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• pp.237-242
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• 2007

The chemical formula of indapamide is 3-(aminosulfonyl)-4-chloro-N-(2,3-dihydro-2-methyl-1H-indol-l-yl)-benzamide, Indapamide is an oral antipertensive diuretic agent indicated for the treatment of hypertensive and edema. Indapamide inhibits carbonic anhydrase enzyme. Transdermal drug delivery systems, as compared to their corresponding classical oral or injectable dosage form counterparts, offer many advantages. The most important advantages are improved systemic bioavailability of the pharmaceutical active ingredients (PAI), because the first-pass metabolism by the liver and digestive system are avoided; and the controlled, constant drug delivery profile (that is, controlled zero-order absorption). Also of importance is the reduced dose frequency compared to the conventional oral dosage forms (that is, once-a-day, twice-a-week or once-a-week). Other benefits include longer duration of therapeutic action from a single application, and reversible action. For example, patches can be removed to reverse any adverse effects that may be caused by overdosing. In order to evaluate the effects of vehicles and penetration enhancers on skin permeation of Indapamide, the skin permeation rates of Indapamide from vehicles of different composition were determined using Franz cells fitted with excised hairless skins. Solubility of Indapamide in various solvents was investigated to select a vehicle suitable for the percutaneous absorption of Indapamide, The solvents used were Tween80, Tween20, Labrasol, Lauroglycol90 (LG90) and Peceol. Lauroglycol90 increase the permeability of indapamide approximately 3.75-fold compared with the control. Tween80, Tween20, Labrasol, Lauroglycol90 (LG90) and Peceol showed flux of $0.06ug/cm^2/hr,\;0.4ug/cm^2/hr,\;0.21ug/cm^2/hr,\;0.72ug/cm^2/hr,\;0.29ug/cm^2/hr$, respectively.

• Advances in Traditional Medicine
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• 제3권4호
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• pp.180-186
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• 2003

Effect of aqueous and alcoholic extracts of the roots of Mirabilis jalapa Linn. Four Oclock plant, on the spontaneous movements of both the whole worm and the nerve-muscle (n.m.) preparation of Setaria cervi and on the survival of microfilariae in vitro was studied. Alcoholic extract caused the inhibition of spontaneous movements of the whole worm and the n.m. preparation of S. cervi, whereas aqueous extract caused inhibition of spontaneous movements of the n.m. preparation. The initial stimulatory effect was not observed by aqueous and alcoholic extracts on n.m. preparation while effect of alcoholic extract on the whole worm was characterized by an increase in the amplitude of contractions followed by reversible paralysis. The concentrations required to inhibit the movements of the whole worm and n.m. preparation for alcoholic extract of root were $270\;{\mu}g/mL$ and $40\;{\mu}g/mL$, respectively whereas an aqueous extract caused inhibition of n.m. preparation at $30\;{\mu}g/mL$ suggesting a cuticular permeability barrier. Alcoholic extract of the roots of M. jalapa caused concentration related effect on the survival of microfilariae of S. cervi. The $LC_{50}$ and $LC_{90}$ for alcoholic extract as observed after 6 hrs. were found to be 10 ng/mL and 18 ng/mL., respectively.

• Asian-Australasian Journal of Animal Sciences
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• 제27권2호
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• pp.266-277
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• 2014

Somatic cell nuclear transfer (SCNT) has generally demonstrated that a differentiated cell can convert into a undifferentiated or pluripotent state. In the SCNT experiment, nuclear reprogramming is induced by exposure of introduced donor nuclei to the recipient cytoplasm of matured oocytes. However, because the efficiency of SCNT still remains low, a combination of SCNT technique with the ex-ovo method may improve the normal development of SCNT embryos. Here we hypothesized that treatment of somatic cells with extracts prepared from the germinal vesicle (GV) stage Siberian sturgeon oocytes prior to their use as nuclear donor for SCNT would improve in vitro development. A reversible permeability protocol with $4{\mu}g/mL$ of digitonin for 2 min at $4^{\circ}C$ in order to deliver Siberian sturgeon oocyte extract (SOE) to porcine fetal fibroblasts (PFFs) was carried out. As results, the intensity of H3K9ac staining in PFFs following treatment of SOE for 7 h at $18^{\circ}C$ was significantly increased but the intensity of H3K9me3 staining in PFFs was significantly decreased as compared with the control (p<0.05). Additionally, the level of histone acetylation in SCNT embryos at the zygote stage was significantly increased when reconstructed using SOE-treated cells (p<0.05), similar to that of IVF embryos at the zygote stage. The number of apoptotic cells was significantly decreased and pluripotency markers (Nanog, Oct4 and Sox2) were highly expressed in the blastocyst stage of SCNT embryos reconstructed using SOE-treated cells as nuclear donor (p<0.05). And there was observed a better development to the blastocyst stage in the SOE-treated group (p<0.05). Our results suggested that pre-treatment of cells with SOE could improve epigenetic reprogramming and the quality of porcine SCNT embryos.

• 한국축산시설환경학회지
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• 제6권3호
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• pp.175-184
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• 2000

Lab-scale의 실험을 통하여 축산폐수의 액비화에 있어서 막 분리 기술의 도입에 따른 막 오염 특성과 투과특성에 대해 살펴본 결과 다음과 같은 결론을 얻을 수 있었다. 1. System의 안정적 운전을 위해서는 낮은 투과유속에서의 운전이 효과적이라 사료되며, Tmax가 지수 함수적으로 투과유속에 대해 감소하였다. 이를 통해서 예측식을 수립하여 운전에 있어서의 막 세정 시기를 결정할 수 있을 것으로 사료된다. 2. 전반적인 막 자체에 대한 순수투과저항을 관찰해본 결과 막 오염은 비가역적 영역보다는 가역적 영역에 의해 이루어진다고 사료된다. 3. 투과유속이 낮을 경우 막면 부착물에 의한 여과저항의 상승이 막 오염의 주된 원인이 되는 반면, 투과유속이 증가할수록 농도분극현상이 막 오염의 주된 원인이 된다고 사료된다. 따라서 높은 투과유속에서의 운전을 위해서는 이러한 농도 분극현상을 최소화 하기 위해서는 이러한 농도 분극현상을 최소화 하기 위하여 높은 전단응력을 제공하거나 막표면에 물리적 충격을 주어 농도 분극현상을 제어하는 방법이 추가되어야 할 것이라 사료된다. 4. 중력여과 및 간헐여과를 통한 막 분리 특성을 살펴본 결과 중력여과의 경우 별도의 흡입압력을 부여하지 않고 단지 수위차에 의해 생성되는 위치에너지에 의한 여과에서도 1.7LMH에서 약 3.5일간 안정되게 유지된 것을 확인할 수 있었으며, 간헐여과를 통해 농도 분극현상을 감소시킴으로 해서 연속여과보다 안정된 운전이 이루어졌다. 종합적으로 고찰해 봤을 때, 축산폐수의 액비화에 있어서 막 분리 기술의 도입은 그 유출수의 성상에 있어서 원수의 성상에 상관없이 일정한 질을 보이고 있음을 확인할 수 있었고, 공정의 안정적 유지를 위해서는 낮은 투과유속의 흡입과 정지의 간격이 비교적 긴 간헐여과 방식이 적합하다고 사료되며, 높이차를 이용한 중력여과 또한 추가적인 연구가 진행된다면 동력의 소모면에서 이점이 있을 것이라 사료된다.기는 축사의 난방 보조열원으로서 양호한 열효율을 나타내어 우리나라 축사시설에 적용가능성을 제시하였다. 본 지열교환기 시스템은 지열을 이용하는 환경보전형 에너지 시스템으로서 시설하기 간편하고 경제적이며 반영구적인 냉난방 및 환기시스템이다. 따라서, 우리나라 축산시설에 실용화될 경우에 우수한 대체에너지원으로 에너지 절감효과에도 크게 기여하리라고 전망된다.ble amount of acid mucin, Most of the medium sized and small mucous cells contained neutral mucin and sialomucin, but a few mucous cells contained neutral mucin and strongly sulfomucin or neutral combined with strongly sulfomucin and sialomucin. Most of the esophageal mucous cells pf Bryzoichthys lysimus contained small amount of neutral mucin, while on the other hand a feww mucous cells contained small amount of neutral mucin and minimal amount of sialomucin. But the esophageal mucous cells of Takifugu pardalis contained considerable amount of neutral mucin only.분해가 더욱 촉진되었으며, 30℃에서 교반 처리를 행한 경우가 10℃에서 교반 처리를 행한 경우 보다 지방분해가 더욱 촉진되었다. 산양유 원유는 30℃에서 교반 처리 시간이 연장되어도 지방분해는 뚜렷한 증가를 나타내지 않았다.와 표준체중군 여자에게서 가장 높게 나타났다. 5. 남자의 53.9%, 여자의 83.2%가 체중 조절에 관심이 있다