• 생약학회지
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• 제24권4호
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• pp.309-312
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• 1993

A new method for separation and quantitative determination of isoflavone glucosides in rhizomes of Belamcanda chinensis (Iridaceae) by high performance liquid chromatography was elaborated. A reverse-phase system with a Spheri-5 RP-18 column using MeOH:HOAc:$H_2O$(24 : 5 : 71) as a mobile phase was developed. The isoflavonoids were detected at 268 nm and the analysis was successfully carried out within 15 min.

• Journal of Pharmaceutical Investigation
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• 제23권3호
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• pp.133-137
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• 1993

A high-performance liquid chromatographic assay using ion-pair reverse-phase system was developed for the separation of acebutolol and acebutolol acetyl metabolite in plasma. A ion-pair reversephase system consisting of an ODS-bonded silica column and a mixture of 20% $CH_3CN$, 0.1% $H_3PO_4$, 0.035 M heptanesulfonic acid and 0.005 M tetrabutylammonium hydrogen sulfate as the mobile phase were used. Triamterene was employed as an internal standard. Based on 0.2 ml of plasma, the detection limits were 10.4 ng/ml for acebutolol and 10.3 ng/ml of acebutolol acetyl metabolite at the signal-to-noise ratio of 3:1.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.253.3-254
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• 2002

In boiling aqueous solution, D-amygdalin usually begins to convert into neoamygdalin in 3 min and more than 30% of the initial D-amygdalin is found as neoamygdalin after 30 min. In this report, we establish methods for simple HPLC analysis and the inhibition of D-amygdalin conversion. D-Amygdalin and its conversion product, neoamygdalin, were clearly separated on reverse-phase column chromatography by an optimized eluent of 10mM sodium phosphate buffer (pH 3.8) containing 6% acetonitrile. (omitted)

• 한국자원식물학회지
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• 제20권3호
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• pp.263-266
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• 2007

High performance liquid chromatography (HPLC) was used for the analysis of chiisanoside in each stem and root of Acanthopanax senticosus collected from South Korea, North Korea, China and Russia. A reverse-phase system using a gradient of H$_{2}$O and acetonitrile as the mobile phase was developed and detection was at 210nm. The analysis was successfully carried out within 30 min. Chiisanoside was measured in the stem and root of A. senticosus collected from various countries.

• 약학회지
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• 제41권4호
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• pp.414-420
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• 1997

A rapid and sensitive high performance liquid chromatographic method with UV detection for the analysis of polyamines(putrescine, spermidine, spermine) has been developed. The benzoyl chloride derivatives of putrescine, spermidine and spermine are separated on a mc-Bondapak C18 reverse-phase column with 50% methanol as the mobile phase. This method was applied to the analysis of polyamines in the serum of normal human and uterine cancer patients. The results show that the mean level of polyamines in cancer patients serum is much higher than that in normal human serum.

• Natural Product Sciences
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• 제24권4호
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• pp.288-292
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• 2018

High-performance countercurrent chromatography (HPCCC) coupled with reversed-phase highperformance liquid chromatography (RP-HPLC) method was developed to isolate dihydrophaseic acid 3'-O-${\beta}$-D-glucopyranoside (DHPAG) from the extract of Nelumbo nucifera seeds. Enriched DHPAG sample (2.3 g) was separated by HPCCC using ethyl acetate/n-butanol/water system (6:4:10, v/v/v, normal-phase mode, flow rate: 4.0 mL/min) to give 23.1 mg of DHPAG with purity of 88.7%. Further preparative RP-HPLC experiment gave pure DHPAG (16.3 mg, purity > 98%). The current study demonstrates that utilization of CCC method maximizes the isolation efficiency compared with that of solid-based conventional column chromatography.

• 생약학회지
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• 제43권3호
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• pp.250-256
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• 2012

In order to extract the curcuminoid such as curcumin, demethoxycurcumin (DMC), and bisdemethoxycurcumin (BDMC) in turmeric (Curcuma longa), solvent extraction methods (dipping and ultrasonic extraction method) and solid-phase extraction (SPE) were used. RP-HPLC (reverse-phase high-performance liquid chromatography) and TLC (thin-layer chromatography) were used for identification and analysis the three curcuminoid. From the experimental results, it is evident that the percentage of curcuminoid extracted from turmeric by ultrasonic extraction method was higher than dipping method. The percentage of curcumin extracted from turmeric by pure methanol was higher than any aqueous methanolic composition. Moreover, the total peak area of three curcuminoid was above 92% in RP-HPLC using solid-phase extraction. These results will form a database for investigating the constituents of natural products and the resources of pharmaceutical, nutrition, and cosmetic products.

• Journal of Microbiology and Biotechnology
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• 제10권2호
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• pp.233-237
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• 2000

As part of a continuing investigation to identify free radical scavengers from the fruit bodies of basidiomycetes, we isolated two p-terphenyl compounds, designated as PAl and PA2l, from cethanolic extract of the fruit body of Pasillus panuoides. The methanolic extract was processed by ethyl acetate extraction and silica gel column chromatography to yield two active fraction. PAl was obtained from one of the fractions through Sephadex LH-20 and silica gel column chromatographies and reverse-phase HPLC. The other fraction was purified by Sephadex LH-20 and reverse-phase column chromatographies to produce PA2. The compounds PA1 and PA2 were identified as leucomentin-4 and leucomentin-2, respectively, on the basis of various spectroscopic analyses. These compounds exhibited strong inhibitory activities against lipid peroxidation in rat liver microsomes with IC_{50}$ values of 0.10 and $0.06{\;}\mu\textrm{g}ml$, respectively.

• 분석과학
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• 제17권2호
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• pp.98-107
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• 2004

본 연구에서는 $^1H$ NMR 분광분석법 및 HPLC를 이용하여 자동차 부품에 사용된 아크릴 코팅의 성분 및 함량을 분석하였다. $^1H$ NMR 분석 결과 아크릴 코팅은 88.40 wt%의 PMMA, 7.05 wt%의 MMA 및 2.36 wt%의 allyl methacrylate를 포함하고 있다. 또한, 고분자 첨가제로 벤조트리아졸 (Hisorb 328) 및 oxanilide 광안정제, BHT 및 DMP가 $^1H$ NMR 스펙트럼에서 확인되었고 정량적으로 측정되었다. 그러나, Bondapak C18 칼럼, 메탄올 이동상, PDA 검출기를 사용한 역상 HPLC 분석을 통해서는 두개의 광안정제만이 확인되었다. 두 광안정제의 함량은 UV-Vis 흡광광도계를 이용한 정량분석법을 통해 측정되고 NMR 분석 결과와 비교하였다. $^1H$ NMR 스펙트럼으로부터 얻은 분석 정보들이 HPLC에서 얻는 것들 보다 우수하였다.

• Journal of Nutrition and Health
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• 제24권3호
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• pp.199-205
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• 1991

비타민 A의 활성 대사물인 비타민 A arid(retinoir acid, Rh)를 사람의 간 조직내에서 처음으로 검출하고, 측정하는 방법을 고안하였다. Reverse phase HPLC에 gradient elution을 사용하는 이 방법은 Multiwavelength 검출법을 이용하여 RA, 비타민 A, 비타민 E 및 카로티노이드를 모두 약 30mg정도의 시료로부터 검출할 수 있었다. HPLC 분석에 소요되는 시간은 시료당 20분으로 상당히 신속하며 또 예민하다. 간 조직내의 총비타민 A량에 대한 Rh의 비율은 비타민 A의 축적량에 관계없이 일정한 편으로 약 $2.4\pm0.2%로$ 나타났다. RA의 간 조직내의 검출은 RA가 비타민 A의 체내 대사산물임을 다시 한번 확인시켰으며, 그 함량에 대한 정보는 총비타민 A량과 함께 비타민 A 영양 상태 판정에 보다 좋은 지표가 될 것이다. 특히 간 조직의 needle biopsy가 필요한 질병 상태의 경우, 비타민 A의 기능적 영양 상태판정을 가능하게 하여 질병의 진단 및 처치에 도움이 되리라 사료된다.