• 한국응용약물학회:학술대회논문집
• /
• 한국응용약물학회 1995년도 춘계학술대회
• /
• pp.76-76
• /
• 1995

Virus-encoded HIV-1 reverse transcriptase (RTase) is one of the major targets for the development of drugs for HIV-1 since it is an essential enzyme-for the replication cycle of HIV-1. We cloned the entire reverse trancriptase gene into an inducible expression vector with tac promotor= RTase was stably overexpressed and induced by IPTG and the highly-expressed RTase was purified partially by use of DEAE cellulose and Mono Q column. The partially purified enzyme (663kDa, 51kDa) as exhibited by SDS-PAGE showed the high specific activity (16,570U/mg) when the assay for the RTase activity was carried out using $^3$H-dTTP and poly(rA): oligo(dT)12-18 as the substrate.

• 시스템엔지니어링학술지
• /
• 제14권2호
• /
• pp.49-57
• /
• 2018

The main objective of this paper is come up with methodology approach for FPGA-based system in verification and validation lifecycle regarding software reliability using system engineering approach. The steps of both reverse engineering and re-engineering are carried out to implement an FPGA-based of safety critical system in Nuclear Power Plant. The reverse engineering methodology is applied to elicit the requirements of the system as well as gain understanding of the current life cycle and V&V activities of FPGA based-system. The re-engineering method is carried out to get a new methodology approach of software reliability, particularly Software Reliability Growth Model. For measure the software reliability of a given FPGA-based system, the following steps are executed as; requirements definition and measurement, evaluation of candidate reliability model, and the validation of the selected system. As conclusion, a new methodology approach for software reliability measurement using software reliability growth model is developed.

• 대한전자공학회:학술대회논문집
• /
• 대한전자공학회 2000년도 하계종합학술대회 논문집(2)
• /
• pp.40-43
• /
• 2000

The electrons and holes trapped in the tunneling oxide and interface-states generated in the Si/SiO$_2$ interface during program/erase (P/E) operations are known to cause reliability problems which can deteriorate the cell performance and cause the V$_{th}$ window close. This deterioration is caused by the accumulation of electrons and holes trapped in the oxide near the drain and source side after each P/E cycle. we propose three new erase schemes to improve the cell's endurance characteristics: (1)adding a Reverse soft program cycle after the source erase operation, (2)adding a detrapping cycle after the source erase operation, (3)adding a convergence cycle after the source erase operation. (3) is the most effective performance among the three erase schemes have been implemented and shown to significantly reduce the V$_{th}$ window close problem. And we are able to design the reliable periperal circuit of flash memory by using the (3).(3).

• 한국발생생물학회:학술대회논문집
• /
• 한국발생생물학회 2003년도 제3회 국제심포지움 및 학술대회
• /
• pp.70-70
• /
• 2003

The uterus undergoes dynamic changes during the cycle and displays many features typical of developmental process. In order to be prepared for implantation, endometrium undergoes predictable, sequential phases of proliferation and secretory changes. The uterus during estrus cycle synthesize a complex of signaling molecules with specific spatial and temporal modes of expression and which are critical for cell proliferation and differentiation. The purpose of this investigation was to use cDNA microarrays to evaluate the expression of genes of rat uterus in estrus cycle. Animals were sacrificed on proestrus, estrus, metestrus, diestrus. Differential gene expression profiles were revealed(growth-related c-myc reponsive protein RCL, heat shock 47-kDa protein (HSP47), cytochrome c oxidase polypeptide Vlc2 (COX6C2), calreticulin (CALR)). Reverse transcription polymerase chain reaction (RT-PCR) was used to validate the relative expression pattern. Using this approach, we found several genes whose expression in rat uterus was altered with estrus cycle. Our long-term goal is to determine the role of these differentially expressed genes during estrus cycle. This study was supported by through the Biohealth Products Research Center(BPRC), Inje University.

• 한국인터넷방송통신학회논문지
• /
• 제14권4호
• /
• pp.233-241
• /
• 2014

본 논문은 원 그래프를 2-간선 연결 그래프로 단순화하고, 사이클 속성을 적용하여 최소신장트리를 빠르게 얻는 알고리즘을 제안하였다. Borůvka 알고리즘은 정점 (v) 당 최소 가중치 간선 (v) 을 1개씩 선택하는 1-간선 연결 그래프에 대해 사이클 속성을 적용하여 부분신장트리를 얻는다. 추가적으로 절단속성을 적용하여 부분신장트리를 연결하는 최소 가중치 간선을 선택한다. Kruskal 알고리즘은 그래프의 모든 간선을 대상으로 오름차순으로 절단 속성을 적용한다. 역-삭제 알고리즘은 내림차순으로 사이클 속성을 적용한다. Borůvka, Kruskal과 역-삭제 알고리즘은 모든 간선들을 대상으로 하기 때문에 항상 |e| 회 수행된다. 제안된 알고리즘은 첫 번째로, 정점 당 최소 가중치 간선을 2개씩 선택하는 2-간선 연결 그래프를 얻는다. 두 번째로, 2-간선 연결 그래프에 대해 사이클 속성을 적용하여 |e|=|v|-1 일 때 알고리즘을 종료시켰다. 제안된 방법들을 10개의 실제 그래프들에 적용한 결과 모두 최소신장트리를 얻는데 성공하였다. 또한, Borůvka, Kruskal과 역-삭제 알고리즘에 비해 수행 횟수를 60% 단축시켰다.

• KEPCO Journal on Electric Power and Energy
• /
• 제2권2호
• /
• pp.299-305
• /
• 2016

초전도 전력 케이블의 상용화 노력에 따라 점차 장선화 되면서, 단위 냉각 시스템당 냉각용량이 큰 대용량 냉동기의 필요성이 증가하고 있다. 국내에서는 극저온 냉동기에 대한 기술 부족으로 인해 현재 극저온 냉동기는 해외 선진사로부터 고가의 비용으로 수입되고 있다. 초전도 전력 케이블의 상용화를 위해서는 대용량 브레이튼 냉동기의 국내 개발이 시급하다. 대용량 브레이튼 냉동기의 구성은 복열식 열교환기, 압축기, 극저온 터보 팽창기로 구성되어 있으며, 냉동기 효율과 가장 직접적인 연관이 있는 것은 극저온 터보 팽창기이다. 극저온 터보 팽창기는 극저온에서 고속으로 회전하면서 고압의 헬륨 혹은 네온 가스를 팽창시켜 온도를 낮추는 역할을 한다. 본 논문에서는 역브레이튼 냉동 사이클을 설계하고, 이에 적합한 극저온 터보 팽창기를 설계하였다.

• International Journal of Advanced Culture Technology
• /
• 제6권4호
• /
• pp.275-283
• /
• 2018

Cancer show distinct pattern of gene expression when it is compared to normal. This difference results malignant characteristic of cancer. Many cancer drugs are targeting this difference so that it can selectively kill cancer cells. One of the recent demand for personalized treating cancer is retrieving normal tissue from a patient so that the gene expression difference between cancer and normal be assessed. However, in most clinical situation it is hard to retrieve normal tissue from a patient. This is because biopsy of normal tissues may cause damage to the organ function or a risk of infection or side effect what a patient to take. Thus, there is a challenge to estimate normal cell's gene expression where cancers are originated from without taking additional biopsy. In this paper, we propose in-silico based prediction of normal cell's gene expression from gene expression data of a tumor sample. We call this challenge as reverting the cancer into normal. We divided this challenge into two parts. The first part is making a generator that is able to fool a pretrained discriminator. Pretrained discriminator is from the training of public data (9,601 cancers, 7,240 normals) which shows 0.997 of accuracy to discriminate if a given gene expression pattern is cancer or normal. Deceiving this pretrained discriminator means our method is capable of generating very normal-like gene expression data. The second part of the challenge is to address whether generated normal is similar to true reverse form of the input cancer data. We used, cycle-consistent adversarial networks to approach our challenges, since this network is capable of translating one domain to the other while maintaining original domain's feature and at the same time adding the new domain's feature. We evaluated that, if we put cancer data into a cycle-consistent adversarial network, it could retain most of the information from the input (cancer) and at the same time change the data into normal. We also evaluated if this generated gene expression of normal tissue would be the biological reverse form of the gene expression of cancer used as an input.

• 상하수도학회지
• /
• 제35권1호
• /
• pp.39-52
• /
• 2021

The global water shortage is getting more attention by global climate change. And water demand rapidly increases due to industrialization and population growth. Desalination technology is being expected as an alternative water supply method. Desalination technology requires low energy or maintenance costs, making it a competible next generation technology, with examples such as forward osmosis (FO), membrane distillation (MD), capacitive deionization (CDI), and electrodialysis (ED) to compete with reverse osmosis (RO). In order to identify recent research trends in desalination technologies (FO, MD, RO, CDI, and ED) between 2000-2020, a bibliometric analysis was conducted in the current study. The number of published papers in desalination technology have increased in Desalination and Journal of Membrane Science mainly. Moreover, it was found that FO, MD, RO, CDI, and ED technologies have been applied in various research areas including electrochemical, food processing and carbon-based material synthesis. Recent research topics according to the desalination technologies were also identified.

• 대한설비공학회:학술대회논문집
• /
• 대한설비공학회 2006년도 동계학술발표대회 논문집
• /
• pp.88-88
• /
• 2006

• 대한설비공학회:학술대회논문집
• /
• 대한설비공학회 2007년도 하계학술발표대회 논문집
• /
• pp.24-24
• /
• 2007