• BMB Reports
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• v.49 no.1
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• pp.3-10
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• 2016

microRNAs (miRNAs) are key regulators of cell state transition and retention during stem cell proliferation and differentiation by post-transcriptionally downregulating hundreds of conserved target genes via seed-pairing in their 3' untranslated region. In embryonic and adult stem cells, dozens of miRNAs that elaborately control stem cell processes by modulating the transcriptomic context therein have been identified. Some miRNAs accelerate the change of cell state into progenitor cell lineages—such as myoblast, myeloid or lymphoid progenitors, and neuro precursor stem cells—and other miRNAs decelerate the change but induce proliferative activity, resulting in cell state retention. This cell state choice can be controlled by endogenously or exogenously changing miRNA levels or by including or excluding target sites. This control of miRNA-mediated gene regulation could improve our understanding of stem cell biology and facilitate their development as therapeutic tools. [BMB Reports 2016; 49(1): 3-10]

• The Korean Journal of Ecology
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• v.19 no.4
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• pp.321-328
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• 1996

The profile and concentration of monoterpene metabolites in the leaf and stem of Artemisia princeps var. orientalis were quantified, and seasonal variation in monoterpenes of Artemisia plant was investigated. Samples were taken from five sites at the campus of Kyungnam University during maturing season. Monoterpenes in leaf and stem were analyzed using gas chromatography-mass spectrometry (GC-MS). The major constituents of A. princeps var. orientalis in both the leaf and stem were 21 monoterpenes.$ {\alpha}-pinene,\;{\beta}-pinene,\;{\beta}-myrcene$, dl-limonene, naphthalene and unknown monoterpenes with 5.49 and 16.27 of retenstion time were present in high concentrations of compounds identified on the leaf and stem of A. princeps var. orientalis. The cmounts of total monoterpenes of leaf were from two to five times higher than stem and rapidly decreased with the time, while that of stem was constnat except early spring. Most of the high percentage of monoterpenes in the leaf were those with later retention time. These results indicated that monoterpenes yields are considered to be more variable than monoterpene composition in responding to the time in both the leaf and stem.

• Korean journal of food and cookery science
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• v.18 no.4
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• pp.461-469
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• 2002

In order to develop a better food preparation method in terms of vitamin retention in food service establishments, the retention rate of riboflavin in two kinds of Korean dish (stir-fried sausage with vegetables, stir-fried garlic young stems) were examined during various cooking stages, holding temperature and duration time. Riboflavin content of the sample was analyzed using by using a HPLC with a fluorescent detector at various holding durations and temperatures. Also the changes in the pH and water contents of the samples were measured during holding at various temperatures to find any relation among the pH, water contents and riboflavin content. In the results, there were significant differences in riboflavin contents at various cooking processes such as washing, slicing, blanching, soaking, etc. Also, the retention rates of riboflavin at various holding methods and temperature were significantly different from one another. Especially 50% reduction of riboflavin content occurred during blanching of young garlic stem and only 38% of riboflavin was left after slicing carrots for stir-frying of sausage.

• The Korean Journal of Ecology
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• v.20 no.6
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• pp.397-403
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• 1997

The monoterpenes and sesquiterpenes are analysed in the leaf and stem of Chrysanthemum boreale using gas chromaltography-mass spectrometry (GC-MS). The total amount of sesquiterpenes are always higher than monoterpenes in both leaf (2.0-3.4 times) and stem (1.6-8.3 times). The mono- and sesquiterpenes yields of the leaf are higher than the stem. There was no significant difference among the leaf developmental stages, while those of stem were varied. Seventeen monoterpenes and 9 sesquiterpenes compound in this plants comprised more than 5% of the mean total monoterpenes and the total sesquiterpenes in each dates. Among leaf monoterpenes, the concentration of (+)-Limonene and unknown compound no. 13 (Retention time, R.T.=17.28) varied significantly during leaf growing season, and the concentrafion of unknown compound no. 7 (R.T.=35.04) and no. 9(R.T.=35.71) varied in the leaf sesquiterpenes. Similarly the results from the leaf, the concentration of five monoterpenes in stem also varied significantly during maturing period, and much varied in seven compounds of stem sesquiterpene. The major sesquiterpenes of leaf and stem were ${\alpha}-Humulene$ and compound no. 2(R.T.=26.19).

• Journal of Engineering Education Research
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• v.23 no.4
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• pp.37-51
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• 2020

This study examined the differential effects of High-Impact Practices(HIPs) on the career aspiration of STEM college students by gender. Through the theoretical lens of Social Cognitive Career Theory(SCCT), a two-level model analysis was conducted. A sample of 2,101 third- and fourth-year undergraduate students majoring in STEM at 38 universities, which had been collected from the National Survey on College Student Experiences and Learning Outcomes funded by the Korea Research Foundation, was used. This study found that the three HIP domains(learning with peers, faculty support, content relevancy) had different influences depending on gender. These findings suggest that HIPs can benefit the development of female students' career aspiration and have gender-differential effects on students in STEM majors. Based on those findings, this study also deduced implications about the roles of faculty members and higher-education institutions that might foster the retention of women in STEM.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.6
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• pp.803-811
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• 2009

The chemical composition and water extractable dry matter (DM) of foliages from Erythrina (Erythrina variegata), Fig (Ficus racemosa), Jackfruit (Artocarpus heterophyllus), Jujube (Ziziphus jujuba Mill), Kapok (Ceiba pentandra) and Mango (Mangifera indica) and the feed intake, digestibility and N retention when feeding these foliages were studied in two experiments. In Experiment 1, 12 male goats, 3.5 months old and weighing 14.6 kg, were randomly allocated to a diet consisting of one of the foliages in a change-over design with three periods. The foliages were offered ad libitum at the level of 130% of the average daily feed intake. The Erythrina foliage had a low content of DM and condensed tannins (CT) and a high concentration of crude protein (CP) in leaves plus petioles (193 g/kg DM) and stem, while the Mango foliage had a low CP (69 g/kg DM) and high DM content. The other foliages were intermediate. High content of CT was found in the leaves plus petioles of Jackfruit foliage and in the stem of Fig and Mango foliage. There was a difference in feed intake, nutrient intake, apparent digestibility and N retention between the foliages, with Erythrina, Jackfruit and Kapok foliage being significantly higher in these parameters than Fig, Jujube and Mango foliage. The water extractable DM could be used to estimate N retention, but not DM digestibility in this study. In Experiment 2, 4 male goats weighing 13.4 kg and 6 months old were allocated to a 4${\times}$4 Latin square design. The treatments were: water spinach ad libitum and Fig, Jujube or Mango foliage ad libitum +0.5% of BW as water spinach DM. Feed intake, apparent digestibility and N retention were not significantly different among the foliage diets, but higher than for water spinach alone (p<0.05). Supplementation with water spinach to a diet consisting of low quality foliages such as Fig, Jujube and Mango, increased DM and CP intake, apparent digestibility and N retention, compared to feeding these foliages as sole feeds.

• Asian-Australasian Journal of Animal Sciences
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• v.10 no.1
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• pp.106-113
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• 1997

A field experiment was carried out on a dryland farming area of southern Bali for 92 weeks, to study the growth and fodder yield of 16 provenances of Gliricidia sepium in guardrow system. The experimental design was completely randomized blocks of 16 treatments (Gliricidia sepium provenances) replicated 3 times, with 6 plants per provenance. Six provenances were from Mexico (M), four from Guatemala (G), and one each from Colombia (C), indonesia (I), Nicaragua (N), Panama (P), Costa Rica (R) and Venezuela (V). After 40 weeks establishment the gliricidia were lopped 4 times a year at 150 cm height, at 2 months intervals during the 4 month wet season and 4 month intervals during the 8 month dry season. Stem elongation varied from 21 to 81 cm, leaf retention from 39 to 240%, branch number from 12 to 35, fodder yield from 1,090 to 3,153 g DW/plant. and wood yield from 743 to 2,750 g DW/plant. Pontezuelo provenance of Colombia (C24), Belen provenance of Nicaragua (N14) and Retalhuleu provenance of Guatemala (G14) were ranked first, second and third, respectively, for stem elongation, leaf retention, fodder and wood yields, during the wet and dry seasons.

• Food Science of Animal Resources
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• v.41 no.5
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• pp.749-762
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• 2021

Colostrum, which contains various immune and growth factors, aids wound healing by promoting keratinocyte proliferation. Aquaporins (AQPs) are small, hydrophobic membrane proteins that regulate cellular water retention. However, few studies have examined the effect of processed colostrum whey on AQP-3 expression in human skin cells. Here, we investigated the effect of milk, colostrum, fermented milk, and fermented colostrum whey on AQP-3 expression in keratinocyte HaCaT cells. Concentrations of 100-400 ㎍/mL of fermented colostrum whey were found to induce HaCaT cell proliferation. AQP-3 was found to be expressed exclusively in HaCaT cells. AQP-3 expression was significantly increased in 100 ㎍/mL fermented colostrum whey-treated cells compared with that in controls. Moreover, fermented colostrum increased p38 mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinase (JNK) phosphorylation, but not ERK1/2 phosphorylation. Thus, our results suggest that fermented colostrum whey increased AQP-3 expression in, and the proliferation of, keratinocytes via JNK and p38 MAPK activation.

• Asian-Australasian Journal of Animal Sciences
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• v.25 no.4
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• pp.471-478
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• 2012

The role of moisture absorptive capacity of pre-silage material and its relationship with silage effluent in high moisture by-product feedstuffs (HMBF) is assessed. The term water retention capacity which is sometimes used in explaining the rate of effluent control in ensilage may be inadequate, since it accounts exclusively for the capacity of an absorbent incorporated into a pre-silage material prior to ensiling, without consideration to how much the pre-silage material can release. A new terminology, 'potential water retention capacity' (PWRC), which attempts to address this shortcoming, is proposed. Data were pooled from a series of experiments conducted separately over a period of five years using laboratory silos with four categories of agro by-products (n = 27) with differing moisture contents (highest 96.9%, lowest 78.1% in fresh matter, respectively), and their silages (n = 81). These were from a vegetable source (Daikon, Raphanus sativus), a root tuber source (potato pulp), a fruit source (apple pomace) and a cereal source (brewer's grain), respectively. The pre-silage materials were adjusted with dry in-silo absorbents consisting wheat straw, wheat or rice bran, beet pulp and bean stalks. The pooled mean for the moisture contents of all pre-silage materials was 78.3% (${\pm}10.3$). Silage effluent decreased (p<0.01), with increase in PWRC of pre-silage material. The theoretical moisture content and PWRC of pre-silage material necessary to stem effluent flow completely in HMBF silage was 69.1% and 82.9 g/100 g in fresh matter, respectively. The high correlation (r = 0.76) between PWRC of ensiled material and silage effluent indicated that the latter is an important factor in silage-effluent relationship.

• Korean Journal of Food Science and Technology
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• v.37 no.3
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• pp.360-363
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• 2005

To detect falcarinol in Acanthopanax senticosus, its stem and bark were extracted with methanol fractionated with petroleum ether and diethyl ether, and separated by silica gel column chromatography, Resulting six crude samples were compared with standard falcarinol through TLC on silica gel plates. Fraction 6 showed $R_{f}$ value of 0.46 similar to that of standard falcarinol. Through analytical reverse phase HPLC/PDA, UV spectra of standard falcarinol and traction 6 recorded between 200 and 340 nm showed identical peaks and UV spectra patterns. GC/MS revealed standard falcarinol and fraction 6 have equal retention times of 7.4 and 8.5 min before and after TMS-derivatization, respectively. Standard falcarinol and fraction 6 showed more similar spectrum patterns after TMS-derivatization than before. These results confirm presence of falcarinol in A. senticosus.