• Biomedical Science Letters
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• v.11 no.2
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• pp.115-119
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• 2005

A total of 28 Salmonella enterica serovar Typhimurium isolated from diseased pigs and swine carcasses between 2001 and 2003 were characterized by the antimicrobial resistance profiles, PCR for detection of S. Typhimurium DT104 and pulsed-field gel electrophoresis (PFGE) with the restriction enzyme XbaI. All but one isolate presented multidrug resistance (MDR) to more than two antibiotics tested. A total of 11 resistance profiles were observed, and two phenotypes, ST and ASSuTG, were the most common among them. Two isolates were found to be S. Typhimurium DT104 isolates by PCR, and their resistance profile did not show the DT104 typical resistance type ACSSuT, but ACSSuTGK instead. PFGE identified 11 banding patterns in dendrogram, and three main clusters (designated A to C) were represented. Interestingly, sixteen of 19S. Typhimurium isolates belonging to cluster B showed an identical band pattern.

• Proceedings of the KIEE Conference
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• 2002.11c
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• pp.585-588
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• 2002

Grid provides integral ing system that enables to use distributed computing resource and services as adapts traditional infrastructures to overcome the distributed computing environments. But, computing today is moving away from a restriction of the desktop, becoming diffused into our surrounding and onto our personal digital devices. In such mobile computing environments, users expects to access resource and services at any time from anywhere in such Mobile-Grid computing. This expectation results security issues, since the computing environments is expanded. This paper describes the security challenges in Mobile-Grid computing, explaining why traditional security mechanism fail to meet the demands of these environments. This paper describes policy driven security mechanism enabled entity to use service and data in trust Mobile-Grid environments and a set of security service module that need to be realized in the Mobile-Grid security architecture presents a set of use pattern that show hew these modules can be used for billing service in a secure Mobile-Grid environments.

• Communications for Statistical Applications and Methods
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• v.25 no.5
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• pp.559-568
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• 2018

Gene classification can involve complex order-restricted inference. Examining gene expression pattern across groups with order-restriction makes standard statistical inference ineffective and thus, requires different methods. For this problem, Roy's union-intersection principle has some merit. The M-estimator adjusting for outlier arrays in a microarray study produces a robust test statistic with distribution-insensitive clustering of genes. The M-estimator in conjunction with a union-intersection principle provides a nonstandard robust procedure. By exact permutation distribution theory, a conditionally distribution-free test based on the proposed test statistic generates corresponding p-values in a small sample size setup. We apply a false discovery rate (FDR) as a multiple testing procedure to p-values in simulated data and real microarray data. FDR procedure for proposed test statistics controls the FDR at all levels of ${\alpha}$ and ${\pi}_0$ (the proportion of true null); however, the FDR procedure for test statistics based upon normal theory (ANOVA) fails to control FDR.

• Journal of ILASS-Korea
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• v.4 no.4
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• pp.17-23
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• 1999

Recently, many researchers have been studied a D.I. diesel engine because of the exhaust gas restriction and fuel consumption performance. It is well known that the fuel injection characteristics are the key factors on the diesel combustion and exhaust emission. In this study, the fuel injection characteristics of 5-hole injector and the combustion characteristics are investigated with the amount of fuel by means of the visualization method and visualization D.I. diesel engine system. As the results of the experiments, the spray pattern of the fuel injection and the diffusion flame of a D.I. diesel engine are clarified. In addition, combustion phenomena with operation conditions such as engine speed and engine load are made clear.

• Journal of Environmental Science International
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• v.8 no.1
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• pp.135-144
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• 1999

Air quality modeling about coastal urban region such as Pusan shoud be consider shipping source emmited from ships anchoraging and running. It has been proved at our previous studies that the ratios of air pollutants emission amount in coastal area to inland are 12.2% for NO$_2$ and 11.7% for $SO_2$ and the air qualify of coastal urban area consierably counts on ships. Also the dispersion pattern of the all pollutants followed local circulation system in this region. Therefore this study has been developed air quality model which can describe the formation, transport, transformation and deposition processes of air pollutants considering shipping source. Currently, restriction for emission amount of ships does not exist, so our study will be useful to set the omission standard and for devising air quality policy in coastal urban region.

• Archives of Pharmacal Research
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• v.21 no.4
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• pp.385-390
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• 1998

Adozelesin and carzelesin are synthetic analogues of the extremely potent antitumor antibiotic CC-1065, which alkylates N3 of adenine in a consensus sequence $5^1$-(A/T)(A/T)$A^*$ ($A^*$ is the site of alkylation). We have investigated the DNA sequence selectivity of adozelesin and carzelesin by thermally ind ced DNA strand cleavage assay using radiolabeled restriction DNA fragments. An analysis of alkylation patterns shows that the consensus sequences for carzelesin and adozelesin have been found to be $5^1$-(A/T)(A/T)$A^*$ and $5^1$-(A/F)(G/C)(A/T)$A^*$. A new consensus sequence, $5^1$-(A/T)(A/T)$CA^*$, has been observed to display an additional alkylation site for adozelesin but not for carzelesin. These results indicate that the pattern of sequence selectivity induced by carzelesin is similar but not identical to those induced by adozelosin.

• Bulletin of the Korean Chemical Society
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• v.20 no.5
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• pp.539-542
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• 1999

The diastereoselectivity in the photocyclization of α-(o-ethylphenyl)acetophenone(1) to 1-methyl-2-phenyl-2-indanol was studied in zeolites X and Y, and compared with that in isotropic solvents. The yields of E-diastereomer at the irradiation of 1 in protic solvents were higher than those in aprotic solvents. The ratios of E-diastereomer to Z-diastereomer in zeolites X and Y were much higher than those in isotropic solvents. The E/Z ratios at the irradiation of 1 in zeolites X were also found to be dependent on the cations present. As the cation size increased from Li+ to Cs+, the ratio decreased. However, any pattern in the E/Z ratios was not found in zeolites Y. The diastereoselectivity observed in the zeolites was interpreted by the conformational restriction imposed by the cavity size. An efficient oxidation of 1 in zeolites RbX and CsX was also observed.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.32 no.5
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• pp.357-360
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• 2019

We demonstrate an alignment technology using an imprinting process on an inorganic NiOx film. The aligned nanopattern was fabricated on a silicon wafer by laser interference lithography. The aligned nano pattern was then imprinted onto the sol-gel driven NiOx film using an imprinting process at an annealing temperature of $150^{\circ}C$. After the imprinting process, parallel grooves had been formed on the NiOx film. Atomic force microscopy and water contact angle measurements were performed to confirm the parallel groove on the NiOx film. The grooves caused liquid crystal alignment through geometric restriction, similar to grooves formed by the rubbing process on polyimide. The liquid crystal cell exhibited a pretilt angle of $0.2^{\circ}$, which demonstrated homogeneous alignment.

• The Plant Pathology Journal
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• v.22 no.1
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• pp.36-45
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• 2006

Twenty isolates of Didymella bryoniae were isolated from infected cucurbit plants in various growing areas of southern Korea in 2001 and 2002. Random Amplified Polymorphic DNA (RAPD) group [RG] I of D. bryoniae was more virulent than RG IV to watermelon. Virulence of the RG I isolate was strong to moderate to cucumber, whereas that of the RG IV varied from strong, moderate to weak. Two hundred seventy-three amplified fragments were produced with 40 primers, and were analyzed by a cluster analysis using UPGMA method with an arithmetic average program of NTSYSPC. At the distance level of 0.7, two major genomic DNA RAPD groups were differentiated among 20 isolates. The RG I included 7 isolates from watermelon and one isolate from melon, whereas the RG IV included 12 isolates from squash, cucumber, watermelon and melon. Amplification of internal transcribed spacer (ITS) region and small subunit rRNA region from the 20 isolates yielded respectively a single fragment. Restriction pattern with 12 restriction enzymes was identical for all isolates tested, suggesting that variation in the ITS and small subunit within the D. bryoniae were low. Amplification of the genomic DNAs of the tested isolates with the sequence characterized amplified regions (SCAR) primer RG IF-RG IR specific for RG I group resulted in a single band of 650bp fragment for 8 isolates out of the 20 isolates. Therefore, these 8 isolates could be assigned into RG I. The same experiments done with RG IIF-RG IIR resulted in no amplified PCR product for the 20 isolates tested. An about 1.4 kb-fragment amplified from the RG IV isolates was specifically hybridized with PCR fragments amplified from genomic DNAs of the RG IV isolates only, suggesting that this PCR product could be used for discriminating the RG IV isolates from the RG I isolates as well other fungal species.

• Korean Journal of Animal Reproduction
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• v.20 no.2
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• pp.89-99
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• 1996

The purpose of this study was to develop the diagnosis techniques for sex determination of rabbit embryos at preimplantation stage. To detect male specific sequences using polymerase chain reaction, two genes functional on sex determination including SRY and ZFX/Y genes were targeted using multiple oligonucleotide primer sets. Three of them for conserved SRY gene were used for appropriate amplification pattern, and then only one primer set #3 proved to be most efficient, showing male-specific strong signal ofamplified sequences. Using this male specific bandsfrom human, cattle, pig and mouse, the gender of rabbit was determined. As an another system for sex determination system, amplified 910bp fragment from ZFX/Y was digested with several restriction endonuclease and showed gender specific restriction fragments only by Hinf I. Using two different system for sex identification of rabbit in this study, blind tests for 17 samples was conducted and showed identical results from two different methods. And then, amplification limit of PCR reaction for template DNA was estimated using various amounts of DNA for both SRY and ZFX/Y systems, resulted as 20pg and 800pg, respectively. With this results, test for gender identification of rabbit embryos were performed using SRY derived amplification system. From total 22 embryos selected for its developmental state 18 were identified as male embryos, showing significant difference from expected sex ratio 1:1. This biased sex ratio was interpreted as to have been caused by the fact, reported by the fact, reported by several researchers, that male embryos develop more rapidly and are more resistant against the in vitro manipulation than female embryos.