• Korean Journal of Microbiology
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• v.26 no.2
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• pp.73-81
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• 1988

$\alpha$-Amylase (EC.3.2.1.1) of Neurospora crassa (ATCC9279) was cloned in E. coli HB101 using shotgun method, and the enzymes isolated from both N. crassa and E. coli were compared. Chromosomal DNA isolated from the spores of N. crassa was partially digested with PstI restriction endonuclease and rejoined to pBR322 which had been digested with the same enzyme. The resulting recombinant DNA were introduced into E. coli HB101 which had competancy by treating with $CaCl_{2}$. As the result, about 8000 colonies which showed tetracycline resistance were selected and two of the colonies which had 13.5Kb recombinant plasmid exhibit starch degrading activity on starch-containing plate when treated with D-cycloserine. $\alpha$-Amylases from both N.crassa and E. coli were isolated by using ammonium sulfate precipitation, DEAE-cellulose ion exchange column chromatography and Bio-Gel P150 gel foltration column. As the result, about 81.3 fold and 5.6 fold purifications in specific activities were obtained respectively, and specific activities of the gel filtrates were 6.1u/mg and 85u/mg respectively. The properties of both enzymes were compared and they showed quite the similar patterns in optimal temperature, optimal pH and had same molecular weight about 100,000 daltons on gel filtration method. Optimal temperatures for both enzymes were $70^{\circ}C$ and optimal pH were about 6 and 10.

• The Journal of Fisheries Business Administration
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• v.44 no.1
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• pp.15-24
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• 2013

Recently the Korean court accepts two typies of fisheries damages caused by oil pollution.. One is the direct loss of fisheries production originated from pollution of oil spillover. The other is the indirect loss of fisheries production originated from governmental measure of restricting fishing activities because of safety of marine product of oil spillover areas. The paper tries to suggest the appropriated measure of oil pollution damages of hanging and floating netcage aquaculture fisheries using the court judgement for responsibility restriction on Herbei Spirit Case. The paper tries to compare the damge estimation method of floating netcage aquaculture fisheries with that of natural aquaculture fisheries using conventional theory of the population biology of living resources characterized with age distribution.

• The Journal of Pediatrics of Korean Medicine
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• v.18 no.1
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• pp.131-138
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• 2004

Background: Epilepsy is a disorder of the central nervous system characterized by loss of consciousness and convulsions. Epilepsy, a typical chronic disease, may cause high level of psychosocial difficulties for all family members, including stigmatization, stress, martial problems, poor self esteem and restriction of social activities. Objective & Method: To demonstrate the therapeutic effect of herbal medicine (Kamigoakhyangjeonggisan) on patients with epilepsy. We treated two cases of epileptic children, who had frequent seizures. Results: Two children improved in the frequency of the seizure, the combined disabilities. Conclusion: We report that we had good effects of herbal medicine treatment on two cases of epileptic children.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.126-126
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• 2018

Many of researches have revealed that human intestinal microbiota is related to health. Several diseases like obesity, diabetes, and hypertension are affected by the microbiota directly and indirectly. So, interventions with food and drug have been tried to change a composition of the microbiota to better condition. However, few natural medicines have elucidated to date. To understand an influence on microbiota by plant materials including Glycyrrhizae Radix, the extract of medicines were administered to mice and the feces were collected before and after the administration. The feces were analyzed by terminal restriction fragment length polymorphism (T-RFLP). The changes in composition of mice gut microbiota were detected and analyzed. The data could be utilized to further study about biological activities of the plant medicines.

• Korean Journal of Microbiology
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• v.27 no.3
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• pp.188-193
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• 1989

Gene for lactose catabolism in Lactobacillus casei SW-M1 was encoded by a 60Kb metabolic plasmid. A derivative of only 10kb, pPlac 15 of recombinant plasmid, was constructed by introducing into pBR322 and was cloned into E. coli using restriction endonuclease Pst I. A 10kb insery DNA in plasmid pBR322 was identified as a gene encoded phospho-$\beta$-galactosidase by the determination of enzyme activity. Phospho-$\beta$-galactosidase was apparently expressed in E. coli. The enzyme activities of cell-free extract from transformant E. coli HB101 carrying pPLac 15 DNA were not different from that of L. casei as a donor strain on the basis of enzyme properites. However, specific activity of phospho-$\beta$-galactosidase in the cloned strain with Lac $Y^{-}$ phenotype of E. coli HB101 was lower than that in L. casei strain.

• Journal of Ginseng Research
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• v.21 no.2
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• pp.78-84
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• 1997

As a part of our ongoing effort to develop new antineoplastic immunostimulator from natural sources, bioassay-directed fractionationn of polysaccharides from Korean red ginseng was carried out by observing the proliferation of marine spleen cells and the generation of lymphoklne activated killer (LAK) cells. The acidic polysaccharide fractions proliferated spleen cells and generated LAK cells in proportion to their acidity in vitro. The LAK cell which was induced by ginseng showed tumoricidal activity against both NK celt sensitive and insensitive tumor target cells without major histocompatibility (MHC) restriction. Adherent macrophages and CD4+helper T cells were involved in the generation of the LAK cells. The acidic polysaccharide from Korean red ginseng synerglzed with recombinant IL-2 (rIff-2) at lower than 3 U/ml. The optimal doses of the acidic polysaccharide from Korean red ginseng for the proliferation of spleen cells and for the generation of LAK cells were 1 mg/ml and 100 $\mu\textrm{g}$/ml, respectively; this means that the mechanisms for the both activities may be different from each other.

• Journal of Institute of Control, Robotics and Systems
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• v.14 no.1
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• pp.62-69
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• 2008

A specialized anthropomorphic robot hand which can be attached to the biped humanoid robot MAHRU-R in KIST, has been developed. This built-in type hand consists of three fingers and a thumb with total four DOF(Degrees of Freedom) where the finger mechanism is well designed for grasping typical objects stably in human's daily activities such as sphere and cylinder shaped objects. The restriction of possible motions and the limitation of grasping objects arising from the reduction of DOF can be overcome by reflecting a typical human finger's motion profile to the design procedure. As a result, the developed hand can imitate not only human hand's shape but also its motion in a compact and efficient manner. Also this novel robot hand can perform various human hand gestures naturally and grasp normal objects with both power and precision grasping capability.

• Journal of the Korean Home Economics Association
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• v.33 no.3
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• pp.165-177
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• 1995

The home environments, daily activities, caregiving behaviors of mothers, infant's verbal behaviors, attachment to mothers of home-reared infants from low SES families were observed. 30 two-year-old home-reared infants and their mothers were observed at home by the time sampling method. The HOME scale, the behavioral checking lists for caregiving behaviors and for the infants' verbal behaviors, and the Attachment Q-set were used for the observation. the total HOME scores were in the middle range and the scores of "Provision of apropriate play materials" was relatively high, but "Avoidance of restriction and physical punishment" was low. The most frequent caregiving behaviors shown by mothers were " Facilitating verbal development" and "Positive social-emotional inputs". The most frequent verbal behaviors shown by infants were " Information exchange" and " Language interpretation" . The infants' attachment security scores of their mothers tended to be low.

• Journal of Electrical Engineering and Technology
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• v.12 no.1
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• pp.396-401
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• 2017

In this paper, a device to analyze gait pattern was developed by using a 2-axis acceleration sensor attached to the foot. The 1st low-pass filter was adapted to limit the frequency band up to 5 Hz. An algorithm to detect the peak value exceeding the threshold voltage of an X-axis acceleration sensor and a Z-axis acceleration sensor was developed and normal and abnormal walking patterns were thus differentiated. Also, MCU and Bluetooth were combined to transfer the data to other MCUs to display on an LCD; the size of the device could then be reduced. The new algorithm and the device allowed the individual walking patterns to be easily measured at a low cost and with less restriction on activities compared to conventional multiple pressure sensors or motion camera system.

• Korean Journal of Microbiology
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• v.24 no.2
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• pp.86-90
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• 1986

The DNA methylated by Hpa II methylase was not cleaved by Sma, I, Ava I and Nae I endonucleases. This experimental data could be interpreted as strong evidences that Sma I, Ava I and Nae I methylases which yet to be isolated would methylate on the inmost cytosine nucleotide within their hexameric recognition sequences. The facts that Sma I, Ava I and Nae I endonucleases can not cleave the DNA methylated by Hpa II methylase are the valuable informations for protecting DNAs upon cleavage reactions by Sma I, Ava I and NAe I endonucleases especially for cDNA insertion experiments into vector DNAs using Sma I, Ava I and Nae I oligonucleotide linkers. In the case of Xma I endonuclease, partially cleaved DNA fragments were observed although the reaction rate was greatly decreased. This result implies that the methylation site of Xma I methylase which yet to be isolated would not be the same as that of Hpa II methylase in Xma I sequence.