• 한국가금학회지
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• 제36권4호
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• pp.329-335
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• 2009

본 연구에서는, 3개의 다른 색(white, blue 및 red/ 23L-1D)의 조명이 육계 암수별 행동 및 생산성에 미치는 영향에 대해서 조사하였다. 시험계는 초생추 육계를 6개 pan(pan당 암컷 16수, 수컷 16수)으로 나누어서, 3가지 색의 조명에서 실험이 실시되었다. 실험계는 3일간의 조명에 대한 적응 기간을 거친 후, 동일한 사양 조건에서, 암수 각각 3수씩, pan당 6마리의 focal birds를 중심으로 pecking, resting, standing 및 walking 행동이, 1일 120분간(pan당 20분/일), 주 5일간, 직접관찰법으로 5주간 시행되었다. 뿐만 아니라 증체량과 사료 섭취량 등은 주별로 계산되었고, 시험계 19일령과 33일령의 2회에 걸쳐서 TI-반응과, 33일령의 1회에 걸쳐서 gait score, foot pad score 및 horn burn score가 조사되었다. 조명의 색은 증체량, 사료 섭취량에는 유의한 영향을 미치지 않았다(P>0.05). 그러나 사료 효율에는 유의한 영향을 미쳐서(P<0.05), blue 조명의 경우가 white 및 red 조명의 경우보다 높았다(P<0.05). Resting 행동 빈도는 전체의 행동 빈도에서 주요한 행동으로 나타났으며, 이는 암수간 또는 조명의 색깔에 따라서 영향을 받지 않았다. 성장 초기에는 red 조명에서 resting 행동 빈도가 낮게 나타나는 경향이 있었다. 뿐만 아니라 standing 행동 빈도는 4~18일령에, walking 행동 빈도는 전 시험 기간 동안 red 조명에서 높게 나타나는 경향이 있었다. 본 연구 결과로부터 육계 사육장내 조명의 색은 생산성, 행동 빈도 및 복지에 영향을 미칠 가능성이 생각되었다.

• 한국가금학회지
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• 제37권1호
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• pp.43-49
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• 2010

본 연구에서는 육계의 성비가 행동 및 복지지표에 미치는 영향에 대해서 조사하였다. 암수의 성비를 0:100 (T1), 25:75(T2), 50:50 (T3), 75:25 (T4) 및 100:0 (T5)의 5 처리를 하여서 4주간 조사하였다. 처리구에서 전체 행동 중 resting 행동 빈도가 74.4% 이상으로 가장 많이 차지하였다. 그 외 pecking 행동이 5.39∼23.07%, standing 행동이 1.78∼24.75%, moving 행동이 0.59∼11.46%로 나타났다. Pecking, standing resting 및 moving 행동 빈도는 성비 및 암수간에 유의한 차이는 인정되지 않았다(P>0.05). 그러나 pecking, resting 및 moving 행동 빈도는 일령의 변화에 따라서 유의한 차이가 인정되었다(P<0.05). Gait, foot pad 및 hock burn score는 성비의 차이에 따른 영향을 받지 않았지만, 모든 처리구에서 수컷보다 암컷에서 감소하는 경향이 나타났다. 21일령과 35일령에서의 발톱의 길이는 성비에 따라서, 또는 암수간에 차이는 인정되지 않았다. TI-reaction은 21령이 35일령보다, 암컷이 수컷보다 낮아지는 경향이 인정되었다. 본 연구에서 T4 처리구의 hock burn score, 발톱의 길이 및 TI-reaction이 다른 처리구보다 낮아지는 경향이 인정되었다. 결론적으로 육계를 암수 혼합 사육할 때, 암수의 성비는 육계의 복지지표에 영향을 미칠 가능성이 생각된다.

• 식물병연구
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• 제6권1호
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• pp.27-33
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• 2000

심하게 이병된 배추포장내 무사마귀병균의 수직분포를 보면 토양의 심도가 깊어질수록 무사마귀병균의 밀도는 급격히 감소하였으며 무사마귀병균의 97%가 지하 5cm 이내의 표토에 분포하였고 지하 40cm 토양에서도 소량 검출되었다. 수평분포를 보면 무사마귀병균은 배추포기중심에 모여 있다기보다는 전 포장에 골고루 분포하고 있어서 한곳에 몰려 분포하는 현상은 발견되지 않았다. 평창의 23개 배추지역의 무사마귀병균의 밀도는 1$\times$$10^4$포자/g 토양 이하의 밀도가 낮은 곳에서부터 $10^{6}$ 토양이상의 밀도가 높은 포장까지 폭넓게 분포하고 있었다. 작물을 재배하기 않은 처녀지 토양은 밀도가 $10^4$토양 이하로 극히 낮거나 전혀 검출되지 않았으며 심하게 이병된 배추밭의 밀도는 $10^{5}$/g 토양이상으로 대단히 높았다. 건전 배추밭과 배추 이외의 타작물을 재배하고 있는 포장내 밀도는 이보다 상당히 낮아서 심고 있는 작물에 따라서 토양내 무사마귀병균 밀도에 큰 차이가 있었다 작부체계별 밀도분포를 보면 다소 예외는 있으나 배추, 무 등의 기주작물을 연작한 곳일수록 밀도가 높게 나타났으며, 약초, 옥수수, 메밀, 기타 채소 등 비기주작물을 재배한 곳일수록 무사마귀병균의 밀도가 낮은 곳이 많았다. 배추, 무 등의 기주작물을 연작하면 토양내 무사마귀병균의 밀도는 점차 상승하는 경향이었고 반면에 당귀, 메밀 등 비기주작물로 윤작한 포장의 밀도는 급격히 감소하는 경향이어서 무사마귀병균의 토양내 생존은 작부체계에 의해 크게 영향을 받고 있는 것으로 나타났다. 본 연구에서 사용한 개량형광염색방법은 기존의 방법에 비해 토양내 휴면포자의 식별이 보다 용이해졌을 뿐만 아니라 그 검출효율도 증진되었고 조작이 간편하여 큰 숙련없이 밀도를 측정할 수 있어 향후 토양내 무사마귀병균의 동태를 연구하는데 유용하게 이용될 수 있을 것으로 생각된다.다.

• Journal of Microbiology and Biotechnology
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• 제27권8호
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• pp.1428-1440
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• 2017

Phomopsis sp. XP-8 (an endophytic fungus) was previously found to produce pinoresinol diglucoside (PDG), a major antihypertensive compound of Tu-Chung (the bark of Eucommia ulmoides Oliv.), which is widely used in Chinese traditional medicines. In the present study, two bioconversion systems were developed for the production of PDG in Tris-HCl buffer containing glucose and Phomopsis sp. XP-8 cells (both resting and freeze-dried). When other factors remained unchanged, the bioconversion time, glucose concentration, cell ages, cell dosage, pH, temperature, and stirring speed influenced PDG production in a similar and decreasing manner after an initial increase with increasing levels for each factor. Considering the simultaneous change of various factors, the optimal conditions for PDG production were established as 70 g/l cells (8-day-old), 14 g/l glucose, $28^{\circ}C$, pH 7.5, and 180 rpm for systems employing resting cells, and 3.87 g/l cells, 14.67 g/l glucose, $28^{\circ}C$, pH 7.5, and 180 rpm for systems employing freeze-dried cells. The systems employing freeze-dried cells showed lower peak PDG production ($110.28{\mu}g/l$), but at a much shorter time (12.65 h) compared with resting cells (23.62 mg/l, 91.5 h). The specific PDG production levels were 1.92 and $24{\mu}g$ per gram cells per gram glucose for freeze-dried cells and resting cells, respectively. Both systems indicated a new and potentially efficient way to produce PDG independent of microbial cell growth.

• 대한세포병리학회지
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• 제6권2호
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• pp.106-115
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• 1995

Reactive humsn mesothelial cells were examined by immunocytochemical stain with intermediate filaments (cytokeratin [CK1, CK7, CK8, CK18, CD19), vimentin, desmin, actin), epithelial membrane antigen, carcinoembryonic antigen (CEA), MHC class II antigen (HLA-DR), LeuM-1 (CD15), $\alpha1-antitrypsin$(ACT), $\alpha1-antichymotrypsin$ (ACHT), CD68(KP-1) and FcyRIII(CD16). The mesothelial cells were isolated from patients with liver cirrhosis and pleural effusion, and short-term cultured in RPMI 1640 media containing 10% heat inactivated fetal calf serum and 1% identical supernatant fluid of the patients' transudates. The results obtained are as follows 1. The cultured-reactive mesothelial cells were positive for the protein of cytoskeleton such as cytokeratin and vimentin, but negative for desmin and actin. The resting mesothelial cells showed positive reactions for cylokeratin, but negative for vimentin, desmin and actin. 2. The primary antibodies to the cytokeratin were strongly reactive for CK1, CK8 and CK18 but negative for CK7 and CK19 in both reactive and resting mesothelial cells. 3. Resting mesothelial cells showed negative reactions for CEA, but strong positive reactions in cultured-reactive mesothelial cells. 4. The markers for the monocytes/histiocytes(CD11b, CD14, CD16, CD68, Iysozyme and $\alpha1-antitrypsin$ and $\alpha1-antichymotrypsin$) were nonreactive in resting mesothelial cells, but lysozyme and $\alpha1-antitrypsin$ were weakly reactive in reactive and proliferative mesothelial cells. 5. MHC Class II molecule(HLA-DR antigen) was negative in both resting and reactive mesothelial cells. These results suggest that the short-term cultured, reactive mesothelial cells show a newly aberrant expression of the vimentin and calcine-embryonic antigen. The reason of the aberrant expression of the intermediate filament and oncofetal antigen in reactive and proliferative mesothelial cells should be further evaluated.

• 한국수산과학회지
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• 제33권3호
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• pp.225-229
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• 2000

담수산 rotifer, B. calycinoms를 대상으로 5 종류의 먹이 (ESP, 담수산 농축 Chlonlla, 빵효모 단독구와 $ESP 70{\%}와 담수산 농축 Chlorella 30{\%} 혼합구 및 빵효모 30{\%}와 담수산 농축 Chlorella 70{\%}$ 혼합구)에 대한 내구란 생산과 먹이종류에 따라 생산된 내구란의 부화율을 조사하였다. 먹이 종류에 따른 내구란 생산은 $ESP 70{\%}$와 담수산 농축 Chlorella 30{\%}$ 혼합구와 빵효모 30{\%}$와 담수산 농축 Chlorells 70{\%}$ 혼합구에서 각각 123.3 개/ml와 126.7 개/ml로 가장 높게 나타났고, 단독구보다 혼합구에서 높게 나타났다. 또한 먹이에 따른 내구란의 부화율은 $ESP 70{\%}$와 담수산 농축 Chlorella $30{\%}$ 혼합구에서 $71.9{\%}$로 가장 높았지만 빵효모 $30{\%}$와 담수산 농축 Chlonlla $70{\%}$ 혼합구 및 담수산 농축 Chlorella 단독구와는 유의적인 차이는 보이지 않았다. 본 연구 결과, 담수산 rotifer의 내구란 생산 및 부화율을 높이기 위한 먹이로 담수산 농축 Chlorella에 빵효모와 ESP를 혼합하여 공급하는 것이 가장 효과적인 것으로 판단된다.

• 한국초지조사료학회지
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• 제29권4호
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• pp.355-364
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• 2009

본 연구는 경주마 생산 육성에 중요한 종빈마의 패독과 방목지에 있어 군별 및 개체별 행동양식을 조사하여 경주마 사양관리의 기초자료로 활용하기 위해 실시하였다. 방목 중인 포유 종빈마군에서는 채식이 62%, 기립 휴식이 31%, 음수가 3%, 횡와 휴식이 1%, 보행이 1%, 수유 행동은 2%가 발현되었는데 러닝은 발현되지 않았다. 포유 종빈마 개체별(3필) 행동발현비율의 평균은 채식이 295분으로 55%, 기립 휴식이 193분으로 36%, 보행과 음수가 각각 18분, 15분으로 3%, 수유가 13분으로 2%, 횡와 휴식이 6분으로 1%였다. 개체들은 평균적으로 grooming 11회, 배뇨 2회, 배분 1회의 행동발현이 관찰되었다. 패독에서 관리 중인 임신 종빈마 군에서는 기립 휴식이 48%, 채식 44%, 횡와 휴식 2%, 보행 4%, 음수 2%를 발현하였다. 임신 종빈마 개체별(3필) 발현 비율 평균은 채식 52%, 기립 40%, 보행 6%, 음수 2%였다. 개체들은 평균적으로 grooming 15회, 배뇨 1회, 배분 3회, 투쟁 1회, 구르기 2회의 행동발현이 관찰되었다. 이동패턴의 경우 옆 패독과 인접해 있는 곳에 집중되는 경향을 보였다.

• 대한간호학회지
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• 제25권3호
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• pp.538-548
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• 1995

Exercises are achievement oriented, the process is frequently perceived as hard and difficult Participants drop out from exercise programs in the middle of the training period. Dance movement, which is the deliberate and systematic use of movement, is enjoyable during the movement and provides opportunities for persons to express them-selves. Regular long term dance movement may in-duce a training effect with a decreased drop out rate. Dance movement could be one way to attain wellness, however, there have been few studies to evaluate both physiological and psychological aspects of dance movement. This study focused on evaluating the effects of dance movement training on body weight, resting blood pressure and heart rate, limb circumference and strength, stress response and subjective feelings. This quasi-experimental study was designed as a nonequivalent control group pre test -post test study. Ten healthy fe-male subjects, aged between 19 and 31 years volunteered for an eight week dance movement program. Ten healthy female subjects, between 19 and 21 years of age paticipated as controls. None of the subjects had performed regular physical activity for six months prior to the study. Dance movement was created with reference to Heber's movement guide. The Dance movement program consisted of approximately 30 minutes of dance, three days per week, for eight weeks. During each 30 minute work out, there were approximately 5 minutes of warm-up dancing, 20 minutes of conditioning dance and 5 minutes of cool-down dancing. The intensity for the conditioning phase was at between 60% and 65% of age-adjusted maximum heart rates. Body weight, resting blood pressure and heart rate, circumference of mid upper arm, mid thigh and mid calf, muscle strength of upper and lower limb, physical and psychological response to stress were measured prior to, and following the experimental treatment. Body weight was measured by digital weight scale(Kyung In Corp., Korea). Resting systolic and diastolic blood pressure were measured by sphygmomanometer, Resting heart rate was measured for one minute in a relaxed sitting position using the radial artery. Circumference of mid upper arm, mid thigh and mid calf was determined by tape measure. Muscle strength of the upper extremities was measured by a grip dynamometer (Takei Corp. No.1857, Japan) and that of the extremities was measured by the length of time the leg could be held at 45° Physical and psychological responses to stress were measured using the Symptoms of Stress (SOS)Scale. Paticipants in the dance movement were interviewed by the facilitator following the eight weeks, and their thematic responses about the dance movement were recorded. Following the eight week dance movement train-ing, body weight decreased significantly, circumference of mid thigh and mid calf increased. The length of time leg - raising could be held tended to increase following the dance movement training. Resting systolic and resting heart rate showed a tendency to decrease. Total mean score of stress response tended to de-crease, and mean score of habitual patterns, do-pression, anxiety / fear, anger and cognitive disorganization decreased remarkably following the eight week dance movement. Thematic responses about the dance movement were positive following the training.

• 한국식품영양과학회지
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• 제20권2호
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• pp.94-102
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• 1991

This study was directed to further clarify the effect of resting time or dietary condition for the improvement of exercise capacity and physical fitness, and the changes of some body consitiuents and physiological functions which are related to the exercise metabolism. Sixteen male students(8 athletes and 8 nonathletes)were participated during 3 weeks(Aug. 20-Sep.9, 1989). Each subject performed two treadmill running trials at an absolute intensity (1 mintute in 3.4mph/15% slope and 2 minutes in 5.5mph/20% slope). In the resting time trials, general diet was fed before 1, 2, 4 and 12 hours of the treadmill exercise loaded. in the dietary condition trials, high carbohydrate(HC), high fat(HF) and high protein(HP) diet were fed before 2 hours of the treadmill exercise loaded. Control trial was that of resting time before treadmill exercise loaded after 12 hours of general diet feeding. Measurement were made to study the change of blood glucose, palmitate, lactate, blood pressure and heart rate. The results obtained are summarized as follows: Mean daily metabolizable period. In resting time trial, the blood glucose concentration of athlete and nonathlete from 2 hours group was similar to control group. The blood palmitate concentration of athlete was increased in 1 and 2 hours group but those of nonathlete was not only increased in 1 and2 hours group but was more increased in 12 hours group, compared with both control group. The blood lactate concentration was increased in all experimental group, compared with both control group and those of nonathlete was much higher than athlets. The elevation rate of blood pressure in pre-and after-exercise of athlete was lower than those of nonathlete. In dietary composition trial, the blood glucose concentration of athlete and nonathlete in HC group was higher than other diet groups. The blood lactate concentration of athlete and nonathlete in HC group was lower than other diet groups. There was no remarkable change of the blood palmitate concentration and heart rate in each dietary composition trial, but those of nonathlete was low in HC and high in HP group. In above results, it was suggested that the effective condition of resting time and dietary composition for the improvement of exercise capacity of nonathlete may be 2 hours and HC diet, respectively. But it was showed that the exercise capacity of athlete may not be affected by experimental condition of resting time, except 1 hour after feeding or of dietary composition because of well adaptation in new exercise condition.

• Steel and Composite Structures
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• 제11권5호
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• pp.403-421
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• 2011

In the present manuscript, geometrically nonlinear free vibration analysis of thin laminated plates resting on non-linear elastic foundations is investigated. Winkler-Pasternak type foundation model is used. Governing equations of motions are obtained using the von Karman type nonlinear theory. The method of discrete singular convolution is used to obtain the discretised equations of motion of plates. The effects of plate geometry, boundary conditions, material properties and foundation parameters on nonlinear vibration behavior of plates are presented.