• Proceedings of the Korea Society of Environmental Toocicology Conference
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• 2003.10a
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• pp.179-179
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• 2003

Exposure of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) causes a variety of biological and toxicology effects, most of which are mediated by aryl hydrocarbon receptor (AhR). The ligand-bound AhR as a heterodimer with AhR nuclear translocator (ARNT) binds to its specific DNA recognition site, the dioxin-responsive element (DRE), and it results in increased transcription of CYP1A1 gene. Retinoic acid (RA) regulates the transcription of various genes for several essential functions through binding to two classes of nuclear receptors, the retinoic acid receptor (RAR) and retinoid X receptor (RXR). Constitutive androstane receptor (CAR) also regulates the transcription of gene. In this study, we have examined how RAR, RXR and CAR regulated CYP1A1 in rainbow trout hepatoma cell (RTH 149) using luciferase reporter gene assay system. We did transient transfection with CYP1A1 luciferase reporter gene and treated with TCDD, all-trans RA, 9-cis RA and phenobarbital. Treatment of all-trans RA, 9-cis RA or phenobarbital decreased the TCDD induced transcription of CYP1A1. When we did transient cotransfection with CYP1A1 luciferase reporter gene and RXR, as increase of RXR concentration, the TCDD induced transcription of CYP1A1 was decreased. Transfection with CAR also decreased the TCDD induced transcription of CYP1A1 in RTH 149 cells.

• Development and Reproduction
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• v.23 no.4
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• pp.333-344
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• 2019

In vertebrate reproductive system, estrogen receptor (ER) plays a pivotal role in mediation of estrogenic signaling pathways. In the present study, we report the cDNA cloning, expression analysis, and transcriptional activity of ERβ1 subtype from medaka Oryzias dancena. The deduced O. dancena ERβ1 (odERβ1; 519 amino acids) contained six characteristic A/B to E/F domains with very short activation function 2 region (called AF2). A phylogenetic analysis indicated that odERβ1 was highly conserved among teleost ERβ1 subgroup. A conventional RT-PCR revealed that the odERβ1 transcripts were widely distributed in the multiple tissues, the ovary, brain, gill, intestine, kidney, and muscle. Further, the relatively higher odERβ1 expressions in the ovary and brain were clearly reproduced in RT-qPCR assay. When HA-fused odERβ1 expression vector was transfected into HEK293 cells, an immunoreactivity for odERβ1 was mainly detected in the nucleus part. Finally, an estrogen responsive element driven luciferase reporter assays demonstrated that the transcriptional activity of odERβ1 significantly increased by estradiol-17β (E2) in a dose dependent manner (p<0.05). However, fold-activation of odERβ1 in the presence of E2 was markedly weak, when it compared with those of O. latipes ERβ1. Taken together, these data suggest that odERβ1 represents a functional variant of teleost ERβ subtype and provides a basic tool allowing future studies examining the function of F domain of ERβ1 subtype and expanding our knowledge of ERβ evolution.

• The Korean Journal of Physiology and Pharmacology
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• v.12 no.3
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• pp.95-99
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• 2008

Calcium ($Ca^{2+}$) is an intracellular second messenger associated with neuronal plasticity of the central nervous system. The calcium-binding proteins regulate the $Ca^{2+}$-mediated signals in the cytoplasm and buffer the calcium concentration. This study examined temporal changes of three calcium-binding proteins (calretinin, calbindin and parvalbumin) in the medial vestibular nucleus (MVN) during vestibular compensation after unilateral labyrinthectomy (UL) in rats. Rats underwent UL, and the changes in the expression of these proteins at 2, 6, 12, 24, 48, and 72 h were examined by immuno-fluorescence staining. The expression levels of all three proteins increased immediately after UL and returned to the control level by 48 h. However, the level of calretinin showed changes different from the other two proteins, being expressed at significantly higher level in the contralateral MVN than in the ipsilateral MVN 2 h after UL, whereas the other two proteins showed similar expression levels in both the ipsilateral and contralateral MVN. These results suggest that the calcium binding proteins have some protective activity against the increased $Ca^{2+}$ levels in the MVN. In particular, calretinin might be more responsive to neuronal activity than calbindin or parvalbumin.

• Animal cells and systems
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• v.15 no.3
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• pp.227-233
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• 2011

The Nramp1/Slc11a1 locus encodes a proton-coupled divalent cation transporter, expressed in late endosomes/lysosomes of macrophages, that constitutes a component of the innate immune response to combat intracellular pathogens and it was shown to play an important role in regulating inherent immunity. The previously identified Z-DNA forming polymorphic repeat(GT)n in the promoter region of the human Nramp1 gene does act as a functional polymorphism influencing gene expression. Research has shown that INF-${\gamma}$, TNF-${\alpha}$, IL-$1{\beta}$ and bacteria LPS increase the level of Nramp1 expression. However, the molecular mechanism for Nramp1 gene regulation is unclear. In this research, bovine Nramp1 5'-flanking region (-1748~+769) was cloned and analyzed by bioinformatics. Then to find the core promoter and the cis-acting elements, deletion analysis of promoter was performed using a set of luciferase reporter gene constructs containing successive deletions of the bovine Nramp1 5'-flanking regions. Promoter activity analysis by the dual luciferase reporter assay system showed that the core promoter of Nramp1 was located at +58~-89 bp. Some positive regulatory elements are located at -89~-205 bp and -278~-1495 bp. And the repressor elements were in region -205~-278 bp, intron1 and -1495~-1748 bp. LPS-responsive regions were located at -1495~-1748 bp and -278~-205 bp. The present study provides an initial effort to explore the molecular mechanism of transcriptional activation of the bovine Nramp1 gene and should facilitate further studies to decode the complex regulatory process and for molecular breeding for disease resistance in bovines.

• Journal of Digital Convergence
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• v.12 no.10
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• pp.283-289
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• 2014

Personal rapid transit(PRT) systems have been highlighted in future transportation developments as a result of their potential as sustainable and eco-friendly transport solutions that provide demand-responsive mobility services. One of the most important characteristics of the personal rapid transit system(PRT) is that it can be constructed and operated at a low cost. A fundamental study on the alignment of the PRT guideway considering running stability was conducted in the present study. In addition, a parameter analysis of the major alignment design variables such as curve radius, transition curve length and cant was performed by vehicle dynamic analysis and optimum guideway alignments were proposed. The analysis results suggested that the theoretical values were satisfied and also confirmed the possibility of reducing the standard.

• Smart Structures and Systems
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• v.22 no.4
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• pp.481-493
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• 2018

Ultrasonic guided waves have attracted increasing attention for non-destructive testing (NDT) and structural health monitoring (SHM) of bridge cables. They offer advantages like single measurement, wide coverage of acoustical field, and long-range propagation capability. To design defect detection systems, it is essential to understand how guided waves propagate in cables and how to select the optimal excitation frequency and mode. However, certain cable characteristics such as multiple wires, anchorage, and polyethylene (PE) sheath increase the complexity in analyzing the guided wave propagation. In this study, guided wave modes for multi-wire bridge cables are identified by using a semi-analytical finite element (SAFE) technique to obtain relevant dispersion curves. Numerical results indicated that the number of guided wave modes increases, the length of the flat region with a low frequency of L(0,1) mode becomes shorter, and the cutoff frequency for high order longitudinal wave modes becomes lower, as the number of steel wires in a cable increases. These findings were used in design of transducers for defect detection and selection of the optimal wave mode and frequency for subsequent experiments. A magnetostrictive transducer system was used to excite and detect the guided waves. The applicability of the proposed approach for detecting and locating wire breakages was demonstrated for a cable with 37 wires. The present ultrasonic guided wave method has been found to be very responsive to the number of brokenwires and is thus capable of detecting defects with varying sizes.

• The Transactions of the Korean Institute of Electrical Engineers D
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• v.55 no.11
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• pp.502-510
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• 2006

In this paper, we describe implementation of a computer access device for the severly motor-disability. Many people with severe motor disabilities need an augmentative communication technology. Those who are totally paralyzed, or 'locked-in' cannot use conventional augmentative technologies, all of which require some measure of muscle control. The forehead is often the last site to suffer degradation in cases of severe disability and degenerative disease. For example, In ALS(Amyotrophic Lateral Sclerosis) and MD(Muscular dystrophy) the ocular motorneurons and ocular muscles are usually spared permitting at least gross eye movements, but not precise eye pointing. We use brain and body forehead bio-potentials in a novel way to generate multiple signals for computer control inputs. A bio-amplifier within this device separates the forehead signal into three frequency channels. The lowest channel is responsive to bio-potentials resulting from an eye motion, and second channel is the band pass derived between 0.5 and 45Hz, falling within the accepted Electroencephalographic(EEG) range. A digital processing station subdivides this region into eleven components frequency bands using FFT algorithm. The third channel is defined as an Electromyographic(EMG) signal. It responds to contractions of facial muscles and is well suited to discrete on/off switch closures, keyboard commands. These signals are transmitted to a PC that analyzes in a time series and a frequency region and discriminates user's intentions. That software graphically displays user's bio-potential signals in the real time, therefore user can see their own bio-potentials and control their physiological signals little by little after some training sessions. As a result, we confirmed the performance and availability of the developed system with experimental user's bio-potentials.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.27 no.3
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• pp.617-636
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• 2017

Many businesses have adopted the standard security management structure such as ISO27001 and K-ISMS for strengthening business's security management structure to protect their core information assets and have acquired partial output from such effort. However, many risk factors such as recent advances in Information Technology and evolution of intrusion methods have increased exponentially requiring the businesses to response even more quickly with better accuracy. For such purpose, a study of 'Real Time Security Management Structure for Business' based on security management process optimization, defining a set of security index for managing core security area and calculation of risk indices for precognition of intrusion risk area has been made. Also, a survey on opinions of an expert panel has been conducted. The effectiveness of studied structure was analyzed using AHP method as well. Using this study, security personnels of a company can improve efficiency of the preemptive responsive and quicker measure from the current security management structure.

• Journal of Korean Society of Transportation
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• v.27 no.5
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• pp.63-74
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• 2009

One of the effective countermeasures for preventing traffic accidents is to provide traffic safety warning information to drivers. Provision of warning information would lead to safer driving to avoid accident occurrence. This study investigated the effects of in-vehicle warning information on driver's behavior. A variety of warning information contents using text, sound, and pictograms were prepared for the field experiments. Individual vehicle speed and acceleration data, which represent quantitative drivers' behavior in response to in-vehicle warning information, were collected using differential global positioning systems (DGPS). Statistical analyses including ANOVA and Tukey's pairwise comparison were conducted. It is expected that the results could be invaluable for designing more effective warning information.

• Genes and Genomics
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• v.40 no.11
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• pp.1199-1211
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• 2018

Soil acidification is one of major problems limiting crop growth and especially becoming increasingly serious in China owing to excessive use of nitrogen fertilizer. Only the STOP1 of Arabidopsis was identified clearly sensitive to proton rhizotoxicity and the molecular mechanism for proton toxicity tolerance of plants is still poorly understood. The main objective of this study was to investigate the transcriptomic change in plants under the low pH stress. The low pH as a single factor was employed to induce the response of the wheat seedling roots. Wheat cDNA microarray was used to identify differentially expressed genes (DEGs). A total of 1057 DEGs were identified, of which 761 genes were up-regulated and 296 were down-regulated. The greater percentage of up-regulated genes involved in developmental processes, immune system processes, multi-organism processes, positive regulation of biological processes and metabolic processes of the biological processes. The more proportion of down-regulation genes belong to the molecular function category including transporter activity, antioxidant activity and molecular transducer activity and to the extracellular region of the cellular components category. Moreover, most genes among 41 genes involved in ion binding, 17 WAKY transcription factor genes and 17 genes related to transport activity were up-regulated. KEGG analysis showed that the jasmonate signal transduction and flavonoid biosynthesis might play important roles in response to the low pH stress in wheat seedling roots. Based on the data, it is can be deduced that WRKY transcription factors might play a critical role in the transcriptional regulation, and the alkalifying of the rhizosphere might be the earliest response process to low pH stress in wheat seedling roots. These results provide a basis to reveal the molecular mechanism of proton toxicity tolerance in plants.