• Asian-Australasian Journal of Animal Sciences
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• 제31권11호
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• pp.1738-1746
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• 2018

Objective: The aim of the experiment was to assess the effect of increasing amounts of Leucaena leucocephala forage on dry matter intake (DMI), organic matter intake (OMI), enteric methane production, rumen fermentation pattern and protozoa population in cattle fed Pennisetum purpureum and housed in respiration chambers. Methods: Five crossbred heifers (Bos taurus${\times}$Bos indicus) (BW: $295{\pm}6kg$) were fed chopped P. purpureum grass and increasing levels of L. leucocephala (0%, 20%, 40%, 60%, and 80% of dry matter [DM]) in a $5{\times}5$ Latin square design. Results: The voluntary intake and methane production were measured for 23 h per day in respiration chambers; molar proportions of volatile fatty acids (VFAs) were determined at 6 h postprandial period. Molar concentration of VFAs in rumen liquor were similar (p>0.05) between treatments. However, methane production decreased linearly (p<0.005), recording a maximum reduction of up to ~61% with 80% of DM incorporation of L. leucocephala in the ration and no changes (p>0.05) in rumen protozoa population were found. Conclusion: Inclusion of 80% of L. leucocephala in the diet of heifers fed low-quality tropical forages has the capacity to reduce up to 61.3% enteric methane emission without affecting DMI, OMI, and protozoa population in rumen liquor.

• 대한화학회지
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• 제63권4호
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• pp.253-259
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• 2019

5-Aminoimidazole-4-carboxamide ribonucleotide (AICAR), a structural analog of adenosine monophosphate (AMP), promotes oxidative remodeling in muscle cells. AICAR activates AMP-dependent protein kinase (AMPK), thus increasing lipid metabolism, respiration, and mitochondrial counts. This process is called oxidative remodeling, which enhances the physical endurance of mice. To test this drug on an invertebrate that is genetically similar to humans, we used the small water crustacean Daphnia magna, which is sensitive to changes in water conditions. We tested the effects of pH on the efficacy of AICAR using two methods. One method measured oxygen consumption of Daphnia in oxygen chambers. The other method determined lipid levels of Daphnia through fluorescent tagging of lipids. The results showed that when exposed to AICAR at pH 6.58, D. magna consumed more oxygen and had lower overall levels of lipids, which is consistent with the expected effects of AICAR, such as increased respiration and lipid metabolism.

• Asian-Australasian Journal of Animal Sciences
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• 제13권3호
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• pp.307-312
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• 2000

Fasting heat production (FHP) of growing buffalo calves (Bubalus bubalis) in the body weight range of 76 to 236 kg was determined using open circuit respiration chamber. The details of the chambers, calibration of gas analysers and operation of the systems are described. Animals were fasted for 96 hrs during which only water was provided. FHP was determined during next 24 hrs. The mean oxygen consumed, carbon dioxide and methane produced and urinary N excretion per 24 h was $17.03{\ell}$, $11.70{\ell}$, and $0.12{\ell}$ and 0.35 g respectively. The mean respiratory quotient ranged from 0.68 to 0.71, which indicated that post absorptive stage is reached after 96 hrs in growing buffalo calves previously fed ammoniated straw-based ration. Mean FHP of calves was $331.4kJ/kg\;W^{0.75}$. FHP of calves with range of mean body weights of 167 to 235 kg, although nonsignificant but, was almost 12% higher than of calves having mean body weight of 101 kg. Suitable exponent to body weight to describe FHP of buffalo calves was 0.87.

• Nuclear Engineering and Technology
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• 제54권12호
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• pp.4644-4651
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• 2022

Due to its short half-life, thoron gas has been assumed to have negligible health hazards on humans compared to radon. But, one of the decay products with a long half-life can make it to be transported to a long distance and to cause a severe internal dose through respiration. Since most commercial radon detectors can not discriminate thoron signals from radon signals, it is very common to overestimate radon doses which in turn result in biased estimation of lung cancer risk in epidemiological studies. Though some methods had been suggested to measure thoron and radon separately, they could not be used for real-time measurement because of CR-39 or LR-115. In this study, an effective method was suggested to measure radon and thoron separately from the free air. It was observed that the activity of thoron decreases exponentially due to delay time caused by a long pipe between two chambers. Therefore from two ion chambers apart in time, it was demonstrated that thoron and radon could be measured separately and simultaneously. We also developed a collimated alpha source and with this source and an SBD, we could convert the ion chamber reading to count rate in cps.

• 환경생물
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• 제32권4호
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• pp.389-394
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• 2014

본 연구는 몬순기후에 의해 장마라는 특징적인 강우패턴을 가지는 환경조건에서 강우 패턴 및 강우 강도의 변화가 삼림 내 지표 유출수와 토양수분함량을 변화시켜 토양호흡의 배출 양상을 어떻게 변화시키는지 알아보기 위하여 수행되었다. 대상지는 경기도 광주시에 위치한 서울대학교 학술림 내 잣나무식재림으로 2012년 1년 동안 자동 토양호흡 쳄버를 이용하여 연변화를 측정하였고 단근처리 기법을 이용하여 토양호흡 중 약 24%가 뿌리호흡에서 기인한 것으로 추정되었다. 이와 더불어 장마와 태풍으로 인한 다양한 강도의 강우가 발생한 7월 말부터 9월 말까지 휴대용 토양호흡 측정기를 이용하여 관측지 내 단근처리구와 단근 및 강우차단처리구에서 종속영양생물호흡을 측정하였다. 토양수분함량의 경우 고강도의 강우가 며칠씩 이어지더라도 20%를 초과하지 않았다. 이는 강수가 상대적으로 적은 시기에는 수목활성이나 토양미생물의 활성에 영향을 미칠 것으로 추정된다. 강우 강도가 10 mm 이하의 저강도 강우에서는 종속영양생물호흡이 약 14.4% 상승하였으나 10 mm 이상의 고강도 강우조건에서는 종속영양생물호흡이 25.5% 감소하였다. 이는 강우 강도에 따라 종속영양생물호흡의 배출 양상이 달라짐을 보여주었다.

• 한국농림기상학회지
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• 제9권3호
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• pp.203-208
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• 2007

본 연구에서는 동시에 여러 지점을 측정할 수 있는 open flow 시스템을 이용하여 소나무 줄기에서 발생하는 호흡을 겨울과 여름 두 시기에 측정하였다. 연구결과, 줄기의 목부조직에서 호흡속도($R_{stem}$)는 겨울에 $0.25-0.55{\mu}mol\;m^{-2}s^{-1}$, 여름에 $1.25-1.63{\mu}mol\;m^{-2}s^{-1}$의 범위를 나타냈다. 시기별 측정된 수목(15개체)간 $R_{stem}$의 변화성은 변동계수로 나타낼 수 있으며, 그 결과 여름과 겨울이 모두 23%로 시기에 따른 차이를 보이지 않았다. 이러한 변동계수 값에 근거하면 $R_{stem}$ 이 95% 신뢰수준에서 실제 값과의 차이가 10%와 20%보다 적기 위해서는 샘플링 수가 각각 최소 24개와 6개가 필요한 것으로 확인되었다. 또한 겨울과 여름의 온도와 개체간 평균 $R_{stem}$의 상관관계에서, $Q_{10}$ 값은 겨울이 1.49, 여름이 1.45로 계절간에 큰 차이를 나타내지 않았다. 그러나 $R_{20}$(줄기 온도 $20^{\circ}C$일 때의 $R_{stem}$)은 겨울($0.61{\mu}mol\;m^{-2}s^{-1}$)보다 여름($1.23{\mu}mol\;m^{-2}s^{-1}$)이 두 배 정도 컸다.

• 한국동물생명공학회지
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• 제34권3호
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• pp.173-182
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• 2019

This study was performed to evaluate the effect of heat stress on the status of physiological responses, blood parameter, serum T3 and cortisol, and heat shock proteins (HSP 27, 70, and 90) of Hanwoo cattle. Six Hanwoo steers (242.8 ± 7.2 kg of BW) were housed in the climate-controlled respiration chambers. The experiment consisted of 7 days (control; 0 day) at thermoneutral (air temperature (Ta) of 15℃ and relative humidity (RH) of 60%; temperature-humidity index (THI) = 64), and by 3 and 6 days (treatment groups) at heat stress (Ta of 35℃ and RH of 60%; THI = 87). Body temperature of each parts (frank, rump, perineum and foot) and rectal temperature elevated in heat stress groups (3 days and 6 days) than the control group (0 day). Respiration rates increased in 3 days and 6 days (88.5 ± 0.96 bpm and 86.3 ± 0.63 bpm, respectively) from 0 days (39.5 ± 0.65 bpm). Feed intake significantly decreased in heat stress groups (3 days and 6 days, 3.7 ± 0.14 kg and 4.0 ± 0.15 kg, respectively) than the control group (0 day, 5.0 ± 0.00 kg). In addition, final BW significantly decreased in heat stress groups (3 days and 6 days, 211.8 ± 4.75 kg and 215.5 ± 3.50 kg, respectively) than the control group (0 day, 240.0 ± 25.00 kg). However, heat stress has no significant effect on blood parameter, serum T3 and cortisol. Nevertheless, heat stress increased HSPs mRNA expression in liver tissue, and serum concentration of HSPs. Despite Hanwoo cattle may have high adaptive ability to heat stress, our results suggested that heat stress directly effect on body temperature and respiration rate as well as serum and tissue HSPs. Therefore, we are recommended that HSPs could be the most appropriate indicators of Hanwoo cattle response to heat stress.

• 한국양식학회지
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• 제9권2호
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• pp.179-186
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• 1996

실험실에서 부화시킨 도화새우의 zoea 유생발달에 따른 섭식, 성장, 탈피 및 대사에 관한 에너지수지를 분석하였다. 도화새우의 zoea 전 유생기간중 섭식에 의한 총에너지는 140.88 J이었다. 이중 체성장에 사용된 에너지는 17.07 J이었고 호흡, 탈피 및 배설에 사용된 에너지는 각각 16.22 J, 1.19 J 및 106.40 J이었다. 섭식한 먹이에 대한 동화효율(assimilation efficiency, A/I)은 $24.47\%$였다. 성장으로 전환된 에너지의 총섭식에너지에 대한 백분률인 총성장효율(gross growth efficiency, $K_1$)과 성장으로 전환된 동화에너지에 대한 백분률인 순성장효을 (net growth efficiency, $K_2$)은 각각 $12.96\%$와 $52.96\%$ 였다. 동화에너지가 체성장과 대사에너지에 사용된 것은 각각 $49.51\%$와 $47.04\%$였다.

• Journal of Ecology and Environment
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• 제42권4호
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• pp.272-277
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• 2018

Background: Studying the ecosystem carbon cycle requires analysis of interrelationships between soil respiration (Rs) and the environment to evaluate the balance. Various methods and instruments have been used to measure Rs. The closed chamber method, which is currently widely used to determine Rs, creates a closed space on the soil surface, measures $CO_2$ concentration in the inner space, and calculates Rs from the increase. Accordingly, the method is divided into automatic or manual chamber methods (ACM and MCM, respectively). However, errors of these methods and differences in instruments are unclear. Therefore, we evaluated the characteristics and difference of Rs values calculated using both methods with actual data. Results: Both methods determined seasonal variation patterns of Rs, reflecting overall changes in soil temperature (Ts). ACM clearly showed detailed changes in Rs, but MCM did not, because such small changes are unknown as Rs values are collected monthly. Additionally, Rs measured using MCM was higher than that using ACM and differed depending on measured plots, but showed similar tendencies with all measurement times and plots. Contrastingly, MCM Rs values in August for plot 4 were very high compared with ACM Rs values because of soil disturbances that easily occur during MCM measurements. Comparing Rs values calculated using monthly means with those calculated using MCM, the ACM calculated values for monthly averages were higher or lower than those of similar measurement times using the MCM. The difference between the ACM and MCM was attributed to greater or lesser differences. These Rs values estimated the carbon released into the atmosphere during measurement periods to be approximately 57% higher with MCM than with ACM, at 5.1 and $7.9C\;ton\;ha^{-1}$, respectively. Conclusion: ACM calculated average values based on various Rs values as high and low for measurement periods, but the MCM produced only specific values for measurement times as representative values. Therefore, MCM may exhibit large errors in selection differences during Rs measurements. Therefore, to reduce this error using MCM, the time and frequency of measurement should be set to obtain Rs under various environmental conditions. Contrastingly, the MCM measurement is obtained during $CO_2$ evaluation in the soil owing to soil disturbance caused by measuring equipment, so close attention should be paid to measurements. This is because the measurement process is disturbed by high $CO_2$ soil concentration, and even small soil disturbances could release high levels into the chamber, causing large Rs errors. Therefore, the MCM should be adequately mastered before using the device to measure Rs.

• 한국농림기상학회지
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• 제7권1호
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• pp.107-114
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• 2005

토양 호흡은 주요 육상 생태계의 탄소 순환을 이해하는데 중요한 성분 중 하나이다. 토양 호흡 관측에서 쳄버 방법은 가장 일반적인 방법이다. 다양한 토양 호흡쳄버 방법에 의해 이루어지는 관측 값들을 비교하기 위해서는, 측기의 특성에 따른 오차를 파악하는 것이 필요하다. 이를 위하여 온도 조절이 가능한 식물 환경 조절 시스템에서 네 개의 폐회로 역학 쳄버와 자동 개폐 쳄버를 비교하였다. 식물 환경 조절 시스템의 내부의 이산화탄소 농도의 변동과 토양 수분의 변동으로 어느 정도의 오차가 포함된 결과이지만, 실험을 통해 토양 온도와 이산화탄소 농도의 변화에 따른 대한 네 측기들의 반응 특성과 실내 비교 실험의 계획과 방법에 대한 중요한 교훈을 얻을 수 있었다.