• Journal of the Korean Wood Science and Technology
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• 제37권4호
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• pp.330-339
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• 2009

본 연구에서는 삼나무 재색제어에 적합한 적정 열처리 조건을 구명하고, 열처리에 따른 다양한 재질변화를 평가하고자 하였다. 국산 삼나무에 대한 온도제어 열처리를 통하여, $170^{\circ}C$ 이상의 열처리 조건에서 심변재 사이의 재색차이가 줄어드는 경향을 확인하였으며, 이러한 경향은 처리시간이 증가함에 따라 더 크게 나타났다. 열처리재의 평형함수율이 무처리재에 비해 50% 정도 낮아 목재의 사용과정에서 나타나는 수분에 의한 성능저하 문제를 예방할 수 있을 것으로 기대되며, 열처리가 부후균에 대한 내후성 증가에도 영향하는 것으로 확인됨에 따라 친환경 방부처리 기술로써 열처리의 적용에 대한 본격적인 연구가 필요할 것으로 판단된다. 열처리재의 역학성능은 무처리재에 비해 대체로 증가하는 것으로 나타났으나, 연성이 감소함으로 인하여 충격휨흡수에너지는 크게 감소하는 결과를 보여 열처리재의 용도를 결정하는데 있어서 이러한 물성 변화가 고려되어야 할 것이다. 열처리에 의한 물성변화의 원인을 규명하기 위하여 미세구조의 변화를 관찰하였으나 특별한 차이는 나타나지 않았다. 따라서 화학성분의 변화를 분석함으로써 물성변화의 원인을 규명하는 추가적인 연구가 필요할 것으로 여겨진다.

• Animal Bioscience
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• 제37권2호
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• pp.193-202
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• 2024

Objective: Oxidative stress (OS) is a pathological process arising from the excessive production of free radicals in the body. It has the potential to alter animal gene expression and cause damage to the jejunum. However, there have been few reports of changes in the expression of long noncoding RNAs (lncRNAs) in the jejunum in piglets under OS. The purpose of this research was to examine how lncRNAs in piglet jejunum change under OS. Methods: The abdominal cavities of piglets were injected with diquat (DQ) to produce OS. Raw reads were downloaded from the SRA database. RNA-seq was utilized to study the expression of lncRNAs in piglets under OS. Additionally, six randomly selected lncRNAs were verified using quantitative real-time polymerase chain reaction (qRT-PCR) to examine the mechanism of oxidative damage. Results: A total of 79 lncRNAs were differentially expressed (DE) in the treatment group compared to the negative control group. The target genes of DE lncRNAs were enriched in gene ontology (GO) terms and Kyoto encyclopedia of genes and genomes (KEGG) signaling pathways. Chemical carcinogenesis-reactive oxygen species, the Foxo signaling pathway, colorectal cancer, and the AMPK signaling pathway were all linked to OS. Conclusion: Our results demonstrated that DQ-induced OS causes differential expression of lncRNAs, laying the groundwork for future research into the processes involved in the jejunum's response to OS.

• Mycobiology
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• 제46권4호
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• pp.382-387
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• 2018

The entomopathogenic fungus Cordyceps militaris is a valuable medicinal ascomycete, which degenerates frequently during subsequent culture. To avoid economic losses during industrialized production, scratching stimuli of mycelia was introduced to improve the fruiting body production. The present results indicated that higher yields and biological efficiency were obtained from two degenerate strains (YN1-14 and YN2-7) but not from g38 (an insertional mutant in Rhf1 gene with higher yields and shorter growth periods). Furthermore, the growth periods of the fruiting bodies were at least 5 days earlier when the mycelia were scratched before stromata differentiation. Three ROS-scavenging genes including Cu/Zn superoxide dismutase (CmSod1), Glutathione peroxidase (CmGpx), and Catalase A (CmCat A) were isolated and their expression profiles against scratching were determined in degenerate strain YN1-14 and mutant strain g38. At day 5 after scratching, the expression level of CmGpx significantly decreased for strain g38, but that of CmSod1 significantly increased for YN1-14. These results indicated that scratching is an effective way to promote fruiting body production of degenerate strain, which may be related at least with Rhf1 and active oxygen scavenging genes.

• 한국지반환경공학회 논문집
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• 제23권12호
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• pp.41-53
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• 2022

산업화가 시작된 후 인류는 더 많은 자원을 필요로 하고 소비하며, 현재 자원 고갈은 지구의 심각한 문제이다. 그러나 우주에는 무한한 자원이 존재한다. 특히 달은 지구와 가장 가까운 행성으로 미래 인류에게 필요한 헬륨-3, 희토류 등 많은 자원을 보유하고 있다. 이러한 자원을 채굴하기 위해서는 인류가 달에 상주할 필요가 있다. 그리고 달에 거주하는 인류는 식량, 건축, 산업 자재 등 많은 자원이 필요하다. 그러나 이러한 자원을 지구에서 달까지 운반하려면 천문학적인 비용이 소모되므로 현지에서 자원을 조달하여 인류의 활동을 지원하는 연구가 진행되고 있다. 이를 ISRU(In-Situ Resources Utilization)라고 하며, 이는 우주개발에 필수적이다. 본 논문에서는 ISRU이 필수적인 이유와 그 기술현황을 소개하고 향후 연구과제에 대해 서술하였다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 추계학술대회
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• pp.101-101
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• 2018

Resveratrol is a polyphenolic compound, which is a naturally occurring phytochemical and is found in a variety of plants, including food such as grapes, berries and peanuts. Although several studies have demonstrated that resveratrol possesses anti-cancer activity against various types of human cancer, the molecular mechanisms of resveratrol-mediated overcome drug resistance potential are unclear. In this study, we determined whether resveratrol attenuates drug resistance responses in 5-fluorouracil-resistant colon cancer (SNUC5/5-FUR) cells. Treatment with resveratrol significantly enhanced apoptosis in a concentration-dependent manner, which was associated with the modulation of anti- and/or pro-apoptotic protein expression, activation of caspases and activation of AMP-activated protein kinase. Resveratrol treatment also increased the induction of autophagy through up-regulation of autophagy-related genes such as Microtubule-associated protein 1A/1B-light chain 3, P62 and beclin-1. However, blocking of autophagy by bafilomycin A1 reduced apoptotic cell death, suggesting that resveratrol-induced autophagy functions as a cell death mechanism in SNUC5/5-FU cells. Although the further studies are needed, these findings suggest that resveratrol may have therapeutic potential to overcome drug resistance in colon cancer patients.

• Asian Pacific Journal of Cancer Prevention
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• 제14권10호
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• pp.5599-5608
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• 2013

With the high morbidity and mortality caused by cancer, finding new and more effective anti-cancer drugs is very urgent. In current research, biotransformation plays a vital role in the research and development of cancer drugs and has obtained some achievements. In this review, we have summarized four applications as follows: to exploit novel anti-cancer drugs, to improve existing anti-cancer drugs, to broaden limited anti-cancer drug resources and to investigate correlative mechanisms. Three different groups of important anti-cancer compounds were assessed to clarify the current practical applications of biotransformation in the development of anti-cancer drugs.

• Journal of Microbiology and Biotechnology
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• 제34권6호
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• pp.1249-1259
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• 2024

It remains to be determined whether there is a geographical distribution pattern and phylogenetic signals for the Mycena strains with seed germination of the orchid plant Gastrodia elata. This study analyzed the community composition and phylogenetics of 72 Mycena strains associated with G. elata varieties (G. elata. f. glauca and G. elata. f. viridis) using multiple gene fragments (ITS+nLSU+SSU). We found that (1) these diverse Mycena phylogenetically belong to the Basidiospore amyloid group. (2) There is a phylogenetic signal of Mycena for germination of G. elata. Those strains phylogenetically close to M. abramsii, M. polygramma, and an unclassified Mycena had significantly higher germination rates than those to M. citrinomarginata. (3) The Mycena distribution depends on geographic site and G. elata variety. Both unclassified Mycena group 1 and the M. abramsii group were dominant for the two varieties of G. elata; in contrast, the M. citrinomarginata group was dominant in G. elata f. glauca but absent in G. elata f. viridis. Our results indicate that the community composition of numerous Mycena resources in the Zhaotong area varies by geographical location and G. elata variety. Importantly, our results also indicate that Mycena's phylogenetic status is correlated with its germination rate.

• Asian-Australasian Journal of Animal Sciences
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• 제32권10호
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• pp.1501-1510
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• 2019

Objective: An experiment was conducted to evaluate genetic diversity of 26 Chinese indigenous goats by 30 microsatellite markers, and then to define conservation priorities to set up the protection programs according to the weight given to within- and between-breed genetic diversity. Methods: Twenty-six representative populations of Chinese indigenous goats, 1,351 total, were sampled from different geographic regions of China. Within-breed genetic diversity and marker polymorphism were estimated calculating the mean number of alleles, observed heterozygosities, expected heterozygosities, fixation index, effective number of alleles and allelic richness. Conservation priorities were analyzed by statistical methods. Results: A relatively high level of genetic diversity was found in twenty-four population; the exceptions were in the Daiyun and Fuqing goat populations. Within-breed kinship coefficient matrices identified seven highly inbred breeds which should be of concern. Of these, six breeds receive a negative contribution to heterozygosity when the method was based on proportional contribution to heterozygosity. Based on Weitzman or Piyasatian and Kinghorn methods, the breeds distant from others i.e. Inner Mongolia Cashmere goat, Chengdu Brown goat and Leizhou goat obtain a high ranking. Evidence from Caballero and Toro and Fabuel et al method prioritized Jining Gray goat, Liaoning Cashmere goat, and Inner Mongolia Cashmere goat, which agree with results from Kinship-based methods. Conclusion: Conservation priorities were determined according to multiple methods. Our results suggest Inner Mongolia Cashmere goat (most methods), Jining Gray goat and Liaoning Cashmere goat (high contribution to heterozygosity and total diversity) should be prioritized based on most methods. Furthermore, Daiyun goat and Shannan White goat also should be prioritized based on consideration of effective population size. However, if one breed can continually survive under changing conditions, the straightforward approach would be to increase its utilization and attraction for production via mining breed germplasm characteristics.

• 자원리싸이클링
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• 제23권2호
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• pp.81-89
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• 2014

화력발전소 석탄재는 2010년을 기준으로 약 850만톤, 연탄재는 약 74만톤 배출된다. 이는 대량 재활용되지 않아 경제적, 환경적으로 부담이 되고 있는 실정이므로, 석탄 연소부산물의 자원화기술개발이 시급하다. 석탄 연소부산물을 균질하게 가공하는 분쇄 및 선별 등의 자원처리기술을 적용하고, 석탄 연소부산물의 품질을 관리하여, 비소성 그린시멘트인 지오폴리머의 원료로 재활용하고자 본 연구에서는 석탄 연소부산물의 지오폴리머 원료화 기술에 대한 특허와 논문을 분석하였다. 분석범위는 1979년 ~ 2013년까지의 미국, 유럽연합, 일본, 한국의 등록/공개된 특허와 SCI 논문으로 제한하였다. 특허와 논문은 키워드를 사용하여 수집하였으며, 기술의 정의에 의해 필터링하였다. 특허와 논문의 동향은 연도, 국가, 기업, 기술 등에 따라 분석하여 고찰하였다.

• Geomechanics and Engineering
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• 제23권1호
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• pp.85-92
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• 2020

This study adopted soil test and laboratory physical model experiments to simulate the tailings impoundment accumulation process according to the principle of similarity. Relying on the practical engineering, it analyzed the tailings deposition characteristics on dry beach surface during the damming process, as well as the variation rules of dam saturation line. Results suggested that, the tailings particles gradually became finer along the dry beach surface to inside the impoundment. The particle size suddenly changed at the junction between the deposited beach and the water surface, which displayed an obvious coarsening phenomenon. Besides, the deposited beach exhibited the vertical feature of coarse upward and fine downward on the whole. Additionally, in the physical model, the saturation line elevated with the increase in dam height, and its amplitude was relatively obvious within the range of 1.0-4.5 m away from the initial dam. Under flood condition, the saturation line height was higher than that under normal condition on the whole, with the maximum height difference of 4 cm. This study could provide an important theoretical basis for further studies on dam failure experiments and the evolution rules of leaked tailings flow.