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Studies on the disease resistance of tobacco varieties to Black shank, Black root rot Wild fire, Brown spot, CMV-Y and Blue mold -Disease resistance of Korean native varieties- (담배중요병해저항성에 관한 연구 특히 한국재래종에 대하여)

  • Il Hou
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.4 no.1
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    • pp.103-108
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    • 1968
  • Resistance to various diseases of Korean native varieties were investigated in field. The results obtained are summarized as follows: 1) The varieties Ubangtchio and Osib-eubthio were moderately resistant to black shank, but Suantchio, Usultchio and Gwangtchio were slightly resistant. Dixie Bright 101, $H_2$ and Bright Yellow 4 were more resistant to black shank than any of the Korean native varieties. 2) Mokgitchio, Ubangtchio and Osib-eubtchio were moderately resistant to black root rot, but Useultchio Suantchio and Hoetchio were susceptible than Others. 3) Muktchio, Hoetchio and Ubangtchio were slightly resistant to wild fire. 4) Osib-ebthio was highly resistant to brown spot, but Ubangtchio, Hyangtchio and Mokgitchio were moderately resistant and Useultchio was susceptible to brown spot, but it was resistant than Bright ellow 4 or Bernhart 1000-1. 5) Hyangtchio was slightly resistant to CMV-Y. According to these results, except Virus disease, Ubangtchio was resistant to all of the above mentioned diseases. Osib-eubtchio, Hoetchio, Hyangtchio and Suantchio showed proferable disease resistant and these might be well utilized as breeding materials.

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Prevalence of chloramphenicol-resistant gene in Escherichia coli from water sources in aquaculture farms and rivers of Kuching, Northwestern Borneo

  • Leong, Sui Sien;Lihan, Samuel;Toh, Seng Chiew
    • Fisheries and Aquatic Sciences
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    • v.25 no.4
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    • pp.202-213
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    • 2022
  • Antibiotic resistant Escherichia coli cases are increasing high especially in Southeast Asia. Illegal use of the antibiotic in the aquaculture farming may become the culprit of the outbreak and spread into environmental source. A study was conducted to: 1) detect the chloramphenicol (CAL)-resistant gene in E. coli isolated from three aquaculture farms and six rivers of northwestern Borneo and 2) investigate the correlation between cat gene with five common antibiotics used. Isolation of E. coli was done on Eosin methylene blue agar and characterized using indole, methyl red, Voges-Proskauer, citrate tests. E. coli isolates were subsequently tested for their susceptibility to five antibiotics commonly used in aqua-farming. The CAL-resistant E. coli were further analyzed for the presence of resistant genes (cat I, cat II, cat III, cat IV) using multiplex polymerase chain reaction. 42 bacterial colonies were isolated from a total of 80 individual water samples, 34 of which were identified as E. coli. Result showed 85.3% of the E. coli isolates were resistant to amoxicillin, 35.3% were resistant to tetracycline, 29.4% were resistant to CAL, 17.6% were resistant to nitrofurantoin and 8.8% were resistant to nalidixic acid. All of the 10 CAL resistant E. coli isolateswere detected with cat II genes; five isolates detected with cat IV genes; three isolates detected with cat III genes; and another two detected with cat I genes. Pearson correlation coefficient shows highly significant relationship between resistance pattern of CAL with amoxicillin; and CAL with tetracycline. Our findings provide the supplementary information of the CAL resistance gene distribution, thereby improving our understanding of the potential risk of antibiotic resistance underlying within this microbial ecosystem.

형질전환 연초의 복합바이러스 저항성

  • 이기원;채순용;이청호;이영기;강신웅;박성원;박은경
    • Journal of the Korean Society of Tobacco Science
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    • v.21 no.1
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    • pp.70-76
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    • 1999
  • KF 116 was TMV resistant tobacco plant and KB 301 was PVY resistant plant transformed with TMV CP gene and PVY CP gene, respectively. These resistant plants were cross-fertilized and the 4 lines of the TMV-PVY resistant plants were selected from F1 hybrid plants. The rate of PVY-resistant plant in these hybrids was 100 percent and that of TMV-resistant plants including delay type was 90-98 percent at 4 weeks after virus inoculation. It was confirmed that the TMV and PVY CP genes were integrated into the genome of hybrid plants by genomic PCR, and Southern blot hybridization. The genome of F1 hybrid plants had one copy and 4 copies of PVY-CP gene and TMV-CP gene, respectively, and CaMV 35S promoters were not methylated, regardless of the difference symptom development to TMV.

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Medical Management of Drug-Resistant Tuberculosis

  • Jeon, Doosoo
    • Tuberculosis and Respiratory Diseases
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    • v.78 no.3
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    • pp.168-174
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    • 2015
  • Drug-resistant tuberculosis (TB) is still a major threat worldwide. However, recent scientific advances in diagnostic and therapeutic tools have improved the management of drug-resistant TB. The development of rapid molecular testing methods allows for the early detection of drug resistance and prompt initiation of an appropriate treatment. In addition, there has been growing supportive evidence for shorter treatment regimens in multidrug-resistant TB; and for the first time in over 50 years, new anti-TB drugs have been developed. The World Health Organization has recently revised their guidelines, primarily based on evidence from a meta-analysis of individual patient data (n=9,153) derived from 32 observational studies, and outlined the recommended combination and correct use of available anti-TB drugs. This review summarizes the updated guidelines with a focus on the medical management of drug-resistant TB.

Susceptibility of Escherichia coli Isolated from Cattle to Some Antimicrobial Agents (소에서 분리(分離)한 Escherichia coli의 항생물질내성(抗生物質耐性) 및 전달성내성인자(傳達性耐性因子)의 분포(分布))

  • Park, Cheong Kyu
    • Korean Journal of Veterinary Research
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    • v.17 no.1
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    • pp.5-8
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    • 1977
  • One hundred and fifty seven Escherichia coli strains isolated from 18 cattle (9 dairy cattle received penicillin, streptomycin (SM) or sulfadimethoxine for treatment of diseases and 9 Korean native cattle not received antibiotics) were studied for the drug resistance and distribution of R factors. Of 88 E. coli strains isolated from cattle not received antibiotics, only 1 strain was resistant to SM, but about 46 per cent of 69 E. coli strains isolated from cattle received antibiotics were resistant to SM, tetracycline (TC), ampicillin (AP), kanamycin (KM), chloramphenicol (CM), and sulfisomidine (Su), alone or in combination thereof. Of resistant strains, about 72% were resistant to three or more antibiotics, but 28% were found to singly resistant. The most frequent resistant pattern was triple resistance to AP, KM and Su (37.6%), and quadruple one to SM, TC, CM and Su (12.5%). About 28% of resistant strains carried R factors which were transferable to E. coli ML 1410 $NA^r$ by conjugation.

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Screening Resistant Red Pepper Varieties to Meloidogyne hapla and their Resistance Mechanisms (당근뿌리혹선충(Meloidogyne hapla)에 대한 저항성고추 선발 및 저항성기작 연구)

  • 한상찬;김용균
    • Korean journal of applied entomology
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    • v.36 no.2
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    • pp.185-191
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    • 1997
  • One hundred seventy five red pepper varieties were bioassayed for selecting resistant varieties to northern rootknot nematode, Meloidogyne hapla. Fifteen native varieties (IT 102794, 104806, 105516, etc) and two imported varieties were proved to be resistant while the currently cultivated varieties such as Hongtap, Kangsan, Hongsil, and Bookang were moderately resistant to the nematodes. Resistant varieties resulted in less nematode infection and development than did the susceptibles. Roots of the resistant strains had significantly higher esterase and peroxidase activities than did those of the susceptibles.

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Antibiotic and Heavy Metal Resistance of Coliform Bacteria Isolated from Mineral Water (약수에서 分離한 大腸菌群의 일부 중금속 및 抗生劑耐性에 관한 연구)

  • Jeong, Jee-Yeon;Zong, Moon-Shik
    • Journal of Environmental Health Sciences
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    • v.15 no.1
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    • pp.63-73
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    • 1989
  • The purposes of this study were to find out the heavy metal and antibiotic resistant coliform bacteria from mineral water and the resistant factors. For the experiment, mineral water samples were taken from A area and B area during the period from march to July, 1988. The results of the experiment were as follows 1. From mineral water, eleven resistant coliforms and one susceptible coliform were isolated. 2. All resistant isolates harbored diverse plasmids of ranged ca. 14-54kb. 3. Susceptible coliform harbored a only plasmid of ca. 2.8 kb. 4. All resistant isolates harbored common size of plasmid of ca. 14kb. 5. As a result of the transformation and agarose gel electrophoresis experiments, resistant factor was R-plasmid. In conclusion, It is suggested that heavy metal contamination of mineral water is the selective pressure for the plasmid encoding the tolerance. Heavy metal resistance, in some case, is present with antibiotic resistance. Therefore, heavy metal contamination of mineral water induces antibiotic resistant bacteria.

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Screening of Some Indigenous and Exotic Mulberry Varieties against Major Foliar Fungal and Bacterial Diseases

  • Maji M.D.;Sau H.;Das B.K.;Urs S. Raje
    • International Journal of Industrial Entomology and Biomaterials
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    • v.12 no.1
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    • pp.35-39
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    • 2006
  • Fifty-six indigenous and twenty nine exotic mulberry varieties were screened against powdery mildew, Myrothecium leaf spot, Pseudocercospora leaf spot, sooty mold and bacterial leaf spot for a period of three years under field condition. The percent disease index (PDI) was recorded during peak season of the foliar diseases. Out of eighty-five varieties studied, ten varieties were highly resistant and eight were resistant to powdery mildew; six varieties were immune and seventy-eight varieties were highly resistant to Myrothecium leaf spot; sixty varieties were highly resistant and 21 were resistant to Pseudocercospora leaf spot; forty four varieties were highly resistant to sooty mold and two varieties were immune and fifty-eight were highly resistant to bacterial leaf spot. Lowest cumulatative disease index was observed in M. multicaulis (7.28) followed by Thailand lobed (7.85) and Italian mulberry (8.06).

The Fate of Tetracycline Resistant Bacteria in Biological Wastewater Treatment Plants (생물학적 하수처리장에서 테트라싸이클린 저항 세균의 거동)

  • Kim, Sungpyo
    • Journal of Korean Society on Water Environment
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    • v.22 no.3
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    • pp.527-533
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    • 2006
  • Tetracycline is one of the mostly used antibiotics around the world. The purpose of this study was to evaluate the fate of two different types of tetracycline resistant bacteria in biological wastewater treatment plants. Tetracycline resistant enterics and heterotrophic bacteria were monitored under two different lab-scale experimental conditions. Tetracycline resistant enteric bacteria showed the lower percentages of total enteric bacteria and net specific growth rate in the monitored activated sludge system as compared to tetracycline resistant heterotrophic bacteria. Therefore, total enterics, potentially E.coli, might not be the best indicator microorganism for evaluating the antibiotic resistant bacteria in biological wastewater treatment plant.

Current Status of Antimicrobial Resistance in Korea

  • Chong, Yun-Sop
    • The Journal of the Korean Society for Microbiology
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    • v.35 no.5_6
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    • pp.337-339
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    • 2000
  • MRSA, erythromycin-resistant S. pyogenes, penicillin non-susceptible pneumococci, PPNG, ESBL-producing E. coli and K. pneumoniae, class C ${\beta}$-lactamase-producing E. coli, fluoroquinolone-resistant E. coli, aminoglycoside-resistant A. baumannii and P. aeruginosa are all prevalent in Korea, which suggest the presence of high levels of antimicrobial selective pressure and nosocomial spread of resistant bacteria. Rapid increase of VRE and emergence of fluoroquinolone-resistant gonococci and VIM-2 metallo-${\beta}$-lactamase-producing P. aeruginosa are recently observed new threats in Korea.

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