• Asian-Australasian Journal of Animal Sciences
• /
• v.21 no.10
• /
• pp.1495-1500
• /
• 2008

In an attempt to develop a probiotic formulation for poultry feed, a number of lactic acid bacteria (LAB) were isolated from chicken intestinal specimens and a series of in vitro experiments were performed to evaluate their efficacy as a potential probiotic candidate. A total of 650 LAB strains were isolated and screened for their antagonistic potential against each other. Among all the isolates only three isolates (TMU121, 094 and 457) demonstrated a wide spectrum of inhibition and were thus selected for detailed investigations. All three selected isolates were able to inhibit the growth of E. coli and Salmonella species, although to variable extent. The nature of the inhibitory substance produced by the isolates TMU121 and 094 appeared to be associated with bacteriocin, as their activity was completely lost after treatment with proteolytic enzymes, while pH neutralization and catalase enzyme had no effect on the residual activity. In contrast, isolate TMU457 was able to resist the effect of proteolytic enzymes while pH neutralization completely destroyed its activity. Attempts were made to study the acid, bile tolerance and cell surface hydrophobicity of these isolates. TMU121 showed high bile salt tolerance (0.3%) and high cell surface hydrophobicity compared to the other two strains studied, while TMU094 appeared the most pH resistant strain. Based on these results, the three selected LAB isolates were considered as potential ingredients for a chicken probiotic feed formulation and were identified to species level based on their carbohydrate fermentation pattern by using API 50CH test kits. The three strains were identified as Lactobacillus fermentum TMU121, Lactobacillus rhamnosus TMU094, and Pediococcus pentosaceous TMU457.

• Journal of the Korean Society of Propulsion Engineers
• /
• v.13 no.5
• /
• pp.1-6
• /
• 2009

A development of a composite solid propellant is carried out for an application to gas generators as an energy source of rocket system. With HTPB as a propellant binder which has 80% of particle loading ratio, a favorable rheology, and moderate curing properties at the range of $-50^{\circ}{\sim}70^{\circ}C$, AN is selected as the first kind of oxidizer having the characteristics of a low flame temperature, minimal particle residual as well as nontoxic products. AP is the second oxidant for ballistic property control. A series of experiments for the improvement of physical properties were conducted and resulted in the propellant formulation having 30% of strain rate at 8 bar of max. stress.

• Proceedings of the Korean Society for Applied Microbiology Conference
• /
• 2000.04a
• /
• pp.157-167
• /
• 2000

A thermostable chitosanase gene from the isolated strain, Bacillus sp. CK4, was cloned, and its complete DNA sequence was determined. The thermostable chitosanase gene was composed of an 822-bp open reading frame which encodes a protein of 242 amino acids and a signal peptide corresponding to a 30 kDa enzyme in size. The deduced amino acid sequence of the chitosanase from Bacillus sp. CK4 exhibits 76.6%, 15.3%, and 14.2% similarities to those from Bacillus subtilis, Bacillus ehemensis, and Bacillus circulans, respectively. C-terminal homology analysis shows that Bacillus sp. CK4 belongs to the Cluster III group with Bacillus subtilis. The size of the gene was similar to that of a mesophile, Bacillus subtilis showing a higher preference for codons ending in G or C. The functional importance of a conserved region in a novel chitosanase from Bacillus sp. CK4 was investigated. Each of the three carboxylic amino acid residues were changed to E50D/Q, E62D/Q, and D66N/E by site-directed mutagenesis. The D66N/E mutants enzymes had remarkably decreased kinetic parameters such as $V_{max}$ and k$\sub$cat/, indicating that the Asp-66 residue was essential for catalysis. The thermostable chitosanase contains three cysteine residues at position 49, 72, and 211. Titration of the Cys residues with DTNB showed that none of them were involved in disulfide bond. The C49S and C72S mutant enzymes were as stable to thermal inactivation and denaturating agents as the wild-type enzyme. However the half-life of the C211S mutant enzyme was less than 60 min at 80$^{\circ}C$, while that of the wild type enzyme was about 90 min. Moreover, the residual activity of C211S was substantially decreased by 8 M urea, and fully lost catalytic activity by 40% ethanol. These results show that the substitution of Cys with Ser at position 211 seems to affect the conformational stability of the chitosanase.

• Journal of Microbiology and Biotechnology
• /
• v.25 no.6
• /
• pp.918-929
• /
• 2015

Microbial enhanced oil recovery (MEOR) is being used more widely, and the biological contributions involved in MEOR need to be identified and quantified for the improvement of field applications. Owing to the excellent interfacial activity and the wide distribution of producing strains in oil reservoirs, lipopeptides have proved to be an essential part of the complex mechanisms in MEOR. In this study, crude lipopeptides were produced by a strain isolated from an indigenous community in an oil reservoir. It was found that crude lipopeptides can effectively reduce the IFT (interfacial tension) to 10^-1~10^-2 mN/m under high salinity without forming stable emulsions, and the wettability of natural sandstone can be enhanced (Amott index, from 0.36 to 0.48). The results of core flooding experiments indicate that an additional 5.2% of original oil in place can be recovered with a 9.5% reduction of injection pressure. After the shut-in period, the wettability of the core, the reduction of injection pressure, and the oil recovery can be improved to 0.63, 16.2% and 9.6%, respectively. In the microscopic flooding experiments, the crude oil in membrane, cluster, and throat states contribute nearly 90% in total of the additional oil recovery, and the recovery of membranestate oil was significantly enhanced by 93.3% after shut in. Based on the results in macro and pore scale, the IFT reduction and the wettability alteration are considered primary contributors to oil recovery, while the latter was more dominant after one shut-in period.

• Journal of Microbiology and Biotechnology
• /
• v.10 no.2
• /
• pp.238-243
• /
• 2000

An exoinulinase (${\beta}-D-fructofuranosidase$) gene was cloned by chromosome walking along the upstream region of the endoinulinase gene of Pseudomonas mucidolens isolated from soil. the exoinulinase gene consisted of an ORF of 0,506 bp encoding a polypeptide of 501 amino acids with a deduced molecular weight of 55,000. The exoinulinase produced by the recombinant Escherichia coli $DH5{\alpha}$ strain was also purified to homogeneity as determined by SDS-PAGE and a zymogram. The molecular weight of the purified exoinulinase according to both SDS-PAGE and gel filtration matched the deduced molecular weight of the protein described above, thereby indicating that the native form of the exoinulinase was a monomer. The purified enzyme hydrolyzed activity value of 2.0. Furthermore, no inulo-oligomers were liberated from the inulin substrate in the enzymatic reaction mixtures incubated for 90 min at $55^{\circ}C$. Taken together, these results indicate that the purified ${\beta}-D-fructofuranosidase$ was an exoinulinase. The pH and temperature optima of the exoinulinase were pH 6.0 and $55^{\circ}C$, respectively. the enzymehad no apparent requirement for a cofactor, and its activity was completely inactivated by $Ag^{+},{\;}Hg^{2+},{\;}and{\;}Zn^{2+}$. Kinetic experiments gave $K_m,{\;}V_{max},{\;}and{\;}K_{cat}$ values for inulin of 11.5 mM, 18 nM/s, and $72{\;}s^{-1}$, respectively. the exoinulinase was fairly stable in broad pH conditions (pH 5-9), and at pH 6.0 it showed a residual activity of about 70% after 4 h incubation at $55^{\circ}C$.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.6 no.3
• /
• pp.75-86
• /
• 1986

The welded joint of the rail of railroad had shown the complicated behaviour of fatigue crack growth due to the effect of low toughness, residual stress, welding stress and strain etc. resulting from welding. Also, the welding connection has been necessary as making longer the rail, thus fatique fracture has often occurred at welded joints. From above, in this paper, faigue test was done at base metal, gas pressure and thermit welded joints to give the basic data to construct S-N diagrams. From this, it was known that the base metal was better than welded joint and gas pressure better than thermit welded joints in the resistance against the behaviour of fatigue crack growth. And it was also found that it is very dangerous to control fracture only by the method of material mechanics.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.32 no.6A
• /
• pp.399-409
• /
• 2012

The flexural behavior of HSB plate girder with a non-slender web, due to inelastic lateral-torsional buckling, under uniform bending was investigated by the nonlinear finite element analysis. Both homogeneous sections fabricated from SM570-TMC, HSB600 or HSB800 steel and hybrid sections with HSB800 flanges and SM570-TMC web were considered. The flanges and web of selected noncomposite I-girders were modeled as thin shell elements and the geometrical and material nonlinear finite element analysis was performed by the ABAQUS program. The steel was assumed as an elasto-plastic strain hardening material. Initial imperfections and residual stresses were taken into account and their effects on the inelastic lateral-torsional buckling behavior were analyzed. The flexural strengths of selected sections obtained by the finite element analysis were compared with the nominal flexural strengths from KHBDC LSD, AASHTO LRFD, and Eurocode and the applicability of these codes in predicting the inelastic lateral torsional buckling strength of HSB plate girders with a non-slender web was assessed.

• Journal of Korean Society of Steel Construction
• /
• v.22 no.4
• /
• pp.377-388
• /
• 2010

The flexural behavior of composite HSB600 and HSB800 I-girders under a positive moment was investigated using the material non-linear moment-curvature analysis method. Three representative composite sections with different ductility properties were selected as the baseline sections in this study. Using these baseline sections, the moment-curvature program was verified by comparing the flexural strength and the moment-curvature curve obtained from the program with those obtained using the non-linear FE analysis of ABAQUS. In the FE analysis, the composite girders were modeled three-dimensionally with flanges, the web, and the concrete slab as thin shell elements, and initial imperfections and residual stresses were imposed on the FE model. In the moment-curvature and FE analyses, the 28-day compressive strength of the concrete slab was assumed to be 30-50 MPa, and the HSB600 and HSB800 steels were modeled as elasto-plastic strain-hardening materials, with the concrete as the CEB-FIP model. The effects of the ductility ratio of the composite girder, the type of steel, the compressive strength of the concrete deck, and the location of the plastic neutral axis on the flexural characteristics were analyzed.

• Journal of the Korea Concrete Institute
• /
• v.20 no.6
• /
• pp.785-796
• /
• 2008

Longitudinal elongation develops in reinforced concrete beams that exhibit flexural yielding during cyclic loading. The longitudinal elongation can decrease the shear strength and deformation capacity of the beams. In the present study, nonlinear truss model analysis was performed to study the elongation mechanism of reinforced concrete beams. The results showed that residual tensile plastic strain of the longitudinal reinforcing bar in the plastic hinge is the primary factor causing the member elongation, and that the shear-force transfer mechanism of diagonal concrete struts has a substantial effect on the magnitude of the elongation. Based on the analysis results, a simplified method for evaluating member elongation was developed. The proposed method was applied to test specimens with various design parameters and loading conditions.

• Journal of Ocean Engineering and Technology
• /
• v.22 no.6
• /
• pp.75-82
• /
• 2008

Flame bending has been extensively used in the shipbuilding industry for hull plate forming In flame bending it is difficult to obtain the desired shape because the residual deformation dependson the complex temperature distribution and the thermal plastic strain. Mechanical bending such as reconfigurable press forming multi-point press forming or die-less forming has been found to improve the automation of hull plateforming because it can more accurately control the desired shape than line heating. Multi-point forming is a process in which external forces are used to form metal work-pieces. Therefore it can be a flexible and efficient forming technique. This paper presents an optimal approach to determining the press-stroke for multi-point press forming of curved shapes. An integrated configuration of Finite element analysis (FEA) and spring-back compensation algorithm is developed to calculate the strokes of the multi-point press. Not only spring-back is modeled by elastic plastic shell elements but also an iterative algorithm to compensate the spring-back is applied to adjust the amount of pressing stroke. An iterative displacement adjustment (IDA) method is applied by integration of the FEA procedure and the spring-back compensation work. Shape deviation between the desired surface and deform￡d plate is minimized by the IDA algorithm.