• 제목/요약/키워드: Research and development

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면역조직화학염색법을 이용한 소 합포체성 폐렴바이러스의 검출 및 병리학적 소견 (The Detection of Bovine Respiratory Syncytial Virus Using Immunohistochemistry and pathologic Findings)

  • 윤순식;황의경;문운경;배유찬;김재훈;엄현종;조재진;진영화;최상호
    • 한국수의병리학회지
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    • 제1권2호
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    • pp.157-160
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    • 1997
  • Two 2 and 7 months-old holstin and one 3 months-old native Korean calf suffering from respiratory illness were submitted to the National Veterinary Research Institute for euthanasia and pathologic examination. At necropsy diffuse lobar pneumonia was present in apical cardiac and diaphragmatic lobes in all calves. Microscopically acute multifocal bronchiolitis and interstitial syncytial cell formation were frequently observed. in addition occlusion of bronchiolar lumen due to cellular proliferation and fibrosis accompanied by interlobular septal emphysema were also present. Immunohistochemically bovine respiratory syncytial virus antigen were positively identified in lung lesions including bronchial and bronchiolar epithelium alveolar macrophages and lymphocytes.

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중관촌(中關村) 클러스터 연구개발 네트워크의 특성 (The characteristics of R&D Network in Zhongguancun Cluster)

  • 첨군;이철우
    • 한국경제지리학회지
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    • 제15권4호
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    • pp.550-569
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    • 2012
  • 본 연구에서는 중국 베이징시(북경시(北京市)) 중관촌(中關村) 클러스터를 사례로 첨단산업 연구개발 네트워크의 특성을 기업간, 산 학, 산 연, 산 관에 초점을 두고 고찰하였다. 분석결과는 다음과 같다. 중관촌(中關村) 클러스터 기업의 국제경쟁력을 제고시키기 위해서는 연구개발의 강화가 요구된다. 기업의 연구개발 네트워크 실태를 조사한 결과를 살펴보면, 조사 대상 기업들은 현재에도 어느 정도는 연구개발 네트워크가 활성화되고 있다. 특히 기업의 협력 대상별 연구개발 네트워크의 내용에 있어서 차이는 있으나, 기업들은 다양한 내용으로 연구개발 네트워크를 이용하고 있다. 그러나 연구개발 네트워크가 어느 정도 자리잡은 수준에 이르고 있지만 아직까지 기업들이 연구개발 네트워크를 구축하는데 많은 애로가 있는 것이 사실이다. 따라서 본문은 기업들이 연구개발 네트워크를 형성하는데 애로점을 해결하기 위해 개선방안을 제시하였다.

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Molecular Cloning of a Cellulase Gene from Abalone Haliotis discus hannai and Its Expression in E coli

  • Park, Eun-Mi;Han, Yun-Hee;Park, In-Suk;Nam, Bo-Hye;Kong, Hee Jeong;Kim, Woo-Jin;Lee, Sang-Jun;Kim, Young-Ok
    • 한국해양바이오학회지
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    • 제2권2호
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    • pp.108-112
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    • 2007
  • A cellulase (endo-${\beta}$-1,4-D-glucanase(E.C.3.2.1.4)) was isolated from the hepatopancreas of abalone Haliotis discus hannai by EST analysis. The abalone cellulase named HdEG compassed 1977 bp, including 195 bp in the 5'untranslated region, 1680 bp in the open reading frame which encodes 560 amino acid residues, and 92 bp in the 3'-untranslated region. The C-terminal region of the HdEG showed 44-52% identity to the catalytic domains of glycoside hydrolase family 9 (GHF9)-cellulases from arthropods and bacteria. The recombinant cellulase, pEHdEG was produced in E. coli with being fused with C-terminal His-tag. The expressed protein showed a single band (~62 kDa) on Western blotting which was consistent with the value (61,878 Da) calculated from the DNA sequence.

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Gene Cloning, Expression, and Characterization of Glucose-1-Phosphatase from Enterobacter cloacae B11

  • Kim, Young-Ok;Park, In-Suk;Nam, Bo-Hye;Kong, Hee-Jeong;Kim, Woo-Jin;Lee, Sang-Jun;Kim, Kyung-Kil
    • Fisheries and Aquatic Sciences
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    • 제13권1호
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    • pp.49-55
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    • 2010
  • A bacterial strain with phytase and glucose-1-phosphatase activity was isolated from seawater. The colony was identified as an Enterobacter cloacae strain and named E. cloacae B11. A gene, agpEnB11, coding for an intracellular acid glucose phosphatase was cloned from the strain and sequenced. It comprised 1,242 nucleotides and encoded a polypeptide of 413 amino acids. Recombinant glucose-1-phosphatase (AgpEn) was overexpressed in Escherichia coli and purified using Ni-NTA column under native conditions. Purified protein displayed a single band of 47 kDa on SDS-PAGE. AgpEn hydrolyzed a wide variety of phosphorylated compounds, with high activity for glucose-1-phosphate and glucose-6-phosphate. Optimum pH and temperature for enzyme activity were pH 5.0 and $50^{\circ}C$, respectively. Enzyme activity was stimulated by $Ca^{2+}$ and $Co^{2+}$, and inhibited by $Cu^{2+}$.

분산 독립형 변전소 종합 보호 제어 시스템 개발 (Development of Distributive Independent IDPACS(Integrated Digital Protection And Control System))

  • 고재학;조경래;안복찬;원희광;이권한;조남국;신희승
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 1996년도 하계학술대회 논문집 B
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    • pp.919-921
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    • 1996
  • Recently, a growing demands on electricity require a high performance and reliable operation in the area of protection, control and monitoring of power plants and substations which may not provides with conventional systems. This paper describes a novel IDPACS (Integrated Digital Protection And Control System) in which we integrate all protection, control and monitoring function of the substations. The simulation results show that the proposed system provides fast response and reliable operation of power plant with superb performance on protection and control system.

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유통 마른 김(Porphyra sp.)의 중금속(Hg, Pb, Cd) 함량과 위해성 평가 (Heavy Metals (Hg, Pb, Cd) Content and Risk Assessment of Commercial Dried Laver Porphyra sp.)

  • 손광태;권지영;조미라;최우석;강성림;하나영;신진월;박큰바위;김지회
    • 한국수산과학회지
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    • 제45권5호
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    • pp.454-459
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    • 2012
  • To investigate heavy metals (Hg, Pb and Cd) and their potential health risks in commercial dried laver (Porphyra sp.), we collected 45 samples from the major production areas on the western and southern coasts of Korea (Hwaseong, Seocheon, Gunsan, Muan, Shinan, Jindo, Haenam, Wando, Jangheung, Goheung and Busan). The Hg, Pb and Cd concentrations were measured using inductively coupled plasma spectrometry (ICP-MS) or a mercury analyzer. The average Hg, Pb and Cd concentrations in the dried laver were $0.006{\pm}0.0017$, $0.196{\pm}0.0614$ and $0.894{\pm}0.4882$ mg/kg, respectively. Based on the 2007 Korean Public Nutrition Report, these levels are 0.02, 0.11 and 2.47% of the provisional tolerable weekly intake (PTWI) for Hg, Pb and Cd, respectively, established by the FAO/WHO. The hazard quotient (HQ) determined from the ratio of exposure and safe levels were less than 1.0. Therefore, the levels of overall exposure to Hg, Pb and Cd for dried laver were below the recommended JECFA (Joint FAO/WHO Expert Committee on Food Additives) levels, which indicate safe levels for public health.

Flow-accelerated corrosion assessment for SA106 and SA335 pipes with elbows and welds

  • Kim, Dong-Jin;Kim, Sung-Woo;Lee, Jong Yeon;Kim, Kyung Mo;Oh, Se Beom;Lee, Gyeong Geun;Kim, Jongbeom;Hwang, Seong-Sik;Choi, Min Jae;Lim, Yun Soo;Cho, Sung Hwan;Kim, Hong Pyo
    • Nuclear Engineering and Technology
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    • 제53권9호
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    • pp.3003-3011
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    • 2021
  • A FAC (flow-accelerated corrosion) test was performed for a straight pipe composed of the SA335 Gr P22 and SA106 Gr B (SA106-SA335-SA106) types of steel with welds as a function of the flow rate in the range of 7-12 m/s at 150 ℃ and with DO < 5 ppb at pH levels ranging from 7 to 9.5 up to a cumulative test time of 7200 h using the FAC demonstration test facility. Afterward, the experimental pipe was examined destructively to investigate opposite effects as well as entrance effects. In addition, the FAC rate obtained using a pipe specimen with a 50 mm inner diameter was compared with the rate obtained from a rotating cylindrical electrode. The effects of the complicated fluid flows at the elbow and orifice of the pipeline were also evaluated using another test section designed to examine the independent effects of the orifice and the elbow depending on the distance and the combined effects on orifice and elbow. The tests were performed under the following conditions: 130-150 ℃, DO < 5 ppb, pH 7 and a flow rate of 3 m/s. The FAC rate was determined using the thickness change obtained from commercial room-temperature ultrasonic testing (UT).