• Title/Summary/Keyword: Research and development

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The Detection of Bovine Respiratory Syncytial Virus Using Immunohistochemistry and pathologic Findings (면역조직화학염색법을 이용한 소 합포체성 폐렴바이러스의 검출 및 병리학적 소견)

  • Yoon, Soon-Seek;Hwang, Eui-Kyung;Moon, Oun-Kyong;Bae, You-Chan;Kim, Jae-Hoon;Eom, Hyun-Jong;Cho, Jae-Jin;Jean, Young-Hwa;Choi, Sang-Ho
    • Korean Journal of Veterinary Pathology
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    • v.1 no.2
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    • pp.157-160
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    • 1997
  • Two 2 and 7 months-old holstin and one 3 months-old native Korean calf suffering from respiratory illness were submitted to the National Veterinary Research Institute for euthanasia and pathologic examination. At necropsy diffuse lobar pneumonia was present in apical cardiac and diaphragmatic lobes in all calves. Microscopically acute multifocal bronchiolitis and interstitial syncytial cell formation were frequently observed. in addition occlusion of bronchiolar lumen due to cellular proliferation and fibrosis accompanied by interlobular septal emphysema were also present. Immunohistochemically bovine respiratory syncytial virus antigen were positively identified in lung lesions including bronchial and bronchiolar epithelium alveolar macrophages and lymphocytes.

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The characteristics of R&D Network in Zhongguancun Cluster (중관촌(中關村) 클러스터 연구개발 네트워크의 특성)

  • Jun, Zhan;Lee, Chul Woo
    • Journal of the Economic Geographical Society of Korea
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    • v.15 no.4
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    • pp.550-569
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    • 2012
  • This paper studies the R&D network of the Zhongguancun Cluster, the most representative innovative cluster of the high-tech industry in China at present. For this study, Zhongguancun Cluster was the first high-tech cluster to have been created in China, the current Zhongguancun Cluster has played a leading role in the development of the high-tech industry in China. In addition, the Zhongguancun Cluster has at helped elevate China as a key region in terms of research development in relation to the high-tech industry. In particular, companies evaluate the research development network culture of Zhongguancun cluster positively and this increases their tendency to approach the research development network with an open mind. The main contents in research development network are as follows; (1) joint or consigned research development and technology transfer in the case of research development network between companies, (2) joint or consigned research development, human resource support and employment, technical advice and consultant and technology transfer in the case of industrial-educational relationships (3) joint or consigned research development, technical advice and consultant and technology transfer in the case of industrial-research relationships, (4) financial support and participation in joint research development project held by government in the case of industrial and governmental relationships (5) financing for research development, utilization of research human resources, purchase of research development and use of research development equipment for mediators. The main difficulties in creating a research development network are 'lack of mutual trust', 'financial problems of companies' and 'lack of information'. Especially, the main key obstacles for the most important research development network between industry and government in the case of the Zhongguancun Cluster are 'complicated and strict procedures. Therefore, more accurate and detailed understanding of the conditions and the following institutional measures from government are required for research development among companies.

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Molecular Cloning of a Cellulase Gene from Abalone Haliotis discus hannai and Its Expression in E coli

  • Park, Eun-Mi;Han, Yun-Hee;Park, In-Suk;Nam, Bo-Hye;Kong, Hee Jeong;Kim, Woo-Jin;Lee, Sang-Jun;Kim, Young-Ok
    • Journal of Marine Bioscience and Biotechnology
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    • v.2 no.2
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    • pp.108-112
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    • 2007
  • A cellulase (endo-${\beta}$-1,4-D-glucanase(E.C.3.2.1.4)) was isolated from the hepatopancreas of abalone Haliotis discus hannai by EST analysis. The abalone cellulase named HdEG compassed 1977 bp, including 195 bp in the 5'untranslated region, 1680 bp in the open reading frame which encodes 560 amino acid residues, and 92 bp in the 3'-untranslated region. The C-terminal region of the HdEG showed 44-52% identity to the catalytic domains of glycoside hydrolase family 9 (GHF9)-cellulases from arthropods and bacteria. The recombinant cellulase, pEHdEG was produced in E. coli with being fused with C-terminal His-tag. The expressed protein showed a single band (~62 kDa) on Western blotting which was consistent with the value (61,878 Da) calculated from the DNA sequence.

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Gene Cloning, Expression, and Characterization of Glucose-1-Phosphatase from Enterobacter cloacae B11

  • Kim, Young-Ok;Park, In-Suk;Nam, Bo-Hye;Kong, Hee-Jeong;Kim, Woo-Jin;Lee, Sang-Jun;Kim, Kyung-Kil
    • Fisheries and Aquatic Sciences
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    • v.13 no.1
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    • pp.49-55
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    • 2010
  • A bacterial strain with phytase and glucose-1-phosphatase activity was isolated from seawater. The colony was identified as an Enterobacter cloacae strain and named E. cloacae B11. A gene, agpEnB11, coding for an intracellular acid glucose phosphatase was cloned from the strain and sequenced. It comprised 1,242 nucleotides and encoded a polypeptide of 413 amino acids. Recombinant glucose-1-phosphatase (AgpEn) was overexpressed in Escherichia coli and purified using Ni-NTA column under native conditions. Purified protein displayed a single band of 47 kDa on SDS-PAGE. AgpEn hydrolyzed a wide variety of phosphorylated compounds, with high activity for glucose-1-phosphate and glucose-6-phosphate. Optimum pH and temperature for enzyme activity were pH 5.0 and $50^{\circ}C$, respectively. Enzyme activity was stimulated by $Ca^{2+}$ and $Co^{2+}$, and inhibited by $Cu^{2+}$.

Development of Distributive Independent IDPACS(Integrated Digital Protection And Control System) (분산 독립형 변전소 종합 보호 제어 시스템 개발)

  • Koh, Jae-Hak;Cho, Kyung-Lae;Ahn, Bog-Chan;Won, Hoi-Kwang;Lee, Kwon-Han;Cho, Nam-Gook;Shin, Hee-Seung
    • Proceedings of the KIEE Conference
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    • 1996.07b
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    • pp.919-921
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    • 1996
  • Recently, a growing demands on electricity require a high performance and reliable operation in the area of protection, control and monitoring of power plants and substations which may not provides with conventional systems. This paper describes a novel IDPACS (Integrated Digital Protection And Control System) in which we integrate all protection, control and monitoring function of the substations. The simulation results show that the proposed system provides fast response and reliable operation of power plant with superb performance on protection and control system.

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Heavy Metals (Hg, Pb, Cd) Content and Risk Assessment of Commercial Dried Laver Porphyra sp. (유통 마른 김(Porphyra sp.)의 중금속(Hg, Pb, Cd) 함량과 위해성 평가)

  • Son, Kwang-Tae;Kwon, Ji-Young;Jo, Mi-Ra;Choi, Woo-Seok;Kang, Sung-Rim;Ha, Na-Young;Shin, Jin-Wall;Park, Kunbawui;Kim, Ji-Hoe
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.45 no.5
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    • pp.454-459
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    • 2012
  • To investigate heavy metals (Hg, Pb and Cd) and their potential health risks in commercial dried laver (Porphyra sp.), we collected 45 samples from the major production areas on the western and southern coasts of Korea (Hwaseong, Seocheon, Gunsan, Muan, Shinan, Jindo, Haenam, Wando, Jangheung, Goheung and Busan). The Hg, Pb and Cd concentrations were measured using inductively coupled plasma spectrometry (ICP-MS) or a mercury analyzer. The average Hg, Pb and Cd concentrations in the dried laver were $0.006{\pm}0.0017$, $0.196{\pm}0.0614$ and $0.894{\pm}0.4882$ mg/kg, respectively. Based on the 2007 Korean Public Nutrition Report, these levels are 0.02, 0.11 and 2.47% of the provisional tolerable weekly intake (PTWI) for Hg, Pb and Cd, respectively, established by the FAO/WHO. The hazard quotient (HQ) determined from the ratio of exposure and safe levels were less than 1.0. Therefore, the levels of overall exposure to Hg, Pb and Cd for dried laver were below the recommended JECFA (Joint FAO/WHO Expert Committee on Food Additives) levels, which indicate safe levels for public health.

Flow-accelerated corrosion assessment for SA106 and SA335 pipes with elbows and welds

  • Kim, Dong-Jin;Kim, Sung-Woo;Lee, Jong Yeon;Kim, Kyung Mo;Oh, Se Beom;Lee, Gyeong Geun;Kim, Jongbeom;Hwang, Seong-Sik;Choi, Min Jae;Lim, Yun Soo;Cho, Sung Hwan;Kim, Hong Pyo
    • Nuclear Engineering and Technology
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    • v.53 no.9
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    • pp.3003-3011
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    • 2021
  • A FAC (flow-accelerated corrosion) test was performed for a straight pipe composed of the SA335 Gr P22 and SA106 Gr B (SA106-SA335-SA106) types of steel with welds as a function of the flow rate in the range of 7-12 m/s at 150 ℃ and with DO < 5 ppb at pH levels ranging from 7 to 9.5 up to a cumulative test time of 7200 h using the FAC demonstration test facility. Afterward, the experimental pipe was examined destructively to investigate opposite effects as well as entrance effects. In addition, the FAC rate obtained using a pipe specimen with a 50 mm inner diameter was compared with the rate obtained from a rotating cylindrical electrode. The effects of the complicated fluid flows at the elbow and orifice of the pipeline were also evaluated using another test section designed to examine the independent effects of the orifice and the elbow depending on the distance and the combined effects on orifice and elbow. The tests were performed under the following conditions: 130-150 ℃, DO < 5 ppb, pH 7 and a flow rate of 3 m/s. The FAC rate was determined using the thickness change obtained from commercial room-temperature ultrasonic testing (UT).

Expressed sequence tags analysis of immune-relevant genes in rock bream Oplegnathus fasciatus peripheral leukocytes stimulated with LPS

  • Lee, Jeong-Ho;Noh, Jae-Koo;Kim, Hyun-Chul;Park, Choul-Ji;Min, Byung-Hwa;Choi, Sang-Jun;Myeong, Jeong-In;Park, Hyung-Jun;Park, Chan-Il
    • Journal of fish pathology
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    • v.22 no.3
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    • pp.353-366
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    • 2009
  • We constructed a rock bream Oplegnathus fasciatus leukocyte cDNA library and a total of 795 expressed sequence tag (EST) clones were generated. Gene annotation procedures and homology searches of the sequenced ESTs were locally done by BLASTX for amino acid similarity comparisons. Of the 795 EST clones, 491 different ESTs showed significant homology to previously described genes while 304 ESTs were unidentified, hypothetical, or unnamed proteins. Encoding 121 different sequences were identified as putative bio-defense genes or genes associated with immune response.