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Anti-inflammatory Activities Verification of Vaccinum oldhami Fruit Ethanol Extracts on RAW 264.7 (RAW 264.7을 이용한 정금나무 열매(Vaccinum oldhami fruit)의 항염증 효과)

  • Lee, Jin-Young;Joo, Da-Hye;Yoo, Dan-Hee;Chae, Jung-Woo
    • Journal of Life Science
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    • v.27 no.4
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    • pp.417-422
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    • 2017
  • The purpose of this study was to investigate the role of the Vaccinum oldhami fruit extract as a cosmetic additive. As a result of having macrophage (RAW 264.7) measured a cell toxicity effects of 70% ethanol extract from Vaccinum oldhami fruit, it shown 118% with toxicity at $500{\mu}g/ml$ concentration. In nitric oxide synthesis inhibition effect, 70% ethanol extracts from Vaccinum oldhami fruit shown 47.3% at $1,000{\mu}g/ml$ concentration. The iNOS, COX-2 protein expression inhibitory effect by western blot of 70% ethanol extract from Vaccinum oldhami fruit was decreased by 36.13%, 29.61% at $500{\mu}g/ml$ concentration. And iNOS, COX-2 mRNA expression inhibitory effect by reverse-transcription-PCR of 70% ethanol extract from Vaccinum oldhami fruit was decreased by 62.25%, 90.07% at $500{\mu}g/ml$ concentration. All these finding that extract from Vaccinum oldhami fruit could prove that their have effects anti-inflammatory efficacy. And extract from Vaccinum oldhami fruit has potential as a cosmetic ingredients.

일산 이산포 역사시대의 미고생물학적 연구

  • Lee, Sang-Heon;Kim, Ju-Yong;Yang, Dong-Yun;Hong, Se-Seon;Sin, Suk-Jeong
    • Proceedings of the Korean Quaternary Association Conference
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    • 2004.11a
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    • pp.75-76
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    • 2004
  • 일산 이산포 BH-4 발굴단면의 퇴적물 시료로부터 화분 포자 및 담수성과 해양성조류화석이 다양하고 풍부하게 산출되었다. 산출된 식물기원 미화석 중, 목본화분 $25{\sim}60%$, 초본화분 $15{\sim}70%$ 그리고 포자 $4{\sim}30%$ 각각 구성 되었다. 또한 담수성 조류(freshwater algae)는 전체 단면구간에서 산출된 반면, 와편모류(dinoflagellate), 아크리타치(acritarch)와 같은 해양성조류(marine algae)는 하부단면구간(절대고도: 3.31-2.74 m)에서만 산출되었다. 산출된 화분 포자 및 통계처리기법(CONISS)을 이용하여 2개의 지역화분대 (local pollen a assemblage zone) 및 4개의 아화분대(local pollen assemblage subzone)가 설정되었다. 제I화분대(시료 $Al-2{\sim}A6-1$, 고도 $2.70{\sim}3.31\;m$)는 다시 제Ia아화분대(시료 $Al-2{\sim}A3-1$, 고도 $2.70{\sim}3.00\;m$)와 제Ib아화분대(시료 $A4-2{\sim}A6-1$, 고도 $3.01{\sim}3.31\;m$)로 세분되었으며; 제II화분대(시료 $A7-2{\sim}A10-1$, 고도 $3.32{\sim}3.72\;m$) 역시 두개의 아화분대인 제IIa(시료 $A7-2{\sim}A8-1$, 고도 $3.32{\sim}3.51\;m$)와 제lIb(시료 $A9-2{\sim}A10-1$, 고도 $3.53{\sim}3.72\;m$)로 이루어졌다. 화분군집조성에 의하면, 미화석들이 퇴적되었던 시기동안에 2개의 산림(forest)형태가 발달했던 나타났다. 제I화분대(고도, $3.31{\sim}2.74\;m$)의 시기동안에는 침엽수와 낙엽활엽수로 이루어진 혼합림으로, 주요 식생은 2엽소나 무(Diploxylon), 가문비((Picea), 낙엽송(Larix)의 침엽수와 참나무(Quercus), 자작나무(Betula), 개암나무(Corylus), 중국굴피 나무속(Pterocarya)의 낙엽활엽수 등이 산악-구릉지에서 주로 서식하였던 것으로 나타났다. 또한 습생나무인 오리나무(Alnus), 버드나무(Salix)등과 해안가 등지에서 잘 서식하는 양치식물과 사초과(Cyperaceae), 명아주과(Chenopodiaceae) 같은 풀들이 하구언(estuary)의 해안가에 주로 서식하였던 것으로 나타났다. 이 시기동안에 해양성조류인 와편모류와 담수성 조류가 동시에 다양하게 산출되는 것은 하구언 퇴적환경이었음을 잘 지시해 준다. 제II화분대(고도, 43.72{\sim}3.36\;m$)가 시작되면서 혼합림은 침엽수림으로 바뀌었으며, 낙엽활엽수들의 서식지가 2엽소나무로 대치되면서 침엽수가 주요 수종을 이루었던 것으로 나타났다. 아마도 이런 산림형태는 자연 기후환경변화 뿐만 아니라 인간간섭에 의해 나타난 결과로 생각된다. 이 시기에 경작식물인 벼과(Gramineae), 옥수수(Zea), 메밀(Fagopyrum)과 논밭의 뚝 등지 에서 서식하는 돼지풀(Ambrosia) 등이 처음으로 산출되는 것으로 보아 인간간섭이 행해졌음을 잘 뒷 받침해준다. 또한 담수성 조류는 변함없이 산출되는 반면, 해양성조류인 와편모류가 산출되지 않는 것으로 보아 해안환경에서 다소 멀 어진 저지대의 습윤한 충적대지환경으로 변하였던 것으로 판단된다.

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Characteristics of Goroshoe (Acer mono Max.) Sap with Different Collection Times After Ultra Filtration (한외여과 처리에 따른 채취시기별 고로쇠 수액의 품질특성)

  • Jeong, Su-Jeong;Lee, Chang-Hyeon;Kim, Hyun-Young;Hwang, In-Guk;Shin, Chang-Seob;Park, Eui-Seok;Lee, Jun-Soo;Jeong, Heon-Sang
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.5
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    • pp.753-758
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    • 2011
  • We investigated the effects of ultra filtration (UF) of Goroshoe (Acer mono Max.) sap collected in March and April. The total bacteria and turbidity of the sap collected in April were higher than in March. However, after UF, the turbidity decreased and bacteria were not detected. The total acidity and total sugar of the sap collected in April were lower than that collected in March. After UF, total acidity and total sugar decreased slightly in sap from both of the collection times. The crude ash and mineral content of the sap collected in April were higher than in March. In particular, the calcium and potassium content of the sap collected in April were 166.38 and 29.47 mg/L, respectively, which was much higher than in March. Again, after UF, the crude ash and mineral content were decreased in the sap from both collection times. We concluded that UF of Goroshoe sap increased its quality regardless of collection time.

Effects of cultivation ages and modes on microbial diversity in the rhizosphere soil of Panax ginseng

  • Xiao, Chunping;Yang, Limin;Zhang, Lianxue;Liu, Cuijing;Han, Mei
    • Journal of Ginseng Research
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    • v.40 no.1
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    • pp.28-37
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    • 2016
  • Background: Panax ginseng cannot be cultivated on the same land consecutively for an extended period, and the underlying mechanism regarding microorganisms is still being explored. Methods: Polymerase chain reaction and denaturing gradient gel electrophoresis (PCR-DGGE) and BIO-LOG methods were used to evaluate the microbial genetic and functional diversity associated with the P. ginseng rhizosphere soil in various cultivation ages and modes. Results: The analysis of microbial diversity using PCR-DGGE showed that microbial communities were significantly variable in composition, of which six bacterial phyla and seven fungal classes were detected in P. ginseng soil. Among them, Proteobacteria and Hypocreales dominated. Fusarium oxysporum, a soilborne pathogen, was found in all P. ginseng soil samples except R0. The results from functional diversity suggested that the microbial metabolic diversity of fallow soil abandoned in 2003was the maximum and transplanted soil was higher than direct-seeding soil and the forest soil uncultivated P. ginseng, whereas the increase in cultivation ages in the same mode led to decreases in microbial diversity in P. ginseng soil. Carbohydrates, amino acids, and polymers were the main carbon sources utilized. Furthermore, the microbial diversity index and multivariate comparisons indicated that the augmentation of P. ginseng cultivation ages resulted in decreased bacterial diversity and increased fungal diversity, whereas microbial diversity was improved strikingly in transplanted soil and fallow soil abandoned for at least one decade. Conclusion: The key factors for discontinuous P. ginseng cultivation were the lack of balance in rhizosphere microbial communities and the outbreak of soilborne diseases caused by the accumulation of its root exudates.

Improving of land-cover map using IKONOS image data (IKONOS 영상자료를 이용한 토지피복도 개선)

  • 장동호;김만규
    • Spatial Information Research
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    • v.11 no.2
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    • pp.101-117
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    • 2003
  • High resolution satellite image analysis has been recognized as an effective technique for monitoring local land-cover and atmospheric changes. In this study, a new high resolution map for land-cover was generated using both high-resolution IKONOS image and conventional land-use mapping. Fuzzy classification method was applied to classify land-cover, with minimum operator used as a tool for joint membership functions. In separateness analysis, the values were not great for all bands due to discrepancies in spectral reflectance by seasonal variation. The land-cover map generated in this study revealed that conifer forests and farm land in the ground and tidal flat and beach in the ocean were highly changeable. The kappa coefficient was 0.94% and the overall accuracy of classification was 95.0%, thus suggesting a overall high classification accuracy. Accuracy of classification in each class was generally over 90%, whereas low classification accuracy was obtained for classes of mixed forest, river and reservoir. This may be a result of the changes in classification, e.g. reclassification of paddy field as water area after water storage or mixed use of several classification class due to similar spectral patterns. Seasonal factors should be considered to achieve higher accuracy in classification class. In conclusion, firstly, IKONOS image are used to generated a new improved high resolution land-cover map. Secondly, IKONOS image could serve as useful complementary data for decision making when combined with GIS spatial data to produce land-use map.

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Characteristics Distribution of Vascular Plants of the Moaksan Provincial Park (모악산 도립공원일대의 관속식물 분포 특성)

  • Beon, Mu-Sup;Oh, Hyun-Kyung
    • Korean Journal of Environment and Ecology
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    • v.21 no.1
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    • pp.38-46
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    • 2007
  • The vascular plants of the Moaksan provincial park area were listed as 615 taxa: 120 families, 383 genera, 542 species, 1 subspecies, 69 varieties and 3 forms. Based on the list of Korean endemic plants, 6 taxa were recorded: Cephalotaxus koreana, Carex okamotoi, Lilium amabile, Salix purpurea var. japonica, Weigela subsessilis, Aster koraiensis and based on the list of the rare plants by the Forest Research Institute, 5 taxa were recorded in the studied areas: Lilium distichum (Preservation priority order; No. 159), Tricyrtis dilatata (No. 97), Iris ensata var. spontanea (No. 197), Aristolochia contorta (No.151), Viola albida (No. 202). Specific plant species by floral region were a total of 48 taxa; Prunus yedoensis in Class V, 2 taxa, as Carex arenicola, Wistaria floribunda in Class IV, 4 taxa, Spiraea salicifolia, Thea sinensis, Asperula lasiantha, etc. in Class III, 8 taxa, as Lilium distichum, Iris ensata var. spontanea, Acer triflorum, etc. in Class II, 33 taxa, as Camptosorus sibiricus, Orixa japonica, Lonicera praeflorens, etc. in Class I. The naturalized plants in this site were 16 families, 40 genera, 48 species, 2 varieties, 50 taxa and the naturalization rate was 8.1% of all 615 taxa vascular plants. As a conservation measure, there needs to be controls on the imprudent development and excessive visitors and a detailed plan like nature the rest of the year.

Diets and Foraging Tactics of Eurasian Eagle Owls(Bubo bubo) in Two Different Habitat Types (서로 다른 환경에서 서식하는 수리부엉이(Bubo bubo)의 먹이 이용)

  • Nam, Hyun-Young;Lee, Woo-Shin;Choi, Chang-Yong
    • Korean Journal of Environment and Ecology
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    • v.21 no.1
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    • pp.30-37
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    • 2007
  • Pellets and prey remains were analyzed to compare diets and foraging tactics of Eurasian eagle owls (Bubo bubo) in two different habitat types: forested areas and open fields. Overall 150 prey items of three taxa were identified from 66 pellets and 82 prey remains, and the birds were the most important prey in biomass (78.04%) and in frequency (56.67%). Eurasian eagle owls frequently used rats (Rattus spp.), ring-necked pheasants (Phasianus colchicus), and stripped field mice (Apodemus agrarius), but the ring-necked pheasant was most important in biomass in both habitat types. The owls generally foraged various prey in biomass but the mean mass of vertebrate prey used by the Eurasian eagle owls was 503.3g in central Korea. According to the comparison of diets in the two different habitat types, the owls used bigger and more diverse prey in forested areas than in open fields. In forested areas, the Eurasian eagle owls frequently foraged the pheasants and Mandarin ducks (Aix galericulata), but they preferred prey of particular sizes to prey of particular taxa. In open fields, however, the owls showed opportunistic foraging tactics by selecting many small mammals such as rodents or a few large birds.

Landscape Fragmentation of Circular Greenspace in Cheongju and Requirements for a Sustainable Development (청주시 환상녹지의 경관 파편화 실태와 지속가능한 녹지관리 방안 모색)

  • Kim, Jai-Han
    • Journal of the Korean Geographical Society
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    • v.47 no.1
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    • pp.79-97
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    • 2012
  • This study examines on the impact of biodiversity in circular greenspace of Cheongju city since 2002 greenbelt release. Research has carried out to investigate the biotope pattern change with landscape fragmentation. Major landscape fragmentation has occurred with development of residential sector and build-up of major highways. Settlement has been expanded to the entire area connected to urban district excluding the eastern forest. North-south district shows high road density, where inter-regional roads meet in the cross-section. It is found that landscape fragmentation impact on species richness as well as population size of the species varies depending on the animal species. The birds show high species richness in N2, N3, N4, S2(north-south zone) even with high fragmentation rate. This can be explained that birds can access to aquatic environment where they can find abundant food resources. The amphibians and the reptiles show almost no zonal variation in species richness than the birds. The more a zone fragmented in small patches, the species richness of the amphibians and the reptiles also tends to be declined. Information accumulation on biodiversity for integrating landscape planning in urban planning, various level of community participation in decision making process, and cross border cooperation with neighbouring Cheongwon-gun will be required for sustainable greenspace management of Cheongju City.

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A Study on the Planting and Use of Landscaping Plants in Chuncheon (춘천시의 조경식물의 식재와 이용 현황)

  • Jeong Jin-Hyung;Noh Hee-Sun;Lee Ki-Eui
    • Journal of the Korean Institute of Landscape Architecture
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    • v.32 no.4
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    • pp.7-20
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    • 2004
  • This study was executed to find out how to improve the planting and use of landscaping plants in Chuncheon. The number of street trees was 17,877 in 2003. The major species of street trees were Ginkgo biloba(52.7%), Prunus sargentii(15.3%), Zelkova serrata(8.9%), Platanus occidentalis(7.3%). Salix babylonica and Platanus occidentalis were decreased, while Prunus sargentii and Zelkova serrata were increased. Salix babylonica is a good species for a lakeside city, but its seeds cause allergies to some people. Most of the trees have been removed in Chuncheon. The planting of Salix babylonica(♂) resulted in no problems, and it should be propagated to plant. The soil of several areas was analysed to investigate soil conditions. The outskirts of city had good conditions in pH, organic matter and minerals, but the downtown areas had high alkalic, due to alkali from footpath concrete blocks. Appropriate pruning adds to the aesthetic and prolongs the useful life; it also requires less managing of insects and diseases to maintain good healthy of street trees. Street trees were not properly pruned due to electric lines and shortage of pruning information. The pruning was controlled by Korea Electric Power Co, which has no pruning information. Pruning must be maintained by a professional landscape company to maintain good shape such as with Bonsai. The Shrubs planting zone between street trees and other trees, and preservation plates were established for healthy of street trees. They have to be repaired and maintained well to keep better environmental conditions. The number of tree and flower species of 68 schools in Chuncheon were 12 and 16, respectively. The species that showed high preference were Juniperus chinensis, Pinus koraiensis, Pinus densiflora, Rosa centifolia, Forsythia koreana, and Rhododendron schlippenbachii. The high frequency of the protection trees designated by Chuncheon were Pinus densiflora, Ginko biloba, Zelkova serrata, Quercus species. It was thought that the diversification of street tree species, the selection of street trees suitable to each space, the generalization of use of native species, the appropriate pruning and proper fertilization, the control of pests and diseases, and the opinions of citizens concerning landscape plants were needed to improve the planting, use and maintenance of landscaping plants in Chuncheon.

A study on vegetation and soil environmental characteristics of green roof in Daejeon Metropolitan City (대전광역시 옥상녹화 지역의 식생현황 및 토양환경 특성에 관한 연구)

  • Lee, Sang-Jin;Park, Gwan-Soo;Lee, Dong-Kun;Jang, Seong-Wan;Park, Beom-Hwan;Lee, Hang-Goo;Yun, Joon-Young;Jang, Kwan-Woo;Lee, Seung-Woo;Lee, Ho-Young;Kwon, Oh-Jung;Lee, Sook-Mee;Kil, Sung-Ho
    • Korean Journal of Agricultural Science
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    • v.38 no.4
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    • pp.641-649
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    • 2011
  • This study was to analyze the soil environmental characteristics and vegetation status of green roof in Daejeon Metropolitan City. The investigated floras of vascular plants are 17 families, 26 genera, 28 species in Seo-Gu Daejeon District Office Building (SG), 25 families, 49 genera, 56 species in Galma Public Library (GP), and 34 families, 57 genera, 60 species in Daejeon City Hall (DC) respectively. Although the larger area shows the more numbers of species in introduced plants and naturalized plant, the naturalized plant ratios were similar with each other. They were 10.71%, 10.71%, and 11.67% at SG, GP, and DC respectively. As a result of analysis on soil physical property, soil depths including vegetation soil and drainage soil of 3 green roofs were 30cm. The depths of vegetation soil at SG, GP, and DC were 0~8cm, 0~10cm, 0~10cm respectively. As a results of soil chemical properties of our study, soil pH of vegetation soil and drainage soil were a range of 6.42 and 7.43, and a range of 6.55 and 7.43 on the average respectively. Available-P contents of vegetation soil and drainage soil were a range of 153.33 and 366.33mg/kg, and a range of 136.67 and 242.67 mg/kg which is very high, respectively. Carbon contents in soil at vegetation soil and drainage soil were a range of 3.16 and 6.38%, and a range of 1.63 and 2.47% respectively. Carbon storage per square meter within 30 cm were 2.76 kg, 2.99 kg, and 3.66 kg at SG, GP, and DC respectively.