• 제목/요약/키워드: Research Diversity

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Isolation and inheritance of microsatellite loci for the oily bittering (Acheilognathus koreensis): applications for analysis of genetic diversity of wild populations

  • Kim, Woo-Jin;Kong, Hee-Jeong;Shin, Eun-Ha;Kim, Chi-Hong;Kim, Hyung-Soo;Kim, Young-Ok;Nam, Bo-Hye;Kim, Bong-Seok;Lee, Sang-Jun;Jung, Hyung-Taek
    • Animal cells and systems
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    • 제16권4호
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    • pp.321-328
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    • 2012
  • The oily bittering Acheilognathus koreensis is a freshwater species that is endemic to Korea and is experiencing severe declines in natural populations as a result of habitat fragmentation and water pollution. For the conservation and restoration of this species, it is necessary to assess its genetic diversity at the population level. We developed 13 polymorphic microsatellite loci that were used to analyze the genetic diversity of two populations collected from the Kum River and the Tamjin River in Korea. All loci exhibited Mendelian inheritance patterns when examined in controlled crosses. Both populations revealed high levels of variability, with the number of alleles ranging from 3 to 20 and observed and expected heterozygosities ranging from 0.500 to 0.969 and from 0.529 to 0.938, respectively. None of the loci showed significant deviation from Hardy-Weinberg equilibrium, and one pair of loci showed significant linkage disequilibrium after Bonferroni correction. Pairwise $F_{ST}$ and genetic distance estimation showed significant differences between two populations. These results suggest that the microsatellites developed herein can be used to study the genetic diversity, population structure and conservation measure of A. koreensis.

팀 다양성과 과업난이도가 팀 창의성에 미치는 영향 : 다중 에이전트 시뮬레이션 접근방법을 중심으로 (Investigating the Effect of Both Team Diversity and Task Difficulty on Team Creativity : Multi-Agent Simulation Approach)

  • 채성욱;서영욱;이건창
    • 경영과학
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    • 제32권2호
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    • pp.111-124
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    • 2015
  • In the management literature, it has been widely accepted among both researchers and practitioners that the level of team creativity is significantly related to the organizational performance. Besides, researchers posited with confidence that team diversity and task difficulty would affect team creativity through team members' activities of exploration and exploitation. However, empirical approaches to proving this belief suffered from lack of real data and proper methods as well. To tackle the research void like this, we propose a multi-agent simulation (MAS) mechanism. By adopting a set of parameters which validity were proven in the related literature, we conducted a series of experiments in the environment of the MAS platform named NetLogo. There sults suggest that managers can differentiate team diversity strategies according to task difficulty. In the case of a difficult task, managers need to increase team diversity so that their teams can maximize team creativity through rigorous exploration and exploitation. It is desirable to maintain an average level of team diversity when performing an easy task.

생명현상의 다양성과 특징에 관한 고찰 - 보건의료에 미치는 영향을 중심으로 - (Diversity and its Characteristics of Life Phenomenon)

  • 이선동
    • 대한예방한의학회지
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    • 제14권1호
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    • pp.1-12
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    • 2010
  • This research was intended to delve into the diversity of life phenomenon and its characteristics. First of all, this research gave real examples to compare the differences in men's health, disease, and longevity in order to confirm the existence of diversity of life phenomenon. In addition, it also studied the process and mechanism of manifestation of life phenomenon, as well as the influence and problems of existing studies' results and implications. The results are as follow. 1. Differences in health, diseases, and longevity were very big and diverse in researches on different races, nations, ages, socioeconomic status, positions, and even (monozygotic) twins. 2. The basic foundation of all organisms is DNA, and environmental factors change DNA methylation and the structure of chromatin by constantly influencing DNA. Due to this, the manifestation, control, and phenotype of DNA change, resulting in diversified life phenomenon. Therefore, it is the environmental factors, not DNA, that has more influence on the diversity of life. 3. Looking at available studies, the most reasonable perspective on human requires focusing on the diversity of life phenomenon, holistic thinking, and reversible change instead of irreversible determinism. Considerable differences in life phenomenon between entities require a change in malformed perspective on life. Public health and medicine deals with live human beings, a more precise and accurate perspective on life is very important. Because management methods of health and disease, such as structure and approaches of medical research, prevention and cure, must be different by life perspectives.

ISSR 표지에 의한 천마의 유전 다양성분석 및 기능성 물질분석 (Genetic Diversity and Metabolite Analysis of Gastrodia elata by Inter-Simple Sequence Repeats (ISSR) Markers)

  • 김현태;김지아;박응준
    • 한국약용작물학회지
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    • 제20권6호
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    • pp.440-446
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    • 2012
  • Gastrodia elata, an achlorophyllous orchid plant, is rare medicinal plant. We investigated the genetic diversity in G. elata from 4 locations by using Inter-Simple Sequence Repeats (ISSR) markers. Shannon's information Index (S.I.) indicating genetic diversity ranged from 0.255 (Pocheon) to 0.322 (Muju) with the mean of 0.29. The level of genetic diversity was lower than other plant and most genetic diversity was allocated among individuals within populations (26.81%). The UPGMA dendrogram based on genetic distance failed in showing decisive geographic relationship. In the case of gastrodin (GA), the major components in G. elata, Sangju was highest. The ergothionine (ERG) was detected a lot of contents in Muju and Pocheon. In conclusion, our results is very important information for explaining relationship of genetic variation and functional substances without the effects of environment factors and developing genetic marker by ISSR in G. elata, which may be responsible for the development of breeds with a lot of functional substance in G. elata.

가정생활교육에 기반한 지속가능한 인성, 인권, 문화다양성 통합교육: 대학생을 중심으로 (Integrated Education for Sustainable Character, Human Rights, Cultural Diversity Based on Family Life Education: Focusing on University Students)

  • 김우정;주영애
    • Human Ecology Research
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    • 제58권1호
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    • pp.31-41
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    • 2020
  • This study examined human rights perception, cultural diversity perception, level of character perception, and influencing factors for university students in order to prepare basic data on integrated education methods of character, human rights and cultural diversity and education program models. The research questions are as follows. 1. What are university students' perceptions of character, cultural diversity and human rights? 2. What is the status of the experience of family life education on character, cultural diversity, and human rights, and is it related to the level of perception? 3. What variables affect character perception, cultural diversity perception, and human rights perception? The survey targeted 407 students at two universities in Seoul and used IBM PASW Statistics as a tool to analyze data. The study results are as follows. First, university students' character, cultural diversity and human rights perception were above average; however, empirical education is still required because they had low scores in the area of action. Second, male university students have less experience in family life education on character, cultural diversity and human rights compared to female university students. Third, it is necessary to integrate education for character, cultural diversity and human rights because the most influential factors in character perception, cultural diversity perception and human rights perception were character education. The role of family life education should be considered important because personality education in the home showed a relatively high correlation.

Effects of Market Diversity on Performance of Exporting Companies: An Inverted U-shaped Relationship

  • Lee, Jungeun;Kim, Chang-Bong;Lee, Dong-Jun
    • Journal of Korea Trade
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    • 제24권8호
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    • pp.121-132
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    • 2020
  • Purpose - The principle aim of this study is to further investigate the relationship between market diversity and export performance. We examine the benefits and costs of geographic market diversity regarding the number of countries exported to by firms on their export performance. Based on the financial risk reduction model and the entry costs model, we propose a way to incorporate the costs and benefits aspects of market diversity. Design/methodology - To empirically investigate our research question, the curvilinear relationship between market diversity and export performance, we built a secondary panel data set between 2015 and 2019, containing 17,863 observations of Korean exporting companies. A generalized least squares panel estimator with fixed effects was employed to test the hypothesis, and the statistical package, Stata 14, was used. Findings - Our main findings are as follows: As market diversity increases, export performance increases because exporters can diversify and reduce financial risks in export markets. However, the relationship between the two does not grow. As it peaks, the entry costs increase due to the high market diversity, thereby outweighing the benefits, leading, eventually to decrease in the export performance. Consequently, there is an inverted U-shaped relationship between market diversity and export performance. Originality/value - In the export and trade literature, the impact of market diversity on export performance has not been addressed yet, despite the importance of this subject. Many scholars have assumed a positive linear relationship between the two, considering only the decrease in market risks as the number of overseas markets increases, without examining the increase in the entry and management costs. Therefore, our study contributes by providing a new perspective for analyzing the characteristics and outcomes of market diversity.

강원도 자연환경연구공원의 육상 포유류 종다양성 (Species Diversity of Terrestial Mammalia in the Nature Environment Research Park in Gangwon Province)

  • 이기영;김의경;조중현;박영철
    • 한국환경생태학회지
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    • 제25권2호
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    • pp.145-155
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    • 2011
  • 본 연구는 2004년부터 2008년에 걸쳐 강원도 자연환경연구공원 내 산림 및 주변 산림지역을 대상(조사구역 I: 호수 및 주변 수변생태계, 조사구역II: 인가 및 주변 산림생태계, 조사구역Ⅲ: 산림생태계)으로 육상 포유동물의 종다양성과 분포현황 및 변화양상을 조사한 것이다. 본 연구 동안 멸종위기종인 하늘다람쥐, 수달, 삵 등이 연구지역 내에 서식하고 있는 것을 확인하였다. 포유동물의 종다양성은 인가 접경 산림인 조사구역II보다는 수변지역인 조사구역I과 산림지역인 조사구역III에서 다양도가 더 높은 경향을 보였다. 종다양성의 변화는 2006년에 다소 증가했으나 2007년부터 미세하게 감소하기 시작했다. 그러나 종수의 미세한 감소와는 달리 개체수는 2007년부터 매우 크게 감소하는 경향을 보였다. 따라서 연구지역 내 산림에서 포유류의 종다양성과 개체수를 일정하게 유지하기 위한 방안을 마련할 필요가 있다.

Genome-Wide SSR 마커를 이용한 주요 산지별 참당귀의 유전다양성 분석 (Genetic Diversity of Angelica gigas Nakai Collected in Korea using Genome-Wide SSR Markers)

  • 정대희;박윤미;김기윤;박홍우;전권석;김만조;길진수;이이;엄유리
    • 한국약용작물학회지
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    • 제27권6호
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    • pp.376-382
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    • 2019
  • Background: Angelica gigas Nakai has been used as an herbal medicine in Eastern Asia for treating disorders in women for a long time. To date there are no studies on the genetic diversity of A. gigas. The present study aimed to study the genetic diversity of A. gigas variants using genome-wide simple sequence repeat (SSR) markers. Methods and Results: The genetic diversity of 199 variants of A. gigas cultivated in of different regions, was analyzed using 5 genome-wide SSR markers. The results revealed that the genetic variants were very diverse, and genetic analysis using the 5 SSR markers revealed high diversity among the variants. Conclusions: It is expected that the development of the true Angleical cultivar, by studying the system and group selection, can be achieved by genetic analysis using the developed markers, for generating a genetically fixed lineage and group selection.

도라지 수집종의 형태적 특성과 SSR마커에 의한 유연관계 분석 (Morphological Characteristics and Genetic Diversity Analysis of Platycodon grandiflorum (Jacq.) A. DC Determined Using SSR Markers)

  • 엄유리;이이;;이대영;이재원;김금숙;김창국;홍창표;김옥태
    • 한국약용작물학회지
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    • 제24권1호
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    • pp.55-61
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    • 2016
  • Background : Plant breeding requires the collection of genetically diverse genetic resources. Studies on the characteristics of Platycodon grandiflorum resources have not been carried out so far. The present study was carried out to discriminate P. grandiflorum based on morphological characteristics and genetic diversity using simple sequence repeat (SSR) markers. Methods and Results :We collected 11 P. grandiflorum cultivars: Maries II, Hakone double white, Hakone double blue, Fuji white, Fuji pink, Fuji blue, Astra white, Astra pink, Astra blue, Astra semi-double blue and Jangbaek. Analyses of the morphological characteristics of the collection were conducted for aerial parts (flower, stem and leaf) and underground parts (root). Next, the genetic diversity of all P. grandiflorum resources was analyzed using SSR markers employing the DNA fragment analysis method. We determined that the 11 P. grandiflorum cultivars analyzed could be classified by plant length, leaf number and root characteristic. Based on the genetic diversity analysis, these cultivars were classified into four distinct groups. Conclusions : These findings could be used for further research on cultivar development using molecular breeding techniques and for conservation of the genetic diversity of P. grandiflorum. Moreover, the markers could be used for genetic mapping of the plant and marker-assisted selection for crop breeding.

Genetic diversity among cultivated and wild Panax ginseng populations revealed by high-resolution microsatellite markers

  • Jang, Woojong;Jang, Yeeun;Kim, Nam-Hoon;Waminal, Nomar Espinosa;Kim, Young Chang;Lee, Jung Woo;Yang, Tae-Jin
    • Journal of Ginseng Research
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    • 제44권4호
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    • pp.637-643
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    • 2020
  • Background: Ginseng (Panax ginseng Meyer) is one of the world's most valuable medicinal plants with numerous pharmacological effects. Ginseng has been cultivated from wild mountain ginseng collections for a few hundred years. However, the genetic diversity of cultivated and wild ginseng populations is not fully understood. Methods: We developed 92 polymorphic microsatellite markers based on whole-genome sequence data. We selected five markers that represent clear allele diversity for each of their corresponding loci to elucidate genetic diversity. These markers were applied to 147 individual plants, including cultivars, breeding lines, and wild populations in Korea and neighboring countries. Results: Most of the 92 markers displayed multiple-band patterns, resulting from genome duplication, which causes confusion in interpretation of their target locus. The five high-resolution markers revealed 3 to 8 alleles from each single locus. The proportion of heterozygosity (He) ranged from 0.027 to 0.190, with an average of 0.132, which is notably lower than that of previous studies. Polymorphism information content of the markers ranged from 0.199 to 0.701, with an average of 0.454. There was no statistically significant difference in genetic diversity between cultivated and wild ginseng groups, and they showed intermingled positioning in the phylogenetic relationship. Conclusion: Ginseng has a relatively high level of genetic diversity, and cultivated and wild groups have similar levels of genetic diversity. Collectively, our data demonstrate that current breeding populations have abundant genetic diversity for breeding of elite ginseng cultivars.