• Title/Summary/Keyword: Research Culture

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Improving Mycoplasma ovipneumoniae culture medium by a comparative transcriptome method

  • Wang, Xiaohui;Zhang, Wenguang;Hao, Yongqing
    • Journal of Veterinary Science
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    • v.21 no.2
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    • pp.30.1-30.11
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    • 2020
  • Mycoplasma ovipneumoniae (Mo) is difficult to culture, resulting in many difficulties in related research and application. Since nucleotide metabolism is a basic metabolism affects growth, this study conducted a "point-to-point" comparison of the corresponding growth phases between the Mo NM151 strain and the Mycoplasma mycoides subsp. capri (Mmc) PG3 strain. The results showed that the largest difference in nucleotide metabolism was found in the stationary phase. Nucleotide synthesis in PG3 was mostly de novo, while nucleotide synthesis in NM151 was primarily based on salvage synthesis. Compared with PG3, the missing reactions of NM151 referred to the synthesis of deoxythymine monophosphate. We proposed and validated a culture medium with added serine to fill this gap and prolong the stationary phase of NM151. This solved the problem of the fast death of Mo, which is significant for related research and application.

Semiotic Interpretation of Beauty Camera App Interface Design Elements: Centered on Roland Barthes' Semiotics

  • Yongfeng Liu
    • International Journal of Advanced Culture Technology
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    • v.12 no.3
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    • pp.213-224
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    • 2024
  • This study utilizes Roland Barthes' semiotic theory to explore how interface design elements of beauty camera apps, such as filters and stickers, icons and buttons, layout and structure, function as symbols of self-expression, and their impact on users' social identity and personal image. Through in-depth analysis of applications like BeautyPlus, Ulike, MeituPic, and Faceu, this research found that these apps emphasize usability, visual appeal, and social interactivity in their design. These elements not only enhance user experience and operational efficiency but also reflect popular cultural trends and aesthetic preferences. From Barthes' perspective, these interface elements have become "myths" with specific social meanings, which are naturalized in daily use and promote the widespread acceptance of particular aesthetic standards. These interface design elements are not just tools for self-expression; they are also powerful cultural symbols that shape modern social interactions and visual culture. This research offers a new perspective to understand the interactions between individuals and society in the digital age, emphasizing the role of technology, culture, and social interaction in shaping contemporary human behavior and social relationships.

Isolation of Bacteria Associated with the King Oyster Mushroom, Pleurotus eryngii

  • Lim, Yun-Jung;Ryu, Jae-San;Shi, Shanliang;Noh, Won;Kim, Eon-Mi;Le, Quy Yang;Lee, Hyun-Sook;Ro, Hyeon-Su
    • Mycobiology
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    • v.36 no.1
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    • pp.13-18
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    • 2008
  • Eight distinct bacteria were isolated form diseased mycelia of the edible mushroom, Pleurotus eryngii. 16S rDNA sequence analysis showed that the isolates belonged to a variety of bacterial genera including Bacillus (LBS5), Enterobacter (LBS1), Sphingomonas (LBS8 and LBS10), Staphylococcus (LBS3, LBS4 and LBS9) and Moraxella (LBS6). Among them, 4 bacterial isolates including LBS1, LBS4, LBS5, and LBS9 evidenced growth inhibitory activity on the mushroom mycelia. The inhibitory activity on the growth of the mushroom fruiting bodies was evaluated by the treatment of the bacterial culture broth or the heat-treated cell-free supernatant of the broth. The treatment of the culture broths or the cell-free supernatants of LBS4 or LBS9 completely inhibited the formation of the fruiting body, thereby suggesting that the inhibitory agent is a heat-stable compound. In the case of LBS5, only the bacterial cell-containing culture broth was capable of inhibiting the formation of the fruiting body, whereas the cell-free supernatant did not, which suggests that an inhibitory agent generated by LBS5 is a protein or a heat-labile chemical compound, potentially a fungal cell wall-degrading enzyme. The culture broth of LBS1 was not inhibitory. However, its cell-free supernatant was capable of inhibiting the formation of fruiting bodies. This indicates that LBS1 may produce an inhibitory heat-stable chemical compound which is readily degraded by its own secreted enzyme.

Intellectual structure and research trends of The Research Journal of the Costume Culture - Bibliometric quantitative and qualitative semantic network approaches - (<복식문화연구>의 지적구조와 연구동향 - 계량정보학적 양적 접근과 의미연결망의 질적 접근 -)

  • Choi, Yeong-Hyeon;Choi, Mi-Hwa
    • The Research Journal of the Costume Culture
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    • v.30 no.4
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    • pp.608-630
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    • 2022
  • The purpose of this study is to examine the relationships between citations and the research trends of The Research Journal of the Costume Culture (RJCC) using bibliometric and network analyses. The results are as follows. First, the RJCC has been cited by a greater number of journals and high-reputation journals today. The RJCC has been mentioned in global academic journals in various fields, and it has been noted the most in environmental science. Second, because of examining the articles published in the RJCC over the past three years (2019 - 2021), it was found that the number of topics was evenly distributed in various subfields of the clothing and textiles sector. The RJCC principally deals with traditional clothing, ethics and sustainability, and technology, which means that the RJCC reflects the past, present, and future. As a result of conducting a cluster analysis using the Wakita-Tsurumi algorithm, the subjects of ethical fashion and sustainability were derived from the subdivisions of the RJCC. This suggests that the RJCC is a journal specialized in ethical fashion and sustainability sectors such as environmental, animal, and labor ethics. This study outlined the current status and future direction of academic journals in the field of clothing through an analysis of the RJCC's influence change and the relationship between citations. In addition, it is academically significant because it identifies research trends and knowledge-structure changes in the apparel science field by identifying changes in research keywords and significant research topics by sector.

Quality standard of Pleurotus ostreatus in a market and changes of mushroom quality during storage (유통 느타리버섯의 등급별 품질규격 및 저장기간별 품질 변화)

  • Lee, Chan-Jung;Oh, Jin-A;Cheong, Jong-Chun;Jhune, Chang-Sung;Moon, Ji-Won;Kong, Won-Sik;Suh, Jang-Sun
    • Journal of Mushroom
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    • v.11 no.4
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    • pp.287-291
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    • 2013
  • This study was carried out to improve standardization of agricultural products and investigate quality changes during storage at different treatments. The standardization does much to improve merchantable quality, distribution efficiency and fair dealings by shipping of the standard agricultural products. Therefore, modification of these standards is required to fit farmhouse situations. Average pileus diameter and thickness of A grades was 38.9 mm and 4.5 mm at shelf culture and 22.2 mm and 3.9 mm at bottle culture. Average stipe length of A grades was 49.6 mm at shelf culture and 66.7 mm at bottle culture, and stipes thick was the highest in A grades of shelf culture. The diameter of pileus of Pleurotus ostreatus stored for 14 days at $4^{\circ}C$ and $10^{\circ}C$ showed no significant difference. but the thickness of pileus decreased rapidly after 7 days. L-value and hardness of stipes and pileus were higher at shelf culture than bottle culture.

In Vitro Tissue Culture Frequency and Transformation of Various Cultivars of Soybean (Glycine max (L.) Merr.) (다양한 콩 자원들의 기내 조직배양 효율 및 형질전환)

  • Seo, Mi-Suk;Cho, Chuloh;Jeong, Namhee;Sung, Soon-Kee;Choi, Man-Soo;Jin, Mina;Kim, Dool-Yi
    • Korean Journal of Plant Resources
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    • v.34 no.4
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    • pp.278-286
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    • 2021
  • Efficient in vitro regeneration system is essential for the successful crop breeding of soybean (Glycine max (L.) Merr.) using the new biotechnology. The genotype of donor plants strongly influences the establishment of tissue culture system. Therefore, the screening of genotypes with excellent tissue culture ability is very important for soybean genetic improvement. In this study, we report the tissue culture efficiency of 21 soybean cultivars belong to Korean soybean core-collection and two foreign cultivars (Jack and Maverick). The Kwangan, Anpyeong and Seonam are share close genetic relationship in 21 cultivars and these three cultivars were observed the high frequency of germination and regeneration. Furthermore, the high tissue culture abilities were also observed in the Williams 82 used in reference genome sequencing and the two foreign cultivars. The transformation of pBAtc:tRNA with bar gene was performed by Agrobacterium tumefaciens in the cultivars with high tissue culture ability. Transformation of the bar gene was identified by PCR analysis in Kwangan, Pungwon, Seonam, and Maverick. Our results provide useful information for the breeding of various soybean cultivars by plant biotechnology such as, genome editing.

Anther Culture of Niger

  • Murthy, H.N.;Ashok Kumar, H.G.;Paek, K.Y.
    • Korean Journal of Plant Tissue Culture
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    • v.27 no.4
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    • pp.353-358
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    • 2000
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