• Title/Summary/Keyword: Research Culture

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A Study on Isolation of BTEX Degrading Microorganism and Variation of BTEX Removal Efficiency and Microorganism Growth Rate According to Co-Culture (BTEX 분해미생물의 순수분리와 혼합 배양에 따른 기질 분해율 및 미생물 성장률 변화에 관한 연구)

  • Chung, Kyung-mi;Lee, Sang-hyup;Lee, Han-woong;Hong, Seok-won;Kim, Young-o;Choi, Yong-su;Yu, Myong-jin
    • Journal of Korean Society on Water Environment
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    • v.21 no.4
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    • pp.347-352
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    • 2005
  • The isolated microorganisms, Pseudomonas stutzeri, Raoultella planticola (Klebsiella), Serratia fonticola from petroleum contaminated soil were enriched on benzene, toluene, ethylbenzene, o-xylene as carbon and energy sources, respectively. And the degradation characteristics of BTEX was observed in the mixed BTEX substrates. We found that the BTEX in mixed substrates were degraded more than 50% by three isolated microorganisms. Among three isolated microorganisms, the highest degradation rate was observed in Pseudomonas stutzeri, but the degradation rate was different according to microorganisms. In order to increase the degradation efficiency, we applied the co-culture of isolated three microorganisms. The mixture rate of pseudomonas stutzeri : Raoultella planticola (Klebsiella) : Serratia fonticola was follows ; 1:2:1, 1:1:2, and 2:1:1, respectively. In two co-culture of 1:2:1 and 1:1:2, degradation rate was lower than isolated microorganisms. However, degradation rate became higher than isolated microorganisms and the degradation rate of benzene, toluene, and ethylene was more than 95% in co-culture of 2:1:1. The degradation rate increased through the co-culture of isolated microorganisms, however, the growth rate decreased. This was resulted from the substrate competition between microorganisms. The co-culture of microorganisms is a effective method to increase the degradation efficiency of BTEX and the co-culture mixing rate is a important factor for determination of degradation efficiency.

Expression characteristic of pop art in Jean-Charles de Castelbajac's works (Jean-Charles de Castelbajac 작품에 나타난 팝아트의 표현 특성)

  • Kim, Sun Young
    • The Research Journal of the Costume Culture
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    • v.22 no.5
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    • pp.688-701
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    • 2014
  • This study examined the expression characteristics in pop art works of Jean-Charles de Castelbajac. The study here aimed at possibility to find a design development in building up the unique art world of creativity based on popularity, artistry, and originality without confinement to the trend only. For the research method, review of literature and analysis about Castelbajac's works reflecting the pop art feature in the collections from 2000S/S to 2012F/W were performed. The results of research are as follows. The external expression form of Castelbajac's works based on pop art was grouped roughly into use of mass culture image, appropriation of pop art expression technique, and parody of art works. First, his work appeared as application of the mass culture image such as symbolic thing in the modern consumer society, object in an ordinary life, character of well-known animation, national flag and famous star. Second, such appropriated pop art techniques showed as pop color in strong primary color and silk screen, photomontage, collage, assemblage, graffiti, and lettering. Third, a variety of images featured earlier in art works were shown in parody. These works are valuable in that they are expressed aesthetically through regeneration of popular culture's various images in view of fashion, they are described in the non-traditional value with frolic resistance and deviation out of existing fashion norm, and they are given the dynamic creativity integrated with art and fashion.

Production and Secretion of Human Interleukin-18 in Transgenic Tobacco Cell Suspension Culture

  • Sharma, Niti;Kim, Tae-Geum;Yang, Moon-Sik
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.11 no.2
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    • pp.154-159
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    • 2006
  • Interleukin-18 (IL-18), otherwise known as interferon-gamma-inducing factor (IGIF), is one of several well characterized and important cytokines that contribute to host defenses. The complementary DNA (cDNA) of mature human interleukin-18 gene (hIL-18) was fused with the signal peptide of the rice amylase 1A gene (Ramy1A) and introduced into the plant expression vector under the control of a duplicated CaMV 35S promoter. The recombinant plasmid was transformed into tobacco (Nicotiana tabacum L. cv Havana) using the Agrobacterium-mediated transformation method. The integration of the hlL-18 gene into the genome of transgenic tobacco plants was confirmed by polymerase chain reaction (PCR) amplification and its expression was observed in the suspension cells that were derived from the transgenic plant callus by using Northern blot analysis. The hlL-18 protein was detected in the extracts of the transgenic callus and in the medium of the transgenic tobacco suspension culture by using immunoblot analysis. Based upon enzyme-linked immunosorbant assay (ELISA) results, the expression level of the hlL-18 protein approximated $166{\mu}g/L$ in the suspension culture medium. Bioassay results from the induction of $interferon-{\gamma}$ from a KG-1 cell line indicated that the hlL-18 secreted into the suspension culture medium was bioactive.

Nursery and Main Culture Conditions for Mass Cultivation of the Brown Alga, Ecklonia cava Kjellman (갈조류 감태 (Ecklonia cava Kjellman)의 대량양식을 위한 가이식 및 양성 조건)

  • Hwang, Eun-Kyoung;Gong, Yong-Geun;Ha, Dong-Su;Park, Chan-Sun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.43 no.6
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    • pp.687-692
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    • 2010
  • The mass cultivation of Ecklonia cava Kjellman was studied as a potential biomass source for the extract industry in Korea. Experiments were conducted to investigate the optimal conditions for artificial seed production and mass cultivation of this species. Maximum growth and young thalli development in the nursery culture area occurred at 2 m depth, whereas maximum growth of thalli in the main culture area occurred at 1 m depth. Production of E. cava was between 2.6 and 3.6 kg wet wt. $m^{-1}$ after depth control and removal of fouling organism, etc. The relationship between optimal water depth for culture and underwater irradiance during the E. cava cultivation was calculated as: y = -0.718x + 8.042 ($r^2$=0.976). The growth rates achieved in this trial indicate that E. cava cultures could produce and supply sufficient biomass.

Effect of Organizational Culture on Corporate Social Welfare Activities

  • JEONG, Young Joo;CHOI, Moon Kyung
    • East Asian Journal of Business Economics (EAJBE)
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    • v.9 no.4
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    • pp.43-54
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    • 2021
  • Purpose - Stakeholders play a vital part in the company's CSR activities and they are part of the company's achievement and affect the company's achievement or business objectives. This study aims to add insight into the already existing knowledge how the organizational culture can promote corporate social welfare activities. Research design, Data, and methodology - The current authors obtained text data for the possible practical suggestions which might be used for the creation of coding method. That implies that the present author investigated only trustable textual sources to provide for the possible solutions such as peer-revied sources and published book. Result - Research results indicated that organizational culture promotes corporate social welfare activities by making people know their values and understand how they come about. Not every community knows what its members want and how to achieve its needs. Sometimes, a community can obtain the values and principles of an organization and incorporate them into community values. Conclusion - Executive leadership and customers are part of society. Any strategy that influences their operation and works ethic influences the contact of the community. This research found methods vital in setting up an excellent culture that enhances profitability and the corporate social welfare activities through motivation and communication.

Influence of National Culture on Staff Preferences to Knowledge Sharing Practices: the Case of Saudi Arabia

  • Al Hazmi, Hassan S.J.;Mohamed, Sherif
    • International conference on construction engineering and project management
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    • 2015.10a
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    • pp.454-458
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    • 2015
  • A number of practices for sharing knowledge has been recommended in the literature. These practices, however, are considered to be applicable, universally. This assumption is challenged by this paper which empirically explores the relationship between national culture and knowledge sharing practices in the context of procuring educational buildings in Saudi Arabia. The paper reports on a research study with the aim to identify whether national culture dimensions could be significant variables that impact upon staff preferences in relation to project knowledge sharing practices. The paper critically reviews the literature to identify appropriate measures for knowledge sharing practices. It explores the means by which public sectors professional exchange project knowledge; evaluates policies and level of technical support needed to facilitate knowledge sharing, then proceeds to how employees perceive the benefits they will gain by practicing project knowledge sharing, and their role in this process. The paper tests the relationships among the research constructs based on data collected from 115 project managers responsible for the procurement of educational buildings in Saudi Arabia. The paper provides empirical evidence that the national culture has a significant influence on staff preferences. In light of the research findings the paper concludes with practical recommendations for project knowledge sharing practices that are in line with staff preferences and their cultural orientation. The recommendations should facilitate a more effective application of knowledge sharing practices.

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Bioluminescent Determination of Lactose Secretion: A Measure of the In Vitro Performance of Mammary Acini from Lactating Rats

  • Choi, B.H.;Stewart, K.W.;Davis, S.R.;Myung, K.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.2
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    • pp.274-278
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    • 2002
  • A culture system for lactating rat mammary acini was evaluated, where the primary indicator of performance was lactose secretion, measured by a sensitive bioluminescence assay. Lactose secretion was reduced by half (p<0.01) over the first 6 h of culture by overnight feed withdrawal (FW) from tissue donors but was sensitive to increased glucose concentration in the culture media (p<0.001) up to 30 mM. Lactose production of cells from fed donors over the first 6 h in culture in 30 mM glucose was 8.9 fmol/cell/h - a rate calculated to be about half that in vivo. No significant difference was shown in lactose secretion by cells from fed or FW rats over 6-24 h. Lactose secretion was 3.6 fmol/cell/h by cells from fed animals in 40 mM glucose concentration media over the 6-24 h culture period. Addition of insulin to the culture media had no effect on rates of lactose secretion while addition of prolactin and hydrocortisone, with or without insulin, significantly (p<0.001) decreased lactose production over both 0-6 h and 6-24 h culture periods. Lactose synthesis in vitro was significantly enhanced by aeration of the media during collagenase digestion of mammary tissue (p<0.05). No improvement in lactose secretion was effected by shaking of cells during culture, Matrigel coating of culture dishes or change in cell density over a range up to 2.5 million cells per ml.

A Study on Improvement of Organizational Culture of the Members of Manufacturing and Service industry Quality Control : Focused on Mediation Effect of Job Satisfaction (제조업과 서비스업 품질관리 종사원들의 조직문화 개선에 관한 연구 : 직무만족의 매개변수를 중심으로)

  • Lee, Chul Woo;Shin, Yong Ho;Shang, Meng;Ryu, Young Shin
    • Journal of Korean Society for Quality Management
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    • v.48 no.1
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    • pp.29-50
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    • 2020
  • Purpose: Targeting the members of manufacturing and Service industry Quality Control team this study is going is to carry out research about whether the outcome of OCB(Organizational Citizenship Behaviors) and organization can be brought about by organizational culture that is suitable for them. this study tries to identify the direct・indirect causal relationship between these variables and OCB by selecting organizational culture as a leading variable and job satisfaction as a parameter. Methods: SPSS 22.0 was used for data analysis and AMOS 18.0 statistical program for structural equation model analysis. For the descriptive statistics this study verified reliability analysis, feasibility analysis, structural equation model analysis, research hypothesis, and mediating effects. Results: As a result of path analysis estimating the regression coefficients for the linear structure analysis of the correlations between variables for the hypothesis verification, the rational culture among the organizational culture types of the manufacturing Quality Control team showed a positive (+) effect on the job satisfaction, and hierarchical culture has negative(-) effect on job satisfaction. Conclusion: This study suggested that the composition and friendly behavior of desirable organizational culture has a very close relationship in connection between job satisfaction and OCB by examining the causal relationship between OCB for improvement activities for organizational culture by establishing the organizational culture and job satisfaction of the manufacturing Quality Control team.

Development of the Educational Program for Korean Traditional Food Culture for the Elementary Students (초등학교 고학년을 대상으로 한 한국 전통음식문화 교육 프로그램 개발)

  • Yang, Il-Seon;Jeong, Hye-Gyeong;Jang, Mun-Jeong;Cha, Jin-A;Lee, So-Jeong;Jeong, Ra-Na;Park, So-Hyeon;Cha, Seong-Mi
    • Journal of the Korean Dietetic Association
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    • v.12 no.2
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    • pp.127-145
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    • 2006
  • The purpose of this study is to develop an educational program for Korean traditional food culture, which is consisted of diverse activity factors and educational contents of the elementary students, and to transfer Korean traditional food culture to the next generation effectively. We have developed the program on the basis of FIB(Fun Integrated Behavior) approach derived from the activity-based education theory. This program is consisted of 32 subjects related with Korean traditional food culture, which is planned to perform in special activity classes for 2 semesters. An instruction guidebook for teachers and a workbook for students have been also developed. The traditional food culture educational program which is developed in this study could be used as a practical educational tool in 'dietary life and culture class' or 'special activity class' which is recently being introduced as the nutrition education curriculum in the elementary school. Furthermore, we expect this program to be able to contribute to transferring Korean traditional food culture to the next generation and reinforcing the elementary schoolers' diet behavior through inspiring them with a realization of the superiority of Korean traditional food culture.

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