• Title/Summary/Keyword: Research Culture

Search Result 14,995, Processing Time 0.038 seconds

Production of Humanised Anti-hepatitis B Antibody in Butyrate-Treated Chinese Hamster Ovary Cells (Butyrate처리된 차이니즈 햄스터 난소세포에서 Hepatitis B 바이러스 인간화항체의 생산)

  • Park Se-Cheol;Lee Jae-Sun;Lee Byung-Kyu;Kang Heui-Il
    • Microbiology and Biotechnology Letters
    • /
    • v.34 no.1
    • /
    • pp.47-51
    • /
    • 2006
  • Sodium butyrate (NaBu) is used as an enhancer for the production of recombinant proteins in Chinese hamster ovary (CHO) cells. However, NaBu is well-known for its cytotoxic effect, thereby inducing apoptosis. CHO cells which had been engineered to express a humanised anti-HBV antibody were cultured using serum-free medium, Ex-cell 301. From a seeding density of $2{\times}10^5$ cells/ml, CHO cells grown with serum-free medium reached a maximum cell density of $1.3{\times}10^6$ cells/ml after 9 days in culture and produced a maximal antibody concentration of 130 mg/l after 13 days in culture. In the perfusion culture system, CHO cells producing anti-HBV antibody grown in an 7.5 1 bioreactor seeded with $2{\times}10^5$ cells/ml reached a maximal antibody concentration of 85 mg/1 after 720 h in culture. The addition of 0.3 mM NaBu and lowering culture temperature to $33^{\circ}C$ elongated the culture period to 60 days and increased the production yield by 2-fold, compared to control culture.

A Study on the Context Analysis of Dining Culture in Yam Cha (얌차(飮茶)의 식문화 정황분석 연구)

  • Jeung, Ji-Hong;Jeung, Ji-Eun;Seok, Wang-Mi
    • Journal of the HCI Society of Korea
    • /
    • v.4 no.1
    • /
    • pp.29-34
    • /
    • 2009
  • The food culture has been advanced with technical developments. The technical advances of food refrigeration and refrigerated transport system enable people to enjoy raw and fresh foods in the middle of dessert and in current days the development of information technology helps people to get variable information as like the information of food stuffs, freshness, the history of cooking, other useful information. We need to research the information architecture of food culture, which means Information technology grafts on the food culture, to provide diverse services that information technology utilized. As the first step of our research, we define the context of food culture for integration of food culture and Information technology, and find out the relationships between each factors. This research is considered the food culture as a medium between human to human relationships and the food, and redefined it with a relationship viewpoint. We've selected Yam cha that is one of the famous foods in southern China as a research item and referenced data that we researched with students in Guangzhou College of Art, China.

  • PDF

Organizational Culture And Emotional Intelligence As Predictors Of Job Performance Among Library Personnel In Academic Libraries In Edo State, Nigeria

  • Igbinovia, Magnus O.;Popoola, S.O.
    • Journal of Information Science Theory and Practice
    • /
    • v.4 no.2
    • /
    • pp.34-52
    • /
    • 2016
  • This study was designed to investigate organizational culture and emotional intelligence as predictors of job performance among library personnel in Edo state, Nigeria. The survey research design was employed for the study with a population size of 181 library personnel in the 15 academic libraries under study, and due to the manageable population size, total enumeration was adopted as the sampling technique. The questionnaire was used to elicit data from the respondents. Of the 181 copies of the questionnaire administered, 163 copies were retrieved and found valid for analysis constituting a 90% response rate. Four research questions and four null hypotheses (tested at 0.05 level of significance) were formulated to guide the study. The tool used to analyze the research question was descriptive statistics (percentage, mean, and standard deviation) and inferential statistics (correlation and multiple regression) for testing the hypotheses. The findings of the study revealed that there is a high level of job performance, good organizational culture, and high level of emotional intelligence among the personnel. Organizational culture and emotional intelligence jointly and significantly predict job performance of personnel. There is significant positive correlation between organizational culture and job performance. The linear combination of emotional intelligence and organizational culture predict job performance of library personnel in the academic libraries under study. The research concludes that there is a need for high job performance in libraries which is predicted by the organizational culture of the library and the level of emotional intelligence of the library personnel.

A Study on Flexible Culture in Aviation Maintenance Organization (항공정비조직의 유연성문화 연구)

  • Kim, Chun-Yong
    • Journal of the Korean Society for Aviation and Aeronautics
    • /
    • v.23 no.1
    • /
    • pp.1-7
    • /
    • 2015
  • The aircraft maintenance demands a lot of aviation mechanics in urgent according to the high season and low season in seasonal demand adjusted, unplanned maintenance work such as AD (airworthiness directives) and troubleshooting of aircraft system fault. The advent of Super-Large Aircraft such as A380 with aircraft materials, power plant and avionics systems which were not in previous models must be expected to increase the complexity of the aircraft maintenance system. This study is intended to propose the development plan for formation of positive flexibility culture in aircraft maintenance organization through the review of previous research on flexibility culture of organization and through empirical research for bureaucratic and poor factors by the evaluation of flexibility culture level of domestic aircraft maintenance organization.

Research about Competitive Power High Position of Food-Service Industry - Laying Stress on Eating Out Company Interior Factor - (외식기업의 경쟁력 강화 요인에 관한 연구 - 외식기업 내부요인을 중심으로 -)

  • 유택용;박면애
    • Culinary science and hospitality research
    • /
    • v.10 no.3
    • /
    • pp.83-96
    • /
    • 2004
  • According to construction of global administration environment, all over the world was ended up in infinite competition period. Because eating out company also competes with another company, it must enjoy competitive power high position to secure high position continuously, classified by formation constituent's individuation and two aspects of organic special quality by the factor. Individuation classified by formation constituent's business ability, personal relations, age factors of Information Technology, and organic special quality classifies by factor of formation culture and result that analyze eating out company's business condition competitive power reinforcement effect factor is same as following. First, was construed by company culture, personal relations, business ability period of ten days with family Restaurant, and Information Technology showed that do not influence. Competitive power reinforcement effect factor with special restaurant was construed by company culture, personal relations, business ability period of ten days, and fast food company culture and Information Technology factor with hotel restaurant company culture by competitive power reinforcement effect factor construe.

  • PDF

Research on Four Variables toward the Effective Integration of Culture in the EFL Class of Korea

  • Roh, Seung-Bin
    • English Language & Literature Teaching
    • /
    • v.11 no.2
    • /
    • pp.91-110
    • /
    • 2005
  • Many Korean EFL (English as a Foreign Language) students do not have sufficient opportunity to develop cultural knowledge and information in their classrooms. EFL teachers also tend to ignore the teaching of culture. Even though culture is taught, it simply tends to deliver "fact-only" information from the viewpoint of a "tourist level rather than cultural awareness by comparing native with target cultural references. Teaching target cultural knowledge and information should be delivered within the native cultural frame, and teaching of culture must be an integral part of teaching and learning English. The research methodology was quantitative. Quantitative data was gathered from 83 Korean EFL teachers and 286 EFL students by questionnaire. Findings indicated that three of these independent variables (cultural inequality, English-only instruction, and Unoism) were significantly and inversely related to integration of culture.

  • PDF

CULTIVATION OF STUDENTS' CORE LITERACY BASED ON THE MATHEMATICAL CULTURE PERSPECTIVE

  • Nan, Hua;Tong, Shan;Xu, Yu
    • East Asian mathematical journal
    • /
    • v.35 no.2
    • /
    • pp.163-172
    • /
    • 2019
  • In recent years, the term core literacy has been popular in educational circles all over the world, and there are more and more research on core literacy. At the same time, the mathematical culture has also been highly valued on a global scale. The mathematical culture is a part of college students' cultural quality, and then, what is the connection between the mathematical culture and mathematical core literacy? How to improve the mathematical core literacy of contemporary college students? This paper gives the corresponding answers to these two questions. By illustrating the concrete implementation of the course "Mathematical Culture" offered by Yanbian University, this paper discusses various measures for cultivating students' core qualities in Chinese universities. It must be useful and promote the research on mathematical core literacy for the educators in various countries.

Analysis on the Effects of National culture to International firm's Leadership styles and organizational Performance (국제기업의 리더십 유형과 조직유효성에 국가문화특성이 미치는 영향에 대한 분석)

  • Lee, Jungah
    • International Commerce and Information Review
    • /
    • v.16 no.3
    • /
    • pp.381-412
    • /
    • 2014
  • Un globalization unfolds, concerns and research toward the differnces international cultures has increased. However, there is a dearth of research regarding how to nature culture align orgainzational performance with leadership style. Accordingly, this study aims to identify the relationship between leadership style and the orgainzational performance with mediating effect of national culture. Understanding the cultures of other countries is not as fast as the speed of internationalization. As globalization advances, interest and research on the characteristics of national culture differences have been increasing. One of the important challenges for business leaders is the ability to understand and deal with cross-cultural management. But there is a lack of research on cross-cultural management. In order to achieve this objective, this study, adopts Hofstede cultural dimensions, and finds the mediating effect on these transformational and transactional leadership on organizational performance.Feminism, collectivism, power distance, and uncertainty avoidance were classified into four factors of national culture has differnct mediating effect on leadership style and performance. This research has concentrated on leadership-styles and organizational performance with mediating effect of national culture.

  • PDF

Antagonistic Potential of Native Trichoderma viride Strain against Potent Tea Fungal Pathogens in North East India

  • Naglot, A.;Goswami, S.;Rahman, I.;Shrimali, D.D.;Yadav, Kamlesh K.;Gupta, Vikas K.;Rabha, Aprana Jyoti;Gogoi, H.K.;Veer, Vijay
    • The Plant Pathology Journal
    • /
    • v.31 no.3
    • /
    • pp.278-289
    • /
    • 2015
  • Indigenous strains of Trichoderma species isolated from rhizosphere soils of Tea gardens of Assam, north eastern state of India were assessed for in vitro antagonism against two important tea fungal pathogens namely Pestalotia theae and Fusarium solani. A potent antagonist against both tea pathogenic fungi, designated as SDRLIN1, was selected and identified as Trichoderma viride. The strain also showed substantial antifungal activity against five standard phytopathogenic fungi. Culture filtrate collected from stationary growth phase of the antagonist demonstrated a significantly higher degree of inhibitory activity against all the test fungi, demonstrating the presence of an optimal blend of extracellular antifungal metabolites. Moreover, quantitative enzyme assay of exponential and stationary culture filtrates revealed that the activity of cellulase, ${\beta}$-1,3-glucanase, pectinase, and amylase was highest in the exponential phase, whereas the activity of proteases and chitinase was noted highest in the stationary phase. Morphological changes such as hyphal swelling and distortion were also observed in the fungal pathogen grown on potato dextrose agar containing stationary phase culture filtrate. Moreover, the antifungal activity of the filtrate was significantly reduced but not entirely after heat or proteinase K treatment, demonstrating substantial role of certain unknown thermostable antifungal compound(s) in the inhibitory activity.