• Title/Summary/Keyword: Reproduction Technology

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Differences in liver microRNA profiling in pigs with low and high feed efficiency

  • Miao, Yuanxin;Fu, Chuanke;Liao, Mingxing;Fang, Fang
    • Journal of Animal Science and Technology
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    • v.64 no.2
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    • pp.312-329
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    • 2022
  • Feed cost is the main factor affecting the economic benefits of pig industry. Improving the feed efficiency (FE) can reduce the feed cost and improve the economic benefits of pig breeding enterprises. Liver is a complex metabolic organ which affects the distribution of nutrients and regulates the efficiency of energy conversion from nutrients to muscle or fat, thereby affecting feed efficiency. MicroRNAs (miRNAs) are small non-coding RNAs that can regulate feed efficiency through the modulation of gene expression at the post-transcriptional level. In this study, we analyzed miRNA profiling of liver tissues in High-FE and Low-FE pigs for the purpose of identifying key miRNAs related to feed efficiency. A total 212~221 annotated porcine miRNAs and 136~281 novel miRNAs were identified in the pig liver. Among them, 188 annotated miRNAs were co-expressed in High-FE and Low-FE pigs. The 14 miRNAs were significantly differentially expressed (DE) in the livers of high-FE pigs and low-FE pigs, of which 5 were downregulated and 9 were upregulated. Kyoto Encyclopedia of Genes and Genomes analysis of liver DE miRNAs in high-FE pigs and low-FE pigs indicated that the target genes of DE miRNAs were significantly enriched in insulin signaling pathway, Gonadotropin-releasing hormone signaling pathway, and mammalian target of rapamycin signaling pathway. To verify the reliability of sequencing results, 5 DE miRNAs were randomly selected for quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The qRT-PCR results of miRNAs were confirmed to be consistent with sequencing data. DE miRNA data indicated that liver-specific miRNAs synergistically acted with mRNAs to improve feed efficiency. The liver miRNAs expression analysis revealed the metabolic pathways by which the liver miRNAs regulate pig feed efficiency.

Characterization of Embryonic Feather Follicle Development in the Chinese Indigenous Jilin White Goose

  • Wu, W.;Xu, R.F.;Li, C.H.;Wu, C.X.
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.3
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    • pp.346-352
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    • 2008
  • To investigate goose feather follicle development and difference among the dorsal, ventral, and thoracal tracts during embryonic stage, the present study was conducted on 180 embryos at different ages obtained from the Jilin White goose, a Chinese indigenous breed. The study indicated that the epidermis and dermis of goose embryo formed between embryonic day 10 (E10) and 12 (E12). The thickness of the epidermis remained unchanged until hatching; while the thickness of the dermis increased throughout embryonic development. The primary feather follicles formed around E13-E14 and there were no new primary feather follicles forming after E18. The secondary feather follicles formed coincidently at E18. The density of primary and secondary feather follicles on the ventral and thoracal tracts were significantly higher than those on the dorsal tract (p<0.05). For primary and secondary follicles, the diameter of the feather bulbs and the depth of the feather follicles on the dorsal tract were much greater than those on the thoracal and ventral tracts (p<0.01), respectively; while the difference between the ventral and thoracal tracts was not significant (p>0.05). It is concluded that the Jilin White goose is of a single-follicle group structure, differing from mammals which are of multiple-follicle group structure.

A Study on the Angular Characteristics of Photopolymer-based Hologram Recording and Reproducing Light

  • Kwang-pyo, Hong;Jiwoon, Lee;Lee-hwan, Hwang;Soon-chul, Kwon;Seunghyun, Lee
    • International Journal of Advanced Culture Technology
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    • v.10 no.4
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    • pp.460-469
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    • 2022
  • Increasing interest in the metaverse world these days, interest in realistic content such as 3D displays is growing. In particular, hologram images seen in movies provide viewers with an immersive display that cannot be seen in conventional 2D images. Since the first discovery of holography by Dennis Gabor in 1948, this technology has developed rapidly. Spatially, this beginning of technology like Optical hologram called analog hologram and Digital hologram such as computer-generated hologram (CGH). In analog and digital holograms, a recording angle and a recording wavelength are having important role when reproducing and display hologram. In the hologram, diffraction of light causes by unexpected formed by the synthesis from interference with object and reference light. When recording, the incident light information and mismatched reproduction light reconstruct the hologram in an undesirable direction. Reproduction light that is out of sync with incident light information with initial condition of recording will cause reconstructed image in an undesirable direction. Therefore, we analyze the holographic interference pattern generated by hologram recording in volume holograms using photopolymer and analyze the characteristics that vary depending on the angle of the reproduced light. This is expected to be used as a basic research on various holographic application that may cause as holograms are applied to industries in the future.

Effects of algae on chronic assessment endpoints observed with Korean freshwater Cladocerans (한국산 물벼룩의 먹이조건별 번식영향)

  • Kim, Byung-Seok;Park, Yoen-Ki;Shin, Jin-Sup;Kim, Jin-Hwa;Ahn, Young-Joon
    • The Korean Journal of Pesticide Science
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    • v.8 no.2
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    • pp.117-128
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    • 2004
  • The influence of different feeding rates of three green algae on growth and reproduction in four Korean cladocera, Daphnia sp., Daphnia obtusa, Moina macrocopa, and Simocephalus vetulus was investigated. The growth and reproduction rates of Daphnia sp. fed Chlorella vulgaris cells were significantly increased than those of daphnid fed Senedesmus subspicatus and Selenastrum carpricornutum. The total offsprings of Daphnia sp. fed $25\times10^4$ and $50\times10^4$ cells Chlorella vulgaris for 21days were $124{\pm}2.8$ and $168{\pm}22.1$, respectively. The Daphnia obtusa fed $25\times10^4$ and $50\times10^4$ cells Selenastrum carpricornutum was shown good reproduction rates, $72.2{\pm}7.9$ and $79.6{\pm}22.9$, respectively. The Moina macrocopa fed $25\times10^4$ and $50\times10^4$ cells Senedesmus subspicatus as well as Simocephalus vetulus and $25\times10^4$ and $50\times10^4$ cells Chlorella vulgaris were shown good reproduction rates. All of algae tested were sufficient as a good diet for Korean Cladocerans in this study. However, the preferred alga was Chlorella vulgaris and the optimal feeding concentrations were $2.5\times10^5\sim5\times10^5$ cells/mL, because the alga was shown most stable and generally high production rates in all cladocerans tested.

A Study on the Abnormal and Fault Reproduction Method for Smart Monitoring of Thrust Bearing in Wave Power Generation System (파력발전 시스템 쓰러스트 베어링의 스마트 모니터링을 위한 이상 및 고장 운용 재현 방법에 관한 연구)

  • Oh, Jaewon;Min, Cheonhong;Sung, Kiyoung;Kang, Kwangu;Noh, Hyon-Jeong;Kim, Taewook;Cho, Sugil
    • Journal of the Korean Society of Industry Convergence
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    • v.23 no.5
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    • pp.835-842
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    • 2020
  • This paper considers a method of reproducing abnormal and fault operation for smart monitoring of thrust bearing used in wave power generation system. In order to develop smart monitoring technology, abnormal and failure data of actual equipment are required. However, it is impossible to artificially break down the actual equipment in operation due to safety and cost. To tackle this problem, a test bed that can secure data through reproduction of a faulty operating environment should be developed. Therefore, in this study, test bed that can reproduce each situation was developed and the operation result was analysis after identifying the situation to be reproduced through the failure factor analysis of the thrust bearing.

The interpretation of Korean orientalism as the practice of globalization of tradition - The study on the designers Kim Jihaye and Lee Jeanyoun's works from 2000 to 2012 - (전통 세계화의 실천으로서 한국적 오리엔탈리즘(Orientalism)의 해석 - 2000년부터 2012년까지 디자이너 김지해·이진윤의 작품을 중심으로 -)

  • Choi, Seungyeun
    • The Research Journal of the Costume Culture
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    • v.28 no.2
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    • pp.245-264
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    • 2020
  • This study identifies Kim Jihaye and Lee Jeanyoun as actor-networks with cultural values and meanings. It analyzes the process of reorganizing their works into Korean orientalism via an action of co-construction with formative techniques. First, the historical context of orientalism formed in the fashion world will be studied, followed by the design characteristics of orientalism, and the correlation between the Korean orientalism of these designers on the world stage influenced by the globalization discourse of Korean tradition since the 1980s. Next, works of Kim Jihaye and Lee Jeanyoun from 2000 to 2012 will be analyzed, revealing aspects of Korean image reproduction. Consequently, this study finds that the Korean image is restructured socio-culturally through technological imagery as a reproduction of reality by the production subject. This study reminds us of the reflective and cultural meaning of fashion designers in the area of image reproduction, sociocultural practice, and materials and technology.

DYNAMICAL BEHAVIOUR OF A DRINKING EPIDEMIC MODEL

  • Sharma, Swarnali;Samanta, G.P.
    • Journal of applied mathematics & informatics
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    • v.31 no.5_6
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    • pp.747-767
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    • 2013
  • In this paper we have constructed a mathematical model of alcohol abuse which consists of four compartments corresponding to four population classes, namely, moderate and occasional drinkers, heavy drinkers, drinkers in treatment and temporarily recovered class. Basic reproduction number $R_0$ has been determined and sensitivity analysis of $R_0$ indicates that ${\beta}1$ (the transmission coefficient from moderate and occasional drinker to heavy drinker) is the most useful parameter for preventing drinking habit. Stability analysis of the model is made using the basic reproduction number. The model is locally asymptotically stable at disease free or problem free equilibrium (DFE) $E_0$ when $R_0&lt;1$. It is found that, when $R_0=1$, a backward bifurcation can occur and when $R_0&gt;1$, the endemic equilibrium $E^*$ becomes stable. Further analysis gives the global asymptotic stability of DFE under some conditions. Our important analytical findings are illustrated through computer simulation. Epidemiological implications of our analytical findings are addressed critically.

Effects of Enhanced pCO2 and Temperature on Reproduction and Survival of the Copepod Calanus sinicus

  • Kang, Hyung-Ku;Lee, Chang-Rae;Kim, Dongseon;Yoo, Sinjae
    • Ocean and Polar Research
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    • v.38 no.4
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    • pp.303-314
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    • 2016
  • We tested the combined effects of increased partial pressure of $CO_2$ ($pCO_2$) and temperature on the reproduction and survival of the copepod Calanus sinicus from Asan Bay, the Yellow Sea under laboratory conditions to understand the impact of acidification on copepods. Egg production rate, survival rate, and fecal pellet production of C. sinicus were not affected by 1305 ppm $pCO_2$ or with combined treatments of temperature and $pCO_2$, including $8^{\circ}C$ and 289 ppm $pCO_2$ (ambient), $8^{\circ}C$ and 753 ppm $pCO_2$ (high $pCO_2$), $12^{\circ}C$ and 289 ppm $pCO_2$ (high temperature), and $12^{\circ}C$ and 753 ppm $pCO_2$ (greenhouse), for 5 or 10 d of exposure. However, egg hatching success of C. sinicus decreased significantly in the greenhouse treatment compared with the ambient or the high $pCO_2$ treatments. These results suggest that a combined treatment ($pCO_2$ and temperature) affected egg viability more than a single treatment($pCO_2$).

Supplementation of retinoic acid alone in MSC culture medium induced germ cell-like cell differentiation

  • Kuldeep Kumar;Kinsuk Das;Ajay Kumar;Purnima Singh;Madhusoodan A. P.;Triveni Dutt;Sadhan Bag
    • Journal of Animal Reproduction and Biotechnology
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    • v.38 no.2
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    • pp.54-61
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    • 2023
  • Background: Germ cells undergo towards male or female pathways to produce spermatozoa or oocyte respectively which is essential for sexual reproduction. Mesenchymal stem cells (MSCs) have the potential of trans-differentiation to the multiple cell lineages. Methods: Herein, rat MSCs were isolated from bone marrow and characterized by their morphological features, expression of MSC surface markers, and in vitro differentiation capability. Results: Thereafter, we induced these cells only by retinoic acid supplementation in MSC medium and, could able to show that bone marrow derived MSCs are capable to trans-differentiate into male germ cell-like cells in vitro. We characterized these cells by morphological changes, the expressions of germ cell specific markers by immunophenotyping and molecular biology tools. Further, we quantified these differentiated cells. Conclusions: This study suggests that only Retinoic acid in culture medium could induce bone marrow MSCs to differentiate germ cell-like cells in vitro. This basic method of germ cell generation might be helpful in the prospective applications of this technology.

3D TV by electro-holography -- Fast hologram calculation by networking --

  • Koki-SATO;Kunihiko-TAKANO;Takeshi-KOYAMA
    • Proceedings of the Korean Society of Broadcast Engineers Conference
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    • 1998.06b
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    • pp.63-66
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    • 1998
  • Recently, a study of Virtual-Reality becomes active. Expectation to 3-D reproduction technology rises with it. Electro-holography using Spatial-light-modulator attracts attention, because natural 3-D animated image is provided. It is important to execute hologram calculation at high speed to realize the real time electro-holography. In other words, a higher-speed computer and a study of calculation algorithm becomes an important subject. This time, we realized to fast calculation of Fresnel-Hologram by networking technology.

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