• Asian-Australasian Journal of Animal Sciences
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• v.15 no.3
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• pp.445-452
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• 2002

The main objective of modern reproductive technologies in pig reproduction is to increase reproductive efficiency and rates of genetic improvement. They also offer potential for greatly extending the multiplication and transport of genetic materials and the conservation of unique genetic resources in reasonably available forms for possible future use. The development and refinement of these technologies is concentrating on gamete and embryo collection, sorting and preservation, in vitro production of embryos, culturing, manipulation of embryos (splitting, nuclear transfer, production of chimeras, establishment embryo stem cells, and gene transfer) and embryo transfer. Also, the development of these novel technologies is facilitated by modern equipment for ultrasonography, microscopy, cryopreservation, endoscopy, and flow cytometry, microinjectiors, micromanipulators and centrifugation. The real impact on herd productivity will come from combining new reproductive techniques with powerful DNA technologies. The new reproductive techniques will allow a rapid turnover of generations, whereas the DNA technology can provide selection, which does not need phenotypic information when the selection decisions are made.

• Journal of Animal Reproduction and Biotechnology
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• v.34 no.2
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• pp.65-69
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• 2019

Since the development of the first genetically-modified mouse, transgenic animals have been utilized for a wide range of industrial applications as well as basic research. To date, these transgenic animals have been used in functional genomics studies, disease models, and therapeutic protein production. Recent advances in genome modification techniques such zinc finger nuclease (ZFN), transcription activator-like effector nucleases (TALEN), and clustered regularly interspaced short palindromic repeats (CRIPSR)-Cas9, have led to rapid advancement in the generation of genome-tailored livestock, as well as experimental animals; however, the development of genome-edited poultry has shown considerably slower progress compared to that seen in mammals. Here, we will focus primarily on the technical strategies for production of transgenic and gene-edited chickens, and their potential for future applications.

• Clinical and Experimental Reproductive Medicine
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• v.39 no.2
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• pp.41-45
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• 2012

Transcription factors govern diverse aspects of cell growth and differentiation as major switches of gene expression. Etv5, a member of the E26 transformation-specific family of transcription factors, has many stories to share when it comes to reproduction. Etv5 deficient mice show complex infertility phenotypes both in males and females. In males, the infertility phenotype exhibited by Etv5 deficiency is sexually dimorphic, and it involves both somatic cells and germ cells. In $Etv5^{-/-}$ female mice, the problem is more complicated by hormonal involvement. This review synthesizes old and new information on this versatile transcription factor-from the inadvertent discovery of its role in the testes to its newly discovered role in maintaining spermatogonial stem cells.

• Journal of Industrial Technology
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• v.19
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• pp.351-359
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• 1999

If the tactile sense is introduced to engineering and industries, it may provide more realistic virtual tactile sensing to human and it is possible to develop product that satisfy various consumer's taste. This paper presents a compliance emulator system as a new concept of tactile reproduction simulator which uses magnetic levitation in order to minimize friction and emulates compliance only along the vertical direction. Compliance is one of the important mechanical properties of the object related to tactile sensing of the human. The implemented system equipped with an analog LVDT sensor for a position sensor and employs a PD control with gravity compensation to emulate the specified compliance. To compensate the limited range of the system, the method of attaching the spring with various magnitude of stiffness to the system is adopted and its preliminary test is performed to confirm the validity of the method.

• Journal of Korea Multimedia Society
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• v.24 no.2
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• pp.336-343
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• 2021

AI technology is spreading not only to industrial fields, but also to culture, art, and content fields. In this paper, we proposed a system based on AI technology that can automate the process of reproducing contents using characters for movie contents. After creating the basic appearance of the character by using the StyleGAN2 model from the video extracted from the movie contents, analyzing the character's personality and propensity using the extracted dialogue data, it was determined from the contemplative appearance based on the yin-yang and five elements to the character's propensity. Accordingly, the external characteristics are reflected in the character. Using the OpenPose model, a character's motion is created, and the finally generated data is integrated to reproduce the content. It is expected that many movie contents can be reproduced through the study of the proposed system.

• The Korean Journal of Pesticide Science
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• v.10 no.3
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• pp.196-200
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• 2006

In this study we investigated fecundity and survivorship of four staple Korean freshwater cladocerans for their whole life to develop a new standard method used for ecological risk assessment of pesticide. The tested Korean freshwater cladocerans were Daphnia obtusa, Daphnia sp., Moina macrocopa and Simocephalus vetulus. In addition, standard test species, Daphnia magna endorsed formally by the major international organizations was tested together in order to compare with the reproductive characteristics of Korean cladocerans. A total of 358 young was produced by D. magna, whereas, 297 young was reduced by Daphnia sp. throughout the entire life. The average life span of Daphnia magna was 50 days much longer than any other Korean species. Nevertheless all of the Korean water flea tested showed good fecundity, produced over 60 young for 21 days as the validation criterion for reproduction toxicity test in Ecological Effects Test Guidelines published by USEPA. Especially Moina macrocopa produced their first brood in 4 days. Therefore 10 day reproduction test with Moina macrocopa may be an alternative good method to save cost and time to elucidating the effects of hazardous substances on the reproduction of aquatic invertebrates.

• Journal of Veterinary Science
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• v.24 no.1
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• pp.4.1-4.16
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• 2023

Background: In vitro culture of preantral follicles is a promising technology for fertility preservation. Objectives: This study aims to investigate an optimized three-dimensional (3D) fetal bovine serum (FBS)-free preantral follicle culture system having a simple and easy operation. Methods: The isolated follicles from mouse ovaries were randomly divided in an ultra-low attachment 96-well plates supplement with FBS or bovine serum albumin (BSA) culture or encapsulated with an alginate supplement with FBS or BSA culture. Meanwhile, estradiol (E₂) concentration was assessed through enzyme-linked immunosorbent assay of culture supernatants. The diameter of follicular growth was measured, and the lumen of the follicle was photographed. Spindle microtubules of oocytes were detected via immunofluorescence. The ability of oocytes to fertilize was assessed using in vitro fertilization. Results: The diameters were larger for the growing secondary follicles cultured in ultra-low attachment 96-well plates than in the alginate gel on days 6, 8, and 10 (p < 0.05). Meanwhile, the E2 concentration in the BSA-supplemented medium was significantly higher in the alginate gel than in the other three groups on days 6 and 8 (p < 0.05), and the oocytes in the FBS-free system could complete meiosis and fertilization in vitro. Conclusions: The present study furnishes insights into the mature oocytes obtained from the 3D culture of the preantral follicle by using ultra-low attachment 96-well plate with an FBS-free system in vitro and supports the clinical practices to achieve competent, mature oocytes for in vitro fertilization.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2002.05a
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• pp.453-454
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• 2002

• 한국정보디스플레이학회:학술대회논문집
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• 2003.07a
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• pp.137-141
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• 2003

CRTs can survive in the long run for TV applications, because of their realistic reproduction of live images. CRT technology development has to be directed towards form factor, but also attention has to be paid to the form design of sets. The CRT is the only technology allowing a doubly curved screen surface.

• Journal of Information Display
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• v.4 no.1
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• pp.38-42
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• 2003

CRTs can survive in the long run for TV applications, because of their realistic reproduction of live images. CRT technology development has to be directed towards form factor, but also attention has to be paid to the form design of sets. The CRT is the only technology allowing a doubly curved screen surface.