• 제목/요약/키워드: Replication ratio

검색결과 109건 처리시간 0.047초

메탄가스 전환 미생물촉매 개량을 위한 플라스미드 복제 시작점 예측 (Predicting Plasmid Replication Origin for Methane-converting Microbial Catalyst Improvement)

  • 김민식
    • 신재생에너지
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    • 제19권4호
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    • pp.46-52
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    • 2023
  • Methane is the second most emitted greenhouse gas after carbon dioxide. Despite lower emissions than those of carbon dioxide, methane receives significant attention owing to its more than 20-fold higher global warming potential. Consequently, the importance of research on methanotrophic bacteria, microorganisms capable of converting methane gas into high-value materials, is increasingly emphasized. In the case of methanotrophic bacteria, knowledge on episomal plasmids that can be used for genetic engineering remains lacking, which poses significant challenges to the engineering process. The replication origin sequences of natural plasmids within methanotrophic bacteria have been predicted through in silico methods. The basic characteristics of the replication origin, such as a high A/T ratio, repetitive sequences, and proximity to proteins related to replication, have been used as criteria for identifying the replication origin. As a result, a region with a sequence of 18 base pairs repeated eight times could be identified. The putative replication origin sequence thus identified generally takes the form of iterons, but it also possesses unique features such as the length of the gap between iterons and the repetition of identical iteron sequences. This information can be valuable for future design of episomal plasmids applicable to methanotrophs.

DTN Routing with Back-Pressure based Replica Distribution

  • Jiao, Zhenzhen;Tian, Rui;Zhang, Baoxian;Li, Cheng
    • Journal of Communications and Networks
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    • 제16권4호
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    • pp.378-384
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    • 2014
  • Replication routing can greatly improve the data delivery performance by enabling multiple replicas of the same packet to be transmitted towards its destination simultaneously. It has been studied extensively recently and is now a widely accepted routing paradigm in delay tolerant networks (DTNs). However, in this field, the issue of how to maximize the utilization efficiency of limited replication quota in a resource-saving manner and therefore making replication routing to be more efficient in networks with limited resources has not received enough attention. In this paper, we propose a DTN routing protocol with back-pressure based replica distribution. Our protocol models the replica distribution problem from a resource allocation perspective and it utilizes the idea of back-pressure algorithm, which can be used for providing efficient network resource allocation for replication quota assignment among encountered nodes. Simulation results demonstrate that the proposed protocol significantly outperforms existing replication routing protocols in terms of packet delay and delivery ratio.

도광판의 미세 패턴 사출 전사성이 LCD 광특성에 미치는 영향에 관한 해석적 연구 (Influence of Micro-Pattern Replication Ratio of Injection-Molded Light Guide Plate on Optical Aspect of LCD)

  • 홍주표;주병윤
    • 소성∙가공
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    • 제21권2호
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    • pp.126-130
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    • 2012
  • Accurate optical texturing of light guide plate over the entire surface area is an important technical issue in LCD TV industry. Injection molding process has the potential to produce large light guide plates having highly efficient optical textures such as micro-prism array. This study is focused on the effect of the degree of replication of the micro texture of the 40" injection molded light guide plates on the overall optical performance of the display panel. Measured replication ratios of the micro-textures formed with three different types of injection molding process were considered in the modeling of prismatic micro segment array. Optical simulation was conducted and results were discussed.

Recent Advances with the 3M PDP High Resolution Rib Replication Process

  • McGuire, P.;Cha, J.C.;Kikuchi, H.;Yoda, A.
    • 한국정보디스플레이학회:학술대회논문집
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    • 한국정보디스플레이학회 2007년도 7th International Meeting on Information Display 제7권1호
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    • pp.313-315
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    • 2007
  • 3M has developed a Precision Replication Process for the production of PDP Barrier Ribs and other display devices. This process, is fast, clean and capable of producing very high resolution (FHD and beyond), high aperture ratio and fine cell structures. Recent advances in materials and process features will be discussed.

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UV 나노 엠보싱 공정을 이용한 고종횡비 고분자 나노 섬모 어레이 제작 (Manufacture of High-Aspect-Ratio Polymer Nano-Hair Arrays by UV Nano Embossing Process)

  • 김동성;이현섭;이정현;이건홍;권태헌
    • 대한기계학회논문집A
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    • 제30권7호
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    • pp.773-778
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    • 2006
  • High-aspect-ratio nano-hair or nano-pillar arrays have great potential in a variety of applications. In this study, we present a simple and cost-effective replication method of high-aspect-ratio polymer nano-hair arrays. Highly ordered nano-porous AAO (anodic aluminum oxide) template was utilized as a reusable nano-mold insert. The AAO nano-mold insert fabricated by the two-step anodization process in this study had close- packed straight nano-pores, which enabled us to replicate densely arranged nano-hairs. The diameter, depth and pore spacing of the nano-pores in the fabricated AAO nano-mold insert were about 200nm, $1{\mu}m$ and 450nm, respectively. For the replication of polymer nano-hair arrays, a UV nano embossing process was applied as a mass production method. The UV nano embossing machine was developed by our group for the purpose of replicating nano-structures by means of non-transparent nano-mold inserts. Densely arranged high-aspect-ratio nano-hair arrays have been successfully manufactured by means of the UV nano embossing process with the AAO nano-mold insert under the optimum processing condition.

LIGA 공정을 이용한 평면형 광소자용 Ni 마스터 제작 (Fabrication of Ni master for the replication of planar optical devices by LIGA process)

  • 김진태;정명영
    • 대한기계학회:학술대회논문집
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    • 대한기계학회 2003년도 춘계학술대회
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    • pp.945-949
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    • 2003
  • LIGA(Lithographie Galvanoformung Abformung), a fabrication method for the production of microstructrues with a high aspect ratio, is now playing an important role in a fabrication of polymeric optical waveguide device as the replication processes have been developed such as hot embossing and injection molding. The present report deals with the fabrication of Ni master used for the replication of multi-mode polymeric optical waveguide. With the basic technological features in the sequence of the LIGA technique, we fabricated Ni master with 12 channel microstructures of $100\;{\times}\;100{\mu}m\;^2{\times}\;60mm$, and achieved an accuracy of ${\pm}1\;{\mu}m$. Manufactured polymeric optical wavegude with the same using hot embossing process has also the same accuracy and approved its mass production capability.

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Anodic Aluminum Oxide 기반 니켈 스탬퍼를 이용한 나노패턴 성형에 관한 연구 (A Study on the Fabrication of Nano Pattern using a Nickel Stamper Replicated from Anodic Aluminum Oxide)

  • 김신;김종선;홍석관;김현종;윤경환;강정진
    • 소성∙가공
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    • 제20권1호
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    • pp.23-28
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    • 2011
  • For the fabrication of nano patterned products manufacturing a nano patterned mold is needed in advance. The nano patterned stamper was fabricated by electroforming the AAO master with nickel. The surface of nickel-plated stamper had nano-patterned holes with the diameter of 73 nm and the depth of 83 nm. Hot embossing was used for forming P3HT sheet and the process factors of hot embossing were closer as pressure, temperature and time. In the present paper hot embossing experiments were performed to find the main process conditions to affect the replication ratio of nano patterns on surface of P3HT sheet. As a result, main contributing factors for the replication ratio of hot embossed pattern could be sequentially enumerated as pressure, temperature and time.

초음파 임프린팅에서 금형온도에 따른 미세패턴의 전사특성 연구 (Replication Characteristics of Micropatterns According to Mold Temperature in Ultrasonic Imprinting)

  • 민경빈;박종한;박창용;박근
    • 대한기계학회논문집A
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    • 제38권1호
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    • pp.51-57
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    • 2014
  • 초음파 임프린팅은 열가소성 고분자 기판에 미세패턴을 복제할 수 있는 공정으로 타 성형방법에 비해 에너지소모가 적고 성형시간이 단축되는 장점이 있다. 초음파 임프린팅 공정에서는 고분자 기판의 표면에 초음파 진동에너지를 인가하여 소재간의 마찰열과 미세하게 반복되는 변형에너지의 축적을 통해 고분자 표면을 국부적으로 가소화시켜 미세패턴이 전사된다. 본 연구에서는 초음파 임프린팅에서 금형 온도가 미세패턴의 전사성에 미치는 영향을 분석하였다. 이를 위해 금형온도를 변화시켜가며 임프린팅을 수행하여 미세패턴 성형 영역에서의 온도변화를 관찰하였고, 상기 온도변화를 고려하여 미세패턴의 충진과정을 전산모사를 통해 고찰하였다. 또한 금형온도 변화에 따른 패턴의 전사율 및 전사균일도를 측정하여 비교하였다. 상기 결과를 통해 금형온도를 높일수록 초음파 임프린팅시 미세패턴의 전사특성이 향상됨을 확인할 수 있었다.

LIGA-like 공정을 이용한 마이크로 부품 복제용 Ni과 Ni-W 금형 제조 및 특성 (Fabrication and Properties of Ni and Ni-W Electroplated Molds Using LIGA-like Process for Replication of Micro Components)

  • 황완식;박준식;강영철;조진우;박순섭;이인규;강성군
    • 한국재료학회지
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    • 제13권1호
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    • pp.6-10
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    • 2003
  • Electroplated Ni and Ni-W micro-molds using LIGA-like process for replication of micro-components such as microfluidic parts and micro optical parts have been investigated. In general, it is hard to produce micro-parts using conventional mechanical processes. Micro-mold formed by LIGA-like process could fabricate micro-parts with high aspect ratio. In this paper, fabrication and properties of electroplated Ni molds with varying applied current types as well as those of Ni-W molds were investigated. Ni molds fabricated under pulse-reverse current showed the highest hardness value of about 160 Hv. Ni-W molds showed the hardness of about 500 Hv which was much harder than that of Ni electroplated molds. The above results suggested that high quality micro-molds could be fabricated by using Ni electroplating of pulse-reverse type for core molds and sequential Ni-W alloys coating.