• 제목/요약/키워드: Replication quality

검색결과 111건 처리시간 0.024초

벼 형질전환계통의 미질특성에 대한 고찰 (Studies on the Grain Quality Characteristics of Rice Transgenic Lines)

  • 정종민;정지웅;강경호;이상복;모영준;김정곤;김경민;손재근
    • 한국작물학회지
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    • 제58권2호
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    • pp.203-211
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    • 2013
  • 국내 대학과 국립식량과학원 간의 협력 연구를 통해 확보된 32개 벼 형질전환 계통들 중 작물학적 성능이 유망한 11개 계통들에 대해 작물학적 특성과 미질 특성이 평가되었다. 주요 결과는 아래와 같다. 1. 이들 계통들은 수량성, 환경스트레스저항성, 내병성에 관여하는 7종의 유전자가 니폰바레, 낙동벼, 동진벼 등에 형질전환 되어 육성된 계통들이다. 2. 미질 특성과 관련된 11개 조사형질을 이용한 다변량 분석결과, 형질전환에 이용된 모품종과 도입유전자들에 대한 각 형질전환 계통군들의 뚜렷한 집구현상은 관찰되지 않았다. 3. 실용적 측면에서는 작물학적 특성이 모품종과 유사하면서 미질 특성이 양호한 계통이 다수 확인되었다. 4. 그러나 모품종의 유용한 작물학적 특성, 즉 '상품성'을 온전히 견지하고 있다고 판단되는 계통은 선정하기 어려웠다. 5. 도입할 유전자의 선정과 유전적으로 고정된 형질전환 계통을 확보하는데 소요되는 경비와 시간을 고려할 때, 향후 우량 벼 형질전환 계통의 육성효율을 제고하기 위해서는 기존에 비해 보다 구체적이며 차별화 된 육종전략의 개발이 필요하다고 판단되었다.

Effect of Substitution of Fermented King Oyster Mushroom By-Products Diet on Pork Quality during Storage

  • Chu, Gyo-Moon;Kang, Suk-Nam;Kim, Hoi-Yun;Ha, Ji-Hee;Kim, Jong-Hyun;Jung, Min-Seob;Ha, Jang-Woo;Lee, Sung-Dae;Jin, Sang-Keun;Kim, Il-Suk;Shin, Dae-Keun;Song, Young-Min
    • 한국축산식품학회지
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    • 제32권2호
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    • pp.133-141
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    • 2012
  • This study was carried out to investigate the effects of substitution of fermented king oyster mushroom (P. eryngii) by-products diet on pork meat quality characteristics, during the storage. A mixture of 40% king oyster mushroom by-products, 28% soybean meal and 20% corn was fermented for 10 d, and the basal diet was then substituted by the fermented diet mixture of up to 20, 50 and 80%, respectively. A total of 96 pigs were fed experimental diet (8 pigs per pen ${\times}$ 4 diets ${\times}$ 3 replication), and eight longissiumus (LD) per treatment were collected, when each swine reached to 110 kg of body weight. The Warner-Bratzler shear forces and cooking loss were significantly lowered in the treatments, while crude protein content and water holding capacity significantly (p<0.05) increased in the treatments than in the control group. The volatile basic nitrogen (VBN), at 1 d of storage, was lower in the treatments, while texture profiles and sensory evaluation did not differ between the control and the treatments (p>0.05). The pH, thiobarbituric acid reactive substances (TBARS), VBN and meat color in all treatments were increased as storage increased. Fermented king oyster mushroom by-products diet effects on lightness (CIE $L^*$), yellowness (CIE $b^*$) and chroma were determined, when LD muscles in T2 and T3 treatments were higher (p<0.05), up to 7 d (p<0.05). Therefore, the results indicate that the substitution of the fermented king oyster mushroom by-products diet to swine diet influenced the quality of the meat and it may be an economically valuable ingredient.

산란성계에서 사료 급이량 조절이 생산성과 계육품질에 미치는 영향 (Effects of Regulate in Feed Intakes on Performance and Meat Quality in Old Laying Hens)

  • 강환구;김찬호
    • 한국가금학회지
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    • 제42권3호
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    • pp.205-214
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    • 2015
  • 본 연구는 100주령 이상의 산란성계에서 사료 급이량 조절에 따른 생산성, 도체 수율 및 육질을 주령에 따라 평가하고자 실시하였다. 공시 동물은 100주령 Hy-Line Brown 200수를 선별하여 A형 2단 케이지에 대조구 포함 총 5처리구로 구성하여 처리당 4반복, 반복당 10수씩(2수 수용 케이지 10개) 난괴법으로 임의 배치하였다. 시험 기간 동안 물은 자유 섭취하게 하였으며, 일반적인 점등관리(자연일조 + 조명; 16hr)를 실시하였다. 시험구는 대조구(일일 사료 섭취량 100%), 90%, 60%, 50%, 20% 총 5처리였다. 산란율은 사양 시험 기간 동안 일일 사료 섭취량이 감소함에 따라 산란율 및 사료 요구량은 유의적으로(P<0.05) 감소하였다. 도체 수율 역시 사양시험 기간 동안 일일 사료 섭취량이 감소함에 따라 도체 수율 및 부분육(가슴, 다리, 날개) 비율이 유의적으로(P<0.05) 감소하였다. Leukocyte 함량은 일일 사료 섭취량이 50%, 20%일 때 WBC, HE, LY, MO 및 EO 함량의 비율이 유의적으로(P<0.05) 높았다. 가슴육 내 일반 성분항목, 보수력, 가열감량 및 지방산 함량에 있어서는 사양시험 기간과 일일 사료 섭취량에 따른 유의적인 차이는 나타나지 않았다. 본 실험 결과 일일 사료 섭취량이 감소할수록 생산성과 도체율이 감소하였으나, 일일사료 섭취량을 60% 수준까지 낮춰도 무방하다고 생각되며, 계육 품질에 미치는 영향은 나타나지 않았다.

Effects of Green Tea Powder on Laying Performance and Egg Quality in Laying Hens

  • Uuganbayar, D.;Bae, I.H.;Choi, K.S.;Shin, I.S.;Firman, J.D.;Yang, C.J.
    • Asian-Australasian Journal of Animal Sciences
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    • 제18권12호
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    • pp.1769-1774
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    • 2005
  • This experiment was carried out to evaluate the effects of feeding green tea powder on laying performance and egg quality in hens. A total 180 'Tetran Brown' laying hens aged 40 weeks were assigned to 6 treatments in a completely randomized design. Each treatment consisted of five replicates accommodating six layers per replication. The experimental diets were a negative control containing no green tea, a positive control diet containing antibiotics (0.05% chlortetracycline) and diets containing 0.5%, 1.0%, 1.5% and 2.0% green tea powder. Egg production rate of layers fed the diets containing green tea powder did not differ significantly from that of the negative and positive controls (p>0.05). Egg weight was decreased significantly in the group fed the diet containing 0.5% green tea powder (p<0.05). Feed intake of layers was significantly higher for the diet containing 1.5% green tea powder compared to that of negative and positive control diets (p<0.05). The eggshell thickness reduced significantly in the layer group fed the diets containing green tea powder regardless of dietary levels (p<0.05). Green tea powder tended to reduce egg yolk cholesterol in this experiment. Particularly, dietary 2% level of green tea powder significantly suppressed the cholesterol contents of the egg yolk (p<0.05). Thiobarbituric acid value (TBA) of egg yolk was significantly reduced by green tea diets (p<0.05). The yellowness of egg yolk was increased in the layers fed the 2.0% green tea diet compared with that of control diet (p<0.005). The Linoleic and $\alpha$-linolenic acids tended to increase in the group fed diets containing 1.5% green tea powder even though there were no significant differences among treatments (p>0.05). The oleic and docosahexaenoic acid contents of the egg yolk were similar among treatments (p>0.05). Based on the results of the experiment, it is concluded that green tea powder inclusion in the diet for layers at 2.0% level can reduce the cholesterol content and TBA value of the egg yolk, implying its potential effect on egg quality parameters.

산란생산성과 인 이용성에 대한 Microbial Phytase의 첨가 효과 II. 무기태인 수주닝 다르고 칼슘수준이 높은 사료에 Microbial Phytase 첨가가 산란성 및 인 이용성에 미치는 영향 (Efficacy of Supplemental Microbial Phytase on Laying Performance and Phosphorus Utilization II. Effect of Microbial Phytase at Different Phosphorus Levels and High Calcium Content on Laying Performance and Phosphorus Utilization)

  • 김상호;유동조;이상진;강보석;서옥석;최철환;이원준;류경선
    • 한국가금학회지
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    • 제27권1호
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    • pp.25-30
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    • 2000
  • Present study was conducted to investigate effects of microbial phytase in laying hen diets on utilization of non-phytate phosphorus(NPP) whose levels were adjusted to be adequate or lower than that of NRC requirements. Birds of control roup were fed a diet containing 0.275% NPP and 3.4% Ca, satisfying the NRC(1994) feeding standard. bird on T1, T2 and T3 were allowed to eat diets containing NPP at 100, 80 and 60%, respectively, of Control group, and 4.0% Ca level along with a microbial phytase added at a level of 300 DPU. Three hundred and sixty, ISA Brown layers, 23-week-old, divided into four treatment groups with three replications per treatment and 30 layers per replication were fed the diets for 12 weeks. Levels of feed intake were not different among the groups, The egg mass/feed intake ratio appeared better in T2 group by about 8%, though without a statistical significance, compared to that of control. Egg production fate tended to be improved over the control group by feeding the 100%(T1) and 80%(T2) NPP diets added with phytase, with a significant difference for T2(p<0.05). Mean egg weight and egg shell quality, measured by breaking strength and thickness of the egg shell, of the T2 group tended to show numerically better, without a significance than those of control. Furthermore, birds of the T2 group showed higher calcium and phosphorus contents in tibia by about 9%(p<0.05) than the control. Overall performances of birds in T1 appeared better than those of control, but tended to be lower than those of the T2. The birds in T3 performed similar to the those of the other dietary groups except the relatively low tibia calcium level. In conclusion, the results of this study suggest that supplementation of microbial phytase at a level of 300 DPU was effective to spare about 20% of NPP in laying hen diets without any adverse effects on production performances and bone quality.

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Comparison of the Effect of Green Tea By-product and Green Tea Probiotics on the Growth Performance, Meat Quality, and Immune Response of Finishing Pigs

  • Ko, S.Y.;Bae, I.H.;Yee, S.T.;Lee, S.S.;Uuganbayar, D.;Oh, J.I.;Yang, C.J.
    • Asian-Australasian Journal of Animal Sciences
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    • 제21권10호
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    • pp.1486-1494
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    • 2008
  • The objective of this experiment was to compare the effects of green tea by-product and green tea probiotics on the growth performance, meat quality and immune response of finishing pigs. A total of 72 crossbred "Landrace$\times$Yorkshire" finishing pigs with an average of 76 kg body weight were assigned to 4 dietary treatments in a completely randomized design. Each treatment had 3 replications with 6 pigs per replication. The four dietary treatments were control, antibiotics (control diet with 0.003% chlortetracycline added), and diets containing 0.5% green tea by-product or 0.5% green tea probiotic supplementation. Weight gain was increased in 0.5% green tea probiotics treatment compared to others, but there was no significant difference (p>0.05). The incorporation of 0.5% green tea probiotics to diets reduced the feed conversion ratio in finishing pigs (p>0.05). The incorporation of 0.5% green tea by-product into the pig diet reduced the crude protein and fat contents of the meat (p>0.05). Pigs fed diets containing 0.5% green tea probiotic supplementation had lowered meat TBA values compared to those fed 0.5% green tea by-product (p<0.05). The proliferation of spleen cells stimulated with Con A (concanavalin: 0.1, 0.3, and $1.0{\mu}g/ml$) significantly increased with 0.5% green tea by-product treatment compared to antibiotic treatment (p<0.05), but was significantly decreased in 0.5% green tea probiotics treatment compared to the antibiotic treatment (p<0.05). When stimulated with $1.0{\mu}g/ml$ Con A, splenocyte production of IL-6 from pigs treated with 0.5% green tea by-product or green tea probiotics was significantly increased compared to the antibiotic treatment group (p<0.05). Splenocyte production of TNF-${\alpha}$ after treatment with $1.0{\mu}g/ml$ Con A was significantly higher following 0.5% green tea probiotics treatment (p<0.05), while TNF-${\alpha}$ production after $10.0{\mu}g/ml$ LPS (lipopolysaccharide) was significantly higher in the 0.5% antibiotic treatment group (p<0.05).

Effect of Green Tea Probiotics on the Growth Performance, Meat Quality and Immune Response in Finishing Pigs

  • Ko, S.Y.;Yang, C.J.
    • Asian-Australasian Journal of Animal Sciences
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    • 제21권9호
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    • pp.1339-1347
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    • 2008
  • The objective of this study was to determine the effects of green tea probiotics on growth performance, meat quality and immune response in finishing pigs, and to assess the possibility of substituting green tea probiotics for antibiotics in diets of finishing pigs. This green tea probiotics is made by mixing green tea powder and excipients (defatted rice bran and wheat bran) and fermenting the mixture with beneficial bacteria. A total of 90 crossbreed "Landrace$\times$Yorkshire" finishing pigs with an average body weight of $72.5{\pm}2.5kg$ were assigned to 5 dietary treatments in a completely randomized design. Each treatment had 3 replications with 6 pigs per replication. The five dietary treatments were control, antibiotic (0.003% chlortetracycline added) and 0.1, 0.5 and 1.0% of green tea probiotics. There were no significant differences in final body weight, daily weight gain, daily feed intake and feed conversion ratio in the green tea probiotics and antibiotic treatments (p>0.05). Crude protein content was significantly increased in the 0.1 and 1.0% green tea probiotics treatment groups (p<0.05) and there was no significant difference in crude fat content of the meat among the treatments. The TBA value of meat was significantly lowered with 0.5 and 1.0% green tea probiotics treatments compared to that of controls and statistically similar to the antibiotic treatment after 3 weeks of storage (p<0.05). The growth of spleen cells stimulated with Con A (0.1 and $1.0{\mu}g/ml$) was significantly increased with 1.0% green tea probiotics treatment compared to that of the control treatment (p<0.05). The growth of spleen cells stimulated with LPS (1.0, 3.0 and $10{\mu}g/ml$) was significantly increased in the 0.5% green tea probiotics group compared to the antibiotic group (p<0.05). In Con A ($1.0{\mu}g/ml$) medium, IL-6 production of spleen cells was significantly increased with 1.0% green tea probiotics treatment compared to that of the control (p<0.05). In LPS ($10.0{\mu}g/ml$) medium, TNF-${\alpha}$ production of spleen cells increased significantly in all green tea probiotics treatment groups compared to that of the control (p<0.05). Finally it can be summarized that addition of green tea probiotic has a positive effect similar to antibiotic and 0.5% is the suitable dietary supplementation dose for finishing pig production.

남은 음식물과 녹차 부산물이 산란계의 산란성적과 계란품질에 미치는 영향 (Effects of Dried Leftover Food and Green Tea By-Product on Performance and Egg Quality in Laying Hens)

  • 우간바야르 담딘수렌;구민정;배인휴;양철주;선상수
    • 유기물자원화
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    • 제14권4호
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    • pp.121-131
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    • 2006
  • 본 연구는 산란계에서 남은 음식물과 녹차 부산물이 산란계의 산란성적과 계란품질에 미치는 영향을 연구하였다. 공시동물은 50주령 Tetran Brown종 산란계 210수를 7처리 5반복 (반복당 6수)으로 임의 배치하였다. 시험설계는 대조구와 옥수수 곡류와 대두박을 대용한 건조된 남은음식물 10, 20, 30 및 40% 첨가구 (10% DLF, 20% DLF, 30% DLF 및 40% DLF)와 1.0%의 녹차 부산물 첨가구 (1.0% GTB)와 30% DLF와 1.0% GTB를 첨가한 혼합 첨가구를 두어 사양실험을 8주 동안 실시하였다. 산란율은 대조구에 비하여 10, 20 및 40% DLF 처리구에서 유의적으로 증가하였다 (P<0.05). 난중은 대조구에 비하여 10% DLF 처리구에서 유의적으로 감소하였다 (P<0.05). 사료 섭취량은 대조구보다 20% DLF 처리구와 30% DLF와 1.0% GTB의 혼합 첨가구에서 높게 나타났다 (P<0.05). 사료 요구율은 대조구에 비하여 10% DLF 처리구와 1.0% GTB 처리구에서 유의적으로 감소하였다 (P<0.05). 난황 콜레스테롤은 DLF 처리구와 대조구 사이에서 변화가 없었다 (P>0.05). 난황의 리놀렌산은 DLF 처리구와 1.0% GTB 첨가한 처리구에서 유의적으로 증가하였다.

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Effect of Sea Tangle (Laminaria japonica) and Charcoal Supplementation as Alternatives to Antibiotics on Growth Performance and Meat Quality of Ducks

  • Islam, M.M.;Ahmed, S.T.;Kim, Y.J.;Mun, H.S.;Kim, Y.J.;Yang, C.J.
    • Asian-Australasian Journal of Animal Sciences
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    • 제27권2호
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    • pp.217-224
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    • 2014
  • A total of 150 growing ducks were assigned to five dietary treatments to study the effect of sea tangle and charcoal (STC) supplementation on growth performance and meat characteristics in a completely randomized design. There were six replicates and five ducklings in each replication. The five dietary treatments were control, antibiotic, and 0.1%, 0.5%, and 1% STC supplemented diets. No significant differences were found on ADG, ADFI, and gain:feed among treatments in different weeks. The overall (0 to 3 weeks) ADFI decreased in antibiotic treatment (p<0.05) whereas the gain:feed increased significantly upon 1.0% STC supplementation compared to control (p<0.05). No significant variation was found in meat chemical composition except crude fat content which was high in 1.0% STC dietary group (p<0.05). Meat cholesterol was reduced in 0.1% STC group (p<0.05) compared to other dose levels while serum cholesterol was unaffected. High density lipoprotein (HDL) content was high in 1.0% STC (p<0.05) and low density lipoprotein (LDL) was low in 0.1% and 1.0% STC dietary groups (p = 0.06). No significant effect was found on the thiobarbituric acid reactive substances (TBARS) of fresh meat, whereas the TBARS value of meat preserved for 1 week was reduced significantly in STC dietary groups (p<0.05). The 0.1% STC dietary group showed an increased myristic acid (p = 0.07) content whereas, the content of eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids increased in STC supplementation than antibiotic group (p<0.05). An increased concentration of omega-3 fatty acids and a reduced ratio of n-6/n-3 PUFA ratio was found upon 1.0% STC supplementation compared to antibiotic dietary group (p<0.05). Therefore, 1.0% STC dietary supplementation can be used as alternatives to antibiotics in duck production.

기업 전사 자료 설계에서 역정규화 중심 데이터 모델링의 부작용 (Negative Side Effects of Denormalization-Oriented Data Modeling in Enterprise-Wide Database Design)

  • 이혜경
    • 전자공학회논문지CI
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    • 제43권6호
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    • pp.17-25
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    • 2006
  • 전산화 대상 규모가 확대되면서 새로운 방식의 데이터 모델링 방법론이 필요하게 됨에 따라 자료 설계라는 것에 최근 들어 1980년대 초반 못지 않게 이른 바 데이터 거버넌스 혹은 데이터 질 등의 화두의 모습으로 큰 관심이 재연되고 있다. 그러나 데이터 모델링에 대한 확고한 견해를 가지고 있지 못하기 때문에 막상 데이터베이스 설계를 실무적으로 소화해 내기에는 적지 않은 어려움을 겪게 된다 본 논문에서는 업무의 요구 형성/분석 단계부터 데이터베이스 구현까지 최종 단계까지를 체계적인 방법론을 통해 정보시스템의 데이터 모델을 창출하는 정규화 중시의NS방법론을 적용한 설계방법과 기존의 관행에 의해 역정규화를 어쩔 도리 없이 용인하고 마는 정보공학(Information Engineering, 약칭 IE)방법론 기반의 설계방법과 비교한다. 동일한 업무를 대상으로 설계된 데이터 설계 산출물을 기준으로 각종 측면에서 비교해 봄으로써 정규화 중시 기반의 체계적 방법론이 기업의 정보시스템의 성능 향상에 어느 정도 기여하는지를 정량 파악하였다. 비교 실험 결과, 개체 설정의 타당성, 설계의 균형성 지표인 사각고리형 경로 유무, 그리고 속성중복률 면에서 모두 NS방법론적 설계가 우수하였다.