• Fisheries and Aquatic Sciences
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• 제21권8호
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• pp.29.1-29.8
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• 2018

This study was conducted to evaluate the use of tuna by-product meal (TBM) as a substitute for fishmeal in juvenile Korean rockfish (Sebastes schlegeli) diets. Five isonitrogenous (52% crude protein) and isocaloric (4.9 kcal/g) diets were prepared. Control diet (Con) was formulated to contain brown fishmeal, whereas 25, 50, 75, and 100% of fishmeal was substituted with the same percentage of TBM in the TBM25, TBM50, TBM75, and TBM100 diets, respectively. Three replicate groups of fish (initial weight, $29.5{\pm}0.6g$) were fed one of the five diets for 12 weeks. The mean weight gain of fish fed the Con and TBM25 diets was significantly higher than that of fish fed the TBM100 diet (P < 0.05), but it was not significantly different from the weight gain of fish fed the TBM50 and TBM75 diets. The feed efficiency and protein efficiency ratios of fish fed the Con diet were significantly higher than those of fish fed the TBM100 diet (P < 0.05), but were not significantly different from those fed the TBM25, TBM50, and TBM75 diets. The TBM25, TBM50, TBM75, and TBM100 diets had significantly (P < 0.05) lower incidence cost and higher profit index than the Con diet. These results suggest that the tuna by-product meal used in this study could replace up to 75% of fishmeal protein in the diet without reducing growth and feed utilization in juvenile Korean rockfish weighing 29-53 g. By considering feed cost, the TBM 75 diet is recommendable for Korean rockfish farming.

• Fisheries and Aquatic Sciences
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• 제18권2호
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• pp.159-164
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• 2015

A feeding experiment was conducted to determine the effect of dietary distillers dried grain (DDG) on the growth and body composition of juvenile abalone, Haliotis discus hannai. Three diets were formulated to contain 0% (DDG0), 30% DDG (DDG1) replacing wheat flour and 30% DDG (DDG2) replacing fish meal and wheat flour. A commercial feed (CF) was also compared with experimental diets. Three replicate groups of abalone averaging $0.6{\pm}0.01g$ were fed one of the four diets for 14 weeks. The highest survival rate was observed in the abalone fed the DDG2 diet, which was higher than that of abalone fed the DDG0 diet. Weight gain, shell length, shell width and soft body weight of the abalone were not affected by dietary DDG and CF diet (P > 0.05). Proximate and amino acid composition of the soft whole body were not affected by dietary DDG and CF diet (P > 0.05). The result of this experiment suggests that DDG is a good ingredient to replace fish meal and wheat flour in the diet and could be used up to 30% in the diet without negative effects on the growth performance of juvenile abalone Haliotis discus hannai.

• 한국수산과학회지
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• 제50권1호
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• pp.8-14
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• 2017

A feeding trial was conducted to evaluate the effect of dietary supplementation with fermented soybean meal by Bacillus subtilis (BFSBM) on growth performance, feed utilization, innate immunity, water quality, and disease resistance of Litopenaeus vannamei. A basal fish meal-based diet was regarded as a control and two other diets were prepared by replacing 30% fish meal with either soybean meal (SBM) or BFSBM. Triplicate groups of shrimp ($0.80{\pm}0.010g$) were fed one of the experimental diets for 6 weeks. No differences were found in final body weight, feed conversion ratio, or survival among all dietary treatments after the feeding trial. Shrimp fed the BFSBM diet had significantly higher phagocytic and phenoloxidase activities than those of shrimp fed the SBM diet. Total ammonia concentrations in culture water during a zero water exchange test tended to decrease in tanks where BFSBM diet was provided. Disease resistance of shrimp to Vibrio harveyi tended to increase in the BFSBM group during 14 days of challenge compared to that in the control (CON) and SBM groups. These results show that dietary supplementation with BFSBM can enhance the innate immunity of Pacific white shrimp and replace at least 30% of dietary fish meal.

• Asian-Australasian Journal of Animal Sciences
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• 제14권1호
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• pp.82-87
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• 2001

A feeding trial was conducted to evaluate the effects of replacing fish meal (FM) with ensiled shrimp by-product (ESB) in a cassava root meal and rice bran-based diet on the performance and carcass characteristics of growing pigs. Thirty six crossbred (Large White$\times$Mong Cai) pigs, with an average initial BW of 19.4 kg, were randomly allocated to one of three different dietary treatments in which the crude protein of the FM was replaced with 0, 50 or 100% ESB. The animals were fed restrictedly and at the end of the experiment at a BW of about 90 kg, 6 representative animals in each treatment group were slaughtered for carcass quality evaluation. Animal growth performance and daily feed intake were significantly reduced (p<0.05) by the inclusion of shrimp by-products in the diets, whereas feed conversion ratios and carcass measurements were not significantly affected (p>0.05). Daily weight gains of the pigs fed the 100% FM diet and 50% ESB diet were significantly (p<0.05) higher than those of pigs fed the 100% ESB diet. In conclusion, from an economical as well as performance point of view, ESB can replace 50% of the crude protein of FM in cassava root meal and rice bran-based diets for growing pigs with a low genetic growth potential.

• 한국수산과학회지
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• 제29권2호
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• pp.135-142
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• 1996

여러 가지 어분의 이용성을 검토하기 위해 어분의 종류와 혼합비를 달리한 실험사료로 2회에 걸쳐 사육 실험을 실시하였다. 단백질원으로 북양어분 간접식을 $100\%$ 첨가한 사료와 북양어분을 갈색어분으로 $100\%$ 대체한 2종류의 실험사료를 제조하여 평균체중 5.7g의 조피볼락을 대상으로 45일간 사육한 결과, 최종평균체중, 증중율 및 사료효율 모두 갈색어분 첨가구가 대조구보다 유의하게 낮은 값을 보인 반면 (P<0.05), 일간 사료섭취량, 단백질 및 지질축적율과 단백질 및 지질의 외견상 소화율은 대조구와 차이가 없었다. 단백질원으펀 북양어분 간접식 $100\%$ 첨가구 (대조구)와 북양어된 간접식을 북양어분 직접식으로 $50\%$ 및 $100\%$ 대체한 실험구, 갈색어분으로 $50\%$ 및 $100\%$ 대체한 실험구 및 어류가수분해단백질로 $50\%$ 대체한 실험구를 설정하여 평균체중 86.6g의 조피볼락을 21주간 사육한 결과, 사육기간에 따른 체중의 변화는 사료간에 거의 차이가 없이 직선적으로 증가하였으며, 최종평균체중, 증중율, 사료효율, 지질축적율은 어분종류에 따른 유의차가 인정되지 않았다(P>0.05). 단백질축적율은 갈색어분 $50\%$ 대체구가 유의적으로 높은 값을 보였으나(P<0.05), 모든 실험구의 값이 $22.4\~25.0\%$의 범위로 큰 차이는 없었다. 두 실험 모두 전어체의 단백질 및 지질 함량은 사료의 어분종류나 첨가비에 따른 차이는 없었다(P>0.05). 위의 결과들로 보아 성장효과가 어체 크기에 따라 달라짐을 알 수 있고, 성장함에 따라 어분 이용성의 선택 폭이 넓어지므로 어체 크기에 따라 배합사료의 주 단백질원을 적절히 조정하여 값비싼 북양 어분 간접식은 다른 값싼 어분으로 일부 또는 완전히 대체 가능할 것으로 판단된다.

• 한국수산과학회지
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• 제32권2호
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• pp.149-154
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• 1999

이 연구는 16주 동안 치어기 조피볼락 사료에 있어서 어분단백질에 대한 어분대체품의 이용 가능성을 결정하고자 실시하였다. 어분대체품 (FMA)은 혈분 (BM), 오징어간분 (SLP), 육골분(MBM), 수지박 (LM), 가금부산물 (PBP) 및 우모분 (FM)등으로 제작$\cdot$사용하였고, 필수아민노산 (EAA) 중 2가지 (Methionine& Lysine)를 첨가하여 사용하였다. 5가지의 실험사료는 조단백질 $50\%$로, 가용성 에너지 15.6KJ/g으로 조정하였다. 실험사료는 조단백질을 기준으로 어분단백질과 어분대체품 비율을 다음과 같이 요약하였다 :사료 1, $100\%$ WFM: 사료 2, $87.5\%$ WFM : $12.5\%$ FMA, 사료 3, $75\%$ WFM : $25\%$ FMA;사료 4, $75\%$ WFM : $25\%$ FMA+2 EAAs; 사료 5, $50\%$ WFM : $50\%$ FMA+2 EAAs (Methionine& Lysine). 증체율, 사료효율, 일간성장률 및 단백질 전환효율에 있어서 사료 2 ($12.5\%$ FMA)는 사료 1 (대조구)에 비해 유의적인 차이가 없었지만 (P>0.05), 나머지 사료구들은 대조구에 비해 유의적으로 낮게 나타났다 (P>0.05). 필수아미노산을 첨가한 사료 3 ($25\%$ FMA)과 사료 4($25\%$ FMA+2EAAs)의 두 사료구간에서는 사료구 4가 유의적으로 높은 경향을 보였다(P<0.05). 따라서, 치어기 조피볼락에 있어서 필수아미노산 첨가없이 어분단 백질을 복합 어분대체품 (FMA)으로 $12.5\%$ 정도 대체 가능함을 알 수 있었고, 2개의 필수아미노산의 첨가는 어분대체품에 사용되었을 경우에는 어느 정도 효과가 나타났다.

• 한국수산과학회지
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• 제31권3호
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• pp.380-385
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• 1998

본 연구는 성장기 잉어 사료의 어분 대체사료원으로서 축산가공혼합부산물 (Animal by-product mixture. ABPM)의 이용 가능성과 어분단백질 대체 범위를 결정하고 잉어용 어분대체품개발의 기초자료를 제공하고자 실시되었다. 본 실험사료의 동물성 단백질원으로는 북양어분(White Fish Meal, WFM)과 수지박 (Leather Meal. LM). 육골분 (Meat&Bone Meal, MBM). 혈분 (Blood Meal, BM). 오징어내 장분 (Squid Liver Powder, SLP)의 혼합물인 축산가공부산혼합물 (Animal by-product mixture. ABPM)을 사용하였고. 식물성 단백질원으로는 대두박 (Soybean Meal, SM)과 콘글루텐밀 (Corn Gluten Meal, CGM)을 사용하였다. 모든 실험사료는 조단백질함량 $40\%$. 가용성 에너지 15.3 KJ/g (protein. carbohyd-rate and lipid; 16.7, 16.7 and 37.7 J/g)으로 동일하게 맞추었고 각 사료의 성분조성은 다음과 같이 요약된다. diet 1, $100\%$ WFM+$0\%$ ABPM ($0\%$ ABPM. control); dict 2, $75\%$ WFM+$25\%$ ABPM ($25\%$ ABPM); diet 3, $50\%$ WFM+$50\%$ ABPM ($50\%$ ABPM): diet 4, $25\%$ WFM+$75\%$ ABPM ($75\%$ ABPM), diet 5, $0\%$ WFM+$100\%$ ABPM ($100\%$ ABPM). 각 사료내 단백질원으로서, 동물성 단백질은 어분과 ABPM을 사용하여 총단백질의 $34.7\%$를, 식물성 단백질은 $65.3\%$를 공급하였다. 일주간의 먹이붙임후에 잉어는 각 실험군별 평균어체중 10g 되도록하여 3반복으로 무작위 배치하였고, 전반기 첫 번째와 두번째 4주간의 사육기간이 끝난 8주후에는 평균어체중이 28g 되도록 재배치하여 추가로 후반기 세번째 4주간 총 12주동안 사육하였다. 사료는 1일 3회 어체중의 $2.7\~4\%$로 12주간 공급하였다. 매 2주마다 체중을 측정하였고 전반기 8주, 12주후 체장, hemoglobin, hematocrit 및 일반성분 분석을 실시하였다. 전반기 첫 번째 4주후 증체율 및 사료전환효율은 유의적 차이가 없었으나 전반기 두번째 4주후 사료 3, 4 및 5는 사료 I (대조구)보다 유의적으로 낮았다 (P<0.05). 이와는 달리 후반기 세번째 4주후의 모든 사료구의 증체율 및 사료전환효율에는 유의적 차이가 없었다 (P>0.05). 또한, 전어체 일반성분 분석 결과 전$\cdot$후반기동안 모든 사료구의 일반성분치 (수분, 조단백, 조지방, 조회분)에는 유의적 차이가 없었다 (P>0.05). 본 실험 결과, 사료내 동물성 단백질원으로 ABPM은 충분한 먹이붙임 후에 어분단백질의 $100\%$까지 대체 가능함을 보여 주었다.

• 한국수산과학회지
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• 제43권4호
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• pp.314-319
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• 2010

This study determined the proper inclusion levels of squid liver powder (SLP) - soy protein concentrate (SPC) premix as a fish meal (FM) substitute in the diet for juvenile olive flounder (Paralichthys olivaceus). All the experimental diets were formulated to be isonitrogenous (50% crude protein) and isocaloric (17.1 kJ energy $g^{-1}$ diet). Five diets were formulated with SLP-(SPC) replacing 0, 25, 50, 75, and 100% of the FM (SLP $(SPC)_0$, SLP $(SPC)_25$, SLP $(SPC)_50$, SLP $(SPC)_75$, and SLP $(SPC)_100$, respectively). Triplicate groups of 12 fish averaging $5.56{\pm}0.10\;g$ ($mean{\pm}SD$) were fed one of the five experimental diets for 7 weeks. The weight gain (WG), specific growth rate (SGR), feed efficiency (FE), and protein efficiency ratio (PER) of fish fed SLP $(SPC)_0$ were significantly (P<0.05) higher than those of fish fed SLP $(SPC)_50$, SLP $(SPC)_75$ and SLP $(SPC)_100$, while those of fish fed SLP $(SPC)_0$ and SLP $(SPC)_25$ were similar. The whole-body crude protein content of fish fed SLP $(SPC)_0$ was significantly (P<0.05) lower than that of fish fed SLP $(SPC)_50$, SLP $(SPC)_75$, and SLP$(SPC)_100$, while those of fish fed SLP $(SPC)_0$ and SLP $(SPC)_25$ were similar. The whole-body crude lipid of fish fed SLP $(SPC)_0$ was significantly (P<0.05) lower than that of fish fed SLP $(SPC)_50$ and SLP $(SPC)_75$, while those of fish fed SLP $(SPC)_0$, SLP $(SPC)_25$ and SLP $(SPC)_100$ were similar. Based on the fish growth performance results obtained from this study, the SLP (SPC) premix can substitute up to 25% of the FM in the diets of juvenile olive flounder.

• Animal Bioscience
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• 제34권12호
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• pp.1951-1962
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• 2021

Objective: This study was conducted to evaluate single cell protein (SCP), produced from Methylococcus species, as a protein source on the growth performance, carcass traits and gut health of broiler chickens. Methods: Ten iso-nitrogenous and iso-caloric diets containing 0 (Control), 2.5%, 5%, 7.5%, and 10% SCP replacing either soybean meal (T₁ to T₅) or fish meal (T₆ to T₁₀) were formulated. Each diet prepared for starter (0 to 14 days), grower (15 to 21 days), and finisher (22 to 42 days) phases was offered to four replicates of 10 chicks each (n = 400). Growth performance at different phases and carcass characteristics and intestinal morphology on 42nd day of trial were measured. Results: Body weight gain in groups fed 2.5% and 5% SCP diets were comparable to control during different phases and cumulatively, however lower (p<0.01) in 7.5% and 10% SCP diets. Feed conversion ratio was better (p<0.01) in 2.5% and 5% SCP diets. Dressing percentage, abdominal fat percentage and meat:bone ratio were not affected (p>0.05) by SCP inclusion in the diets. However, breast percentage was higher (p<0.01) in 2.5% and 5% SCP groups and thigh percentage higher in 7.5% and 10% SCP groups. Total microbial count in duodenum, jejunum and ileum were not affected (p>0.05) by SCP inclusion up to 10% in diets. Duodenal villi length and crypt depth were highest (p<0.01) in group fed 5% SCP diets and lowest in group fed 10% SCP diets. Jejunal villi length and crypt depth as well as ileal villi length were lowest (p<0.01) in group fed 10% SCP diets. Body weight gain, feed consumption, feed conversion ratio and gut health were better (p<0.01) in broilers fed fish meal based diets compared to soybean meal based diets. Conclusion: It was concluded that inclusion of SCP up to 5% replacing soybean meal in broiler diets is beneficial in improving growth rate, breast yield and gut health status.

• Fisheries and Aquatic Sciences
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• 제22권11호
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• pp.25.1-25.9
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• 2019

Background: Due to the continuous demand for fish coupled with decline in capture fisheries, there is the need to increase aquaculture production to meet the demand. Aquaculture is faced with high cost of feeding since fish oil and fish meal are expensive. In view of this, there are calls to explore alternatives that are cheap and reliable. Objectives: This study on Oreochromis niloticus was conducted to evaluate the effects of replacing fish oil (FO) with palm oil (PO) at 0%, 25%, 50%, 75%, and 100% on muscle fatty acid and proximate composition as well as growthrelated enzyme activities and mRNA expression. Methods: Oreochromis niloticus were fed five experimental diets (33% crude protein and 10% crude lipid) for 8 weeks. Feed had variation in fish oil and palm oil contents. After the 8 weeks feeding trial, five fish were sampled from each tank (15 from each treatment) and euthanized using an excess dose of tricaine methane sulfonate (MS-222 at 200 mg/L). Fatty acid and enzyme activities were analyzed using standard protocols. Also, RT-qPCR was used to quantify the expression levels of selected growth-related genes. Results: Fish fed 25% PO recorded the least muscle protein content and was significantly lower than the group fed 100% PO. Paired box protein 7 (Pax-7) enzyme activity was significantly higher in the group fed 50% PO compared to the groups fed 25% PO and 100% PO, while caplain-3 (Capn-3) was significantly lower in the group fed 0% PO compared to all other groups. There was a significant difference among treatments with respect to mRNA expression of Pax-7 and Capn-3. Group fed 25% PO had significantly lower mRNA expression of Pax-7, while the group fed 75% PO recorded significantly higher mRNA expression of Capn-3 compared to groups fed 0% PO, 25% PO, and 100% PO. Pearson's correlation analysis revealed that Igf-I and Igf-II mRNA expression have significant correlation with n-3 polyunsaturated fatty acids content in muscle. Conclusion: The results suggest muscle protein content could be modified if FO is replaced with PO. Also, mRNA expression of Pax-7 and Capn-3 is affected by replacing FO with PO.