• Title/Summary/Keyword: Repeat System

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Combining In Silico Mapping and Arraying: an Approach to Identifying Common Candidate Genes for Submergence Tolerance and Resistance to Bacterial Leaf Blight in Rice

  • Kottapalli, Kameswara Rao;Satoh, Kouji;Rakwal, Randeep;Shibato, Junko;Doi, Koji;Nagata, Toshifumi;Kikuchi, Shoshi
    • Molecules and Cells
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    • v.24 no.3
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    • pp.394-408
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    • 2007
  • Several genes/QTLs governing resistance/tolerance to abiotic and biotic stresses have been reported and mapped in rice. A QTL for submergence tolerance was found to be co-located with a major QTL for broad-spectrum bacterial leaf blight (bs-blb) resistance on the long arm of chromosome 5 in indica cultivars FR13A and IET8585. Using the Nipponbare (japonica) and 93-11 (indica) genome sequences, we identified, in silico, candidate genes in the chromosomal region [Kottapalli et al. (2006)]. Transcriptional profiling of FR13A and IET8585 using a rice 22K oligo array validated the above findings. Based on in silico analysis and arraying we observed that both cultivars respond to the above stresses through a common signaling system involving protein kinases, adenosine mono phosphate kinase, leucine rich repeat, PDZ/DHR/GLGF, and response regulator receiver protein. The combined approaches suggest that transcription factor EREBP on long arm of chromosome 5 regulates both submergence tolerance and blb resistance. Pyruvate decarboxylase and alcohol dehydrogenase, co-located in the same region, are candidate downstream genes for submergence tolerance at the seedling stage, and t-snare for bs-blb resistance. We also detected up-regulation of novel defense/stress-related genes including those encoding fumaryl aceto acetate (FAA) hydrolase, scramblase, and galactose oxidase, in response to the imposed stresses.

A Study on Post-formal Spatial Expression in 21 st Century Fashion Design (21세기 패션디자인에 나타난 탈정형적 공간 표현에 관한 연구)

  • Yang, Hee-Young;Kim, So-Young
    • Journal of the Korean Society of Costume
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    • v.58 no.8
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    • pp.91-105
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    • 2008
  • Spatial multiplication phenomenon, expressed through de-constructive trend since the late of 20C, have got changed existing fixed and dis-variable space to smooth, fluid, and changeable space. Ex-form and fluidity of new spatial paradigm have been focused through lots of scientific theories studied between digital thought and indeterminacy and rankless nature phenomena. In 21C fashion, indeterminate and irregular form and space have been crested continuously, which could not explain simply according as the fluidity theory Different from the space crested for multi-function and multipurpose, this is revealed the characteristics of chaosmos that communizes the order and the disorder, deconstruction and creation. Ex-formal space of fashion have shown the relationship of de-centerizing, de-territorial, and do-structural phenomenon among different fashion elements. This paper intends to understand the concept of ex-form, and study expressive manners of ex-formal space of 21C fashion, and conclusion as follows. 1) Overlap changes single space of fashion to multi-layered space through the repeat system of pleats, origami, and folding. 2) Ex-gravity expressed in deviation of the gravity acting vertical direction, for example, twisting, curve, winding, portion. 3) Morphing is shown the change process from single fixed form to different complex form. 4) Blurring is expressed in re-combination and re-arrangement among elements of fashion. 5) Blob shows hybrid fashion space through the liberal compounding and separation of a lot of different elements.

Transposable Genetic Elements, the Mechanisms of Transposition, and Their Uses in Genetic Studies (게놈 내 전이성 인자와 그 이동기구 및 이용)

  • 한창열;한지학
    • Korean Journal of Plant Tissue Culture
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    • v.22 no.5
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    • pp.241-260
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    • 1995
  • Transposons, present in the genomes of all living organisms, are genetic element that can change positions, or transpose, within the genome. Most genomes contain several kinds of transposable elements and the molecular details of the mechanisms by which these transposons move have recently been uncovered in many families of transposable elements. Transposition is brought about by an enzyme known as transposaese encoded by the autonomous transposon itself, but, in the unautonomous transposon lacking the gene encoding the transposase, movement occurs only at the presence of the enzyme encoded by the autonomous one. There are two types of transposition events, conservative and replicative transposition. In the former the transposon moves without replication, both strands of the DNA moving together from one place to the other while in the latter the transposition frequently involves DNA replication, so one copy of transposon remains at its original site as another copy insole to a new site. The insertion of transposon into a gene can prevent it expression whereas excision from the gene may restore the ability of the gene to be expressed. There are marked similarities between transposons and certain viruses having single stranded Plus (+) RNA genomes. Retrotransposons, which differ from the ordinary transposons in that they transpose via an RNA-intermediate, behave much like retroviruses and have a structure of integrated retrovial DNA when they are inserted to a new target site. An insertional mutagenesis called transposon-tagging is now being used in a number of plant species to isolate genes involved in developmental and metabolic processes which have been proven difficult to approach by the traditional methods. Attempts to device a transposon-tagging system based on the maize Ac for use in heterologous species have been made by many research workers.

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DNA fingerprinting analysis for soybean (Glycine max) varieties in Korea using a core set of microsatellite marker (핵심 Microsatellite 마커를 이용한 한국 콩 품종에 대한 Fingerprinting 분석)

  • Kwon, Yong-Sham
    • Journal of Plant Biotechnology
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    • v.43 no.4
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    • pp.457-465
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    • 2016
  • Microsatellites are one of the most suitable markers for identification of variety, as they have the capability to discriminate between narrow genetic variations. The polymorphism level between 120 microsatellite primer pairs and 148 soybean varieties was investigated through the fluorescence based automatic detection system. A set of 16 primer pairs showed highly reproducible polymorphism in these varieties. A total of 204 alleles were detected using the 16 microsatellite markers. The number of alleles per locus ranged from 6 to 28, with an average of 12.75 alleles per locus. The average polymorphism information content (PIC) was 0.86, ranging from 0.75 to 0.95. The unweighted pair group method using the arithmetic averages (UPGMA) cluster analysis for 148 varieties were divided into five distinctive groups, reflecting the varietal types and pedigree information. All the varieties were perfectly discriminated by marker genotypes. These markers may be useful to complement a morphological assessment of candidate varieties in the DUS (distinctness, uniformity and stability) test, intervening of seed disputes relating to variety authentication, and testing of genetic purity in soybean varieties.

The Complex Surgical Management of the First Case of Severe Combined Immunodeficiency and Multiple Intestinal Atresias Surviving after the Fourth Year of Life

  • Guana, Riccardo;Garofano, Salvatore;Teruzzi, Elisabetta;Vinardi, Simona;Carbonaro, Giulia;Cerrina, Alessia;Morra, Isabella;Montin, Davide;Mussa, Alessandro;Schleef, Jurgen
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • v.17 no.4
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    • pp.257-262
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    • 2014
  • Severe combined immunodeficiency (SCID) is a life-threatening syndrome of recurrent infections and gastro-intestinal alterations due to severe compromise of T cells and B cells. Clinically, most patients present symptoms before the age of 3 months and without intervention SCID usually results in severe infections and death by the age of 2 years. Its association with intestinal anomalies as multiple intestinal atresias (MIA) is rare and worsens the prognosis, resulting lethal. We describe the case of a four year-old boy with SCID-MIA. He presented at birth with meconium peritonitis, multiple ileal atresias and underwent several intestinal resections. A targeted Sanger sequencing revealed a homozygous 4-bp deletion ($c.313{\Delta}TATC$; p.Y105fs) in tetratricopeptide repeat domain 7A (TTC7A). He experienced surgical procedures including resection and stricturoplasty. Despite parenteral nutrition-associated liver disease, the patient is surviving at the time of writing the report. Precocious immune system assessment, scrutiny of TTC7A mutations and prompt surgical procedures are crucial in the management.

An Analysis of Research Related to the Massage as the Nursing Intervention (간호중재로서의 마사지 적용연구 분석)

  • Ji, Eun-Sun;Cho, Kyoul-Ja
    • Korean Parent-Child Health Journal
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    • v.7 no.2
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    • pp.168-181
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    • 2004
  • Purpose: The purpose of this study was to identified patterns of studies and to analyze the nursing research related to the massage intervention in Korea and to improve direction of nursing research in massage intervention in Korea. Methods: The research studies related to the massage were selected from the Korean Nurses Academic Society Journal and dissertation which were conducted experimental research design the last 7 years in Korea. The total numbers of the studies were 58. Result: The study subjects were post operational state in 34.5%, healthy adult in 17.2% studies. The others dealt with infant, cancer, dementia, hypertension, preterm neonates, elderly. Types of massage were whole body in 31.1%, foot in 22.6%, hand in 17.2%, back in 13.8% studies. And Meridian massage in 17.2%. Length of massage was below 5 minutes in 17.2%, 6~10 minutes in 29.4% and 11~20 minutes in 32.8% studies. Frequency of massage was only 1 time in 25.9%, 2~5 times in 31.1% studies. Massage produced psychological and physiological effects. Positive psychologic effects were anxiety relief, pain relief, mood enhancement and fatigue relief. Positive physiologic effects on body systems were blood pressure, heart rate, edema, skin temperature, immune system, blood glucose, excretion, PMS syndrome and growth and development. Conclusion: As a result, effects of massage were almost positive. Therefore massage is useful alternative nursing intervention. But more attention research is needed to identify the objectivity instrument to measure and repeat research.

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Rheological Properties and Cure Kinetics of Cycloaliphatic/DGEBA Epoxy Blend System Initiated by Cationic Latent Curing Agent (잠재성 경화제를 이용한 Cycloaliphatic/DGEBA계 에폭시 블렌드 시스템의 유변학적 특성 및 경화 동력학)

  • 곽근호;박수진;이재락;김영근
    • The Korean Journal of Rheology
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    • v.10 no.4
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    • pp.227-233
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    • 1998
  • The effects of 1 mol% N-benzylpyrazinium hexafluoroantimonate(BPH) as a thermal latent initiator and blend compositions composed of cycloaliphatic and DGEBA epoxies were investigated in the rheological properties and cure kinetics. Latent properties were performed by measurement of the conversion as a function of reaction time using isothermal DSC at $150^{\circ}C$ and $50^{\circ}C$ Rheological properties of the blend systems were investigated in terms of isothermal experiments using a rheometer. The gelation time was obtained from the evaluation of storage modulus (G'), loss modulus (G") and damping factor (tan$\delta$)). Cross-linking activation energy ($E_c$) was also determined from the Arrhenius equation based on gel time and curing temperature. As a result, the gel time and cross-linking activation energy increased with increasing DGEBA composition. The cure activation energies ($E_a$) were obtained by Kissinger method using dynamic DSC thermograms. In this work, the cure activation energy decreased with increasing CAE concentration, which might be resulted from the short repeat units, simple side-groups and viscosity of reaction media.edia.

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Open Platform for Improvement of e-Health Accessibility (의료정보서비스 접근성 향상을 위한 개방형 플랫폼 구축방안)

  • Lee, Hyun-Jik;Kim, Yoon-Ho
    • Journal of Digital Contents Society
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    • v.18 no.7
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    • pp.1341-1346
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    • 2017
  • In this paper, we designed the open service platform based on integrated type of individual customized service and intelligent information technology with individual's complex attributes and requests. First, the data collection phase is proceed quickly and accurately to repeat extraction, transformation and loading. The generated data from extraction-transformation-loading process module is stored in the distributed data system. The data analysis phase is generated a variety of patterns that used the analysis algorithm in the field. The data processing phase is used distributed parallel processing to improve performance. The data providing should operate independently on device-specific management platform. It provides a type of the Open API.

The β Subunit of Heterotrimeric G Protein Interacts Directly with Kinesin Heavy Chains, Kinesin-I (Kinesin-I의 kinesin heavy chains과 직접 결합하는 heterotrimeric G protein의 β subunit의 규명)

  • Seog, Dae-Hyun
    • Journal of Life Science
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    • v.20 no.8
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    • pp.1166-1172
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    • 2010
  • Kinesin-I exists as a tetramer of two heavy chains (KHCs, also called KIF5s), which contain the amino (N)-terminal motor domain and carboxyl (C)-terminal domain, as well as two light chains (KLCs), which bind to the KIF5s (KIF5A, KIF5B and KIF5C) stalk region. To identify the interaction proteins for KIF5A, yeast two-hybrid screening was performed and a specific interaction with the ${\beta}$ subunit of heterotrimeric G proteins ($G{\beta}$) was found. $G{\beta}$ bound to the amino acid residues between 808 and 935 of KIF5A and to other KIF5 members in the yeast two-hybrid assay. The WD40 repeat motif of $G{\beta}$ was essential for interaction with KIF5A. In addition, these proteins showed specific interactions in the glutathione S-transferase (GST) pull-down assay. An antibody to KIF5s specifically co-immunoprecipitated KIF5s associated with heterotrimeric G proteins from mouse brain extracts. These results suggest that kinesin-I motor protein transports heteroterimeric G protein attachment vesicles along microtubules in the cell.

Medical Convergence Analysis of Complaint about Medical Service in an Affiliated Hospital (일 상급종합병원 의료서비스 불만족 내용의 의료 융합적 분석)

  • Kim, Jung-Suk;Eom, Ae-Hyun;Yu, Moon-Sook
    • Journal of the Korea Convergence Society
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    • v.7 no.5
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    • pp.117-125
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    • 2016
  • Implementation of patient feedback is considered as a critical part of effective and efficient management. This study is a retrospective survey after to ensure the medical convergence analysis of contents on customer dissatisfaction using medical services. The data were collected from dissatisfaction 319 case at a affiliated hospital. The result indicate that a repeat visit than the initial visit, outpatients were more dissatisfied than the inpatients. The type of dissatisfaction were more dissatisfied of the in communication and explanation of medical service, nursing service and procedure of administrative services. In the classification of disease the percentages of dissatisfaction in the digestive system and neoplasm were high in both groups(p<0.05). Based on the study to improve the quality of medical service. Prevent the recurrence of dissatisfaction and to establish a customer-oriented business strategy. Characterized by dissatisfaction factors and contents of each patient and should be structured to specific disease types of services through continuous medical convergence research.