• 대한교통학회지
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• 제32권5호
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• pp.554-566
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• 2014

VMS의 운영적인 제공 목표는 운전자들의 경로선택을 통한 효율적인 시스템을 운영하는 것이다. VMS 정보 제공문제를 포함한 교통문제들은 게임이론을 통해 모형화 될 수 있지만 대다수의 연구들은 게임이론을 통하여 동일한 정보를 제공받더라도 운전자의 반응이 다양하게 나타나는 점을 반영하지 못하였다. 본 연구는 VMS교통정보에 대한 운전자들의 정보에 대한 반응을 진화적 게임모형을 활용하여 분석하고자 하였다. 실제 소통정보 및 VMS 정보 제공이력을 기초로 VMS정보에 따른 운전자들의 행동특성을 진화 게임이론에 적용해보았다. 분석결과 운전자들의 경로선택 비율은 VMS정보를 통한 기대통행시간과 진입교통량에 따라 달라지는 보수에 의해 결정되었다. VMS 정보는 진화적 게임의 보수에 영향을 미친다. 운전자들이 최초 어떠한 비율로 경로를 선택하더라도, 주기가 지남에 따라 진화적으로 안정한 상태로 수렴될 수 있는 것을 확인하였다. 또한 VMS정보가 과도한 통행시간이나 과소 통행시간을 제공할 경우 진화적으로 안정화되지 못하여 혼란이 가중될 수 있는 것으로 분석되었다. 결론적으로 교통문제와 같이 다른 운전자의 전략을 정확히 예측할 수 없고, 운전자 집단 간의 반복, 경험에 의해 합리적인 정보판단을 수행하는 경우, 진화 게임이론을 통해 전략적인 VMS 정보를 제공할 수 있을 것이라 기대할 수 있을 것이다.

• Radiation Oncology Journal
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• 제3권1호
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• pp.41-50
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• 1985

한국에서는 식도암의 임상적 관찰과 치료성적이 아직까지는 광범위하게 연구 및 보고되지 않았지만, 소화기계통에서 발생하는 악성종양 중에서는 비교적 적지 않은 것으로 알려져 있다. 의학적 지식, 진단적 설비, 방사선 치료의 적용, 외과적 수술의 진보, 그리고 최근의 화학요법의 재발 등과 같은 괄목할만한 향상에도 불구하고 예후는 극히 불량하여 5년 생존율이 10%이하로서, 과거에 비하여 크게 향상되지 않은 것으로 보고되고 있다. 이에 저자들은 1970년 1월부터 1980년 12월까지 만 11년 동안 식도암으로 진단되어 연세 암 센터에서 근치적 목적으로 방사선 치료를 받았던 63예에 대한 철저한 추적조사를 통하여 후향성 분석을 시행한 바 다음과 같은 결과를 얻었다. 1. 전 63예의 식도암 환자의 85.2%인 52예는 조직병리학적 소견에서 상피성세포암(Epidermoid Carcinoma)으로 확진되었다. 그리고 $T_1$인 17예를 제외한 46예73%의 병변은 상당히 진행되였던 예들이였다. 2. 방사선 조사선량은 일일 일회 2Gys가 조사되였으며 총 조사선량 $50{\sim}74Gys$가 $5{\sim}8$주간에 조사되었다. 3. 전 63예중 28예에서 방사선 치료 후 1개월만에 식도조형상을 통하여 식도암에 대한 방사선치료 효과를 평가하여 본 결과, 완전관해는 7예였으며 나머지 15예는 부분적 관해, 그리고 6예는 변화가 없었다. 4. 전 63예의 3년, 5년 생존율은 11.8%, 8.8%였으며 그리고 $T_1$인 17예의 3년, 5년 생존율은 24.7%, 20.8%였다.

• Asian-Australasian Journal of Animal Sciences
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• 제24권7호
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• pp.1019-1025
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• 2011

Toll-like receptors (TLRs) play an important role in the recognition of invading pathogens and the modulation of innate immune responses in mammals. The TLR4 and TLR7 are well known to recognize the bacterial lipopolysaccharide (LPS) and single stranded (ssRNA) ligands, respectively and play important role in host defense against Gram-negative bacteria and ssRNA viruses. In the present study, coding exon fragments of these two TLRs were identified, cloned, sequenced and analyzed in terms of insertion-deletion polymorphism, within bovine TLRs 4 and 7, thereby facilitating future TLR signaling and association studies relevant to bovine innate immunity. Comparative sequence analysis of TLR 4 exons revealed that this gene is more variable, particularly the coding frame (E3P1), while other parts showed percent identity of 95.7% to 100% at nucleotide and amino acid level, respectivley with other Bos indicus and Bos taurus breeds from different parts of the world. In comparison to TLR4, sequence analysis of TLR7 showed more conservation among different B. indicus and B. taurus breeds, except single point mutation at 324 nucleotide position (AAA to AAM) altering a single amino acid at 108 position (K to X). Percent identity of TLR7 sequences (all 3 exons) was between 99.2% to 100% at nucleotide and amino acid level, when compared with available sequence database of B. indicus and B. taurus. Simple Modular Architecture Research Tool (SMART) analysis showed variations in the exon fragments located in the Leucine Rich Repeat (LRR) region, which is responsible for binding with the microbial associated molecular patterns and further, downstream signaling to initiate anti-microbial response. Considering importance of TLR polymorphism in terms of innate immunity, further research is warranted.

• 지질공학
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• 제2권2호
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• pp.155-165
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• 1992

본 연구는 한반도의 지진지체구조 특성에 부합되는 내진설계용 부지응답 스펙트럼 작성을 위한 유사지진 선정에 관한 연구이다. Seismic Moment Tensor를 이용 파형을 Inversion한 결과에 의하면 지진원 특성상, Kern Conty 지진(1952년 7월 21일, 리히터 규모 ML = 7.2)은 한반도 지진지체구조 특성에 부합되는 현실적으로 유사한 외국의 지진들 중의 하나로서, 선정된 Kern Conty 지진으로 부터 발생한 강지진동 자료를 전산처리하여 표준응답 스펙트럼과 일반적 특성을 비교하여 보는 일도 의미있는 일이라고 할 수 있다.

• 대한수의학회지
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• 제37권4호
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• pp.925-934
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• 1997

This study was directed at inducing the production of antibodies by immunizing heifers with bovine sperm antigen and on measuring the serum antibodies using indirect immunofluorescence assay(IFA) and agglutination test. The effect of antisperm antibodies on fertilizing capacity of bovine spermatozoa was evaluated. 1. Three heifers between 12- and 15- month old were immunized with bovine spermatozoa or phosphate-buffered saline. In heifers immunized with bovine spermatozoa serum IgG level was highest between 3 weeks and 5 weeks postimmunization detected by IFA. The antibody levels persisted through week 7 and slowly declined until week 20 and then antisperm antibodies were localized on spermatozoa. The fluorescent antisperm antibodies were detected at 2~20 weeks and at 6~9 weeks postinoculation on acrosome and tail, respectively. Among 21 sera from repeat breeder cows, only one cow has shown positive antisperm antibody response detected by IFA. 2. In spite of vital rate of bovine sperm after swim-up was not significantly affected by different concentration of antisperm antibodies in sera, the numbers of bovine sperm after swim-up were significantly reduced in proportion to the increased concentration of antibodies. Above 1/512 dilution of antibody neither influence on vital rate and numbers of bovine sperm nor sperm agglutination after swim-up. The study has also shown that the vital rate and number of sperm after swim-up and capacitation were also significantly reduced by the addition of antisperm antibodies. Although antisperm antibodies did not influence on the acrosome reaction rate of sperm during swim-up, did significantly reduce the sperm acrosome reaction rate after capacitation. The studies have resulted that the bovine antisperm antibodies can prevent the sperm motility by agglutination and block the capacitation and acrosome reaction of bovine sperm.

• The Korean Journal of Physiology and Pharmacology
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• 제1권1호
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• pp.19-25
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• 1997

The C-terminus ends of the second putative transmembrane domains of both $M_1$ and $M_2$ muscarinic receptors contain a triplet of amino acid residues consisting of leucine (L), tyrosine (Y) and threonine (T). This triplet is repeated as LYT-TYL in $M_1$ receptors at the interface between the second transmembrane domain and the first extracellular loop. Interestingly, however, it is repeated in a transposedfashion (LYT-LYT) in the sequence of $M_2$ receptors. In our previous work, we investigated the possible significance of this unique sequence diversity for determining the distinct differential receptor function at the two receptor subtypes. However, we found mutation of the LYTTYL sequence of $M_1$ receptors to the corresponding $M_2$ receptor LYTLYT sequence demonstrated markedly enhanced the stimulation of phosphoinositide (PI) hydrolysis by carbachol without a change in its coupling to increased cyclic AMP formation. In this work, thus, the enhanced stimulation of PI hydrolysis in the LYTLYT $M_1$ receptor mutant was further investigated. The stimulation of PI hydrolysis by carbachol was enhanced in the mutant $M_1$ receptor, and this change was not due to alterations in the rate of receptor desensitization or sequestration. The observed larger response to carbachol at mutant $M_1$ receptors was also not due to an artifact resulting from selection of CHO cells which express higher levels of G-proteins or phospholipase C. Our data suggest that although the LYTTYL sequence in $M_1$ muscarinic receptors is not involved in determining receptor pharmacology, mutation of the sequence enhanced the coupling of $M_1$ receptors to the stimulation of phospholipase C.

• 한국간호교육학회지
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• 제6권1호
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• pp.147-159
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• 2000

The purpose of this study was to investigate the performance of clinical competency in nursing graduates and clinical nurses. The total of 234 subjects returned the questionnaire with 95% of response rates. The subjects of the study constituted of 195 nursing graduates and 39 clinical nurses. Self report questionnaires were used to measure the clinical competence of nursing graduates and clinical nurses. This instrument had four dimensions of competency : client and health need, nursing process, professional role, and nursing interventions. The data were analyzed by utilizing SPSSWIN and the results were as follows. 1) The mean score of the nursing intervention dimension was the most with 3.82 compared to professional role dimension(3.06), nursing process(3.03), client and health need dimension(2.94) in nursing graduates. 2) The mean score of the nursing intervention dimension was the most with 3.04 compared to client and health need dimension(2.82), professional role dimension(2.81), nursing process(2.77) in clinical nurses. And all of these dimensions' scores were lower than the nursing graduates' scores. 3) The mean scores of nursing process (t=3.76, p<.001) and professional role dimensions(t=3.53, p<.001) in nursing graduates were significantly higher than clinical nurses' scores. Our suggestions based on the results of this study is : 1. It is recommended to repeat the same designed study in large sample of clinical nurses for further study.

• 한국공간구조학회논문집
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• 제6권1호
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• pp.101-109
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• 2006

내진 댐퍼 브레이스를 가진 강구조물은 브레이스가 지진입력에너지를 충분히 흡수함으로써 주요한 구성부재의 치명적인 피해를 현저하게 저감시키는 것이 가능하므로, 이 시스템 도입에 따른 거동특성 파악 및 적용성에 대한 연구가 활발히 진행되고 있다. 내진 댐퍼 브레이스를 가진 강구조물의 설계에 있어서는 구조물에 대한 브레이스의 강성비를 결정하여야 하며, 내진성능이 우수한 구조물을 설계하기 위해서는 강성비에 따른 구조물의 지진응답 특성을 파악할 필요가 있다. 본 연구에서는 소성설계에 기초하여 내진 댐퍼 브레이스의 수평 강성비에 따른 강구조물의 초기 부재단면를 설계하고, 지진응답해석을 수행하여 초기 부재단면 설계의 타당성 검토 및 동적거동 특성을 파악한다.

• BMB Reports
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• 제53권10호
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• pp.521-526
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• 2020

Endogenous retroviruses (ERVs) are retrotransposons present in various metazoan genomes and have been implicated in metazoan evolution as well as in nematodes and humans. The long terminal repeat (LTR) retrotransposons contain several regulatory sequences including promoters and enhancers that regulate endogenous gene expression and thereby control organismal development and response to environmental change. ERVs including the LTR retrotransposons constitute 8% of the human genome and less than 0.6% of the Caenorhabditis elegans (C. elegans) genome, a nematode genetic model system. To investigate the evolutionarily conserved mechanism behind the transcriptional activity of retrotransposons, we generated a transgenic worm model driving green fluorescent protein (GFP) expression using Human endogenous retroviruses (HERV)-K LTR as a promoter. The promoter activity of HERV-K LTR was robust and fluorescence was observed in various tissues throughout the developmental process. Interestingly, persistent GFP expression was specifically detected in the adult vulva muscle. Using deletion constructs, we found that the region from positions 675 to 868 containing the TATA box was necessary for promoter activity driving gene expression in the vulva. Interestingly, we found that the promoter activity of the LTR was dependent on che-1 transcription factor, a sensory neuron driver, and lin-15b, a negative regulator of RNAi and germline gene expression. These results suggest evolutionary conservation of the LTR retrotransposon activity in transcriptional regulation as well as the possibility of che-1 function in non-neuronal tissues.

• BMB Reports
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• 제46권9호
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• pp.454-459
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• 2013

LRRK2 (leucine-rich repeat kinase 2) has been identified as a gene corresponding to PARK8, an autosomal-dominant gene for familial Parkinson's disease (PD). LRRK2 pathogenic-specific mutants induce neurotoxicity and shorten neurites. To elucidate the mechanism underlying LRRK2 expression, we constructed the LRRK2-promoter-luciferase reporter and used it for promoter analysis. We found that the glucocorticoid receptor (GR) transactivated LRRK2 in a ligand-dependent manner. Using quantitative RT-PCR and Western analysis, we further showed that treatment with dexamethasone, a synthetic GR ligand, induced LRRK2 expression at both the transcriptional and translational levels, in dopaminergic MN9D cells. Dexamethasone treatment also increased expression of ${\alpha}$-synuclein, another PD causative gene, and enhanced transactivation of the ${\alpha}$-synuclein promoter-luciferase reporter. In addition, dexamethasone treatment to MN9D cells weakly induced cytotoxicity based on an LDH assay. Because glucocorticoid hormones are secreted in response to stress, our data suggest that stress might be a related factor in the pathogenesis of PD.