• 대한한의학회지
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• 제21권3호
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• pp.119-128
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• 2000

목적 : Rhabdomyolysis에 의해 유발된 급성 신부전시 나타나는 신장세뇨관 세포에서 물질이동의 저해가 단삼 추출액에 의해 방지될 수 있는 지를 조사하였다. 방법 : 토끼에 50％ glycerol을 10ml/kg씩 대퇴근육내 주사한 후 뇨와 혈액을 채취하여 신기능을 측정하고, 신피질 절편을 분리하여 실험하였다. 결과： 토끼에 50％ glycerol을 10ml/kg씩 대퇴근육내 주사한 결과 사구체여과율의 감소와 Na 배설분율의 증가가 나타남으로서 glycerol 주입이 rhabdomyolysis에 의해 급성신부전이 유발되었음을 보였다. Glycerol을 주사하기 전 7일 동안 단삼 추출액 (0.05%)을 0.3 g/kg씩 경구 투여한 결과 glycerol에 의해 유발된 사구체여파율의 감소와 Na 배설분율의 증가가 유의하게 방지되었다. glycerol만을 주사한 동물에서는 포도당과 인산의 요배설분율이 각각 현저하게 증가하였으나, 이러한 증가는 단삼 추출액에 의해 억제되었다. 급성신부전이 유발된 신장피질에서 분리한 brush-border membrane vescicles (BBMV)에서 포도당과 인산의 이동은 정상 신장과 비교하여 유의한 감소가 나타나고, microsomal fraction에서 측정한 Na＋-K+-ATPase 활성도 억제되었다. 이러한 억제현상은 단삼 추출액을 전처치한 결과 방지되었다. 급성신부전이 유발된 신장피질 절편에서 유기 음이온인 P-aminohippurate 이동과 유기 양이온인 tetraethylammonium의 이동이 억제되었고, 이러한 변화는 단삼 추출액에 의해 방지되었다. Rhabdomyolysis에 의해 유발된 포도당과 인산의 배설분율의 증가는 항산화제로 잘 알려진 DPPD 전처치로 방지되었다. 결론 ： Rhabdomyolysis에 의한 급성신부전의 유발 과정에 반응성 산소기가 중요한 역할을 할 가능성을 보이고 있고, 단삼 추출액 전처치는 Rhabdomyolysis에 의한 급성 신부전시 나타나는 근위세뇨관에서 물질의 재흡수 장애를 방지하고 있다. 단삼 추출액의 방지 효과는 항산화작용에 기인할 것으로 사료된다.

• Journal of Acupuncture Research
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• 제19권5호
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• pp.199-208
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• 2002

Objective : This study was undertaken to determine if Carthami Semen Aquacupunc- ture(CSA) exerts protective effect against alterations in membrane transport function rabbits with mercury chloride(HG)-induced acute renal failure. Methods : The administration of Hg at a subcutaneous single dose of 10 mg/kg caused a reduction in GFR and an increase in fractional Na excretion, indicating generation of acute renal failure. When CSA were given for 7 days prior to Hg administration, such changes were significantly attenuated. The fractional excretion of glucose and phosphate was increased in rabbits treated with Hg alone. Results : The increase in rabbits treated with Hg following CSA are significantly lower than that in animals treated with Hg alone. Uptakes of glucose and phosphate in purified isolated brush-border membrane and Na-K-ATPase activity in microsomal fraction were inhibited in rabbits treated with Hg alone. Such changes were prevented by CSA. Uptakes of organic ions, PAH and TEA, in renal cortical slices were inhibited by the administration of Hg, which was prevented by CSA. Exposure of renal cortical slices to Hg in vitro caused an increased LDH release and lipid peroxidation, which was significantly prevented by CSA extract. Conclusions : These results indicate that the administration of Hg causes impairment in reabsorption of solutes in the proximal tubule via the generation of reactive oxygen species. CSA provides the protection against the impairment in proximal reabsorption, and its effect may be resulted from its antioxidant effect.

• Journal of Acupuncture Research
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• 제18권3호
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• pp.114-122
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• 2001

Objective : The present study was carried out to determine if Juglandis Semen Aquacupuncture(JSA) exerts beneficial effect against the urine concentrating defect induced by glycerol injection in rabbits. Methods : In order to test the effect of JSA, rabbits were acupunctured with $0.5m{\ell}$ of 1%JSA for 7days at both sides of $Sh{\grave{e}}ns{\bar{u}}$(BL23) before the glycerol injection. The other animals were pretreated with an equal volume of saline instead of JSA. Results : The urine flow was reduced, but the urine osmolality was significantly lower than the basal period in glycerol-injected animals, indicating that glycerol causes the urine concentrating defect. The fractional excretion of Na and K was increased in glycerol-treated animals. The Free-water clearance and fractional water excretion(V/GFR) were increased in animals treated with glycerol. Conclusion : These results indicate that glycerol injection resulted an impairment in the urine concentrating ability in rabbits. Such changes were prevented by JSA. JSA may be used as a method to treat and prevent glycerol-induced acute renal failure.

• Korean Journal of Acupuncture
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• 제23권1호
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• pp.45-57
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• 2006

Objective : This study was undertaker to determine if Juglandis Semen herbal acupuncture (JSA) exerts protective effect against alterations in membrane transport function in rabbits with mercury-induced acute renal failure. Methods : Nephrotoxicity was induced by subcutaneous administration of Hg(a single dose of 10mg/kg) and JSA was performed at both sides of Shenshu($(BL_{23})$, Sinsu) for 7 days. Results: The administration of Hg at a subcutaneous single dose of 10 mg/kg caused a reduction in GFR to 12% of the basal value and an increase in fractional $Na^+$ excretion to 8.9-fold, indicating generation of acute renal failure. When JSA were given for 7 days prior to Hg administration, such changes were significantly attenuated. The fractional excretion of glucose and phosphate was increased to approximately 102- and 35-fold, respectively, in rabbits treated with Hg alone. The increase in rabbits treated with Hg following ISA are significantly lower than that in animals treated with Hg alone. Uptakes of glucose and phosphate in purified isolated brush-border membrane and $Na^+-K^+-ATPase$ activity in microsomal fraction were inhibited in rabbits treated with Hg alone, suggesting that impairment in proximal reabsorption of glucose and phosphate is resulted from a direct damage of membrane transport carriers and disruption of the normal $Na^+$ gradient. Such changes were prevented by JSA. Conclusion These results indicate that the administration of Hg causes impairment in reabsorption of solutes in the proximal tubule via the generation of reactive oxygen species. JSA provides the protection against the Hg-induced impairment in proximal reabsorption, and its effect may be resulted from its antioxidant action.

• 대한약학회:학술대회논문집
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• 대한약학회 2000년도 NEW STRATEGY FOR DRUG DEVELOPMENT IN POST-GENOMIC ERA(대한약학회)
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• pp.275.1-275.1
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• 2000

• 생명과학회지
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• 제27권8호
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• pp.974-974
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• 2017

• 대한수의학회지
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• 제34권2호
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• pp.339-347
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• 1994

This study was carried out to investigate the pathological changes with paraquat(1.1'-dimethyl-4.4'-dipyrildiylium dichloride) administered by intraperitoneally, orally, skin applied in mice, rats and rabbits. Results were obtained as follows; In 2 days after paraquat administration clinical signs anorexia, depression, tachypnea, and tachycardia, respiratory failure, coma and death were observed in mice, rats and rabbits. Compared toxicity of paraquat with mouse were observed toward to paraquat that resistance was strong than rats and rabbits. Also, mortality of skin application were found the low than intraperitoneal and high than oral administration. In the case of gross observation were appear lips moisture in orally administered rats and rabbits by skin application. Lung of all laboratory animals were observed congestion and haemorrhage, swelling or atrophy. In the case of microscopic findings were severe congestion and haemorrhage, interstitial pneumonia of lung. Congestion and haemorrhage of liver, congestion and haemorrhage, renal tubule epithelium necrosis of kidney were observed in mice, rats and rabbits. Skin application group of mice, rats and rabbits showed infiltration of inflammatory cells and folliculitis of epidermis and dermis. Also, in oral administration group showed congestion and haemorrhage, tachment, necrosis of alimentary tract mucosa.

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 1988년도 학술대회지
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• pp.43-46
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• 1988

홍삼 $S_I$-분획의 투여로 중크롬산에 의한 신장세포독성이 감소되었다. 토끼에 중크롬산을 투여하면 급성신부전이 유발되어 10일이내에 대부분이 죽었다. 반면 홍삼 $S_I$-분획을 투여하면 치사율이 상당히 낮아졌으며, 생리적 증상이 현저히 개선되었다. 홍삼 $S_I$-분획의 투여로 중크롬산에 의하여 생기는 glomerulotubular imbalance가 호전되었으며, 당원생성, 해당속도, 젖산 생성속도가 정상화 되었다. 홍삼 $S_I$-분획의 효과는 비타민 C, EDTA, 휴로세마이드에 비하여 월등하였다.

• 생약학회지
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• 제17권3호
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• pp.206-214
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• 1986

These studies were conducted an attempt to investigate effects of 'Paeryungtang' and 'Kamipaeryungtang' water extracts on diuretic, antipyretic, antiinflammatory and analgesic actions. The results of these studies were summarized as follows; Increase in urinary volume, urinary $Na^+$ excretions were significantly recognized in normal rat. Increase in urinary volume, urinary $Na^+\;and\;Cl^-$ excretions were significantly shown in rat with 2mg/kg $HgCl_2$-induced acute renal failure. Antipyretic, anti-inflammatory and analgesic effects of 'Paeryungtang' and 'Kamipaeryungtang' were recognized in mice, rats and rabbits.

• 대한의생명과학회지
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• 제10권4호
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• pp.341-346
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• 2004

Tumor necrosis factor-α (TNF-α) or its mRNA expression are increased in acute nephrosis of various types including ischemia/reperfusion injury. This study was undertaken to determine whether pentoxifylline (PTX), an inhibitor of TNF-α production, provides a protective effect against hypoxia-induced cell injury in rabbit renal cortical slices. To induce hypoxia-induced cell injury, renal cortical slices were exposed to 100% N₂ atmosphere. Control slices were exposed to 100% O₂ atmosphere. The cell injury was estimated by measuring lactate dehydrogenase (LDH) release and p-aminohippurate (PAH) uptake. Exposure of slices to hypoxia increased the LDH release in a time-dependent manner. However, when slices were exposed to hypoxia in the presence of PTX, the LDH release was decreased. The protective effect of PTX was dose-dependent over the concentrations of 0.05∼1 mM. Hypoxia did not increase lipid peroxidation, whereas an organic hydroperoxide t-butylhydroperoxide (tBHP) resulted in a significant increase in lipid peroxidation. PTX did not affect tBHP-induced lipid peroxidation. Hypoxia decreased PAH uptake, which was significantly attenuated by PTX and glycine. tBHP-induced inhibition of PAH uptake was not altered by PTX, although it was prevented by antioxidant deferoxarnine. The PAH uptake by slices in rabbits with ischemic acute renal failure was prevented by PTX pretreatment. These results suggest that PTX may exert a protective effect against hypoxia-induced cell injury and its effect may due to inhibition of the TNF-α production, but not by its antioxidant action.