• Applied Microscopy
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• 제27권4호
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• pp.373-389
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• 1997

Morphological difference of the renal glomerulus at different age groups have been studies in one week-old, five weeks-old, eight weeks-old, six months-old, twelve months-old, eighteen months-old, twenty-four months-old, and thirty months-old ICR mice. Pieces of the tissue taken from the renal corticies were fixed in 2.5% glutaraldehyde-1.5% paraformaldehyde solution (0.1 Millonig's phosphate buffer pH 7.3), and 1% osmium tetroxide solution (0.1 M Millonig's phosphate buffer, pH 7.3), and were embedded in araldite mixture. The ultrathin sections were stained with uranyl acetate and lead citrate solution, and were observed under a JEM 100CX-II electron microscope. The results were as follows: 1 In the one week-old mouse, thicknesses of the three layers of the glomeluar basal lamina (lamina densa, lamina rara interna and lamina rara externa) are similar, but in the five weeks-old mouse, thick lamina densa becomes a greater portion of the thickness of whole glomerular basal lamina. 2. No difference was noticed between thickness of the renal glomerular basal lamina of the five weeks-old mouse compare with that of the one week-old one, but basal lamina of the eight weeks-old one is thickened considerably and thicknesses were maintained through twelve months-old one. After eighteen months, the thickness of the glomerular basal lamina is increased remarkably. 3. After eighteen months, electron dense deposits within the basal lamina of the renal glomeruli are observed frequently. 4. Amount of the microfilaments in the mesangial cells and the mesangial matrices are increasing during aging. 5. The thicknesses of the basal laminae of the Bowman's capsule are increasing during aging. 6. After twenty four months, the proximal tubular cell-like parietal cells with well developed microvilli are observed frequently. From the above results, it was suggested that the renal glomerulus matures structurally in five weeks, and the function of the glomerulus is suppressed after eighteen months.

• 대한수의학회지
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• 제37권1호
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• pp.41-57
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• 1997

This study was carried out to investigate the effect of selenium on the adriamycininduced renal lesions in male Sprague Dawley rats. A total of 60 Sprague-Dawley male rats were divided into 2 control groups(C1: saline, C2: selenium) and 2 treatment groups(T1: adriamycin, T2: adriamycin+selenium). The rats of the C1 and T1 groups were given normal saline(0.15ml/rat), the rats of the C2 and T2 groups were given sodium selenite(0.5mg/kg) intraperitoneally three days a week for 4 weeks. The treatment groups were dosed intraperitoneally with adriamycin(2mg/kg/day) five days at the second week. Animals were sacrificed at the 1st week, 2nd week and 3rd week after dosing with adriamycin. The morphologic abnormalities of the glomeruli and tubules in the kidney of male rats were examined histopathologically and electron microscopically.The results obtained were as follows : The mean body weight of adriamycin dosed group was significantly decreased as compared with that of control group at 4th week(p<0.05). In adriamycin and selenium dosed group, the mean body weight was decreased until the end of 2nd week but gradually increased from 3rd to 4th week. The histopathological findings of the renal corpuscle in adriamycin dosed group were parietal epithelial cell proliferation, vacuolization of glomerulus, and thickened basement membrane of the parietal epithelium. Proximal convoluted tubules were significantly dilated and the lumens were filled with renal cast. These lesions were generally not very significant in the rats given adriamycin and selenium. The electron microscopical findings of the renal glomerulus in the adriamycin dosed group were focal loss and fusion of the pedicels of the podocyte, and some vacuoles in the cytoplasm of the podocytes. There were numerous cytoplasmic vacuoles in the proximal and distal convoluted tubular cells. However, these ultrastructural changes were not significantly observed in the renal tubules of the rats of adriamycin and selenium dosed group. These results suggest that selenium may act as an inhibitor of the renal lesions induced by adriamycin in male rats.

• Applied Microscopy
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• 제29권1호
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• pp.25-41
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• 1999

종양의 치료제로 많이 사용되고 있는 대표적인 항암제 가운데 대사길항제인 5-fluorouracil과 항암항생제인 mitomycin C가 콩팥토리에 미치는 영향을 미세구조적으로 비교 관찰하고자 이 실험을 시행하였다. Mitomycin C는 $LD_{50}$의 1/20 이하인 $400{\mu}g/kg$를, 5-fluorouracil는 $LD_{50}$의 1/4 정도인 60mg/kg를 격일 간격으로 계속 투여하였다. 각 항암제투여군은 처음 주사후 4일 (2회 투여)과 7일 (3회 투여) 째에 희생시킨 후 콩괄겉질부위의 조직을 메어 JEM 100CX-II 전자 현미경으로 관찰하였다. 1. 5-fluorouracil 및 mitomycin C 투여후 4일군에서는 다같이 혈관사이막이 증가하여 모세혈관과 주머니공간이 매우 좁아 보이는 부분이 많았는데, 그 현상은 mitomycin C 투여군에서 더 심했다. 2. 5-fluorouracil 투여 7일군은 토리의 미세구조가 거의 정상군의 모습과 비슷하게 회복되어 보였으나 mitomycin C 투여 7일군은 토리의 미세구조가 정상군의 모습과 비슷하게 회복된 모습을 거의 관찰할 수 없었다. 3. Mitomycin C 투여 4일군과 7일군에서는 내피세포의 종창현상(swelling) 이 심해서 내강으로 돌출되었을 뿐만 아니라 혈관사이세포가 모세혈관내강으로 돌출되어 내강을 거의 막고 있는 경우가 자주 관찰되었다. 4. Mitomycin C 투여 4일군에서는 토리모세혈관바닥막의 속투명판과 바깥투명판의 전자밀도가 높아서 치밀판과의 경계가 뚜렷하지 못한 부분이 많았는데, 부위에 따라서는 속투명판이 넓어진 부분이 관찰되기도 하였다. 이상의 결과를 종합해 보면 mitomycin C를 투여하였을 경우에는 마우스의 콩팥토리는 모세혈관의 내강이 좁아지고 혈관사이막이 증가하며 내피세포는 종창현상을 보이는 등의 미세구조적 변화를 보였다. 그러나 5-fluorouracil을 투여하였을 경우, 초기 (4일)에는 발세포와 혈관사이막의 경우 mitomycin C 투여군에서와 비슷한 미세구조적 변화를 보였으나, 7일군에서는 거의 정상군의 소견과 비슷한 모습을 보였다. 이와 같은 결과는 콩팥토리를 비롯한 콩팥조직에 미치는 세포독성은 mitomycin C가 5-fluorouracil에 비하여 훨씬 더 강하기 때문이라고 생각된다.