• Journal of the korean academy of Pediatric Dentistry
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• v.34 no.2
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• pp.183-191
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• 2007

Through out the world dental caries seems to be decreased as it is difficult to make an accurate diagnosis for dental caries. The traditional diagnostic method which is probing and x-ray taking has many limitations to diagnose the early caries, so there were recommendations for the needs of new equipments such as laser fluorescence(LF), digital imaging fiber-optic trans-illumination(DIFOTI), and quantitative light fluorescence (QLF) which were developed from various study results. Also confocal laser scanning microscopy(CLSM) and ultrasonics are used for research progression. This study is to evaluate whether it is possible to monitor accurately for remineralization amount of enamel surface early caries using DIFOTI or LF After inducing artificial caries to bovine teeth to 10 participants remineralization was enhanced by 0 ppm and 500 ppm fluoride mouth rinse solution for 3 weeks. Then they were cross sectioned and analyzed using gold standard of the lesion depth measured by CLSM. The following results were obtained: 1. The measured percentage of light intensity(luminosity ratio) by DIFOTI increased with remineralization period, and showed significant reverse correlation with lesion depth measured by CLSM (p<0.01). 2. The measurement of laser fluorescence increased with remineralization period, and showed significant correlation with lesion depth measured by CLSM (p<0.01). 3. To the result for CLSM, 500 ppm fluoride mouth rinse group showed rapid rate for decreased tendency of lesion depth than 0 ppm fluoride mouth rinse group. In conclusion DIFOTI system was used to measure accurately for the remineralization amount of early surface caries, it is a very useful equipment to detect precisely the changes for early enamel caries remineralization during treatments.

• Restorative Dentistry and Endodontics
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• v.35 no.2
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• pp.96-105
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• 2010

The purpose of this study is to observe and compare the remineralization tendencies of artificial enamel caries lesion by remineralization solutions of different degree of saturations at pH 5.5, using a polarizing microscope and computer programs (Photoshop, Image pro plus, Scion Image, Excel). For this study, 48 sound permanent teeth with no signs of demineralization, cracks, or dental restorations were used. The specimens were immersed in lactic acid demineralization solution for 2 days in order to produce artificial dental caries that consist of surface and subsurface lesions. Each of 9 or 10 specimens was immersed in pH 5.5 lactic acid buffering remineralization solution of four different degrees of saturation (0.507, 0.394, 0.301, and 0.251) for 12 days. After the demineralization and remineralization, images were taken by a polarizing microscope (${\times}100$). The results were obtained by observing images of the specimens, and using computer programs, the density of caries lesions were est mated. While the group with the lowest degree of saturation (0.251) showed total remineralization feature from the surface to the subsurface of the lesion, the group with the highest degree of saturation (0.507) showed demineralization mainly on the surface of the lesion at the constant organic acid concentration 0.01 M and pH 5.5.

• Proceedings of the Korea Contents Association Conference
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• 2014.06a
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• pp.307-308
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• 2014

• The Journal of the Korea Contents Association
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• v.13 no.4
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• pp.312-319
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• 2013

The purpose of this study was to evaluate the efficacy of gargle and toothpaste containing low concentration with sodium fluoride on the remineralization through the surface microhardness and mineral content in enamel. After 4 weeks-application, the enamel surface was measured using microhardness tester and the calcium (Ca) and phosphorous (P) concentration of mineral content by electron probe microanalyzer (EPMA) analysis. By combining fluoridated gargle with toothpaste, a remineralized enamel resulted in significantly significant differences among the four groups (p<0.05). The Ca change treated with 0.23% fluoridated toothpaste and 0.02% fluoridated gargle with 0.23% toothpaste demonstrated the highest among the other groups. In conclusion. the fluoridated gargle with toothpaste by low concentration showed a significantly greatest synergistic effect on remineralization of the enamel than the other groups.

• Journal of the korean academy of Pediatric Dentistry
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• v.43 no.4
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• pp.443-451
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• 2016

Subjects of this study were : $FluoroDose^{(R)}$ (FD, Centrix Inc., USA), $Enamelast^{TM}$ (EL, Ultradent Product Inc., USA), $Clinpro^{TM}$ white varnish (CW, 3M ESPE, USA), $CavityShield^{TM}$ (CS, 3M ESPE, USA), V $varnish^{TM}$ (VV, Vericom, Korea), MI $varnish^{TM}$ (MI, GC, Japan). The amount of fluoride ion release was measured eight times during 168 hours to see change in accumulation with the course of time using a measuring instrument. And the remineralization rate was measured with Quantitative Light-induced Fluorescence (QLF). V $varnish^{TM}$ group and MI $varnish^{TM}$ group showed high remineralization rates with statistically significance while $CavityShield^{TM}$ group was the lowest rate of remineralization (p < 0.05). After that, several chosen samples were scanned through electron microscope (SEM). Demineralized enamel was observed as the number of enamel crystal was very small; enamel rods and crystals were highly protruding. Remineralized groups with fluoride varnishes show the decreasing tendency of the surface roughness compared to the demineralized enamel.

• Journal of the korean academy of Pediatric Dentistry
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• v.35 no.3
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• pp.509-515
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• 2008

It is widely believed that the increase in the prevalence of dental erosion is due to soft drinks. The purpose of this study was to assess the influence of soft drinks and etchant on the erosion of tooth enamel and the effect of tooth brushing on the abrasion of eroded enamel, and the effect of milk, saliva and Tooth $Mousse^{(R)}$ on the remineralization. The changes in tooth enamel surface were observed by scanning electron microscophy. The results were as follows; 1. The pH of Coca-Cola and Chilsung-Cider were higher than the pH of FineEtch 37 and $Tyrian^{TM}SPE$. 2. The SEM photos revealed that the pattern of the surface eroded by Coca-Cola and Chilsung-Cider were more obvious than that eroded by etchants. 3. The results of remineralization by saliva, milk, Tooth $Mousse^{(R)}$ were not different from each other. 4. The remineralization by saliva decreased tooth brushing abrasion.

• Journal of the korean academy of Pediatric Dentistry
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• v.31 no.4
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• pp.624-629
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• 2004

The purpose of study was to observe the effect of fluoride and calcium on enamel remineralizaton in vitro. Human premolar enamel specimens were prepared by demineralization in $0.1{\sim}l.0%$ citric acid for 60 minutes. They were remineralized for 6 hours in one of the 1311owing solutions : (1) artificial saliva, (2) artificial saliva with 100ppmF, (3) artificial saliva with 1000ppmF, (4) artificial saliva with 1000ppmCa, and (5) artificial saliva with 100ppmF and 1000ppmCa. No significant remineralization was occurred in artificial saliva and artificial saliva with 100ppmF. Significant remineralization was observed in artificial saliva with 1000ppmF at 3 hours, and in artificial saliva with 1000ppmCa and artificial saliva with 100ppmF and 1000ppmCa at 3 and 6 hours(P<0.05). The remineralization effect of artificial saliva with 100ppmF and 1000ppmCa was greater than that of artificial saliva or artificial saliva with 100ppmF. Addition of F to 100ppm or 1000ppm, addition of Ca to 1000ppm, and increasing the concentration of F from 100ppm to 1000ppm did not significantly increase the remineralization.

• Restorative Dentistry and Endodontics
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• v.22 no.1
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• pp.193-208
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• 1997

Much evidence now exists from both in vivo and in vitro studies to support the claim that small caries lesions can 'heal'. But, there are still different views on the mechanism of remineralization. So in order to find the best condition for the remineralization of incipient dental caries in maximum efficient way, the author conducted the experiment which reveals the effect of pH on remineralization. 40 specimens of sound permanent teeth without demineralization or crack, $100{\mu}m$ in thickness, were immersed in lactic acid buffered demineralization solution for 4 days. Dental caries with surface zone and subsurface lesion were artificially produced. All specimens were immersed in lactic acid buffered remineralization solution of pH 4.0, 4.5, 5.0, 5.5 containg fluoride ion for 10 days. The results were obtaind by observing the specimens for every 10 days under polarized microscopy at x25. 1. Remineralization did not occur in entire depth of body of lesion at given degree of saturation and concentration of fluoride ion. 2. The pattern of remineralization has increased according to increase of pH. So it can be concluded that supersaturated solution with fluoride ion can be affected by pH in remineralization of enamel, and pH 5.5 seems to be very effective in remineralization of deep and surface zone of dental caries. However, more complex factors exist in achieving complete remineralization and further continuous researches are needed to clarify the factors.

• The korean journal of orthodontics
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• v.52 no.1
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• pp.75-79
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• 2022

Objective: To investigate remineralizing effect of three fluoride regimens on artificially demineralized enamel around orthodontic bracket by analyzing mineral density (MD) acquired from micro-computed tomography (micro-CT). Methods: Forty-eight bracket bonded bovine incisors were prepared to create demineralized enamel (DE) surface. The samples were divided into four groups according to the fluoride regimen: 1) no fluoridation, 2) 1.23% acidulated phosphate fluoride (APF) gel, 3) fluoridated toothpaste, and 4) 0.05% sodium fluoride mouthwash. Micro-CT was scanned after demineralization (T0), and 2 weeks (T1) and 4 weeks (T2) of fluoridation. Results: APF gel showed highest remineralization of DE during T1-T0 interval among the groups (p < 0.05); followed by toothpaste, mouthwash and no fluoridation. APF gel and toothpaste demonstrated significant increase in MD after 4 weeks of application (p < 0.05). Conclusions: Remineralization effects of three fluoride regimens were depicted through micro-CT analysis, of which APF gel was most effective.

• Restorative Dentistry and Endodontics
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• v.33 no.6
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• pp.507-517
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• 2008

There are considerable in vitro and in vivo evidences for remineralization and demineralization occurring simultaneously in incipient enamel caries. In order to "heal" the incipient dental caries, many experiments have been carried out to determine the optimal conditions for remineralization. It was shown that remineralization is affected by different pH, lactic acid concentrations, chemical composition of the enamel, fluoride concentrations, etc. Eighty specimens from sound permanent teeth without demineralization or cracks, 0.15 mm in thickness, were immersed in lactic acid buffered demineralization solutions for 3 days. Dental caries with a surface zone and subsurface lesion were artificially produced. Groups of 10 specimens were immersed for 10 or 12 days in lactic acid buffered remineralization solutions consisting of pH 4.3 or pH 6.0, and 100, 50, 25, or 10 mM lactic acid. After demineralization and remineralization, images were taken by polarizing microscopy (${\times}100$) and micro-computed tomography. The results were obtained by observing images of the specimens and the density of the caries lesions was determined. 1.As the lactic acid concentration of the remineralization solutions with pH 4.3 was higher, the surface zone of the carious enamel increased and an isotropic zone of the subsurface lesion was found. However, the total decalcification depth increased at the same time. 2.In the remineralization solutions with pH 6.0, only the surface zone increased slightly but there was no significant change in the total decalcification depth and subsurface zone. In the lactic acid buffer solutions with the lower pH and higher lactic acid concentration, there were dynamic changes at the deep area of the dental carious lesion.