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Genetic diversity and population structure among accessions of Perilla frutescens (L.) Britton in East Asia using new developed microsatellite markers

  • Sa, Kyu Jin;Choi, Ik?Young;Park, Kyong?Cheul;Lee, Ju Kyong
    • Genes and Genomics
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    • v.40 no.12
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    • pp.1319-1329
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    • 2018
  • SSRs were successfully isolated from the Perilla crop in our current study, and used to analyze Perilla accessions from East Asia. Analyses of the clear genetic diversity and relationship for Perilla crop still remain insufficient. In this study, 40 new simple sequence repeat (SSR) primer sets were developed from RNA sequences using transcriptome analysis. These new SSR markers were applied to analyze the diversity, relationships, and population structure among 35 accessions of the two cultivated types of Perilla crop and their weedy types. A total of 220 alleles were identified at all loci, with an average of 5.5 alleles per locus and a range between 2 and 10 alleles per locus. The MAF (major allele frequency) per locus varied from 0.229 to 0.943, with an average of 0.466. The average polymorphic information content (PIC) value was 0.603, ranging from 0.102 to 0.837. The genetic diversity (GD) ranged from 0.108 to 0.854, with an average of 0.654. Based on population structure analysis, all accessions were divided into three groups: Group I, Group II and the admixed group. This study demonstrated the utility of new SSR analysis for the study of genetic diversity and population structure among 35 Perilla accessions. The GD of each locus for accessions of cultivated var. frutescens, weedy var. frutescens, cultivated var. crispa, and weedy var. crispa were 0.415, 0.606, 0.308, and 0.480, respectively. Both weedy accessions exhibited higher GD and PIC values than their cultivated types in East Asia. The new SSR primers of Perilla species reported in this study may provide potential genetic markers for population genetics to enhance our understanding of the genetic diversity, genetic relationship and population structure of the cultivated and weedy types of P. frutescens in East Asia. In addition, new Perilla SSR primers developed from RNA-seq can be used in the future for cultivar identification, conservation of Perilla germplasm resources, genome mapping and tagging of important genes/QTLs for Perilla breeding programs.

Criticism on Cho Ji-hoon's Recognition of Han Yong-un (조지훈의 한용운 인식방법 비판)

  • Lee, Sun-yi
    • Cross-Cultural Studies
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    • v.45
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    • pp.85-107
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    • 2016
  • Cho Ji-hoon was a leading figure on the discussion of recognition on Han Yong-un, particularly the post-liberation period. During the Japanese colonial era, he addressed Han Yong-un as the representative of national poet, and evaluated Han's poems as the models of rebellious nationalistic poetry. Such evaluation by Cho set the precedent of basic perspectives and methodologies on how to recognize Han Yong-un in the present day. This paper analyzes three studies on Han Yong-un, conducted by Cho ji-hoon. We also examine how Cho created his logic of recognizing Han as a national poet, and his poems as nationalistic poetry. Accordingly, this paper has separated recognitions on poet studies and work studies, and further explored how each recognition has consistency with Cho ji-hoon's historical and literary perception. As a result, the following has been concluded: the basis of Cho ji-hoon's recognition on the life and works of Han Yong-un was premised on Cho's understanding of the world from a standpoint of history of ideas, the concept of nation was regarded as an absolute value that binds disparate ideas together, and the combination of nationalism and poetry has been expressed through the logic of nationalistic poetry and the notion which equalizes the poet to a classical scholar. It was further concluded that such equalizing logic contains some logical contradictions derived from integration between universal rights and national sovereignty, and nation and Buddhism. Therefore, it can be said that other possible interpretations on the role of a poet were not fully discussed, but remain bounded. Last but not the least, this paper critically tries to perceive Cho's recognition on Han Yong-un, and accentuates the necessity of new interpretations of Han's poems, apart from those based on nationalism.

Considerations and Alternative Approaches to the Estimation of Local Abundance of Legally Protected Species, the Fiddler Crab, Austruca lactea (법정보호종, 흰발농게(Austruca lactea) 서식 개체수 추정에 대한 검토와 대안)

  • Yoo, Jae-Won;Kim, Chang-Soo;Park, Mi-Ra;Jeong, Su-Young;Lee, Chae-Lin;Kim, Sungtae;Ahn, Dong-Sik;Lee, Chang-Gun;Han, Donguk;Back, Yonghae;Park, Young Cheol
    • Journal of Wetlands Research
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    • v.23 no.2
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    • pp.122-132
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    • 2021
  • We reviewed the methods employed in Korean tidal flat surveys to measure the local abundance of the endangered wildlife and marine protected species, the fiddler crab, Austruca lactea. A complete census for infinite population is impossible even in a limited habitat within a tidal flat, and density estimates from samples strongly vary due to diverse biological and ecological factors. The habitat boundaries and areas shift with periodicities or rhythmic activities of organisms as well as measurement errors. Hence the local abundance calculated from density and habitat areas should be regarded as transient. This conjecture was valid based on the spatio-temporal variations of the density averages, standard error ranges, and spatial distribution of the crab, A. lactea observed for 3 years (2015-2017) in Songdo tidal flat in Incheon. We proposed the potential habitat areas using the occurrence probability of 50% from logistic regression model, reflecting the importance of habitat conservation value as an alternative to local abundance. The spatial shape of potential habitat predicted from a generalized model would remain constant over time unless the species' critical environmental conditions change rapidly. The species-specific model is expected to be used for the introduction of desired species in future habitat restoration/creation projects.

Review of Fish Name on the Fishes of the Family Mugilidae in Korea and Resource Utilization (우리나라 숭어과 어류의 어명 및 자원 활용에 대한 고찰)

  • Ko, Eun Young;Park, Jong Oh;Lee, Kyoung Seon
    • Journal of Marine Life Science
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    • v.4 no.2
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    • pp.96-105
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    • 2019
  • The mugilidae fishes are common euryhaline species that live in coastal marine waters to freshwater areas. The taxonomy and nomenclature of the mugilidae fishes still remain unresolved because of their morphological similarities. Among the mugilidae fishes, most commonly consumed in Korea, are grey mullet (Mugil cephalus) and red lip mullet (Chelon haematocheilus). It is generally called 'mullet' without distinguishing between two mullets. Therefore, the aim of this study is to examine the scientific names and common names of mullet species used in Korea from the domestic journals and Korean old documents. The scientific name of grey mullet is M. cephalus, but that of redlip mullet is C. haematocheilus. But the genus of redlip mullet is still mixed with Chelon, Mugil, and Liza. The standard name of two mullet is not distinguished in the Korean dictionary, but they were clearly distinguished in the Japanese, English, and Chinese dictionaries. In the ancient Korean references, the mullet was called 'Chieo' or 'Sueo'. In most of the old literature, the distinction between grey mullet and redlip mullet is not clear. However, in Jasaneobo, it was written separately from grey mullet and redlip mullet, and attaching "ga" was different from now. The Korean standard name of redlip mullet is 'gasungeo', however, the fishermen in Jeollado and Gyoungsangdo call it 'chamsungeo'. Considering the negative perception of 'ga' character, it is proposed to change 'cham(眞)' instead of 'ga(假)' to improve economic value of red lip mullet.

A Study on Injection Nozzle and Internal Flow Velocity for Removing Air Bubbles inside the Sample Tanks during Hydraulic Rupture Test (수압파열시험 시 시료 탱크 내부 기포 제거를 위한 주입 노즐 및 내부 유속 연구)

  • Yeseung, Lee;Hyunseok, Yang;Woo-Chul, Jung;Dong Hoon, Lee;Man-Sik, Kong
    • Journal of the Korean Institute of Gas
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    • v.26 no.6
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    • pp.9-15
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    • 2022
  • In order to verify the durability of the high-pressure hydrogen tank in the operating pressure range, a hydraulic rupture test should be performed. However, if the bubbles generated by the initial injection process of water are attached to the inner wall of the tank and remain, a sudden pressure change of the bubbles during the rupture of the pressurized tank may cause shock and noise. Therefore, in this study, the flow velocity required to remove the bubbles remaining on the inner wall of the tank was predicted through simplified formulas, and the shape of the injection nozzle to maintain the flow velocity was determined based on the shape of the hydrogen tank for the hydrogen bus. In addition, a numerical model was developed to predict the change in flow velocity according to the inlet pressure, and an experiment was performed through a model tank to prove the validity of the prediction result. As a result of the experiment, the flow velocity near the tank wall was similar to the predicted value of the analysis model, and when the inlet pressure was 1.5 to 5.5 bar, the minimum size of the removable bubble was predicted to be about 2.2 to 4.6 mm.

Protein of Major Germplasms for High-Protein Wheat Breeding (밀 주요 교배모본의 단백질 특성)

  • Ha, Yong-Woong;Song, Hyeon-Suk;Lee, Choon-Ki;Cho, Chang-Hwan
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.35 no.5
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    • pp.393-402
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    • 1990
  • Studies to obtain a fundamental data to improve the quantity and quality of wheat protein and to select the excellent breeding materials suitable for various uses of it were performed on the focuses of protein content, amino acid composition, and its related quality. The range of seed protein content of crossing block consisted with 319 varieties or lines was from 9.9% to 17.6% with the average of 13.2%. The minimum protein content was showed by Wonkwang, and the maximum by Chungnamjaerae and Suwon 210. Among the Korean native varieties or breeding lines of crossing block, at least twelve lines or varieties were selected as the high-protein wheats. In the effects of environmental conditions, the variations of protein content by cultivating locations and year were showed out to be up to 3.6%, and 3.3%, respectively. But the varietal characteristics of wheat protein were seemed to remain constantly. In the results of amino acid analysis, those related with bread quality, except proline were improved with the increase of seed protein. But the improvement of nutritional quality did not agree with the protein content increase of seed. The total content of amino acid essential to adults was very low with 266.6-273.3mg per one gram of protein compare to the 350mg (free from the amino acid of tryptophan) recommended by FAO/WHO. Especially the limiting amino acid such as methionine, lysine and threonine were critically deficient. The correlation between protein contents and sedimentation values was obtained r=0.656$\^$**/ of average notwithstanding widly different environmental conditions, and in some condition the value was obained about to r=0.956$\^$**/.

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Efficacy and safety of losartan in childhood immunoglobulin A nephropathy: a prospective multicenter study

  • Hyesun Hyun;Yo Han Ahn;Eujin Park;Hyun Jin Choi;Kyoung Hee Han;Jung Won Lee;Su Young Kim;Eun Mi Yang;Jin Soon Suh;Jae Il Shin;Min Hyun Cho;Ja Wook Koo;Kee Hyuck Kim;Hye Won Park;Il Soo Ha;Hae Il Cheong;Hee Gyung Kang;Seong Heon Kim
    • Childhood Kidney Diseases
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    • v.27 no.2
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    • pp.97-104
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    • 2023
  • Purpose: Angiotensin-converting enzyme inhibitors and angiotensin II receptor blockers (ARBs) are frequently employed to counteract the detrimental effects of proteinuria on glomerular diseases. However, the effects of ARBs remain poorly examined in pediatric patients with immunoglobulin A (IgA) nephropathy. Herein, we evaluated the efficacy and safety of losartan, an ARB, in pediatric IgA nephropathy with proteinuria. Methods: This prospective, single-arm, multicenter study included children with IgA nephropathy exhibiting proteinuria. Changes in proteinuria, blood pressure, and kidney function were prospectively evaluated before and 4 and 24 weeks after losartan administration. The primary endpoint was the difference in proteinuria between baseline and 24 weeks. Results: In total, 29 patients were enrolled and received losartan treatment. The full analysis set included 28 patients who received losartan at least once and had pre- and post-urinary protein to creatinine ratio measurements (n=28). The per-protocol analysis group included 22 patients who completed all scheduled visits without any serious violations during the study period. In both groups, the mean log (urine protein to creatinine ratio) value decreased significantly at 6 months. After 24 weeks, the urinary protein to creatinine ratio decreased by more than 50% in approximately 40% of the patients. The glomerular filtration rate was not significantly altered during the observation period. Conclusions: Losartan decreased proteinuria without decreasing kidney function in patients with IgA nephropathy over 24 weeks. Losartan could be safely employed to reduce proteinuria in this patient population. ClinicalTrials.gov trial registration (NCT0223277)

Community Structure of Pinus densiflora and Quercus mongolica forest in Jochimryeong to Shinbaeryeong of the Baekdudaegan (백두대간 조침령-신배령 구간 소나무림과 신갈나무림의 군락구조)

  • Lee, Ha Young;Kim, Hye Jin;Shin, Hak Sub;Han, Sang Hak;Ko, Seung Yeon;Song, Ju Hyeon;Lee, Jung Hyo;Jang, Kyung Hwan;Yun, Chung Weon
    • Journal of Korean Society of Forest Science
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    • v.103 no.3
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    • pp.339-352
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    • 2014
  • The study was carried out to analyze vegetation structure of Pinus densiflora and Quercus mongolica forests located in Jochimryeong to Shinbaeryeong of the Baekdudaegan mountain range. The survey for 50 plots was conducted from April 2012 to August 2013 in the permanent plots ($100m{\times}100m$) using phytosociological analysis. As a result, the vegetations were classified into five vegetation units. In species composition, they were classified into Q. mongolica community group divided into 2 community such as Fraxinus rhynchophylla community and Carpinus cordata community, F. rhynchophylla community was subdivided Pinus densiflor group (into Euonymus sachalinensis subgroup, Vitis coignetiae subgroup) and Juglans mandshurica group. C. cordata community was subdivided Acer komarovii group and Betula ermanii group. In terms of importance value, P. densiflora and Q. mongolica were more than 20% respectively. P. densiflora was found to have the highest relative coverage. Analysis of interspecific association showed four types which were coincident with differential species and character species on the constancy table. Based on the diameter class distribution, P. densiflora forest presented a normal distribution pattern except for other species which showed a reverse Jshaped distribution pattern, therefore P. densiflora forest would likely be replaced by Q. mongolica forests. While in Q. mongolica forest, diameter class distribution of all species population presented a reverse J-shaped distribution pattern, therefore Q. mongolica forest could likely remain in the future.

Product Recommender Systems using Multi-Model Ensemble Techniques (다중모형조합기법을 이용한 상품추천시스템)

  • Lee, Yeonjeong;Kim, Kyoung-Jae
    • Journal of Intelligence and Information Systems
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    • v.19 no.2
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    • pp.39-54
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    • 2013
  • Recent explosive increase of electronic commerce provides many advantageous purchase opportunities to customers. In this situation, customers who do not have enough knowledge about their purchases, may accept product recommendations. Product recommender systems automatically reflect user's preference and provide recommendation list to the users. Thus, product recommender system in online shopping store has been known as one of the most popular tools for one-to-one marketing. However, recommender systems which do not properly reflect user's preference cause user's disappointment and waste of time. In this study, we propose a novel recommender system which uses data mining and multi-model ensemble techniques to enhance the recommendation performance through reflecting the precise user's preference. The research data is collected from the real-world online shopping store, which deals products from famous art galleries and museums in Korea. The data initially contain 5759 transaction data, but finally remain 3167 transaction data after deletion of null data. In this study, we transform the categorical variables into dummy variables and exclude outlier data. The proposed model consists of two steps. The first step predicts customers who have high likelihood to purchase products in the online shopping store. In this step, we first use logistic regression, decision trees, and artificial neural networks to predict customers who have high likelihood to purchase products in each product group. We perform above data mining techniques using SAS E-Miner software. In this study, we partition datasets into two sets as modeling and validation sets for the logistic regression and decision trees. We also partition datasets into three sets as training, test, and validation sets for the artificial neural network model. The validation dataset is equal for the all experiments. Then we composite the results of each predictor using the multi-model ensemble techniques such as bagging and bumping. Bagging is the abbreviation of "Bootstrap Aggregation" and it composite outputs from several machine learning techniques for raising the performance and stability of prediction or classification. This technique is special form of the averaging method. Bumping is the abbreviation of "Bootstrap Umbrella of Model Parameter," and it only considers the model which has the lowest error value. The results show that bumping outperforms bagging and the other predictors except for "Poster" product group. For the "Poster" product group, artificial neural network model performs better than the other models. In the second step, we use the market basket analysis to extract association rules for co-purchased products. We can extract thirty one association rules according to values of Lift, Support, and Confidence measure. We set the minimum transaction frequency to support associations as 5%, maximum number of items in an association as 4, and minimum confidence for rule generation as 10%. This study also excludes the extracted association rules below 1 of lift value. We finally get fifteen association rules by excluding duplicate rules. Among the fifteen association rules, eleven rules contain association between products in "Office Supplies" product group, one rules include the association between "Office Supplies" and "Fashion" product groups, and other three rules contain association between "Office Supplies" and "Home Decoration" product groups. Finally, the proposed product recommender systems provides list of recommendations to the proper customers. We test the usability of the proposed system by using prototype and real-world transaction and profile data. For this end, we construct the prototype system by using the ASP, Java Script and Microsoft Access. In addition, we survey about user satisfaction for the recommended product list from the proposed system and the randomly selected product lists. The participants for the survey are 173 persons who use MSN Messenger, Daum Caf$\acute{e}$, and P2P services. We evaluate the user satisfaction using five-scale Likert measure. This study also performs "Paired Sample T-test" for the results of the survey. The results show that the proposed model outperforms the random selection model with 1% statistical significance level. It means that the users satisfied the recommended product list significantly. The results also show that the proposed system may be useful in real-world online shopping store.

Seasonal and Yearly Variations of Atmospheric Extinction Coefficient at Campus Station of Chungbuk National University Observatory from 2005 to 2007 (충북대학교 천문대 교내관측소에서 측정된 2005년부터 2007년까지의 대기소광계수의 계절별, 년도별 변화)

  • Kim, Chun-Hwey;Cha, Sang-Mok;Choi, Young-Jae;Song, Mi-Hwa;Park, Jang-Ho;Won, Jang-Hee;Yim, Jin-Sun;Cho, Myung-Shin;Park, Eun-Mi;Jeong, Jang-Hae
    • Journal of Astronomy and Space Sciences
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    • v.25 no.2
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    • pp.101-112
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    • 2008
  • Systematic CCD observations of times of minimum lights for eclipsing binaries has been carried out from 2002 to 2007 at Campus Station of Chungbuk National University Observatory which is located in Cheongju city, Korea. As a by-product of our observations, photometric data for stars in CCD images taken from 2005 to 2007 were used to determine 1st order atmospheric extinction coefficient (hereafter AEC) and seasonal and yearly variations of the AECs were studied. Total nights used for determination of AECs were 57 days in 2005, 51 days in 2006, and 63 days in 2007. As a result the annual mean value of the AECs per air mass is calculated as $0.^m34{\pm}0.^m18$ for 2005, $0.^m38{\pm}0.^m19$ for 2006, and $0.^m45{\pm}0.^m20$ for 2007. These values show that the AECs and their standard deviations are two and four times, respectively, larger than those of normal observatories which are not located near large cities. Annual comparison between concentration of atmospheric fine dust and coefficient of atmospheric extinction show strong correlation between two quantities of which time variations show similar patterns. The AECs for the east sky show larger than those for the west sky. It can be easily understood by the reasonable possibility that air pollutants remain more in the east sky than in the west because the east area of Cheongju city has been more developed than the west one. In conclusion the atmospheric extinction of the night sky of Cheongju city has an annual trend of increase of $0.^m06\;airrnass^{-1}\; year^{-1}$ implying that it may take only about 13 years for Cheongju city to have 2 times brighter night sky than the present one. Our study highlights that variations of AEC can be used as an important indicator of air pollution to monitor night skies.