• Biomolecules & Therapeutics
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• 제31권2호
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• pp.193-199
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• 2023

In this investigation, we made a study of the efficacy of luteolin (a flavonoid found in plants such as vegetables, herbs and fruits) on vascular contractibility and to elucidate the mechanism underlying the relaxation. Isometric contractions of denuded muscles were stored and combined with western blot analysis which was conducted to assess the phosphorylation of myosin phosphatase targeting subunit 1 (MYPT1) and phosphorylation-dependent inhibitory protein for myosin phosphatase (CPI-17) and to examine the effect of luteolin on the RhoA/ROCK/CPI-17 pathway. Luteolin significantly alleviated phorbol ester-, fluoride- and thromboxane mimetic-elicited contractions regardless of endothelial nitric oxide synthesis, implying its direct effect on smooth muscle. It also significantly alleviated the fluoride-elicited elevation in pCPI-17 and pMYPT1 levels and phorbol 12,13-dibutyrate-elicited increase in pERK1/2 level, suggesting depression of ROCK and PKC/MEK activity and ensuing phosphorylation of MYPT1, CPI-17 and ERK1/2. Taken together, these results suggest that luteolin-elicited relaxation includes myosin phosphatase reactivation and calcium desensitization, which seems to be arbitrated by CPI-17 dephosphorylation via ROCK/PKC inhibition.

• Biomolecules & Therapeutics
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• 제30권2호
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• pp.145-150
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• 2022

In this study, we investigated the influence of galangin on vascular contractibility and to determine the mechanism underlying the relaxation. Isometric contractions of denuded aortic muscles were recorded and combined with western blot analysis which was performed to measure the phosphorylation of phosphorylation-dependent inhibitory protein of myosin phosphatase (CPI-17) and myosin phosphatase targeting subunit 1 (MYPT1) and to evaluate the effect of galangin on the RhoA/ROCK/CPI-17 pathway. Galangin significantly inhibited phorbol ester-, fluoride- and thromboxane mimetic-induced vasoconstrictions regardless of endothelial nitric oxide synthesis, suggesting its direct effect on vascular smooth muscle. Galangin significantly inhibited the fluoride-dependent increase in pMYPT1 and pCPI-17 levels and phorbol 12,13-dibutyrate-dependent increase in pERK1/2 level, suggesting repression of ROCK and MEK activity and subsequent phosphorylation of MYPT1, CPI-17 and ERK1/2. Taken together, these results suggest that galangin-induced relaxation involves myosin phosphatase reactivation and calcium desensitization, which appears to be mediated by CPI-17 dephosphorylation via not PKC but ROCK inactivation.

• 한국해양학회지
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• 제18권2호
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• pp.149-160
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• 1983

해수면과 연안표층수온의 기록으로부터 바람에 의한 용승효과를 확인하기 위해서 1973년 부터 1979년까지의 자료들이 이용되었다. 하계에 남동해안에 평행한 바람이 우세하며 울기-감포 근해에서 용승을 일으키는 것으로 나타났는데 강한 남서풍이 3일 이상지속될 때 해수면과 표층수온이 감소하는 용승효과가 현저하게 나타났다. 평균적으로 한 번의 용승은 약10일동안 지속 되었고 그 동안에 해안에 평행안 성분의 평균풍속은 약 4.0m/sec였다.

• 감성과학
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• 제5권1호
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• pp.17-24
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• 2002

본 연구는 객관적인 생리신호로부터 인간의 감성을 추론할 수 있는 감성평가 전문가 시스템을 개발하기 위한 첫 번째 단계로 측정된 생리신호를 이용하여 인간의 긴장도를 판단하는 알고리즘의 개발을 목표로 한다. 감성평가와 관련된 애매함을 수리적으로 취급하기 위해 퍼지이론을 적용하여 임의의 감성영역에 속하는 정도를 소속함수로 정량화함으로써 감성평가를 가능하게 하고자 하였다. 소속함수의 결정은 상상을 통해 유발된 긴장/이완의 생리신호 데이터베이스 결과를 사용하였다. 그리고 두 가지 이상의 생리신호 측정결과와 각 생리신호의 소속함수로부터 하나의 최종결과(긴장도)를 유추하기 위해서 Dempster-Shafer증거합 법칙을 적용하였고, 이를 통해 최종적인 긴장도를 도출할 수 있도록 하였다.

• 경영과학
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• 제31권3호
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• pp.61-76
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• 2014

There are three methods for calculating the optimal level for spare part inventories in a MIME (Multi Indenture and Multi Echelon) system : marginal analysis, Lagrangian relaxation method, and genetic algorithm. However, their solutions are sub-optimal solutions because the MIME system is neither convex nor separable by items. To be more specific, SRUs (Shop Replaceable Units) are required to fix a defected LRU (Line Replaceable Unit) because one LRU consists of several SRUs. Therefore, the level of both SRU and LRU cannot be calculated independently. Based on the limitations of three existing methods, we proposes a improved algorithm applying marginal analysis on determining LRU stock level and genetic algorithm on determining SRU stock level. It can draw optimal combinations on LRUs through separating SRUs. More, genetic algorithm enables to extend the solution search space of a SRU which is restricted in marginal analysis applying greedy algorithm. In the numerical analysis, we compare the performance of three existing methods and the proposed algorithm. The research model guarantees better results than the existing analytical methods. More, the performance variation of the proposed method is relatively low, which means one execution is enough to get the better result.

• 지역사회간호학회지
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• 제13권1호
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• pp.17-29
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• 2002

This study was carried out to investigate the effect of Self-foot reflexology (SFR) on the hypertension of workers. The purpose of the research was to evaluate: levels of knowledge, physical and emotional condition, work stress and fatigue on the hypertension of employees in the workplace. Quasi-experimental study was designed in the setting of a nonequivalent control and experimental Group applied by the pre and post test. The total subjects undertaken in the study were total of 34 employees working in three companies in Seoul. The SFR program was consisted of 6 phases. There were 2 minutes for preparation, 4 minutes for slow down, 26 minutes for base reflex, symptoms of a disease reflex and excretion reflex stimulation. Finally, there was 20 minutes relaxation in a comfortable posture and drinking hot water after blood pressure was checked in the order. This program was running 55 minutes a day everyday three times a week during the entire 8 week course. The finding showed blood pressure was reduced significantly. Moreover, the level of total cholesterol. high and low density lipoprotein cholesterol, depression, work stress, and fatigue were decreased in the study. But, they were not statistically significant except as it related to Group comparisons in time. The level of state anxiety was statistically significant between 2 Groups, but not in the time comparison of both Groups. As a summary of the study results, the SFR program was regarded as contributing to the physical and emotional promotion of employees. It had partially increased body circulations of functional organ related to the SFR sites. And, it improved relaxation of physical and mental condition through energy movement 'Chi'. Therefore, the SFR technique should be considered as an effective skill of a nursing program. Furthermore, it can be newly adopted as a nursing curriculum as a part of alternative treatment. However, it still needed to testify its effects through the review study.

• Biomolecules & Therapeutics
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• 제26권4호
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• pp.374-379
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• 2018

In this study, we investigated the effects of pelargonidin, an anthocyanidin found in many fruits and vegetables, on endothelium-independent vascular contractility to determine the underlying mechanism of relaxation. Isometric contractions of denuded aortic muscles from male rats were recorded, and the data were combined with those obtained in western blot analysis. Pelargonidin significantly inhibited fluoride-, thromboxane A2-, and phorbol ester-induced vascular contractions, regardless of the presence or absence of endothelium, suggesting a direct effect of the compound on vascular smooth muscles via a different pathway. Pelargonidin significantly inhibited the fluoride-dependent increase in the level of myosin phosphatase target subunit 1 (MYPT1) phosphorylation at Thr-855 and the phorbol 12,13-dibutyrate-dependent increase in the level of extracellular signal-regulated kinase (ERK) 1/2 phosphorylation at Thr202/Tyr204, suggesting the inhibition of Rho-kinase and mitogen-activated protein kinase kinase (MEK) activities and subsequent phosphorylation of MYPT1 and ERK1/2. These results suggest that the relaxation effect of pelargonidin on agonist-dependent vascular contractions includes inhibition of Rho-kinase and MEK activities, independent of the endothelial function.

• Natural Product Sciences
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• 제20권3호
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• pp.216-225
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• 2014

The present study was designed to investigate whether ethylacetate (EtOAc) fraction extracted from Rubus coreanum affect the contractility of the isolated thoracic aortic strips and blood pressure of normotensive rats. The EtOAc fraction ($400{\mu}g/mL$) significantly depressed both phenylephrine (PE, $10{\mu}M$)- and high $K^+$ (56 mM)-induced contractile responses of the isolated thoracic aortic strips in a concentration-dependent fashion. In the simultaneous presence of L-NAME (an inhibitor of NO synthase, $300{\mu}M$) and EtOAc ($400{\mu}g/mL$), both PE- and high $K^+$-induced contractile responses were recovered to the corresponding control level in comparison with inhibition of EtOAc-treatment alone. Moreover, in the simultaneous presence of EtOAc after pretreatment with 0.4% CHAPS, both PE- and high $K^+$-induced contractile responses were recovered to the corresponding control level compared to the inhibitory response of EtOAc-treatment alone. Also, in anesthetized rats, EtOAc fraction (0.3~3.0 mg/kg) injected into a femoral vein dose-dependently produced depressor responses. This hypotensive action of EtOAc fraction was greatly inhibited after treatment with phentolamine (1 mg/kg), chlorisondamine (1 mg/kg), L-NAME (3 mg/kg/30 min) or sodium nitroprusside ($30{\mu}g/kg/30 min$). Intravenous infusion of EtOAc fraction (1.0~10.0 mg/kg/30 min) markedly inhibited norepinehrine-induced pressor responses. Taken together, these results demostrate that EtOAc causes vascular relaxation in the isolated rat thoracic aortic strips as well as hypotensive action in anesthetized rats. These vasorelaxation and hypotension of EtOAc seem to be mediated at least by the increased NO production through the activation of NO synthase of vascular endothelium, and the inhibitory adrenergic modulation.

• 대한의용생체공학회:의공학회지
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• 제20권2호
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• pp.191-198
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• 1999

바이오피드백은 혈압 심박율, 말초체온, 호흡, 근전도 등과 같이 자율신경에 의해 지배받는 신체 기능들을 훈련을 통해 스스로 조절할 수 있게 하여 자율신경의 이상으로 인한 여러 가지 질병들을 치료할 수 있게 하는 방법이다. 븐 논문에서는 주정중독증 환자들을 대상으로 바이오피드백 훈련을 실시할 수 있도록 근전도, 호흡, 맥파, 말초체온, 피부전기전도도 등 다섯 가지 신호를 측정할 수 있는 바이오피드백 시스템을 개발하였다. 개발된 시스템을 이용해 주정중독증 환자들을 대상으로 바이오피드백 훈련을 실시하였다 이완 바이오피드백 훈련을 실행하였으며 생체신호변화에 대해 퍼지이론을 적용하여 긴장상태를 판단하였다. 8명의 주정중독증 환자들이 실험에 참가하였으며 측정된 데이터를 분석한 결과는 다음과 같다. 1) 환자 군은 일반인에 비해서 높은 긴장도 값을 보였다. 2) 환자들의 긴장도 값은 훈련 회수가 증가함에 따라 감소하는 추세를 보였다.

• 대한통합의학회지
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• 제10권1호
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• pp.129-136
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• 2022

Purpose : The purpose of this study was to investigate the effect of applying intensity on the muscle activity and muscle hardness of the upper trapezius muscle in adult males when TENS (transcutaneous electrical nerve stimulation) was applied. In addition, this study intends to prepare the scientific basic data of TENS for the purpose of relaxation based on the experimental results. Methods : Eighty-seven healthy adult males participated in the experiment, and they were randomly divided into an experimental group and a control group. All subjects in this study were healthy subjects without musculoskeletal or nervous system damage. All subjects were subjects who voluntarily consented to the purpose and method of the experiment. All subjects were provided with a load by typing for 20 minutes, and muscle activity and muscle hardness of the upper trapezius muscle were measured immediately. Afterwards, TENS was given to each groups for 15 minutes, and the experimental group received stimulation at the motor threshold level, and the control group received a placebo stimulation. After stimulation, muscle activity and muscle hardness of the upper trapezius muscle were measured in the same method. The measured data were compared between groups through an independent t-test and dependent t-test. The statistical significance level was set at .05. Results : The application of TENS statistically significantly decreased the muscle activity and muscle hardness of the trapezius muscle in the experimental group, and the results showed a significant difference from the control group. Conclusion : Application of TENS significantly decreased the muscle activity and muscle stiffness of the upper trapezius muscle. The application of TENS of applying intensity that induces muscle contraction may induce relaxation by reducing the muscle activity and muscle hardness of the trapezius muscle.