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Forage Quality Evaluation of Mutant Lines derived from Gamma-ray Treatments in Rubus fructicosus L. (감마선 조사 유래 블랙베리(Rubus fructicosus L.) 돌연변이 계통의 농업부산물 사료가치 분석)

  • Ryu, Jaihyunk;Kim, Dong sub;Ha, Bo-Keun;Kim, Jin-Baek;Kim, Sang Hoon;Ahn, Joon-Woo;Jeong, Il Yun;Jo, Han-Jik;Kim, Ee-Yup;Kang, Si-Yong
    • Journal of Radiation Industry
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    • v.7 no.1
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    • pp.37-43
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    • 2013
  • We examined the forage quality to provide the basic information for the utilization of blackberry by-products (leaf and stem) among thirty-five mutant lines derived from gamma-ray. The leaves had higher crude protein (CP) contents than stems. In addition, the leaves showed broad ranges of variation in crude fat, crude fiber and crude ash. The overall forage quality of the stems were similar to those of the original variety. The mean of neutral detergent fiber (NDF) content of leaves and stems in mutant lines were 25.88% and 58.98%, respectively. Also, the mean of acid detergent fiber (ADF) content of leaves and stems in mutant lines were 15.43% and 49.27%, respectively. Forage quality grades of all blackberry leaf were special quality class. However, the blackberry stem ranked $1^{st}$ to $5^{th}$ in quality class. The stem diameter was negatively ($P{\leq}0.05$) correlated with the moisture and crude protein. Also, the fruit length was highly negatively ($P{\leq}0.01$) correlated with the RFV (relative feed value) in blackberry leaf. The one hundred fruit weight was highly negatively ($P{\leq}0.01$) correlated with the crude ash. Based on these results, the by-products of blackberry could provide high quality forage for feeding of live stock.

Cloning and Expression of a Alkaline Protease from Bacillus clausii I-52 (Bacillus clausii I-52로부터 alkaline protease 유전자의 클로닝 및 발현)

  • Joo, Han-Seung;Choi, Jang Won
    • Journal of agriculture & life science
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    • v.45 no.6
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    • pp.201-212
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    • 2011
  • The alkaline protease gene was cloned from a halo-tolerant alkalophilic Bacillus clausii I-52 isolated from the heavily polluted tidal mud flat of West Sea in Inchon Korea, which produced a strong extracellular alkaline protease (BCAP). Based on the full genome sequence of Bacillus subtilis, PCR primers were designed to allow for the amplification and cloning of the intact pro-BCAP gene including promoter region. The full-length gene consists of 1,143 bp and encodes 381 amino acids, which includes 29 residues of a putative signal peptide and an additional 77-amino-acid propeptide at its N-terminus. The mature BCAP deduced from the nucleotide sequence consists of 275 amino acids with a N-terminal amino acid of Ala, and a relative molecular weight and pI value was 27698.7 Da and 6.3, respectively. The amino acid sequence shares the highest similarity (99%) to the nattokinase precursor from B. subtilis and subtilisin E precursor from B. subtilis BSn5. The substrate specificity indicated that the recombinant BCAP could hydrolyze efficiently the synthetic substrate, N-Suc-Ala-Ala-Pro-Phe-pNA,and did not hydrolyze the substrates with basic amino acids at the P1 site. The recombinant BCAP was strongly inhibited by typical serine protease inhibitor, PMSF, indicating that BCAP is a member of the serine proteases.

Characteristics of Soil Water Runoff and Canopy Cover Subfactor in Sloped Land with Different Soil Texture (경사지 밭토양에서 강우량과 토성에 따른 물 유출 양상 및 수관피복인자 구명)

  • Lee, Hyun-Haeng;Ha, Sang-Keon;Hur, Seung-Oh;Jung, Kang-Ho;Park, Chan-Won;Kim, Kye-Hoon
    • Korean Journal of Soil Science and Fertilizer
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    • v.40 no.2
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    • pp.131-135
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    • 2007
  • This study was performed as an effort to reduce soil loss by investigating the phase of water flow according to soil texture and rainfall pattern and by determining the canopy cover subfactor in the RUSLE (revised universal soil loss equation). Red pepper was planted at the 15% sloped lysimeter of $2m{\times}5m{\times}0.5m$ ($width{\times}length{\times}depth$) with three different textured soils (loam, clay loam and sandy loam) and the relationship between amount and intensity of rainfall; soil loss and the amount of runoff; and amount of rainfall and runoff at different soil texture were measured at the experiment station of the National Institute of Agricultural Science and Technology (NIAST) during May to October of 2005. The amount of runoff increased with increasing amount of rainfall, showing difference in the relative increase rate of runoff at different soil texture. The increase rate of runoff with unit increase of rainfall for the lysimeter with red pepper was 0.44, 0.41 and 0.13 for loam, clayey loam and sandy loam, respectively. The minimum amount of rainfall for runoff was 23.53 mm for sandy loam, 10.35 mm for loam and 5.46 mm for clayey loam, respectively. The canopy cover subfactors of red pepper were 0.425, 0.459, and 0.478 for sandy loam, loam and clayey loam, respectively.

The Interdigitated-Type Capacitive Humidity Sensor Using the Thermoset Polyimide (열경화성 폴리이미드를 이용한 빗살전극형 정전용량형 습도센서)

  • Hong, Soung-Wook;Kim, Young-Min;Yoon, Young-Chul
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.6
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    • pp.604-609
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    • 2019
  • In this study, we fabricated a capacitive humidity sensor with interdigitated (IDT) electrodes using a thermosetting polyimide as a humidifying material. First, the number of electrodes, thickness, and spacing of the polyimide film were optimized, and a mask was designed and fabricated. The sensor was fabricated on a silicon substrate using semiconductor processing equipment. The area of the sensor was $1.56{\times}1.66mm^2$, and the width of the electrode and the gap between the electrodes were each $3{\mu}m$. The number of electrodes was 166, and the length of an electrode was 1.294 mm for the sensitivity of the sensor. The sensor was then packaged on a PCB for measurement. The sensor was inserted into a chamber environment with a temperature of $25^{\circ}C$ and connected to an LCR meter to measure the change in capacitance at relative humidity (RH) of 20% to 90%, 1 V, and 20 kHz. The results showed a sensitivity of 26fF/%RH, linearity of < ${\pm}2%RH$, and hysteresis of < ${\pm}2.5%RH$.

Software Package for Pipe Hydraulics Calculation for Single and Two Phase Flow (배관 유동의 주요 변수계산을 위한 소프트웨어 시스템의 개발)

  • Chang, Jaehun;Lee, Gunhee;Jung, Minyoung;Baek, Heumkyung;Lee, Changha;Oh, Min
    • Korean Chemical Engineering Research
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    • v.57 no.5
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    • pp.628-636
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    • 2019
  • In various industrial processes, piping serves as a link between unit processes and is an essential installation for internal flow. Therefore, the optimum design of the piping system is very important in terms of safety and cost, which requires the estimation of the pressure drop, flow rate, pipe size, etc. in the piping system. In this study, we developed a software that determines pressure drop, flow rate, and pipe size when any two of these design variables are known. We categorized the flows into single phase, homogeneous two phase, and separated two phase flows, and applied suitable calculation models accordingly. We also constructed a system library for the calculation of the pipe material, relative roughness, fluid property, and friction coefficients to minimize user input. We further created a costing library according to the piping material for the calculation of the investment cost of the pipe per unit length. We implemented all these functions in an integrated environment using a graphical user interface for user convenience, and C # programming language. Finally, we verified the accuracy of the software using literature data and examples from an industrial process with obtained deviations of 1% and 8.8% for the single phase and two-phase models.

Growth characteristics and Productivity of Italian Ryegrass (Lolium multiflorum Lam.) New Variety, 'Green Call' (이탈리안 라이그라스 신품종 '그린콜'의 생육특성과 수량성)

  • Ji, Hee Chung;Whang, Tae Young;Lee, Ki-Won;Kim, Won Ho;Woo, Jae Hoon;Hong, Ki Hung;Choe, Kuh Wann
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.38 no.4
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    • pp.247-252
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    • 2018
  • This experiment was carried out to breed a very early maturing variety of Italian ryegrass (Lolium multiflorum Lam.) in Grassland and Forage Crops Division, National Institute of Animal Science, RDA, Cheonan from 2015 to 2017. New variety of Ryegrass, 'Green Call' is a diploid variety with green in leaf color and has semi-erect growth habit in late autumn and erect growth habit in early spring, 'Green Call' was on April 25 in heading date as a early-maturing variety. Also 'Green Call' was narrower by 2 mm in flag leaf width, longer by 1.3 cm in flag leaf length and shorter by 5 cm in plant height than those of control variety, 'Florida 80', respectively. 'Green Call' was also thicker by 0.14 mm in stem thickness and strong in winter hardness. Dry matter (DM) yield (11,592 kg/ha) of 'Green Call' was similar to that of 'Florida 80'. Total digestible nutrient (TDN), crude protein (CP) and relative feed value (RFV) of 'Green Call' were 60.8, 10.3 and 96.7 % which are 2.1, 1.1, 6.9 % higher than those of 'Florida 80', respectively. Acid detergent fiber (ADF) and neutral detergent fiber (NDF) of 'Green Call' were 35.5 and 58.9 % which are 2.7 and 2.3 % lower than those of 'Florida 80', respectively.

Comparison of the Accuracy of Stereo Camera Calibration According to the Types of Checkerboards (체커보드의 유형에 따른 스테레오 카메라 캘리브레이션의 정확도 비교)

  • Kim, Eui Myoung;Kwon, Sang Il
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.38 no.6
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    • pp.511-519
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    • 2020
  • For camera calibration, a checkerboard is generally used to determine the principal point, focal length, and lens distortions. The checkerboard has a planar and three-dimensional shape, and camera calibration parameters are affected by the size of the checkerboard, the placement of the target, and the number of target points. In this study, the accuracies of the types of checkerboards were compared using checkpoints for stereo camera calibration, and the purpose of this study was to propose the best performance checkerboard. The checkerboard with large flat shape showed comparatively high accuracy through comparison with the check points. However, due to the size of the checkerboard, it was inconvenient to move and rotate, and there was a disadvantage in that it was difficult to shoot so that the target points could all appear in the stereo camera. The checkerboard, which was manufactured in a small size in a flat shape, was easy to move and rotate but had the lowest three-dimensional accuracy. The checkerboard with targets with height values had the hassle of having to determine the three-dimensional coordinates of the target points by using observation equipment for camera calibration, but it was small in size, convenient to move and rotate, and showed the highest three-dimensional accuracy.

Effects of Breed, Laying Age, and Egg Storage Period on the Vitality of Hatched Chicks in Korean Native Chickens (한국토종닭의 품종, 산란 연령 및 종란의 보관 기간이 병아리의 강건성에 미치는 영향)

  • Choi, Eun Sik;Sohn, Sea Hwan
    • Korean Journal of Poultry Science
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    • v.48 no.1
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    • pp.1-12
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    • 2021
  • We investigated the effects of breed, laying age, and egg storage period on the vitality of chicks using the Hwanggalsaek Jaeraejong, Korean Rhode Island Red, and Korean White Leghorn chicken breeds. Their eggs were collected during the early laying period (27~29 weeks) and late laying period (50~52 weeks) and were stored for 3 days, 7 days and 14 days. After the eggs hatched, the hatching time, production performance, and organ weight of chicks were investigated. IL-6 gene expression level and relative length of telomeres were analyzed to determine the physiological activity of the chicks. HSP gene expression level and heterophil to lymphocytes ratio were also analyzed to examine the degree of stress response in the chicks. The results showed that breed and laying age influenced the vitality of chicks, but the egg storage period did not. Korean Leghorn chicks were considered the weakest breed in terms of vitality owing to their low survival rate, small heart size, low physiological activity, and high stress response level. Although the survival rate of chicks produced in the early laying period was low, their high physiological activity and low stress response indicated that they had a high vitality than the chicks produced in the late laying period. In conclusion, to obtain chicks with high vitality, it would be desirable to select a high vitality breed and avoid the use of chicks produced in the late laying period.

Comparison of Growth Characteristics and Forage Productivity of Italian Ryegrass in Yeongseo and Yeongdong of Gangwon Province (강원 영서 및 영동지역에서 이탈리안 라이그라스 생육특성과 생산성 비교)

  • Lee, Hong-Ju;Byeon, Ji-Eun;Ryoo, Jong-Won;Hwang, Sun-Goo
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.40 no.4
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    • pp.251-258
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    • 2020
  • To investigate the suitable growing area for Italian ryegrass (cv. Kowinearly and cv. Green-Call), we survey the different effects of climatic conditions on plant growth in the east (hereafter termed Yeongdong) and west (hereafter termed Yeongseo) of the passes in Gangwon Province. The Italian ryegrass was grown in Gangneung of Yeongdong and Wonju and Chuncheon of Yeongseo. The plants showed growth differences depending on the cultivated regions between before and after wintering. The Italian ryegrass for pre-wintering showed the relatively long length of plant height in Wonju. While, we observed the relatively tall plants for growing- and harvesting-period after wintering in Gangneung. The increased plant height in Gangneung was closely related to all climatic conditions for the growing period and mean- and lowest-temperature for the harvesting period, respectively. The amount of dry matter was 7,490 kg/ha for Kowinearly and 6,490 kg/ha for Green-Call in Gangneung, which has a higher yield than Chuncheon and Wonju. The relative yield index of dry matter was 77% (Kowinearly) and 78% (Green-Call) in Chuncheon and 84% (Kowinearly) and 71% (Green-Call) in Wonju compared to Gangneung as the standard region. Thus, we suggest that Chuncheon and Wonju are the possible areas for cultivation of Italian ryegrass, considering that Gangneung was the optimum growing area in Gangwon Province.

Comparison of Stomach Contents of Yellow Goosefish Lophius litulon, in the South Sea and Yellow Sea, Korea (한국 남해와 서해에서 출현하는 황아귀(Lophius litulon)의 위내용물 조성 비교)

  • Kim, Do-Gyun;Jeong, Jae Mook;Lee, Seung-Jong;Kang, Sukyung;Seong, Gi Chang;Kang, Da Yeon;Jin, Suyeon;Baeck, Gun Wook
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.55 no.5
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    • pp.714-720
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    • 2022
  • A dietary comparison of yellow goosefish Lophius litulon in the South Sea and Yellow Sea was performed by analyzing stomach contents. Using a bottom trawl net from fisheries resources survey vessels of the National Institute of Fisheries Science, 326 individuals were collected from the South Sea and 166 individuals were collected from the Yellow Sea. The total length range of L. litulon was 5.3-68.4 cm for individuals collected from the South Sea and 7.5-55.1 cm for individuals collected from the Yellow Sea. Based on the index of relative importance and the variation in stomach contents by fish size, L. litulon was found to be a piscevore and a spatiotemporally opportunistic feeder fish that fed mainly on pisces. There were differences in the composition of the stomach contents by season, location, and the interaction between the season and the location. Thus, the diet of L. litulon is affected by the prey abundance and prey spectrum of the habitat.