• Journal of Microbiology and Biotechnology
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• v.26 no.12
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• pp.2106-2115
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• 2016

To identify the global effects of (p)ppGpp in the gram-positive bacterium Deinococcus radiodurans, which exhibits remarkable resistance to radiation and other stresses, RelA/SpoT homolog (RSHs) mutants were constructed by direct deletion mutagenesis. The results showed that RelA has both synthesis and hydrolysis domains of (p)ppGpp, whereas RelQ only synthesizes (p)ppGpp in D. radiodurans. The growth assay for mutants and complementation analysis revealed that deletion of relA and relQ sensitized the cells to $H_2O_2$, heat shock, and amino acid limitation. Comparative proteomic analysis revealed that the bifunctional RelA is involved in DNA repair, molecular chaperone functions, transcription, the tricarboxylic acid cycle, and metabolism, suggesting that relA maintains the cellular (p)ppGpp levels and plays a crucial role in oxidative resistance in D. radiodurans. The D. radiodurans relA and relQ genes are responsible for (p)ppGpp synthesis/hydrolysis and (p)ppGpp hydrolysis, respectively. (p)ppGpp integrates a general stress response with a targeted re-programming of gene regulation to allow bacteria to respond appropriately towards heat shock, oxidative stress, and starvation. This is the first identification of RelA and RelQ involvement in response to oxidative, heat shock, and starvation stresses in D. radiodurans, which further elucidates the remarkable resistance of this bacterium to stresses.

• Journal of Power Electronics
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• v.3 no.2
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• pp.115-123
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• 2003

This paper presents analysis, design and simulation for the indirect field orientation control (IFOC) of induction machine drive system. The dynamic performance of the IFOC under nominal and detuned parameters of the induction machine is established. A conventional proportional plus integral-derivative (PI-D) two-degree-of-freedom controller (2DOFC) is designed and analysed for an ideal IFOC induction machine drive at nominal parameters with the desired dynamic response. Varying the induction machine parameters causes a degredation in the dynamic response for disturbance rejection and tracking performance with PI-D 2DOF speed controller. Therefore, conventional controllers can nut meet a wide range of speed tracking performance under parameter variations. To achieve high- dynamic performance, a proposed robust fuzzy logic controllers (RFLC) for d-axis rotor flux, d-q axis stator currents and rotor speed have been designed and analysed. These controllers provide robust tracking and disturbance rejection performance when detuning occurres and improve the dynamic behavior. The proposed REL controllers provide a fast and accurate dynamic response in tracking and disturbance rejection characteristics under parameter variations. Computer simulation results demonstrate the effectiveness of the proposed REL controllers and a robust performance is obtained fur IFOC induction machine drive system.