• Journal of Plant Biotechnology
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• 제36권1호
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• pp.53-58
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• 2009

Calcium signals can be transduced by binding calmodulin (CaM), a $Ca^{2+}$ sensor in eukaryotes, is known to be involved in the regulation of diverse cellular functions. We isolated a CaM-binding protein 63 kD (AtCBP63) from the pathogen-treated Arabidopsis cDNA expression library. Recently, AtCBP63 was identified as a CaM bining protein. The CaM binding domain of AtCBP63 was reported to be located in its N-terminal region, In this study, however, we showed that ACaM2 could specifically bind to second CaM-binding domain (CaMBD) of AtCBP63 at the C-terminal region. The specific binding of CaM to CaM binding domain was confirmed by a gel mobility shift assay, a split ubiquitin assay, site-directed mutagenesis, and a competition assay using a $Ca^{2+}$/CaM-dependent enzyme. The gene expression of AtCBP63 was induced by pathogens and pathogens related second messengers. This result suggests that a CaM binding protein, AtCBP63, may play role in pathogen defense signaling pathway.

• The Plant Pathology Journal
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• 제22권2호
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• pp.139-146
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• 2006

The 3' region of Potato virus X (PVX) has the 74 nt 3'-nontranslated region (NTR) that is conserved among all potexviruses and contains several cis-acting elements for minus-strand and plus-strand RNA accumulation. Three stem-loop structures (SL1-SL3), especially formation of SL3 and U-rich sequence of SL2, and near upstream elements in the 3' NTR were previously demonstrated as important cis-acting elements. To Investigate the binding of these cis-acting elements within 3' end with host protein, we used the electrophoretic mobility shift assays (EMSA) and UV-cross linking analysis. The EMSA with cellular extracts from tobacco and RNA transcripts corresponding to the 150 nt of the 3' end of PVX RNA showed that the 3' end of PVX formed complexes with cellular proteins. The specificity of protein binding was confirmed through competition assay by using with 50-fold excess of specific and non-specific probes. We also conducted EMSA with RNAs containing various mutants on those cis-acting elements (${\Delta}10$10, SL3B, SL2A and ${\Delta}21$; J Mol Biol 326, 701-720) required for efficient PVX RNA accumulation. These analyses supported that these cis-acting elements are required for interaction with host protein(s). UV-cross linking analysis revealed that at least three major host proteins of about 28, 32, and 42 kDa in mass bound to these cis-elements. These results indicate that cis-acting elements from 3' end which are important for minus and plus-strand RNA accumulation are also required for host protein binding.

• 한국정보과학회논문지:소프트웨어및응용
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• 제31권2호
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• pp.189-202
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• 2004

본 논문에서는 영상 입력 장치로 입력되는 영상 내의 수많은 정보 중에서 지각적으로 중요하다고 여겨지는 현저한(salient) 영역만을 탐지해내는 새로운 방법을 제안한다. 제안하는 방법은 인간이 가지고 있는 시각적 주의 기능에 기본 바탕을 두고 있으며, 영상을 구성하고 있는 정보의 특징에 기반을 두고 있다. 가장 먼저 인간의 시각적 주의 기능에 영향을 미친다고 알려져 있는 몇 가지 특징들이 입력되는 영상의 모든 영역에 걸쳐 추출되어 각각의 특징에 해당되는 특징지도들로 형성된다. 이렇게 형성된 각각의 특징지도들을 구성하고 있는 특징 값들은 이들 각각의 국부적인 경쟁력 특성에 의하여 영상의 각 영역에서의 중요도를 나타내는 값으로 변환되어 중요도지도를 형성하게 된다. 이러한 중요도지도들은 모두 통합되어 하나의 현저함지도를 생성하게 된다. 현저함지도는 영상 내 각 장소의 현저함 정도를 미리 계산된 특징들의 공간적 중요도 측정치에 따른 스칼라 값으로 표시함으로써 영상 내에서 가장 현저한 영역을 찾을 수 있도록 가이드 한다. 제안하는 방법에 의해 시스템을 구성하여 실험한 결과, 인간이 중요하다고 여겨지는 주요 영역을 만족스럽게 탐지해 냄을 알 수 있었다.

• 대한토목학회논문집
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• 제39권5호
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• pp.623-630
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• 2019

아시아 건설시장의 전략적 중요성이 높아지면서 기업 간 경쟁이 더욱 치열해지고 있다. 기존의 아시아 건설시장에 대한 연구는 주요 국가로의 진출전략 도출, 분야별 리스크 평가에 대한 정성적 접근이 주류를 이루었던 반면, 다수 기업의 역동적인 경쟁구도를 정량적으로 설명한 연구는 드물었다. 이에 본 연구는 국내 건설기업이 2009년부터 2017년까지 계약을 체결한 3,996건의 프로젝트 정보를 바탕으로 아시아 지역의 계약실적 구조를 분석하였다. 정태적 분석에는 시장 집중도, 동태적 분석에는 시장 이동성 및 불안정성 개념을 각각 활용하였으며, 이에 대한 수학적 모델을 소개하였다. 분석 결과, 정태적 측면에서는 아시아 건설시장에서 국내 상위 기업군의 시장 점유율 집중 현상이 나타나고 있으며, 동태적 측면에서는 산업설비 공종을 제외한 대부분의 경우 시장 지위가 약하고 안정성이 떨어지는 것으로 밝혀졌다. 본 연구에서 제시한 분석 방법론과 결과는 산업 차원의 실적에 대한 체계적 진단을 가능케 하며, 향후 경쟁전략 수립을 위한 기초자료를 제공하였다는 점에서 의의가 있다.

• 한국의류산업학회지
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• 제6권1호
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• pp.78-84
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• 2004

To regain competitive advantages in the global textile industry the Korean textile firms need to strategically collaborate each other on developing partnership relations. This study, as a first step for developing a futuristic partnership between textile firms, examined the characteristics of current partnership relations among textile firms in Daegu and Gyeongbuk regions. Data were obtained from 163 textile firms in the region by a questionnaire survey. More than a half of the textile firms had a partnership experience in product manufacturing and wanted to have one in product development. Partnerships with firms in the same sub-industry showed the highest frequency even though various pairs were observed. Length of partnership was related with the history and size of the firm but not with partnership performance. Partnership itself affected neither the firm's perception of its competitive power nor the perceived intensity of industry competition.

• 한국항해항만학회:학술대회논문집
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• 한국항해항만학회 2016년도 춘계학술대회
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• pp.141-142
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• 2016

Port in Northern Vietnam has been playing an important role in the logistics system the country. In recent years, the cargo throughput of the region, especially, containerized cargo has increased gradually. There are totally 11 container terminals in Northern Vietnam, concentrating in Haiphong city and Quang Ninh province. Despite of increasing demand, the competition among the terminals is becoming more and more critical. The paper, firstly, summarizes factors impacting the market share of the terminals before reveal the correlation between given factors and annual container throughput. Factor analysis will be then applied to evaluate the level of terminals' logistics infrastructure. For the conclusion, strategic thinking of terminals' logistics competitiveness will be contributed to related organizations.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2003년도 가을 학술발표회 논문집
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• pp.207-210
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• 2003

As reviewing of current trend on PC connection details, owing to effective stress transfer in the connection, it grow to increase that use of mechanical splices, reinforcements or welded splices, and prestressing. However such devices as reinforcement, mechanical splices entail not only more cost resulted from materials but also extra construction process so as to cause PC used method to lower competition against conventional method. Therefore more enhanced connection details which help working process simplified and construction cost reduced. In this research, as replace 9.3mm 7strand for reinforcement, it is attempt to devise connection detail which makes workability improve and confirm effective stress transfer in the region of connection. The experimental research is proceeded by partial tension test of specimen. The splice lengths of 7strand is decided to be variations. The flexural capability is verified to depend on spice length. An an appropriate splice length could be also determined as a precedent research on improving PC connection detail.

• 대한화학회지
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• 제14권2호
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• pp.161-168
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• 1970

In order to obtain the more effective evidence, supporting the hypothesis which have been previously described by former report that pepsin (EC 3.4. 4.1) forms a hydrophobic bond with the nonpolar side chain of its substrate, the inhibitory effect of carboxylic acids(from formic acid to iso-butyric acid) on the activity of pepsin to the synthetic dipeptide, N-Carbobenzoxy-L-glutamyl-L-tyrosine, was discussed. The kinetic study showed that the inhibition by carboxylic acids was competitive. The Kidecreased with increasing size of the inhibitor molecule. The $-{\Delta}F^{\circ}$increased linearly with increasing number of carbon atoms in the hydrocarbon chain of the inhibitor. It was confirmed that the hydrophobic bond between more than one side chain of amino acid residues(phenylalanine) in the binding region of the active center of pepsin and the side chain of amino acid residues in the substrate was formed as the first step of its enzymic mechanism. The inhibitory effect of carboxylic acids was due to the competition of the hydrocarbon group of the carboxylic acids with the side chain of the substrate for the hydrophobic binding site(the side chain of phenylalanine) of the pepsin.

• 대한안전경영과학회지
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• 제8권1호
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• pp.181-194
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• 2006

Economic and political barriers in Northeast Asia including Korea have been eliminated gradually. As results of economic relations are more active in this region. The administration of physical distribution tightened circulation- cost down. This is one of the most important facts to develop world economy. Distribution in China is expected to grow quantitatively and continuously. China is just out of planned economic system. Therefore, it is expected that foreign affiliated firms will take a fierce competition for the next $1\sim2$ years. This study investigates Korea-China trade and distribution index with analysis and expectation of China market. The study analyzes the business conditions, faced problems and differentiated strategies of foreign affiliated firms. Be based on those, it is researched how domestic companies will make strategies to start China successfully.

• 한국항해항만학회:학술대회논문집
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• 한국항해항만학회 2019년도 춘계학술대회
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• pp.240-241
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• 2019

전 세계 물동량 둔화 및 항만 간 경쟁이 심화되는 가운데, 항만의 환경규제 강화 및 자동화 및 스마트화가 요구되면서 항만산업의 비용증가 요인들이 지속적으로 증가하고 있다. 따라서 본 연구에서는 항만산업의 주요 주체인 우리나라 컨테이너터미널 운영사의 비용자료를 직접 수집하였으며, 이를 통해 컨테이너터미널 운영사의 비용 구조적 특성을 분석하였다. 터미널 운영 규모별, 지역별, 부두 특성별 비용구조의 차이점을 살펴보고 그에 따른 시사점을 제시하였다.