• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 춘계학술대회
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• pp.80-80
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• 2019

This study describes an efficient and widely applicable droplet-vitrification following cryopreservation for shoot tips of (Fragaria x ananassa DUCH. cvs. 'Derunoka' and 'Jumbo pure berry'. The shoot tips of strawberry were precultured in Murashige and Skoog (MS) liquid medium supplemented with sucrose (0.3-0.7M). Precultured explants were treated with loading solution (LS, C4) containing glycerol 17.5% and sucrose 17.5% for 40 min and exposed to dehydration solution (B1) containing 50% of glycerol and 50% of sucrose for 60 min at $25^{\circ}C$, and then transferred onto droplets containing $2.5{\mu}l$ PVS3 on sterilized aluminum foils ($4cm{\times}0.5cm$) prior to direct immersion in liquid nitrogen (LN) for 1 h. The highest regeneration rate (%) was obtained when shoot tips were precultured with treatment-2 (exposing of shoot tips to MS + 0.3M Sucrose for 30 h and then treated with MS+0.5 M sucrose for 16 h) at $25^{\circ}C$ in both the cultivars. The viability of cooled samples, followed by culturing on MS medium for 4 weeks was 77.8% and 60.0% for 'Derunoka' and 'Jumbo pure berry', respectively. This result shows droplet-vitrification would be a promising method for cryobanking strawberry germplasm.

• 한국도서관정보학회지
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• 제50권1호
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• pp.157-176
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• 2019

최근 초고령화 사회, 저출산, 학령인구 감소, 정부의 학교 통폐합 권고 기준 확대 등의 이유로, 지속적으로 폐교가 늘어나고 있는 가운데, 폐교의 유휴시설과 부지를 활용하여 새로운 시설이나 공간으로 재탄생되는 사례가 나타나고 있다. 폐교 활용은 크게 도시재생과 같은 맥락에서 이해할 수 있으며, 이에 본 연구는 도시재생사업에서 쇠퇴지역을 평가하는 기준을 활용하여 폐교 발생 지역의 특징을 확인하고, 폐교 활용 유형을 종합적인 관점에서 분석하고자 하였다. 연구 결과를 통해 도출된 폐교 활용의 유형과 형태, 시사점을 기반으로 폐교 발생 지역의 특성을 확인하고, 이를 바탕으로 도서관의 폐교 활용을 제고하고자 하였으며, 향후 폐교 발생 지역을 유형화하여 폐교 발생 지역의 특징을 고려한 지역별 도서관의 폐교 활용 체계 및 모델이 필요함을 제언하였다.

• 한국산림과학회지
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• 제86권2호
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• pp.128-134
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• 1997

Populus davidiana는 종자의 활력이 짧고(약 3주), 삼목시 발근율이 저조하여 전통적인 유성 무성번식법의 어려움이 있어서 효과적인 기내증식법을 연구하였다. 10가지의 기본 배지를 이용하여, 1-2개의 액아를 포함한 잎이 붙어 있는 줄기절편체를 0.2mg/l의 BAP가 첨가된 배지에서 배양한 결과 MS 배지와 LP배지에서 가장 많은 신초를 생산하였다. 두 배지에서 5주간 배양한 결과 절편체당 9개 이상씩 신초를 생산하였다. 신초생산과 신초의 생장에 있어 클론간의 상당한 차이를 보였다. 몇 클론은 생장조절물질을 첨가하지 않아도 신초를 생산하였다. 기내배양된 신초 중 60% 이상이 0.2mg/l의 IBA가 첨가된 1/2 GD배지에서 발근이 가능하였다.

• Journal of Periodontal and Implant Science
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• 제37권3호
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• pp.479-488
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• 2007

In spite of the attention given to the study of mesenchymal stem cells derived periodontal ligament (PDL), there is a lack of information about canine PDL cells. In this study, we characterized canine PDL cells to clarify their stem cell properties, including self renewal, proliferate rate, stem cell markers and multipotency. PDL cells were obtained from extracted premolars of canines, following a colony forming assay and proliferation rate of sub-confluent cultures of cells for self-renewal, immunostaining for STRO-1 and CD146/MUC18 and a differentiation assay for multipotency. Canine PDL cells formed single-cells colonies and 25% of the PDL cells displayed positive staining for BrdU. The cells expressed the mesenchymal stem-cell markers, STRO-1 and CD146/MUC18. Under defined culture conditions, the cells differentiated into osteoblasts and adipocytes, but the cells didn't differentiated into chondrocytes. The findings of this study indicated that the canine PDL cells possess crucial stem cells properties, such as self-renewal and multipotency, and express the mesenchymal stem cell markers on their surface. The isolation and characterization of canine PDL cells makes it feasible to pursue preclinical models of periodontal regeneration in canine.

• 한국자동차공학회논문집
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• 제18권5호
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• pp.124-129
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• 2010

The direct injection (DI) diesel engine has become a prime candidate for future transportation needs because of its high thermal efficiency. However, nitrogen oxides (NOx) increase in the local high temperature regions and particulate matter (PM) increases in the diffusion flame region within diesel combustion. Therefore, the demand for developing hybrid system consist of exhaust gas recirculation (EGR) and aftertreatment system as well as diesel particulate filter (DPF) or lean NOx trap (LNT) should be applied. The variation of EGR rate due to the malfunction of EGR valve can affect not only the combustion stability of engine but also the performance of aftertreatment system. In this research, 2.0 liter 4-cylinder turbocharged diesel engine was used to investigate the combustion and emission characteristics for various operating conditions with EGR. While the fuel consumption was increased with increase of EGR rate, NOx emission was improved by maximum 90% at low speed, low load operating condition. To achieve combustion stability and reliability of aftertrearment system with minimum penalty in fuel consumption and emissions, the fault diagnosis of EGR malfunction must be employed.

• Journal of Plant Biotechnology
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• 제34권3호
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• pp.181-187
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• 2007

Orostachys japonicus A. Berger is a Perennial herbaceous plant which has been traditionally used as an anti-inflammatory agent to treat hepatitis and as an anticancer agent. The objective of this study was 1) to establish and proliferate in vitro plant of O. japonicus 2) to induce indirect somatic embryogenesis from O. japonicus. General calli and embryogenic calli in all ranges of 2,4-D and BA combination, were induced and were best at 22% (embryogenic cell) in 5.0 mg/L 2,4-D and 0.5 mg/L BA combination. Embryogenic cell line was maintained by subculture at 2 week intervals and transferred to solid and liquid medium for embryo formation. In solid medium culture, globular and heart shaped embryos were observed in MS medium containing 5.0 mg/L 2,4-D and 0.5 mg/L BA combination. The number of embryos was 6.5 per 0.5 g cell, and then the immature embryos transferred to MS basal medium for embryo development. In a suspension culture of embryogenic cells, globular and heart shaped embryos were emerged in MS medium supplemented with 3.0 mg/L 2,4-D and 0.3 mg/L BA combination after 10 days of incubation. The embryo formation rate was about 33% by suspension culture. The ratio of embryo germination was 60.9%, on the other side, the root formation rate was 74.3% in 1/2 MS continuously.

• Archives of Reconstructive Microsurgery
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• 제2권1호
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• pp.53-61
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• 1993

High level, major limb amputation above the wrist and ankle joint has some characteristic problems because of the large muscle mass and poor potential for nerve regeneration. As an adjunct method to reduce warm ischemic time prior to bony stabilization, temporary vascular shunting by simple catheterization has been performed, which has not been associated with any significant complication and has improved on success rate in replantation surgery. The authors have experienced 198 cases replantation of amputated limbs and digits from September, 1983 at the Korea medical center, Guro hospital, of which, 13 cases of successful replantation of amputated limbs by avulsion injury above the wrist and ankle joint level, which is generally considered as contraindication, were followed up for average 4.5 years(minimum 1.5 to maximum 8.4 years) on terms of survival rate, function and appearance. The clinical analysis upon these cases is to be presented with review of references.

• 한국태양에너지학회:학술대회논문집
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• 한국태양에너지학회 2009년도 춘계학술발표대회 논문집
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• pp.162-169
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• 2009

In summer electrical energy is consumed in very high rate. It is used to operate conventional air conditioning system. Hot and humid air can germinate mould spores, encourage ill health, and create physiological stress (discomfort). Dehumidifier solar cooling effect is the one alternative solution saving electrical energy. We use surplus heat energy in the summer, to get cooling effect and then to get human reach to comfort condition. These devices have two system, dehumidifier and regeneration system. This paper will be focus in dehumidifier system. Dehumidifier system use for absorbing moisture in the air and decreasing air temperature. When the liquid desiccant as strong solution contact with the vapor air in the packed tower, it works. The heat and mass transfer performances of flow pattern in the packed tower of dehumidifier are analyzed and compared in detail. In this experiment was introduced, the flow patterns are parallel flow and counter flow. The performance of these flow patterns will calculate from air side. Which is the best flow pattern that gave huge mass transfer rate? The proposed dehumidifier flow pattern will be helpful in the design and optimization of the dehumidifier solar cooling system.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.173-173
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• 2017

Phalaenopsis orchid is one of the most important flowers in flower industry. We conducted an experiment to find out the response of different concentration of chlorocholine chloride (CCC) for the in vitro regeneration of protocorm like bodies (PLBs) of Phalaenopsis 'Fmk02010'. We used five different concentrations of CCC and these were 0 (control), 0.01, 0.1, 1 and $10mgL^{-1}$ in modified MS medium and cultured for 42 days. We added two major salts ammonium nitrate ($412.5mgL^{-1}$) and potassium nitrate ($950.0mgL^{-1}$) to the MS medium for the modification. Maximum numbers of PLBs were found from media with $0.01mgL^{-1}$ of CCC (15.667) and maximum fresh weight (0.211 g) as well. The 100% PLB formation rate was also found from $0.01mgL^{-1}$ of CCC. We found 58.83% variation in number of PLBs ($R^2=0.5883$) and 47.44% variation in fresh weight ($R^2=0.4744$) to the different CCC concentrations. Our study suggested that increase in the CCC concentration negatively affect the PLBs organogenesis of Phalaenopsis. We can suggest that the addition of very low concentration of CCC in plant culture medium can increase the number, formation rate and fresh weight of PLBs of Phalaenopsis.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.177-177
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• 2017

Phalaenopsis orchid is one of the most important flowers in flower industry. We conducted an experiment to find out the response of different concentration of chlorocholine chloride (CCC) for the in vitro regeneration of protocorm like bodies (PLBs) of Phalaenopsis 'Fmk02010'. We used five different concentrations of CCC and these were 0 (control), 0.01, 0.1, 1 and $10mgL^{-1}$ in modified MS medium and cultured for 42 days. We added two major salts ammonium nitrate ($412.5mgL^{-1}$) and potassium nitrate ($950.0mgL^{-1}$) to the MS medium for the modification. Maximum numbers of PLBs were found from media with $0.01mgL^{-1}$ of CCC (15.667) and maximum fresh weight (0.211 g) as well. The 100% PLB formation rate was also found from $0.01mgL^{-1}$ of CCC. We found 58.83% variation in number of PLBs ($R^2=0.5883$) and 47.44% variation in fresh weight ($R^2=0.4744$) to the different CCC concentrations. Our study suggested that increase in the CCC concentration negatively affect the PLBs organogenesis of Phalaenopsis. We can suggest that the addition of very low concentration of CCC in plant culture medium can increase the number, formation rate and fresh weight of PLBs of Phalaenopsis.