• Title/Summary/Keyword: Reference yield

Search Result 271, Processing Time 0.022 seconds

Purification of type B Staphylococcal enterotoxin (Staphylococcus aureus에서 생성된 Enterotoxin B의 분리 및 정제)

  • 이정희;신현길;김종배;한재수
    • Journal of Food Hygiene and Safety
    • /
    • v.3 no.2
    • /
    • pp.75-81
    • /
    • 1988
  • Various methods such as lel-mtration on Sephadex G-SO, 75, 100 Sephacry, and Ultro gel, and lon-exchanle chromatoaraphy on Amberilte and carboxymethyl (CM)-cellulose, and Fast Protein liquid Chromatolraphy (FPLC) were applied for the purification of enterotoxin B from Staphylococcus aureus ATCC 14458 and compared one another. lon-exchanle chromatography on Amberllte resin was good enough to remove non-entrotoxln materials in culture, convlnient to use and fast although tbe purity was less tban 70%. However, CM-cellulose showed to be better purity and yield tban those of Amberilte resin. The yields of these two resins for ion-exchange cbromatograpby were about 70% and 75%, respectively. When the gel-filtration methods on Sepbadex G-50, 75, 100, Sepbacryl, and Ultro lei were applied, the purities were about 90%. FPLC was found to be tbe most efficient metbod in terms of purity (96%) and speed. For the purification of sample with large volume, particularly, tbe combined metbod, gel-mtration after Amberlite can be also used efficiently. Tbe purified toxin was found to be identical to type B enterotoxin used for reference standard by Oucbterlony immunodiffusion test.

  • PDF

A Study on the Efficiency Analysis of IT Service Companies Using Meta Frontier and the Determinants of Efficiency Using Tobit Model (Meta Frontier를 이용한 국내 IT서비스기업의 효율성 분석 및 Tobit 모형을 이용한 효율성 결정요인 분석에 대한 연구)

  • Shin, Minsoo;Park, Jiyong
    • Journal of Information Technology Services
    • /
    • v.16 no.4
    • /
    • pp.15-31
    • /
    • 2017
  • This study analyzes 45 Korea IT service companies from 2012 to 2016 using DEA analysis. Large enterprises, medium enterprises and small and medium enterprises (SMEs). CCR model and BCC model were used for efficiency analysis. Among the various analytical objects, the decision objects which yield the maximum output with minimum input are compared with other analysis objects. The relative inefficiency was measured through this, and Technical Efficiency (TE), Pure Technology Efficiency (PTE), Scale Efficiency (SE), scale profit, reference frequency were analyzed. Also, we analyzed the Technology Gap Ratio (TGR), which is the distance between production function and Meta-Frontier for each firm, using Meta-Frontier analysis. Finally, the Tobit model is used to analyze the sources of efficiency and inefficiency. The inputs are assets, capital, and employees, and the output factor is sales. The analysis shows that large firms are achieving technological achievements more efficiently than small and medium enterprises. As a result, medium-sized enterprises and SMEs can improve efficiency overall through efficient operation of workforce and appropriate combination of inputs such as assets and capital. Also, as a result of the influence factor analysis, it was found that the ratio of the managed asset ratio and the management cost ratio were significant factors influencing the efficiency of the IT service companies. This study suggests the efficiency analysis using DEA for many Korea IT service companies. Inefficient parts of each company are classified according to size and technology. Also, we identify the most efficient companies and analyze the causes of those companies whose profits are lower than their size.

Cereal Scab Causing Mycotoxicoses in Korea and Present Status of Mycotoxin Researches (한국(韓國)에서의 진균중독증(眞菌中毒症)을 일으키는 맥류(麥類) 붉은 곰팡이병 및 진균독소(眞菌毒素) 연구(硏究)의 현황(現況))

  • Chung, Hoo-Sup
    • The Korean Journal of Mycology
    • /
    • v.3 no.1
    • /
    • pp.31-36
    • /
    • 1975
  • In 1963, a severe epidemic of cereal scab caused by Gibberella zeae occurred in southern Korea and to a less extent in central and northern Korea. In some areas losses were $80{\sim}100$ percent. The epiphytotic was due to heavy rainfall during the heading and flowering season which provided a favorable environment for this severe epidemic. Yield losses resulted a great social problem because of the resultant food and feed grain shortage, lose of seeds for planting the following crops and mycotoxicoses to man and animals. In the same year, a nationwide research committee was organized including plant pathologists, microbiologists, agronomists and biochemists under the juristiction of the Ministry of Agriculture and Forestry. The committee initiated research on etiology, epidemiology, and control of the disease and on the toxic effect of infected grains to man and animals. The present paper will review some research carried out in Korea on cereal scab with special reference to epidemiology and mycotoxicoses to animals and man. In addition, the present status of research in Korea on aflatoxins in foods and toxic moldy rice will be briefly reviewed.

  • PDF

A Zinc Porphyrin Sensitizer Modified with Donor and Acceptor Groups for Dye-Sensitized Solar Cells

  • Lee, Seewoo;Sarker, Ashis K.;Hong, Jong-Dal
    • Bulletin of the Korean Chemical Society
    • /
    • v.35 no.10
    • /
    • pp.3052-3058
    • /
    • 2014
  • In this article, we have designed and synthesized a novel donor-${\pi}$-acceptor (D-${\pi}$-A) type porphyrin-based sensitizer (denoted UI-5), in which a carboxyl anchoring group and a 9,9-dimethyl fluorene were introduced at the meso-positions of porphyrin ring via phenylethynyl and ethynyl bridging units, respectively. Long alkoxy chains in ortho-positions of the phenyls were supposed to reduce the degree of dye aggregation, which tends to affect electron injection yield in a photovoltaic cell. The cyclic voltammetry was employed to determine the band gap of UI-5 to be 1.41 eV based on the HOMO and LUMO energy levels, which were estimated by the onset oxidation and reduction potentials. The incident monochromatic photon-to-current conversion efficiency of the UI-5 DSSC assembled with double-layer (20 nm-sized $TiO_2$/400 nm-sized $TiO_2$) film electrodes appeared lower upon overall ranges of the excitation wavelengths, but exhibited a higher value over the NIR ranges (${\lambda}$ = 650-700 nm) compared to the common reference sensitizer N719. The UI-5-sensitized cell yielded a relatively poor device performance with an overall conversion efficiency of 0.74% with a short circuit photocurrent density of $3.05mA/cm^2$, an open circuit voltage of 0.54 mV and a fill factor of 0.44 under the standard global air mass (AM 1.5) solar conditions. However, our report about the synthesis and the photovoltaic characteristics of a porphyrin-based sensitizer in a D-${\pi}$-A structure demonstrated a significant complex relationship between the sensitizer structure and the cell performance.

Preparation of Metal-p-aminobenzyl-DOTA Complex Using Magnetic Particles for Bio-tagging in Laser Ablation ICP-MS

  • Yoon, S.Y.;Lim, H.B.
    • Bulletin of the Korean Chemical Society
    • /
    • v.33 no.11
    • /
    • pp.3665-3670
    • /
    • 2012
  • Metal-p-$NH_2$-Bn-DOTA (paraammionobenzyl-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid: ABDOTA) complex was synthesized and purified for bio-tagging to quantify biological target materials using laser ablation (LA)-ICP-MS. Since the preparation of a pure and stable tagging complex is the key procedure for quantification, magnetic particles were used to purify the synthesized metal-ABDOTA complex. The magnetic particles immobilized with the complex attracted to a permanent magnet, resulting in fast separation from free un-reacted metal ions in solution. Gd ions formed the metal-complex with a higher yield of 64.3% (${\pm}3.9%$ relative standard deviation (RSD)) than Y ions, 52.3% (${\pm}2.5%$ RSD), in the pH range 4-7. The complex bound to the magnetic particles was released by treatment with a strong base, of which the recovery was 81.7%. As a reference, a solid phase extraction (SPE) column packed with Chelex-100 resin was employed for separation under similar conditions and produced comparable results. The tagging technique complemented polydimethylsiloxane (PDMS) microarray chip sampling in LA-ICP-MS, allowing determination of small sample volumes at high throughputs. For application, immunoglobulin G (IgG) was immobilized on the pillars of PDMS microarray chips and then tagged with the prepared Gd complex. IgG could then be determined through measurement of Gd by LA-ICP-MS. A detection limit of 1.61 ng/mL (${\pm}0.75%$ RSD) for Gd was obtained.

Analysis of the Bearing Behavior of a Tripod Bucket Installed in Clay (점성토 지반에 설치된 Tripod 버켓기초의 지지거동 분석)

  • Kim, Sung-Ryul;eong, Jae-Uk;Oh, Myounghak;Kwon, Osoon
    • KSCE Journal of Civil and Environmental Engineering Research
    • /
    • v.32 no.3C
    • /
    • pp.105-111
    • /
    • 2012
  • Bucket foundations, which are used in the foundations of offshore wind turbines, should be able to withstand large amounts of horizontal and moment loads. Tripod bucket foundation, which combines three single buckets, has been used to increase horizontal and moment capacities. This study performed numerical analysis using ABAQUS (2010), to analyze the group effect and the bearing capacity of a tripod bucket in clay. Parametric studies were performed varying the bucket spacing ratio S/D (S=spacing between the centers of the bucket and the tower; D=diameter of the bucket) and depth ratio L/D (L=embedded length of skirt). The applied constitutive models were a linear elastic perfectly plastic model with Tresca yield criteria for normally consolidated clay and an elastic model for buckets. Loading in the vertical, horizontal, and moment directions was simulated with an increase in each movement at a reference point. The bearing behavior and the capacities of a single and a tripod bucket were compared. Capacity evaluation method of the tripod bucket was suggested using the capacity of a single bucket.

Load-carrying capacity degradation of reinforced concrete piers due to corrosion of wrapped steel plates

  • Gao, Shengbin;Ikai, Toyoki;Ni, Jie;Ge, Hanbin
    • Steel and Composite Structures
    • /
    • v.20 no.1
    • /
    • pp.91-106
    • /
    • 2016
  • Two-dimensional elastoplastic finite element formulation is employed to investigate the load- carrying capacity degradation of reinforced concrete piers wrapped with steel plates due to occurrence of corrosion at the pier base. By comparing with experimental results, the employed finite element analysis method is verified to be accurate. After that, a series of parametric studies are conducted to investigate the effect of corrosion ratio and corrosion mode of steel plates located near the base of in-service pier P2 on load-carrying capacity of the piers. It is observed that the load-carrying capacity of the piers decreases with the increase in corrosion ratio of steel plates. There exists an obvious linear relationship between the load-carrying capacity and the corrosion ratio in the case of even corrosion mode. The degradation of load-carrying capacity resulted from the web's uneven corrosion mode is more serious than that under even corrosion mode, and the former case is more liable to occur than the latter case in actual engineering application. Finally, the failure modes of the piers under different corrosion state are discussed. It is found that the principal tensile strain of concrete and yield range of steel plates are distributed within a wide range in the case of slight corrosion, and they are concentrated on the column base when complete corrosion occurs. The findings obtained from the present study can provide a useful reference for the maintenance and strengthening of the in-service piers.

Application of Highland Kimchi Cabbage Status Map for Growth Monitoring based on Unmanned Aerial Vehicle

  • Na, Sang-Il;Park, Chan-Won;Lee, Kyung-Do
    • Korean Journal of Soil Science and Fertilizer
    • /
    • v.49 no.5
    • /
    • pp.469-479
    • /
    • 2016
  • Kimchi cabbage is one of the most important vegetables in Korea and a target crop for market stabilization as well. In particular Kimchi cabbages in a highland area are very sensitive to the fluctuations in supply and demand. Yield variability due to growth conditions dictates the market fluctuations of Kimchi cabbage price. This study was carried out to understand the distribution of the highland Kimchi cabbage growth status in Anbandeok. Anbandeok area in Gangneung, Gangwon-do, Korea is one of the main producing districts of highland Kimchi cabbage. The highland Kimchi cabbage status map of each growth factor was obtained from unmanned aerial vehicle (UAV) imagery and field survey data. Six status maps include UAVRGB image map, normalized difference vegetation index (NDVI) distribution/anomaly map, Crop distribution map, Planting/Harvest distribution map, Growth parameter map and Growth disorder map. As a result, the highland Kimchi cabbage status maps from May 31 to Sep. 6 in 2016 were presented to show spatial variability in the field. The benefits of the highland Kimchi cabbage status map can be summarized as follows: crop growth monitoring, reference for field observations and survey, the relative comparison of the growth condition in field scale, evaluation of growth in comparison of average year, change detection of annual crops or planting areas, abandoned fields monitoring, prediction of harvest season etc.

Surface Analysis of Fluorine-Plasma Etched Y-Si-Al-O-N Oxynitride Glasses

  • Lee, Jung-Ki;Hwang, Seong-Jin;Lee, Sung-Min;Kim, Hyung-Sun
    • Proceedings of the Materials Research Society of Korea Conference
    • /
    • 2009.05a
    • /
    • pp.38.1-38.1
    • /
    • 2009
  • Plasma etching is an essential process for electronic device industries and the particulate contamination during plasma etching has been interested as a big issue for the yield of productivity. The oxynitride glasses have a merit to prevent particulate contamination due to their amorphous structure and plasma etching resistance. The YSiAlON oxynitride glasses with increasing nitrogen content were manufactured. Each oxynitride glasses were fluorine-plasma etched and their plasma etching rate and surface roughness were compared with reference materials such as sapphire, alumina and quartz. The reinforcement mechanism of plasma etching resistance of the YSiAlON glasses studied by depth profiling at plasma etched surface using electron spectroscopy for chemical analysis. The plasma etching rate decreased with nitrogen content and there was no selective etching at the plasma etched surface of the oxynitride glasses. The concentration of silicon was very low due to the generation of SiF4 very volatile byproduct and the concentration of aluminum and yttrium was relatively constant. The elimination of silicon atoms during plasma etching was reduced with increasing nitrogen content because the content of the nitrogen was constant. And besides, the concentration of oxygen was very low on the plasma etched surface. From the study, the plasma etching resistance of the glasses may be improved by the generation of nitrogen related structural groups and those are proved by chemical composition analysis at plasma etched surface of the YSiAlON oxynitride glasses.

  • PDF

Study on Section Properties of Asymmetric-Sectioned Vessels (선박의 비대칭 단면 특성에 대한 연구)

  • Choung, Joon-Mo;Kim, Young-Hun
    • Journal of the Society of Naval Architects of Korea
    • /
    • v.47 no.6
    • /
    • pp.843-849
    • /
    • 2010
  • This paper presents definition of symmetry of a ship section where three symmetries are proposed: material, geometric, and load symmetries. Precise terminologies of centroid, moment plane, and neutral axis plane are also defined. It is suggested that force vector equilibrium as well as force equilibrium are necessary condition to determine new position of neutral axis due to translational and rotational mobility. It is also stated that new reference datum of ENMP(elastic neutral moment plane), PNMP(fully plastic moment plane), ENAP(elastic neutral axis plane), and INAP(inelastic neutral moment plane) are required to define asymmetric section properties such as second moment of area, elastic section modulus, yield moment, fully plastic moment, and ultimate moment. Since collision-induced damage and flooding-induced biaxial bending moment produce typical asymmetry of section, the section properties are calculated for a typical VLCC. Geometry asymmetry is determined from ABS and DNV rules and two moment planes of 0/30 degs are assumed for load asymmetry. It is proved that the property reduction ratios directly calculated from second moment of area are usually larger than area reduction ratio. Reduction ratio of ultimate moment capacity shows almost linearly proportional to area reduction ratio. Mobility of elastic and inelastic neutral axis planes is visually provided.