• Title/Summary/Keyword: Reference state

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Soil-water Characteristic Curve Assessment Using a Reference State Concept (비교상태 개념을 이용한 흙-수분 특성곡선 평가)

  • 성상규;이인모;이형주;조국환
    • Journal of the Korean Geotechnical Society
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    • v.20 no.5
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    • pp.27-35
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    • 2004
  • The goal of this study is to investigate the feasibility of the reference state approach in determining the generalized soil-water characteristic curve that is essential fur characterization of unsaturated soil behavior. The soil-water characteristic curves are obtained from a number of specimens of fine-grained residual soils compacted with different void ratios. Based on the experimental test results, the feasibility of using the liquid limit state as the reference state for predicting the soil-water characteristic curve is verified. Finally, through the regression analysis of experimental data using the equation of Fredlund and Xing (1994), a reliable method is proposed to predict the generalized soil-water characteristic curve of fine-grained residual soils using the liquid limit state as the reference state.

Establishment and application of a solid-phase blocking ELISA method for detection of antibodies against classical swine fever virus

  • Cao, Yuying;Yuan, Li;Yang, Shunli;Shang, Youjun;Yang, Bin;Jing, Zhizhong;Guo, Huichen;Yin, Shuanghui
    • Journal of Veterinary Science
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    • v.23 no.5
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    • pp.32.1-32.11
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    • 2022
  • Background: Classical swine fever (CSF) is a severe infectious disease of pigs that causes significant economic losses to the swine industry. Objectives: This study developed a solid-phase blocking enzyme-linked immunosorbent assay (spbELISA) method for the specific detection of antibodies against the CSF virus (CSFV) in porcine serum samples. Methods: A spbELISA method was developed based on the recombinant E2 expressed in Escherichia coli. The specificity of this established spbELISA method was evaluated using reference serum samples positive for antibodies against other common infectious diseases. The stability and sensitivity were evaluated using an accelerated thermostability test. Results: The spbELISA successfully detected the antibody levels in swine vaccinated with the C-strain of CSFV. In addition, the detection ability of spbELISA for CSFV antibodies was compared with that of other commercial ELISA kits and validated using an indirect immunofluorescence assay. The results suggested that the spbELISA provides an alternative, stable, and rapid serological detection method suitable for the large-scale screening of CSFV serum antibodies. Conclusions: The spbELISA has practical applications in assessing the vaccination status of large pig herds.

Optical Implementation of Improved IPA Model Using Hierarchical Recognition Algorithm (계층적 인식 알고리즘을 이용한 개선된 패턴상호연상모델의 광학적 구현)

  • 하재홍;김성용;김수중
    • Journal of the Korean Institute of Telematics and Electronics A
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    • v.31A no.7
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    • pp.55-62
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    • 1994
  • Interpattern association (IPA) model which the interconnection weight matrix(IWM) is constructed by the association between patterns is effective in similar pattern recognitions. But, if the number of reference patterns is increased, the ability of recognition is decreased. Using a hierarchical recognition algorithm which adopts the tree search strategy, we classified reference patterns into sub-groups by similarity. In IPA model, if input includes random noise we make it converge to reference pattern by means of input includes random noise we make it converge to reference pattern by means of increasing the number of pixels of prohibited state in IWM. In relation to reference patterns the pixel of prohibited state made partially prohibited state of no connected state using which is not included common and feature regions by each reference patterns.

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Estimation of Generalized Soil-Water Characteristic Curves Using Liquid Limit State (액성한계상태를 이용한 흙-수분 특성곡선의 평가)

  • Sung, Sang-Gyu;Lee, In-Mo
    • Proceedings of the Korean Geotechical Society Conference
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    • 2004.03b
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    • pp.146-153
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    • 2004
  • The goals of this study are to investigate the feasibility of the reference state approach in determining the generalized soil-water characteristic curve that is essential for characterization of unsaturated soil behavior. The soil-water characteristic curves are obtained from a number of specimens of fine-grained residual soils compacted with different void ratios. Based on the experimental test results, the feasibility of using the liquid limit state as the reference state for predicting the soil-water characteristic curve are verified. Finally, through the regression analysis of experimental data using the equation of Fredlund and Xing (1994), a reliable method is proposed to predict the generalized soil-water characteristic curve of fine-grained residual soils using the liquid limit state as the reference.

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Study of the Application of Gel Electrolyte in the Reference Electrode of $Cu/CuSO_4$

  • Lin, Cunguo;Xu, Likun;Liu, Yang
    • Corrosion Science and Technology
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    • v.7 no.3
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    • pp.179-181
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    • 2008
  • With nano-$SiO_2$ and sulphate acid, a kind of colloid electrolyte is synthesized by sol-gel method. It is placed outside the reference electrode as a layer of gel electrolyte so as to decrease the leaching of $Cu^{2+}$ and increase the life of the reference electrode. The performance of the gel electrode in simulating soil solution is measured as follows: the potential of the electrodes ranging from 60 mV to 80 mV (vs. SCE) with potential variation no more than $\pm10mV$, enough resistance to polarization. The $Cu^{2+}$ effusion rate of the reference electrode without gel electrolyte is 3 times that with colloid electrolyte, which means that gel electrolyte can extend the life of the reference electrode significantly.

An Indirect Model Reference Adaptive Fuzzy Control for SISO Takagi-Sugeno Model

  • Cho, Young-Wan;Park, Chang-Woo;Lee, Ki-Chul;Park, Mignon
    • Transactions on Control, Automation and Systems Engineering
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    • v.3 no.1
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    • pp.32-42
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    • 2001
  • In this paper, a parameter estimator is developed for the plant model whose structure is represented by the Takagi-Sugeno model. The essential idea behind the on-line estimation is the comparison of the measured stated with the state of an estimation model whose structure is the same as that of the parameterized model. Based on the parameter estimation scheme, and indirect Model Reference Adaptive Fuzzy control(MRAFC) scheme is proposed to provide asymptotic tracking of a reference signal for the systems with uncertain for slowly time-varying parameters. The developed control law and adaptive law guarantee the boundedness of all signals in the closed-loop systems. In addition, the plant state tracks the state of the reference model asymptotically with time for any bounded reference input signal.

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Selection of Stable Reference Genes for Real-Time Quantitative PCR Analysis in Edwardsiella tarda

  • Sun, Zhongyang;Deng, Jia;Wu, Haizhen;Wang, Qiyao;Zhang, Yuanxing
    • Journal of Microbiology and Biotechnology
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    • v.27 no.1
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    • pp.112-121
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    • 2017
  • Edwardsiella tarda is a gram-negative pathogenic bacterium in aquaculture that can cause hemorrhagic septicemia in fish. Many secreted proteins have already been identified as virulent factors of E. tarda. Moreover, since virulent phenotypes are based on the expression regulation of virulent genes, understanding the expression profile of virulent genes is important. A quantitative RT-PCR is one of the preferred methods for determining different gene expressions. However, this requires the selection of a stable reference gene in E. tarda, which has not yet been systematically studied. Accordingly, this study evaluated nine candidate reference genes (recA, uup, rpoB, rho, topA, gyrA, groEL, rpoD, and 16S rRNA) using the Excel-based programs BestKeeper, GeNorm, and NormFinder under different culture conditions. The results showed that 16S rRNA was more stable than the other genes at different culture growth phases. However, at the same culture time, topA was identified as the reference gene under the conditions of different strains, different culture media, and infection, whereas gyrA was identified under the condition of different temperatures. Thus, in experiments, the expression of gapA and fbaA in E. tarda was analyzed by RT-qPCR using 16S rRNA, recA, and uup as the reference genes. The results showed that 16S rRNA was the most suitable reference gene in this analysis, and that using unsuitable reference genes resulted in inaccurate results.

The Study on Digital Reference Services of University Libraries in Korea (대학도서관의 디지털 참고봉사에 관한 연구)

  • Kim Dar-Sig
    • Journal of Korean Library and Information Science Society
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    • v.36 no.2
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    • pp.241-262
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    • 2005
  • This study examines current state of digital reference service on the homepage of university library in Korea. Among 236 university libraries. only $36.9\%$ (87) provide digital reference services. Digital reference services was weak as face-to-face reference services in Korea. Based on the research finding, revealed current state and related problems, and a few suggestions are provided for digital reference service system.

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Method of Making the Distribution of Voxels Uniform within the Volumetric 3D image Space

  • Lin, Yuanfang;Liu, Xu;Xie, Xiaoyan;Liu, Xiangdong;Li, Haifeng
    • 한국정보디스플레이학회:학술대회논문집
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    • 2008.10a
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    • pp.1138-1141
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    • 2008
  • By defining a uniform reference point array corresponding to the 3D voxel array and abandoning voxels whose deviations from their respective reference points exceed a given tolerance, the distribution of voxels within the volumetric 3D image space gets uniform, effects of non-uniform distribution upon the image reconstructing are eased.

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Investigation of the steel rebar corrosion using embeddable solid state reference electrode in marine environments (해양 환경에서 매립형 고체 기준 전극을 사용한 철근 부식 조사)

  • Subbiah, Karthick;Park, TaeJoon;Lee, Han-Seung
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2022.04a
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    • pp.57-58
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    • 2022
  • Reinforced concrete (RC) structures play a significant role in the construction industries. An embeddable solid-state reference electrode (ESSRE) was used to evaluate the corrosion status of steel rebar in the concrete of various cover thicknesses that exposed to the maritime environment (3.5 % NaCl) in this study. From the open circuit potential measurement (OCP), the passive state, the corrosion uncertainty, and the 90% probability of corrosion state of the steel rebars in the concrete were monitored by ESSRE. From the electrochemical impedance spectroscopy (EIS) method, severe corrosion was observed at the exposure period of 1510, 1847, 2350, and 3020 h for C10, C15, C20, and C30 concrete, respectively. The results confirm that the ESSRE can be useful to identify the corrosion occurrence and severe corrosion of steel rebar embedded in different cover depth concrete structures.

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